• Journal of the Korean Magnetic Resonance Society
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• v.12 no.1
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• pp.1-13
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• 2008

Acetyl-CoA carboxylase (ACC) catalyzes the first step in fatty acid biosynthesis: the synthesis of malonyl-CoA from acetyl-CoA. As essential regulators of fatty acid biosynthesis and metabolism, ACCs are regarded as therapeutic targets for the treatment of metabolic diseases such as obesity, In ACC, the biotinoyl domain performs a critical function by transferring an activated carboxyl group from the biotin carboxylase domain to the carboxyl transferase domain, followed by carboxyl transfer to malonyl-CoA. Despite the intensive research on this enzyme, only the bacterial and yeast ACC structures are currently available, To explore the mechanism of ACC holoenzyme function, we determined the structure of the biotinoyl domain of human ACC2 and analyze its characteristics using NMR spectroscopy. The 3D structure of the hACC2 biotinoyl domain has a similar folding topology to the previously determined domains from E. coli and P. Shermanii, however, the 'thumb' structure is absent in the hACC2 biotinoyl domain. Observations of the NMR signals upon the biotinylation indicate that the biotin group of hACC2 does not affect the structure of the biotinoyl domain, while the biotin group for E. coli ACC interacts directly with the thumb residues that are not present in the hACC2 structure. These results imply that, in the E. coli ACC reaction, the biotin moiety carrying the carboxyl group from BC to CT can pause at the thumb of the BCCP domain. The human biotinoyl domain, however, lacks the thumb structure and does not have additional non-covalent interactions with the biotin moiety; thus, the flexible motion of the biotinylated lysine residue must underlie the "swinging arm" motion. This study provides insight into the mechanism of ACC holoenzyme function and supports the "swinging arm" model in human ACCs.

• Journal of Internet Computing and Services
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• v.22 no.6
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• pp.115-127
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• 2021

With the development of science and technology around the world, the realm of cyberspace, following land, sea, air, and space, is also recognized as a battlefield area. Accordingly, it is necessary to design and establish various elements such as definitions, systems, procedures, and plans for not only physical operations in land, sea, air, and space but also cyber operations in cyberspace. In this research, the importance of cyber targets that can be considered when prioritizing the list of cyber targets selected through intermediate target development in the target development and prioritization stage of targeting processing of cyber operations was selected as a factor to be considered. We propose a method to calculate the score for the cyber target and use it as a part of the cyber target prioritization score. Accordingly, in the cyber target prioritization process, the cyber target importance category is set, and the cyber target importance concept and reference item are derived. We propose a TIR (Target Importance Rank) algorithm that synthesizes parameters such as Event Prioritization Framework based on PageRank algorithm for score calculation and synthesis for each derived standard item. And, by constructing the Stuxnet case-based network topology and scenario data, a cyber target importance score is derived with the proposed algorithm, and the cyber target is prioritized to verify the proposed algorithm.