• Proceedings of the Korean Society of Crop Science Conference
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• 2020.11a
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• pp.174-174
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• 2020

• Preventive Nutrition and Food Science
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• v.16 no.2
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• pp.184-188
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• 2011

The melatonin, total polyphenol contents, and DPPH radical scavenging activity were determined in alfalfa, chicory, rape, red kale and sunflower after germination for four days at $24{\pm}0.1^{\circ}C$. Compared with seeds, melatonin content was increased in all sprouts, at the highest level in red kale (2,502.9 pg/g, 5.6 times higher than seed) followed by rape (2,430.1 pg/g), chicory (2,037.7 pg/g), alfalfa (1,160.8 pg/g) and sunflower (768.2 pg/g) sprout, however, the addition of tryptophan (0.5 mM), the precursor of melatonin synthesis, did not show any desirable effect. Both polyphenol content and DPPH radical scavenging activity were substantially increased in chicory (8.7 mg/g, 66%), rape (10.7 mg/g, 51%) and red kale (11.0 mg/g, 53%) sprouts, but not in alfalfa and sunflower sprouts. Melatonin content per gram polyphenol (ng/g) was also increased in all sprouts through germination. Germination was effective in increasing melatonin in all seeds tested, while its effect on polyphenol content and DPPH radical scavenging activity was species dependent.

• Journal of the Korean Society of Clothing and Textiles
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• v.42 no.2
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• pp.269-277
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• 2018

This study extracted Artemisia capillaris Thunberg with distilled water and ethanol to investigate its antioxidant effect. We then investigated the possibility of producing nanofibers by an electrospinning process by adding the extracts to polyvinyl alcohol (PVA). The electrospinning method used a PVA concentration of 12wt.%, an applied voltage of 10kV, and a tip-to-collector distance of 15cm. Total polyphenol and total flavonoid contents were measured to verify the antioxidant activity of Artemisia capillaris Thunberg extracts (ACEs). The total polyphenol content of the distilled water extract and the ethanol extract were measured as 218.47 and 271.26mg/g, respectively, and the total flavonoid content of the distilled water extract and the ethanol extract were measured as 141.68 and 34.98mg/g, respectively. As the content of the ACEs in the PVA nanofibers increased, the Diameters of the nanofibers and the uniformity of the diameters decreased. The electrospinning process was fabricated in a relatively uniform form without beads, and the diameters of the nanofibers that were produced ranged from 340 to 390nm. The results of FT-IR, XRD and DSC analyses confirmed that the ACEs were well mixed with the PVA molecules and were electrospun.

• Preventive Nutrition and Food Science
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• v.14 no.1
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• pp.43-48
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• 2009

Current study investigated phytochemical compositions and antioxidant capacity of Korean leafy vegetables including chajogi, gomchwi, meowi and sseumbagwi. ${\beta}$-carotene, total soluble polyphenol, total flavonoids contents were determined, and antioxidant capacity were evaluated by various methods. ${\beta}$-carotene, total soluble polyphenol, total flavonoids contents in gomchwi were significantly higher (as much as 19 folds) compare to other vegetables (p<0.05). Sseumbagwi extract ($100{\mu}g/mL$) was removed 78.6% of superoxide radicals in xanthin-xanthin oxidase system measured by EPR. Gomchwi showed the highest nitrate scavenging activity as 94.3% at pH 1.2. In SOD-like activity, chajogi, gomchwi, and meowi were evaluated in the range of $12.6{\sim}24.5%$. All samples were revealed to prevent the reaction of ferrous chloride during 9 days, which were comparable ability with 125 mg/100 g of ${\alpha}$-tocopherol. The rich phytochemical contents of gomchwi and sseumbagwi influenced to high antioxidant capacity.

• Korean journal of food and cookery science
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• v.31 no.1
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• pp.91-97
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• 2015

The present study was performed to investigate antioxidant and anti-colon cancer activities of red cabbage (Brassica oleracea L. var. capitata f. rubra DC) according to the cooking conditions (raw, microwave, blanching and steaming). The contents of red cabbage extracts were determined as follow: total phenolic contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethy lbenzo-thiazoline-6-sulfonic acid) (ABTS), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, western blot analysis. The total contents of polyphenol and flavonoid of red cabbage were 20.27 mg GAE/g Dry weight ${\pm}0.03$ and $2.55{\pm}0.02mg$ RE/g Dry weight. In this study, the total contents of polyphenol were decreased to both microwave and steam cooking. Total antioxidant activity and growth inhibition of HCT116 human colon cancer cells were in the order of raw > microwaving > steaming cooking methods. These results indicate that red cabbage extracts might have antioxidant and anti-proliferative activity according to the cooking conditions.

• Preventive Nutrition and Food Science
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• v.15 no.3
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• pp.244-247
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• 2010

This study was performed to evaluate the effect of drying methods on the polyphenol contents and antioxidant activities of Hizikia fusiformis. The seaweed was dried using four different conditions: vacuum drying (VD) at $20^{\circ}C$ and $30^{\circ}C$ and hot-air drying (HD) at $40^{\circ}C$ and $60^{\circ}C$. After drying, the total phenol content, DPPH and nitrite scavenging activities of the water extracts were determined. The total phenol contents were significantly (p<0.05) higher in the vacuum dried samples than the hot-air dried samples. DPPH radical was scavenged 82.67% and 83.45% by VD-20 and VD-30, and 70.44% and 71.23% by HD-40 and HD-60, respectively. At pH 1.2, Hizikia fusiformis scavenged 87.43%, 88.45%, 72.14% and 73.45% of nitrite by the treatment VD-20, VD-30, HD-40 and HD-60, respectively. Interestingly, the nitrite scavenging activity was much more reduced at pH 3.0 than at pH 1.2. Through these experiments, we were able to show that the drying conditions affect the total phenol contents and antioxidant capacities of Hizikia fusiformis.

• Korean Journal of Food Science and Technology
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• v.38 no.3
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• pp.331-336
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• 2006

Effects of high temperature and pressure treatment on garlic (Allium sativum L.) treated at 110, 120, 130, 140, and $150^{\circ}C$ for 1, 2, 3, 4, and 5 hr were evaluated by investigating changes in antioxidant activities, and total polyphenol, total flavonoid, and 5-hydroxymethyl-2-furaldehyde (5-HMF) contents of garlic juice. Antioxidant activities, and total polyphenol, total flavonoid, and 5-HMF contents increased with increasing treated temperature and time. $IC_{50}$ (1.952 mg/g) was achieved at $150^{\circ}C$ for 4 hr, 50 times higher than untreated garlics. Highest total polyphenol content was 18.155 mg/g at $140^{\circ}C$ and 2 hr, seven times higher than untreated garlics. Highest total flavonoid content was $532.73\;{\mu}g/g$ at $150^{\circ}C$ and 1 hr, sixteen times higher than untreated garlics. 5-HMF content was highest (7,765.9 ppm) at $150^{\circ}C$ and 2 hr. Correlation coefficients among antioxidant activities, and total polyphenol, total flavonoid, and 5-HMF contents were very high (p<0.01).

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.6
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• pp.739-744
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• 2012

Cirsium japonicum (Gyonggido (GG), Gangwondo (GW), Jeollanamdo (JN) and Jejudo (JJ)) were investigated for their antioxidative components (total polyphenol and flavonoid contents) and their antioxidant activities (2,2-diphenyl-1-picrylhydrazyl) (DPPH), 2,2'-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP) and reducing power). Analysis of antioxidative components and activities showed significant (p<0.05) differences across samples from various regions (GG, GW, JN, JJ). Amounts of total polyphenol and flavonoid contents were in the order of GG${\geq}$GW>JJ>JN (p<0.05). The results of the antioxidative activity test of Cirsium japonicum showed that GG and GW had higher activities compared to others (p<0.05). Therefore, the results of the antioxidative activity test (DPPH, ABTS, FRAP and reducing power) were correlated with total polyphenol and flavonoid contents values.

• Korean Journal of Agricultural Science
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• v.50 no.2
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• pp.219-230
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• 2023

Calla lily is one of the most iconic and widely recognized ornamental plants. This study compared the extracts of 11 cultivars of domestic calla lily bred by the National Institute of Horticultural and Herbal Science for their total polyphenol and antioxidant activities. Eleven cultivars were evaluated for their growth and flowering characteristics as per the Manual for Agricultural Investigation Rural Development Administration (RDA) form. The antioxidant activities were measured using 2,2'-azinobis (3-ethylbebzothiazoloine-6-sulfonic acid)-diammounium salt (ABTS⁺) and 1,1-diphenyl-2-picrrylhydrazyl (DPPH) radical scavenging effect. The plants have an average height of 63.80 ± 5.4 cm, average flower diameter of 7.2 ± 1.1 cm, and width of 12.4 ± 1.7 cm. On average, the diameter and width of leaves were 33.7 ± 3.5 cm and 20.0 ± 1.4 cm, respectively. Extracts of flowers and leaves in the 11 cultivars of white calla lily were compared for their antioxidant activities and total polyphenol contents. ABTS⁺ and DPPH radical scavenging, which are indicative of antioxidant activity, were higher in flowers than in leaves. When comparing by cultivar, we found that 'White Egg' showed the highest antioxidant activity in both the flowers and the leaves. Additionally, we found that by part, the content of total polyphenols was highest in flowers, and by cultivar, it was highest in the 'Swan' and 'White Egg' cultivars. Furthermore, the days to flowering showed correlations with ABTS⁺ radical scavenging, total phenolic contents (TPC), and total flavonoid contents (TFC). Our results indicate that calla lily can be used as breeding material material according to its growth characteristics and as a natural antioxidant source.

• The Korea Journal of Herbology
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• v.36 no.1
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• pp.77-85
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• 2021

Objective : This study was designed to investigate effect of immature fruits from Citrus unshiu Markovich to mature them on antioxidative and antiaging-related enzyme activities in vitro. Methods : Fruits from Open field-cultivated C. unshiu Markovich (ripe and unripe fruits of Citri) made a purchase in Jeju island. We measured total polyphenol and flavonoid contents in mature C. unshiu Markovich (MC) and immature C. unshiu Markovich (IMC) 70% ethanol extract. DPPH free radical, ABTS radical, and hydroxyl radical scavenging activities in both extracts were determined. Also, reducing power, SOD-like activities, elastase inhibition, and collagenase inhibition activities of these extract were assayed at 5, 1, 0.5, and 0.1 mg/㎖. Results : The total polyphenol contents in the ethanol extract of IMC were higher than those of MC (26.99 ± 0.89 mg/TAEg). And total flavonoid contents in them were very similar to both extract (MC; 7.25 ± 0.4 mg/RUEg and IMC; 7.44 ± 0.18 mg/RUEg). DPPH free radical scavenging activities, ABTS radical scavenging capacity, reducing power, and SOD-like activities of IMC-treated group showed significant increase compared to those of MC group in all treated concentrations. Particularly, DPPH free radical scavenging and elastase inhibition activities in IMC-treated group at 5 mg/㎖ showed similar with positive control group. Besides, IMC extract at 5 mg/㎖ concentration surpassed positive control (BHA or Vit. C) in SOD-like activities and ABTS radical scavenging capacity. Conclusion : These results suggest that IMC ethanol extract may be used as a natural antioxidant and a antiaging material for development of functional foods and cosmeceuticals.