• Korean Journal of Medicinal Crop Science
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• v.15 no.2
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• pp.73-81
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• 2007

An efficient regeneration system was developed using leaf, petiole, and internode explants. Highly embryogenic callus was obtained following cultivation on MS basal nutrient supplemented with 2 $mg/{\ell}$ 2,4-D. Globular, heart, torpedo and cotyledon shaped somatic embryo were produced from the surface of embryogenic callus. Direct shoot regeneration without intermediate callus formation has been achieved on MS medium supplemented NAA and BAP. The percentage of response varies with different concentration of auxin and cytokinin treated individually or in combination. The best shoot regeneration response (54.28%) and number of shoot per explant (12.67) were achieved on the medium supplemented with 0.1 $mg/{\ell}$ NAA and 1 $mg/{\ell}$ BAP. The regenerated shoot transformed into young plant when cultured into elongation and root induction medium. More than 90% of in vitro propagated plants could survive when transferred to the greenhouse for acclimation. This optimized regeneration system can be used for rapid shoot proliferation and genetic transformation.

• Current Research on Agriculture and Life Sciences
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• v.16
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• pp.31-36
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• 1998

In order to investigate the effect of cytokinins on the cotyledonary variability of somatic embryos in Heracleum moellendorffii Hance., somatic embryos were induced from the immature zygotic embryo on MS medium containing 1.0mg/l 2, 4-D, and then transferred to 2,4-D-free and cytokinin-added medium for somatic embryogenesis after 4weeks culture. Polycotyledonary embryos were formed most abundantly(84.9%) on the 0.2mg/l BA medium as compared with the 0.2mg/l 2ip(42.6%) and kinetin(32.9%) media, and it was proportional to BA concentrations(0.01~0.1mg/l). The rate of polycotyledonary embryo formation increased proportional to the period of BA treatment and also increased more at the globular stage than at the heart, torpedo-shaped and cotyledonary stages.

• Journal of the Society of Naval Architects of Korea
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• v.56 no.4
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• pp.298-307
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• 2019

The evolution of the cavity and the variation of the drag for an underwater body with control fins are investigated through a numerical analysis of the steady cavitating turbulent flow. The continuity and the steady-state RANS equations are numerically solved using a mixture fluid model for calculating the multiphase turbulent flow of air, water and vapor together with the SST $k-{\omega}$ turbulence model. The method of volume of fluid is applied by the use of the Sauer's cavitation model. Numerical solutions have been obtained for the cavity flow about an underwater body shaped like the Russian high-speed torpedo, Shkval. Results are presented for the cavity shape and the drag of the body under the influence of the gravity and the free surface. The evolution of the cavity with the body speed is discussed and the calculated cavity shapes are compared with the photographs of the cavity taken from an underwater launch experiment. Also the variation of the drag for a wide range of the body speed is investigated and analyzed in details.