• Tribology and Lubricants
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• 제37권1호
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• pp.31-40
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• 2021

This study describes the effects of a thrust labyrinth seal applied to the backside of a centrifugal impeller on the axial thrust force for high speed turbomachinery. The bulk flow model using Neumann's equation calculates the seal cavity pressures and leakage flow rate of the thrust labyrinth seal based on three configurations: teeth-on-rotor (TOR), teeth-on-stator (TOS), and interlocking labyrinth seal (ILS). Prediction results show that the ILS is superior to the TOR and TOS in terms of leakage flow rate. A mathematical model of a centrifugal impeller with a thrust labyrinth seal on its backside calculates the force components corresponding to the impeller inlet, shroud, impeller backside outer, backside seal, and backside inner pressures. A summation of the force components renders the total axial thrust force acting on the centrifugal impeller. The Newton-Raphson numerical scheme iteratively calculates the pressures and leakage flow rate through the impeller wall gap. The prediction results reveal that the leakage flow rate and total axial thrust force increase with rotor speed, and the ILS significantly decreases the leakage flow rate, whereas it slightly increases the axial thrust force when compared to TOR and TOS. Increasing the seal clearance causes an increase in the leakage flow rate and a slight decrease in the axial thrust force with the ILS.

• 한국식품과학회지
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• 제50권1호
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• pp.105-110
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• 2018

멜리틴은 봉독의 주요 성분 중 하나로 항염증과 항암활성 효과를 가지고 있다. 우리는 폐암세포에서 멜리틴이 EMT 억제를 통해 암세포 이동과 침투를 억제하는 사실을 확인하였다. 멜리틴은 EGF로 유도된 폐암 세포 이동과 침투를 억제하였을 뿐만 아니라 EMT와 관련된 단백질인 이카드헤린의 발현을 증가시켰으며, 바이멘틴과 피브로넥틴 발현은 감소시켰다. 또한 멜리틴에 의한 EMT조절 전사인자인 ZEB2, Slug, Snail의 발현을 확인한 결과 멜리틴 처리에 의해 농도의존적으로 발현이 감소하였다. 또한 작용 메커니즘을 확인하기 위해 mTOR와 FAK 메커니즘을 확인한 실험에서 EGF 처리에 의해 증가한 AKT, mTOR, p70S6K, 4EBP1의 인산화가 멜리틴 농도의존적으로 감소하였다. 그러나 FAK는 EGF에 의해 변화가 없었으며, EKR, JNK 메커니즘은 EGF 처리에 의해 인산화가 증가하였으나 멜리틴 처리에 의해 아무런 영향을 받지 않았다. 그러므로, 폐암세포의 세포 이동과 침투에 대한 멜리틴의 억제효과는 AKT/mTOR/P70S6K/4EBP1 기전 억제를 통해 EMT를 억제하여 세포 이동과 침투를 억제하는 것으로 보인다.

• 생명과학회지
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• 제32권6호
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• pp.421-429
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• 2022

본 연구에서는 죽상경화 플락에서 발견되는 펩티도글리칸이 혈관염증에서 어떠한 역할을 하는지 알아보기 위하여 염증성 사이토카인의 한 종류인 인터루킨-1 알파의 발현에 대한 영향을 조사하였다. 실험방법으로는 혈관염증을 주도하는 단핵구/대식세포인 THP-1 세포주에 펩티도글리칸을 처리하고 인터루킨-1 알파의 발현을 RT-PCR, real-time PCR, ELISA 방법으로 분석하였다. 펩티도글리칸의 처리 시간과 농도에 비례하여 단핵구/대식세포에서 인터루킨-1 알파의 전사체와 단백질 분비가 증가함을 관찰하였다. 또한 펩티도글리칸의 작용기전을 규명하기 위하여 신호전달을 차단하는 억제제를 세포에 처리하고 인터루킨-1 알파의 발현을 조사하였다. TLR2/4의 억제제인 OxPAPC 그리고 세포 kinase의 작용을 억제하는 LY294002(PI3 kinase 억제), Akti IV (Akt 억제), rapamycin (mTOR 억제), U0126 (MEK 억제), SB202190 (p38 MAPK 억제), SP6001250 (JNK 억제), DPI (NOX 억제)를 처리하는 경우 인터루킨-1 알파 전사체의 발현 그리고 단백질의 분비가 감소되었다. 반면에 LPS의 작용을 억제하는 polymyxin B는 인터루킨-1 알파의 발현에 영향을 주지 않았다. 이상의 결과는, 펩티도글리칸이 TLR2, PI3K, Akt, mTOR, MAPKs를 통하여 단핵구/대식세포의 인터루킨-1 알파 발현을 증가시키고 혈관염증에 기여한다는 것을 나타낸다.

• 대한치주과학회:학술대회논문집
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• 대한치주과학회 2005년도 제45회 종합학술대회 연제초록
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• pp.103-104
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• 2005

• 대한수학회논문집
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• 제1권1호
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• pp.91-97
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• 1986

• Archives of Plastic Surgery
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• 제48권3호
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• pp.245-245
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• 2021

• BMB Reports
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• 제49권2호
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• pp.71-72
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• 2016

Focal cortical dysplasia type II (FCDII) is a focal malformation of the developing cerebral cortex and the major cause of intractable epilepsy. However, since the molecular genetic etiology of FCD has remained enigmatic, the effective therapeutic target for this condition has remained poorly understood. Our recent study on FCD utilizing various deep sequencing platforms identified somatic mutations in MTOR (existing as low as 1% allelic frequency) only in the affected brain tissues. We observed that these mutations induced hyperactivation of the mTOR kinase. In addition, focal cortical expression of mutant MTOR using in utero electroporation in mice, recapitulated the neuropathological features of FCDII, such as migration defect, cytomegalic neuron and spontaneous seizures. Furthermore, seizures and dysmorphic neurons were rescued by the administration of mTOR inhibitor, rapamycin. This study provides the first evidence that brain somatic activating mutations in MTOR cause FCD, and suggests the potential drug target for intractable epilepsy in FCD patients.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3419-3423
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• 2013

Of all gynecologic cancers, endometrial cancer is the most common cancer in the US and Europe. In addition, it is presently the second most common gynecologic cancer in the world. As a result of increasing menopausal, obese and tamoxifen use women, the incidence of the cancer seems to be on the increase. Surgery is the major treatment, whereas postoperative radiation therapy in high-intermediate risk patients many prevent locoregional recurrence. Adjuvant chemotherapy can improve progression free survival in advanced or recurrent cancers. Molecular targeted therapies are now a focus of attention including anti-vascular endothelial growth factor (VEGF), mammalian target of rapamycin (mTOR) inhibitor and tyrosine kinase inhibitor (TKI). They may provide useful future strategies for control of endometrial malignancies in developing countries and across the world.

• Interaction and multiscale mechanics
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• 제1권4호
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• pp.449-465
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• 2008

In this paper a general approach for studying the motion of a cantilever beam interacting with a 2D fluid flow is presented. The fluid is solved by a general purpose commercial computational fluid dynamics (CFD) package (FLUENT 6.2), while the structure is managed by means of a dedicated finite element method solver, coded in FLUENT as a user-defined function (UDF). A weak fluid structure interaction coupling scheme is adopted exchanging information at the end of each time step. An arbitrary cantilever beam can be introduced in the CFD mesh with its wetted boundaries specified; the cantilever can also interact with specified rigid and flexible walls through use of a non-linear contact algorithm. After a brief review of relevant scientific contributions, some test cases and application examples are presented.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2005년도 추계학술대회 논문집 전력기술부문
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• pp.35-38
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• 2005

This paper suggests the method that forecast Dynamic Line Rating (DLR). Thermal Overload Risk (TOR) of next time is forecasted based on current weather condition and DLR value by Monte Carlo Simulation (MCS). To model weather element of transmission line for MCS, we will propose the use of weather forecast system and statistical models that time series law is applied. Also, through case study, forecasted TOR probability confirmed can utilize by standard that decide DLR of next time. In short, proposed method may be used usefully to keep safety of transmission line and reliability of supply of electric Power by forecasting transmission capacity of next time.