• 제목/요약/키워드: Total Flavonoids Content

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Physicochemical qualities, antioxidant compounds, and activities of six mini paprika cultivars

  • Baek, Seolji;Shin, Youngjae
    • 한국식품과학회지
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    • 제52권4호
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    • pp.377-384
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    • 2020
  • Paprika is a popular vegetable with high visual appeal and desirable flavor, the health benefits of which are increasingly attracting interest. In this study, the physical qualities, antioxidant content, and activities of six mini paprika cultivars were investigated. Both the edible part (flesh) and the by-products were studied. The average total phenolics and total antioxidant activities were higher in the flesh than in the by-products. The total flavonoids of the flesh and the byproducts were 16.41 and 37.80 mg/100 g FW, respectively. "YW glory" and "Raon yellow" flesh had the highest (245.52 mg/100 g FW) and lowest (179.96 mg/100 g FW) total phenolics among the six cultivars, respectively. However, the "RD glory" cultivar showed the highest total phenolic content (232.70 mg/100 g FW) among the by-product samples. The total phenolics in the flesh and by-products were highly correlated to the ABTS radical scavenging activity, with R=0.961 and 0.984, respectively.

배추 종간 잡종의 소포자배양에 의한 Double haploid 집단의 플라보노이드 함량 분석 (Analysis of flavonoids in double haploid population derived from microspore culture of F1 hybrid of Brassica rapa)

  • 서미숙;원소윤;강상호;김정선
    • Journal of Plant Biotechnology
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    • 제44권1호
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    • pp.35-41
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    • 2017
  • 본 연구에서는 유지형 배추인 LP08과 청경채형 LP21을 교배한 교잡종 $F_1$을 소포자 배양하여 유전적으로 고정된 계통을 획득하였다. 엽의 결각 유무 및 결각수에서 다양한 형태적 특징을 보이는 66개 고정계통을 대상으로 항암 및 항산화 물질로 알려진 플라보노이드의 함량을 분석하였다. 66개 고정 계통의 엽의 결각 유무와 플라보노이드 함량 간 상관관계는 관찰되지 않았다. 양친으로 사용된 LP08, LP21 그리고 결구 배추형 품종인 Chiifu를 대상으로 플라보노이드 분석을 실시한 결과, quercetin, naringenin, 및 kaempferol 함량의 다양한 분포가 관찰되었다. 또한 모계인 LP08에서 가장 높은 총 플라보노이드 함량을 나타내었고 결구 배추형인 Chiifu에서 가장 낮은 함량을 보였다. 66개 고정 계통을 대상으로 플라보노이드 함량을 분석한 결과, quercetin은 LP08 보다 높은 함량을 보이는 18 계통이 확인되었으나 naringenin과 kaempferol에서는 교배친 보다 높은 함량을 보이는 계통을 발견할 수 없었다. 총 플라보노이드 함량에서 kampferol은 79.7 %, quercetin은 17.4 %의 비율을 보였고 naringenin의 경우 2.8 %로 거의 대부분의 계통에서 가장 낮은 함량을 보이고 22개 계통에서는 전혀 발견되지 않았다. 또한 성분간 상관관계를 분석한 결과 quercetin과 kaempferol의 함량간에 유의한 상관관계가 있음을 확인할 수 있었다. 본 실험의 결과는 향후 플라보노이드 생합성 기작 관련 유전자 탐색을 위한 자료로 활용할 예정이다. 또한 고정 계통을 대상으로 다양하고 광범위한 형질 조사를 실시하여 배추의 품종 개발이 가능할 것으로 기대된다.

사과와 꽃사과의 구성 아미노산, 무기질 조성 및 항산화 활성 비교 (Comparison of Amino Acid, Mineral Compositions and Antioxidant Activity of Apple and Crab Apple)

  • 이경행;유광원;배윤정;주가영;김채영
    • 한국식품영양학회지
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    • 제35권5호
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    • pp.353-358
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    • 2022
  • To confirm the applicability of crab apple, the composition and content of amino acids and minerals with 'Fuji' apple were measured. Apple and crab apple were extracted with water, 70% and 100% ethanol, respectively. The contents of polyphenols, flavonoids, and ascorbic acid, antioxidant activities of these extracts were measured. The amino acid composition of apples comprised a total of 17 amino acids. The total amino acid contents of apple and crab apple were 2,050.45 mg/kg and 900.05 mg/kg, respectively. For minerals, the total mineral content of apple and crab apple were 489.14 mg%, 529.77 mg%, respectively. The contents of polyphenols, flavonoids, and ascorbic acid of apple and crab apple extracts were highest in 70% ethanol extracts. The content of polyphenols, the crab apple extract, showed a generally higher content than the apple extract. The content of flavonoids, apple and crab apple extracts revealed no difference between extracts. The content of ascorbic acid, apple extract showed a generally higher content than the crab apple extract, but there was no significant difference between extracts. In the case of DPPH and ABTS radical scavenging abilities, 70% ethanol extract showed the highest antioxidant activity, and crab apple showed higher activity than apple extracts.

마이크로웨이브 에너지를 이용한 레몬그라스로부터 플라보노이드 성분의 추출: CCD-RSM을 이용한 최적화 (Extraction of Total Flavonoids from Lemongrass Using Microwave Energy: Optimization Using CCD-RSM)

  • 유봉호;장현식;이승범
    • 공업화학
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    • 제32권2호
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    • pp.168-173
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    • 2021
  • 본 연구에서는 마이크로웨이브 에너지를 이용하여 항산화성분을 다량 함유하고 있는 레몬그라스로부터 플라보노이드 성분을 추출하였다. 또한 반응표면분석법 중 중심합성계획모델을 이용하여 추출공정을 최적화하였다. 추출공정의 독립변수로는 주정/초순수의 부피비, 마이크로웨이브 조사시간, 마이크로웨이브 조사세기를 설정하였고, 반응치는 추출수율과 플라보노이드 함량을 확인하였다. CCD-RSM 분석 결과 최적조건인 주정/초순수 부피비(56.3 vol.%), 마이크로웨이브 조사시간(6.1 min), 마이크로웨이브 조사세기(574.6 W)에서 추출수율(17.2%)와 플라보노이드 함량(44.7 ㎍ QE/mL dw)의 결과를 얻는 것으로 나타났다. 이때 종합만족도는 D = 0.8562이고, P-value는 추출수율(0.037)과 플라보노이드 함량(0.002)으로 나타났다. 이 조건에서의 실제실험 결과 오차율은 5.0% 이하로 나타나 높은 유의수준의 결과를 얻을 수 있었다.

냉동 동결 건조 머큐베리 분말 첨가 파운드케이크의 항산화 활성과 품질 특성 (Antioxidant Activity and Properties Characteristics of Pound Cakes Prepare using Freeze Dried Maquiberry (Aristotelia chilensis [Mol.]) Powder)

  • 이혜정
    • 한국식품영양학회지
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    • 제27권6호
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    • pp.1067-1077
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    • 2014
  • A study was undertaken to examine the effect of adding freeze dried maquiberry powder on the quality of pound cakes. Freeze dried maquiberry was added to the flour at a ratio of 0.5, 1, 1.5 and 2.5%. The antioxidant activity was estimated by DPPH free radical scavenging activity, the total phenolic compounds content, and flavonoid content in freeze dried maquiberry powder of pounds cakes. Antioxidative activity was highly correlated with the total phenolic and total flavonoids contents of freeze dried maquiberry powder of pound cakes (r=0.9126, p<0.001, r=0.8366, p<0.001, respectively). The quality characteristics of freeze dried maquiberry powder of poundcakes were estimated. The specific volume decreased significantly with increased substitution level of freeze dried maquiberry powder (p<0.01). The lightness significantly decreased with increased freeze-dried maquiberry powder of pound cake crust and crumb (p<0.01, p<0.01). The hardness and adhesiveness increased, while gumminess tended to reduce with increased maquiberry powder. The consumer acceptability score of 0.5~2.5% freeze dried maquiberry powder of pound cakes ranked significantly (p<0.01) higher than those of the other groups in overall preference. These results showed that freeze dried maquiberry powder is a good ingredient to increase consumer acceptability and health.

Comparison of the contents of total polyphenol, total flavonoid, and flavonoid derivatives in unfermented and fermented barley sprouts

  • Neil Patrick Uy;Hak-Dong Lee;Dae Cheol Byun;Sanghyun Lee
    • Journal of Applied Biological Chemistry
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    • 제66권
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    • pp.353-358
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    • 2023
  • Barley (Hordeum vulgare) belongs to the Poaceae family. This study compared the polyphenol and flavonoid levels of unfermented and fermented barley sprouts using spectrophotometric assays. The findings indicated that fermentation greatly boosted the flavonoid content but caused only a slight increase in the polyphenol content. However, this does not imply that fermentation has no effects whatsoever on the polyphenol content of barley sprouts. This was due to the fact that some flavonoids cannot be detected by the wavelength used to calculate the overall polyphenol concentration. Both samples were subjected to high-performance liquid chromatography analysis and detected the flavonoids lutonarin, saponarin, isoorientin, isovitexin, and tricin-all of which have bioactive properties-most notably known for their antioxidant activity. These results augment the ongoing phytochemical profiling research and can possibly valorize the already thriving barley industry.

Evaluation of crude protein, crude oil, total flavonoid, total polyphenol content and DPPH activity in the sprouts from a high oleic acid soybean cultivar

  • Mugisha, James;Asekova, Sovetgul;Kulkarni, Krishnanand P.;Park, Cheol Woo;Lee, Jeong-Dong
    • 농업과학연구
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    • 제43권5호
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    • pp.723-733
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    • 2016
  • Soybeans [Glycine max (L.) Merill] are a rich source of antioxidants and other phytonutrients. Soybean sprouts contain many biologically active secondary metabolites and are rich in polyphenols, flavonoids, and phenolic compounds. In the present study, two soybean cultivars, Hosim, with high oleic acid (- 80% in total seed oil), and Pungsannamul, with normal oleic acid (- 23%) in seed, were examined for changes in the content of crude protein, crude oil, total flavonoids, total phenolics, and DPPH (1,1-diphenyl-2-picryl-hydrazyl) during the sprouting duration of 5 days. The protein content in both the varieties was found to increase by the days of sprouting. The crude oil content of Pungsannamul sprouts was found to be maximum on day 1 (16.9%, w/w) and decreased thereafter to reach to the level of 14.8% on day 5. No significant differences in the crude oil content of Hosim sprouts from day 1 to 5 were observed. Flavonoid content was found to increase up to day 4 and then dropped on day 5, in both the cultivars. Total polyphenol content showed a tendency to increase up to day 3 and started to decrease significantly from day 4. DPPH activity was found to increase up to day 5 in both the varieties. All the components studied in the high oleic acid soybean sprouts showed a change in content during the sprouting process similar to the change that would occur in normal oleic acid soybeans. The study showed that the contents of antioxidant, flavonoid, and polyphenol significantly increase during the sprouting.

고추냉이 잎의 함유성분 분석과 항산화 활성 (Component Analysis and Antioxidant Activity of Wasabi japonica Matsum Leaves)

  • 박성진;이현용
    • 한국약용작물학회지
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    • 제23권3호
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    • pp.207-213
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    • 2015
  • The purpose of this study was to determine the possibility of using Wasabi japonica Matsum leaves as natural health food source. To accomplish this purpose, the contents of general and antioxidative nutrients of Wasabi japonica Matsum leaves were measured. The contents of carbohydrate, crude protein, crude lipid and ash are 53.41%, 25.00%, 7.95% and 13.64%. And the calories of Wasabi leaves was 385.23 Kcal. Total dietary fiber was 52.27%. The K was the largest mineral followed by Ca, P, Mg which means Wasabi leaves is alkali material. The contents of sinigrin and allyisothiocyanate in the wasabi leaves were 69.2 mg/g and 241.0 mg/g, respectively. Total phenol contents of the hot water extract and the 70% ethanolic extract were $19.44{\pm}0.23$ and $19.33{\pm}1.17mg$ GAE/g, respectively. The total flavonoids content of the hot water extract and the 70% ethanol extract were $7.69{\pm}0.71$ and $19.25{\pm}1.41mg$ QE/g, espectively. The general nutrients and other antioxidant bioactive materials in Wasabi japonica Matsum leaves were also potential materials for good health food.

Comparison of Biological Activities of Dendropanax morbiferus by Different Cultivation Areas in Korea

  • Oh, Junseok;Hong, Jae-Heoi;Park, Tae-Young;Jeon, Byeonggyu;Ha, Neul-I;Kim, Kyung Je;Jin, Seong Woo;Koh, Young Woo;Im, Seung Bin;Jeong, Hee Gyeong;Seo, Kyoungsun
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2019년도 춘계학술대회
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    • pp.105-105
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    • 2019
  • Dendropanax morbifera H.Lev is an evergreen tree that lives in subtropical climates. About 75 species of D. morbifera live in East Asia, but only one species live in Korea(Korean dendropanax). D. morbifera belonging to Aralicacae family 98% of D. morbifera are distributed in Jeollanam-do in the Korea and the grow wild in Gyeongsangnam-do and Jeju Island. The harvest time and usage of D. morbifera were recorded in traditional medicinal books. The roots and stems of D. morbifera had been used for traditional medicine to treat migraine, menstrual irregularity and skin disease. And D. morbifera leaves are contain flavonoids and polyacetylene compounds. In this study, we were investigated the physiological activity of D. morbifera by different areas collected at the same time, and compared to characteristics of plants. D. morbifera collected from Jeollanam-do (Goheung-gun), Gyeongsangnam-do (Namhae-gun) and Jeju Island, and dried at $50^{\circ}C$ for three days. We used dried D. morbifera powder for antioxidant tests. Each sample was extracted with hot water under the same conditions. The contents of total polyphenols and total flavonoids from D. morbifera were identified. Also, we performed to DPPH radical scavenging activity, ABTS cation radical scavenging activity and Superoxide anion scavenging activity efficacy for antioxidant activity determination. The contents of total polyphenols and total flavonoids in hot water extract of D. morbifera harvested from Gyeongsangnam-do and Jeollanam-do were higher than Jeju. However, D. morbifera harvested from Gyeongsangnam-do and Jeollanam-do showed no significant difference those content of total polyphenols and total flavonoids. And the antioxidant capacity was showed the similar patterns in antioxidant activity.

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Variation of Nutritional and Antioxidant Characteristics of Extract of Lycium barbarum produced by using Different Extraction Processes

  • Ho-Jong You
    • 한국응용과학기술학회지
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    • 제41권2호
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    • pp.520-529
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    • 2024
  • Lycium barbarum extract has a high potential to be developed as a health functional food due to the various health-promoting effects of Lycium barbarum. This study analyzed changes in nutritional and functional components depending on the extraction solvent (purified water and a mixture of purified water and alcohol) and the condition of the sample. The nutritional components (carbohydrates, protein, fat, ash), organic acids, amino acids, total phenolic compounds, and total flavonoids of the extract produced during the extraction process were analyzed. The nutritional composition and functional substances of the extracts showed some differences depending on the type of solvent and the condition of the sample. The amounts of crude protein (7.61%), crude fat (1.63%), carbohydrate (90.22%), and ash (0.54%) of dried Lycium barbarum extract using purified water as a solvent were similar to those of the powder sample extract. The highest content of citric acid was 4.31 mg/mL, similar to the case of acetic acid, when the powder sample used a mixture of purified water and alcohol as a solvent. The highest amino acid content was 357.39 mg/mL when the powder sample was mixed with purified water and alcohol as a solvent. The total amount of phenolic compounds was 686.16 g/L when the powder sample was extracted with a mixture of purified water and alcohol as a solvent. The highest total flavonoid content was 111.32 g/L when the powder sample was extracted with a mixture of purified water and alcohol as a solvent.