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형광현미경을 이용한 음용 지하수내 배양불능 세균의 관찰 및 정량적 평가 (The Observation and a Quantitative Evaluation of Viable but Non-Culturable Bacteria in Potable Groundwater Using Epifluorescence Microscopy)

  • 김인기;;황경숙
    • 미생물학회지
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    • 제38권3호
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    • pp.180-185
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    • 2002
  • 직접생균수측정법(direct viable count; DVC)과 평판법(p1ate count; PC)을 이용하여 시판되고 있는 먹는샘물, 약수, 도시 인근 지역과 축산농가 밀집지역의 음용 지하수로부터 채수된 시료에 대하여 정략적 평가를 실시하였다. DVC법에 의한 생균수는 전균수(total direct count: TDC)의 약 30~80%, 평판법에 의한 생균수는 DVC의 약1~30%로 나타났다. 이와 같은 결과는 지하수내에 배양 불가능한(viable but non-culturable: VBNC)세균이 높은 비율로 존재함이라고 판단된다. 한편, 통상농도의 영양배지(nutrient broth; NB)와 이를 $10^-2 -2/배로 희석한 영양배지(diluted nutrient broth; DNB)및 R2A배지를 이용하여 계수한 결과, 통상농도의 NB배지에 비해 저영양배지인 DNB와 R2A에서 2~50배 이상 높은 계수치를 나타내었다. 이와 같은 결과로부터 지하수와 같이 빈영양한 환경 내에는 통상농도의 NB배지에서는 중식이 현저히 저해되고 저영양배지에서 중식 가능한 저영양세균이 다수 분포해 있음이라 판단되었다.

한국인의 지문 (Dermatoglyphic Traits in Koreans)

  • Park, Kyung-Sook;Cheon, Hee-Yeong
    • 한국동물학회지
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    • 제27권4호
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    • pp.213-220
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    • 1984
  • 한국인 2,925명 (남자:804, 여자:2,121)에 대하여 지문형과 pattern intensity index를 분석하였다. 지문형의 출현빈도는 남녀의 성차가 있었다. Whorls (남자:53.6%, 여자:45.7%)와 Radial loops (남자:2.9%, 여자:2.2%)는 남자에서 높이 나타났고, 반면 Archs (남자:1.9%, 여자:2.9%)와 Ulnar loops (남자:41.6%, 여자:49.2%)는 여자에서 출현빈도가 높았다. Pattern intensity index는 남자가 15.2가, 여자는 14.3으로 남자에서 높았다. Polygene model의 유전을 하는 양적 형질인 지문 총 융선수를 776명 (남자:426, 여자:250)에 대하여 계산한 결과 총 융선수는 남자가 140.4$\\pm$41.2로 여자의 129.8$\\pm$40.6보다 높았다. 지금까지 다른나라에서 보고된 총 융선수는 인종에 따라 121$\\sim$170으로 인종적 차가 있었다. 총 융선수와 지능지수와의 상관관계는 없었다(r=-0.0027, N=343).

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A Modified Computing Algorithm for Raking Ratio Estimation Subject to Partial Marginal Information

  • Son, Chang Kyoon
    • Communications for Statistical Applications and Methods
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    • 제11권2호
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    • pp.419-433
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    • 2004
  • We suggest the modified computing algorithm for raking ratio estimation under the assumption that the population total is partially known, and the sample total is completely known about survey variable in contingency table. We show that the proposed estimation procedure is useful to estimate the population cell count in this situation through an empirical study.

Evaluation of the $HApS^{TM}$ Method for the Enumeration of Aerobic Microorganisms and Coliforms in Retailed Meat Samples in Korea

  • Keun-Seok Seo;Wonki Bae;So-Hyun Kim;Nam-Hoon Kwon;Ji-Yeun Kim
    • 한국식품위생안전성학회지
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    • 제16권3호
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    • pp.194-199
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    • 2001
  • 국내에서 시판중인 230점(돈육샘플80점, 계육샘플 80점, 우육샘플 70점)의 식육 샘플에 대하여 미생물 오염수준 측정시 표준방법으로 통용되는 AOAC (the Association of Official Analytical Chemists)의 standard total aerobic count method 및 coliform count method와 국내에서 개발된 HApS$^{TM}$ (Hazard Analysis process System) method를 비교하였다. 돈육, 계육, 우육을 샘플군으로 하여 총세균수 및 대장균수를 표준방법과 HApS$^{TM}$방법으로 각각 측정하여 그 두 방법간의 상관관계를 조사하였다. 선형회귀분석 및 t-검정법의 통계적 방법을 사용하여 각 샘플군에서의 두 방법간의 상관계수(r) 및 회귀직선식을 얻었고, 각 샘플군에서 두 방법에 의한 각각의 표본평균이 주어진 유의수준($\alpha$=0.05)에서 유의한 차이를 보이지 않았다. 즉, 본 실험에 사용된 샘플에 대하여 표준방법과 HApS$^{TM}$ 방법간의 유의한 차이를 발견하지 못하였다. 그러므로 표준방법에 비하여 실험시간 및 실험방법에서 신속성 및 간편성을 지니는 HApS$^{TM}$방법은 축산식품의 총세균 및 대장균군 오염도 측정시에 표준방법을 대신할 수 있는 유용한 방법이라고 할 수 있다.

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매실(Prunus mume) 착즙액이 항균성과 생면의 저장성에 미치는 영향 (Effect of Maesil(Prunus mume) Juice on Antimicrobial Activity and Shelf-Life of Wet Noodle)

  • 이현애;남은숙;박신인
    • 한국식생활문화학회지
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    • 제18권5호
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    • pp.428-436
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    • 2003
  • The effect of addition with maesil(Prunus mume) juice for extending the shelf-life of wet noodle was investigated by measuring quality changes such as total microbial count and pH. The Prunus mume juice showed antimicrobial activities against Salmonella enteritidis, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, and Escherichia coli. When the wet noodles containing Prunus mume juice were kept at $4^{\circ}C$ for 20 days, it was showed that the drop of pH was not significantly occurred during the storage. Total microbial counts for control exceeded the initial putrefactive criterion level of $1.0{\times}10^6\;cfu/g$ at 16 days of storage at $4^{\circ}C$. However, total microbial count of wet noodles with the addition of 10%, 20% and 30% Prunus mume juice were $3.0{\times}10^2\;cfu/g,\;3.0{\times}10^2\;cfu/g,\;and\;1.5{\times}10^2\;cfu/g$, respectively, and these bacterial counts were still less than the criterion level ever at 20 days of storage. The addition of Prunus mume juice extended the shelf-life of wet noodle appreciably.

관절염 백선의 부종과 혈액에 미치는 천수근의 효능연구 (Study of Effects of Harpagophyti Radix on Edema and Blood of Rats with Arthritis)

  • 이정훈;김연섭
    • 동의생리병리학회지
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    • 제18권2호
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    • pp.446-450
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    • 2004
  • Objectives: In order to study the effects of Harpagophyti Radix in rats with arthritis induced by Freund's complete adjuvant. Methods : In this study, rats with arthritis induced by Freund's complete adjuvant were divided into four groups; Normal, Control, Sample A(Harpagophyti Radix 6mg/200g) and Sample B(Harpagophyti Radix 12mg/200g). After 1 week, separated searches were made on the change of edema rate, the count of WBC, the quantity of total protein, total bilirubin, GOT and GPT in the serum. Results: In the right plantar edema, both of Sample groups showed a decrease in comparison with Control group. In the count of WBC, the quantity of serum total protein and SGOT, Sample B showed a decrease with statistical significance in comparison with Control group. In the quantity of serum total bilirubin and SGPT, both of Sample groups did not show any harmful change in in comparison with Control group. Conclusion : Reviewing these experimental results, it appeared that Harpagophyti Radix showed the therapeutic effect of anti-inflammatation on adjuvant arthritis.

The impact of lymph node count on survival in gastric cancer

  • Ahn, Ha Rim;Han, Se Wung;Yang, Doo Hyun;Kim, Chan Young
    • 대한종양외과학회지
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    • 제14권2호
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    • pp.120-127
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    • 2018
  • Purpose: The purpose of this study was to determine the immunologic role of lymph node (LN) and stage migration by assessing LN count and metastatic LN count. Methods: A total of 2,117 patients with gastric adenocarcinoma located in the body and antrum who underwent distal/subtotal gastrectomy with D2 LN dissection between January 1, 1998 and December 31, 2008 were enrolled. LN count and number of metastases were determined in the N1 tier (area of D1 dissection) and N2 tier (area of D2 dissection). The lower and upper quartiles of LN counts in the same pN stage were grouped to compare the prognosis and LN positivity according to the LN tier. Results: Stage migration from N1 tier to N2 tier occurred in 3.2% of cases. The 5-year disease-specific survival rates of the upper and lower LN count groups within the N1 tier were 91.0% and 86.7% (P=0.01), respectively. LN positivity in the N2 tier of the lower LN count group was higher than that of the upper LN count group (14.1% vs. 8.2%, P<0.01). Stage migration in the N2 tier of the lower LN count group was also higher than that of the upper LN count group (4.6% vs. 1.8%, P<0.01). Conclusion: The lower LN count group had a decreased survival rate compared to that of the upper LN count group, suggesting that perigastric LN has an immunological defense role in weakening the disseminating power of metastatic tumor cells, as indicated by the LN count.

A Comparison of Complete Blood Cell Count in Canine Blood Samples Obtained from the Jugular Vein, Cephalic Vein and Lateral Saphenous Vein

  • An, Hyung-Mo;Song, Joong-Hyun;An, Su-Jin;Yu, Do-Hyeon;Kim, Young Joo;Han, Donghyun;Jung, Dong-In
    • 한국임상수의학회지
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    • 제36권6호
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    • pp.314-318
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    • 2019
  • The purpose of this study was to compare the results of complete blood cell count (CBC) of blood samples collected from the jugular vein, cephalic vein and lateral saphenous vein and to find out if there were clinically significant differences. Total of 40 dogs were tested. CBC tests were conducted with blood samples obtained from the jugular vein, cephalic vein and lateral saphenous vein and manual differential count was performed to accurately distinguish the white blood cell (WBC) types. The results were analyzed using Repeated Measures ANOVA and posthoc test was conducted using the least significant difference method. As a result, there was a statistically significant difference (P < 0.05) in the total WBC and monocyte count. The post-hoc test of total WBC counts revealed a significant difference between the jugular vein and cephalic vein, and the jugular vein and lateral saphenous vein. For monocyte counts, a significant difference was observed between the jugular vein and lateral saphenous vein.

Effect of dietary Achyranthes japonica extract on growth performance of growing pigs and absorption rate of quercetin in blood

  • Md Mortuza Hossain;Hyung Suk Hwang;Minyeong Pang;Min-Koo Choi;In Ho Kim
    • Journal of Animal Science and Technology
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    • 제66권1호
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    • pp.103-114
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    • 2024
  • This study was done to investigate the effects of the incorporation of Achyranthes japonica extracts (AJE) in diet on the production parameters of growing pigs. Exp 1: Total, 105 crossbred pigs (average body weight: 24.47 ± 2.46 kg) were used in a 6-week feeding trial. Pigs (seven replicates, five pigs per pen) were allotted randomly to three treatments. Dietary treatments: CON (basal diet); basal diet with 0.025% AJE, and basal diet + 0.050% AJE). Growth performance, nutrient digestibility, fecal microbial count, and fecal noxious gas were assessed in this study. Average daily gain (ADG), average daily feed intake (ADFI), and gain to feed ratio (G:F) were not affected by the addition of up to 0.05% AJE. In the case of apparent total tract digestibility (ATTD), dry matter (DM), nitrogen (N), and digestible energy (DE) were not changed in 3rd and 6th weeks of the feeding trial through the addition of AJE up to 0.05% in the growing pig diet. In microbial count, Lactobacillus and Escherichia coli count at 3rd and 6th week was similar in all the treatment diets. The inclusion of AJE at levels up to 0.05% in growing pig diet had no effect on the production of NH3, H2S, acetic acid, and CO2 in the feces. After ending the Exp 1, a total of nine pigs were divided into three treatment groups. Treatment diets were included, TRT1, basal diet + powder quercetin 30 g; TRT2, basal diet + powder quercetin 150 g; TRT3, basal diet + powder quercetin 300g. Rate of absorption in blood was increased with the higher dose of quercetin. The results suggested incorporation of AJE up to 0.05% has no significant effect on ADG, ADFI, and G:F, as well as DM, N, and DE digestibility, fecal microbial count, and fecal noxious gas emission in growing pigs, even though no negative effect was found.

An Investigation into the Correlation between a Country's Total Olympic Medal Count, GDP, and Freedom Index through History

  • Snowberger, Aaron Daniel;Lee, Choong Ho
    • 한국정보통신학회:학술대회논문집
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    • 한국정보통신학회 2021년도 추계학술대회
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    • pp.495-498
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    • 2021
  • In 2021, Tokyo held the delayed 2020 Olympics in the ongoing COVID-19 pandemic. As with any Olympics, the final medal count was of particular interest. Since 2004, South Korea has consistently ranked in the top ten most successful countries for four straight Summer Olympics. However, in 2021, it fell short of a top ten finish for the first time since 2004. There may be many factors that affect the final medal count in any Olympics, and this study compares and looks for correlations between some of these factors including final medal count, GDP, population, size of Olympic contingent, and Freedom Index. The study pays particular attention to South Korea and other countries which have seen a dramatic change in Freedom Index score since 1972, when the index was first published.

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