• Weed & Turfgrass Science
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• v.5 no.1
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• pp.29-34
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• 2016

Large patch disease in Zoysia japonica Steud. is the most destructive disease in turfgrass. For large patch management, it has been dependent on chemical controls but pesticides are harmful to soil, water and biodiversity. In this study, we evaluated 4 Streptomyces spp. strains (S2, S5, S8 and S12) which were selected in previous studies using metagenome approaches. Root colonization of the strains, large patch suppressing effect and the pathogen density change in actual golf course were investigated to evaluate biological control potential of the strains. All strains exhibited reliable root colonization ability that strains populations were higher than $6log\;cfu\;g^{-1}$ in turfgrass rhizosphere. The pathogen density, with S8 treatment, was detected average of 0.7 after a week and average of 1.2 after 4 weeks. Disease control and suppressive the pathogen population by S8 strain showed higher efficiency than other strains. S8 was applied in an actual golf course for the large patch control and pathogen density. The pathogen density in S8 treatment plot was detected below 1.6 per toothpick and lower compared with untreated plot. The results indicated that pathogen density was suppressed by S8 and the stain has great potential as a biological control agent for the large patch.

• Journal of Microbiology
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• v.34 no.4
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• pp.327-333
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• 1996

Yeast, like many other microbes, encounters large variations in ambient pH in their natural environments. Microorganisms capable of growing over a wide pH range require a versatile, efficient pH homeostatic mechanism protecting intracellular processes against extremes of pH. In several organisms, fusions to the bacterial lacZ gene have been extremely useful for the identification of genes expressed at different time during the life cycle or under different growth conditions. In this study, using the lacZ gene screening system, we surveyed a large number of yeast strains with lacZ insertion to identify genes regulated by pH. A yeast genomic library was constructed and inserted with lacZ by a shuttle mutagenesis procedure. The yeast transformants were individually picked up with a toothpick, replica-plated, and grown in alkaline pH medium. Among the 35,000 colonies screened, 10 candidate strains were identified initially by the $\beta$-gal assay. We finally confirmed two yeast strains carrying the genes whose expression are strictly dependent on pH of growth medium. One of the fusions showing a 10-fold induction in expression level in response to alkali pH was selected and further characterized. The pH-regulated gene was cloned by inverse PCR and a partial sequence of the gene was determined. Identification and characterization of the gene is currently under investigation.

• Journal of Periodontal and Implant Science
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• v.50 no.2
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• pp.83-96
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• 2020

Purpose: The present study aimed to evaluate the clinical benefit of additional toothbrushing accompanying non-surgical periodontal treatment on oral and general health in patients with type 2 diabetes mellitus (T2DM). Methods: We conducted a doubled-blind randomized controlled trial in 60 T2DM patients between June 2013 and June 2014. The patients were randomly assigned to the scaling and root planing (SRP) group; the scaling and root planing with additional toothbrushing (SRPAT) group, in which additional toothbrushing was performed by toothpick methods; or the control group. Microbiological and oral examinations were performed for up to 12 weeks following treatment. Non-surgical treatment was conducted in the experimental groups. The SRP group received scaling and root planing and the SRPAT group received additional toothbrushing with the Watanabe method once a week from the first visit through the fifth visit. The primary outcomes were changes in haemoglobin A1c (or glycated haemoglobin; HbA1c) levels, serum endotoxin levels, and interleukin-1 beta levels. Periodontal health status was measured by periodontal pocket depth, the calculus index, and bleeding on probing (BOP). Results: Both the SRP and SRPAT groups showed improvements in periodontal health and HbA1c, but the SRPAT group showed significantly less BOP than the SRP group. Furthermore, only the SRPAT group showed a statistically significant decrease in serum endotoxin levels. Conclusions: Non-surgical periodontal treatment was effective in improving HbA1c and serum endotoxin levels in T2DM patients. Furthermore, non-surgical treatment with additional tooth brushing had a more favourable effect on gingival bleeding management. Trial RegistrationClinical Research Information Service Identifier: KCT000416.

• Journal of Korean society of Dental Hygiene
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• v.9 no.4
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• pp.580-592
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• 2009

Objectives : This study had been performed for Respondents who live in Daegu and Kyungbuk province Age group of 30 to 50 years old. Methods : The oral health state and oral heath care, dental treatments about the use of oral hygiene devices were obtained through self-administering questionnaires from 2 to 31, January, 2009. Results : 1. The toothpick was well known for respondents and automatic brush, gargle, dental floss were practically used. 2. 57.1% of man know oral hygiene device, they know more about oral hygiene device if they have higher degree, and they use more if they are older than others. 3. 55.5% of respondents who think their oral health condition is not healthy enough recognize oral hygiene device, 77.5% of respondents who visited dental clinic around six month recognize oral hygiene device. 82.4% of respondents who had been follow-up. 86.1% of respondents who think their oral health state is good enough doesn't use oral hygiene device, oral hygiene device was used more for respondents who visited dental clinic frequently. 47.1% of respondents who visited dental clinic periodically use oral hygiene device. 4. Respondents who experience implant recognize more about oral hygiene device and periodontal Tx, orthodontics problem, preservative treatment were next. 5. 60.0% of respondents who were educated tooth brushing method recognize it. Oral hygiene device was frequently used if tooth brushing time were increased. 26.4% of respondents who were changed their tooth brushing method used oral hygiene device. Conclusions : Their recognition level was practically low whose age is around 30 to 50. Their oral hygiene device use ratio is higher then others who were educated tooth brush method so that I think we need to recommend for use oral hygiene device and use method.

• Hwankyungkyoyuk
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• v.11 no.1
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• pp.151-164
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• 1998

Conclusion and the improvement plan according to the survey on recycling wastes in 'the program of woman and environmental education' mainly for the chairwomen of the women's association of the apartment house in City and County of Chungchongnamdo during the first half of 1998 (from March tp April) are as follows. 1. Conclusion 1) It showed that the rate of recognition for recycle has no difference by regional groups and the rate of recognition is 57.4% but they mainly know vaguely or they do not know. 2) It showed that the rate they do not know how the separated wastes are recycled is 30.4% and the scope of their knowledge is approximative.(52.6%) 3) It showed that it is the housewives who mainly do the separate garbage collection (72.7%) and 19.1% of the people have no interest in the separate discharge. 4) It showed that the rate they filter the garbage or remove water from the garbage at house is 53.1%. And 20% of the people in the urban region dump untreated wastes but 8.5% of the people in the rural region do the same, so the rate of using garbage in the rural region is higher than that in the urban region. 5) It showed that the separate state of the garbage is 29.2% for the removal of toothpick and paper and 47.4% for the removal of vinyl and stopper. 6) It showed that 66.7% of the motive for recycling waste is the education activity for environment by the women's association and SAEMAEUL association and 34.5% of that is the influence of TV and radio. 7) It showed that the rate of making compost and feed using garbage in the rural region is higher than that in the urban region and in some urban regions, the rate they sprinkle the garbage in provisional compost state on the floor garden is high. 8) It showed that the recognition rate for the material of separately collected garbage corresponding to the separate waste system of 5-6 classification is 12.5% 9) It showed that the major variable which has an effect on the recycle is the education activity for environment by the neighborhood meeting(P<0.05) and by the women's association of saemaeul activity(P<0.05)

• Journal of dental hygiene science
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• v.19 no.3
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• pp.162-169
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• 2019

Background: Oral diseases are caused by various systemic and local factors, the most closely related being the biofilm. However, the challenges involved in removing an established biofilm necessitate professional care for its removal. This study aimed to evaluate and compare the effects of professional self and professional biofilm care in healthy patients to prevent the development of periodontal diseases. Methods: Thirty-seven patients who visited the dental clinic between September 2018 and February 2019 were included in this study. Self-biofilm care was performed by routine tooth brushing and professional biofilm care was provided using the toothpick method (TPM) or the oral prophylaxis (OP) method using a rubber cup. Subgingival bacterial motility and halitosis (levels of hydrogen sulfide, $H_2S$; methyl mercaptan, $CH_3SH$; and di-methyl sulfide, $(CH_3)_2S$) were measured before, immediately after, and 5 hours after the preventive treatment in the three groups. Repeated measures analysis of variance test was performed to determine significant differences among the groups. Results: TPM was effective immediately after the prevention treatment, whereas OP was more effective after 5 hours (proximal surfaces, F=16.353, p<0.001; smooth surfaces, F=66.575, p<0.001). The three components responsible for halitosis were effectively reduced by professional biofilm care immediately after the preventive treatment; however, self-biofilm care was more effective after 5 hours ($H_2S$, F=3.564, p=0.011; $CH_3SH$, F=6.657, p<0.001; $(CH_3)_2S$, F=21.135, p<0.001). Conclusion: To prevent oral diseases, it is critical to monitor the biofilm. The dental hygienist should check the oral hygiene status and the ability of the patient to administer oral care. Professional biofilm care should be provided by assessing and treating each surface of the tooth. We hope to strengthen our professional in biofilm care through continuous clinical research.

• Weed & Turfgrass Science
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• v.4 no.4
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• pp.376-382
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• 2015

Dollar spot, caused by Sclerotinia homoeocarpa, is the major disease in cool-season turfgrasses. Understanding the distribution of this pathogen in soil and thatch is important to developing disease control strategies. In this study, toothpicks were used to detect S. homoeocarpa in the turfgrass canopy, thatch, and soil at different distances from dollar spot infection centers. The effect of penetrant and contact fungicide applications with different water volumes on distribution of S. homoeocarpa was also investigated. S. homoeocarpa was detected in 100% of samples taken from the leaf canopy, 83.3% in thatch area, and 0% in the soil from within the infection center. S. homoeocarpa was isolated in 100% of samples taken from the edge of the infection center, but was only detected in 13% of the samples taken at 1.5 cm away from the infection center edge. S. homoeocarpa was isolated at a higher frequency in the propiconazole treated plots than those treated with chlorothalonil and was not detected in leaf canopy samples when either fungicides was applied with 6.78 L of water. In conclusion, the toothpick-aided detection technique has improved our understanding of S. homoeocarpa epidemiology and could be used as a diagnostic tool to detect for fungicide resistance on golf courses.

• Korean Journal of Agricultural and Forest Meteorology
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• v.5 no.4
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• pp.213-217
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• 2003

Forest fire spread and intensity were modeled as a function of wind and fuel. Spread rate and intensity of forest fire were related to weight and thickness of forest fuel beds and to wind speed. Forest fire spread rate and fire intensity were differentiated according to wind speed. Rapid wind speed causes a faster forest fire spread rate and greater fire intensity than does slow wind speed. Relative burning time of the fire from beginning to end in the model was 161 sec at a wind speed of 0.5 m/sec and 146 sec at 1m/sec on the model. Average forest lire spread rate was 0.014 m/sec at a wind speed of 0.5 m/sec and 0.020 m/sec at 1m/sec. Average fire intensity was 0.183 ㎾/m at a wind speed of 0.5 m/sec, 0.259 ㎾/m at 1m/sec. Fire intensity was greater when forest fire spread rate was rapid.

• Journal of dental hygiene science
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• v.15 no.2
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• pp.220-225
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• 2015

This study was conducted to examine O'leary index, patient hygiene performance (PHP) index, and toothbrushing practice assessment in subjects of college students in 20s who had been randomly selected. The purpose of this study is to examine if it is easy for rolling method which is recommended by many dental professionals in Korea to implement and to effectively remove dental plaque. Also, the correlations between dental plaque index and toothbrushing practice assessment with the course of time was confirmed, after instruction session on toothbrushing was provided. STATA 11.0 (StataCorp) was used for analysis. There was no significant difference on the three ways of O'leary index, PHP index, and toothbrushing practice assessment in using rolling method, bass technique and toothpick method when comparing the average resulting from first to third instruction session on toothbrushing. O'leary index, PHP index, and toothbrushing practice assessment were inspected with Kruskal-Wallis test which is used for non-parametric statistics. They were checked three times: the first, before the toothbrushing instruction was given; the second, two weeks after the toothbrushing session was given; and the third, 4 weeks after the session. The results are as follows: O'leary index stood at the lowest in the first experiment but showed the highest in the second (p=0.0001). PHP index was the highest level in the first trial and decreased in the second time, but increased again in the last examination (p=0.0001). Toothbrushing practice assessment also showed a similar tendency with PHP index (p=0.0001). In conclusion, rolling method is not the best option for everyone, and it is thought that more various toothbrushing ways need to be reviewed and recommended to people. Also, institutional framework is required for the continuous education on toothbrushing to be in place as the effectiveness of the education is decreased with time.

• Journal of Periodontal and Implant Science
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• v.34 no.3
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• pp.563-572
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• 2004

Since bacterial plaque is known as the major cause of every oral disease, it is necessary to control plaque to maintain periodontal health. Although tooth brushing has been the typical method of plaque control, additional use of plaque control devices are necessary for cleansing interdental areas. Dental floss has been used for a long time as the additional plaque control device and proved to be effective. The newly developed embossed new dental pick is composed of elastic plastic material in form with its tip longer than other toothpick. In addition, as thickness of the tip gradually increases, it can be adapted easily with minimal trauma to interdental gingiva. The embossed tip can remove dental plaque easily, and the opposite end can approach lingual and palatal areas through triple angulation. Therefore, the authors studied the effect of embossed new dental pick and dental floss on periodontally involved 15 patient. The following results were obtained. 1. For embossed new dental pick, probing depth at baseline, 1 week, 4 weeks were $3.6{\pm}0.6mm$, $3.4{\pm}0.5mm$, $3.2{\pm}0.5mm$, respectively, and $4.2{\pm}0.9mm$, $4.1{\pm}0.9mm$, $4.0{\pm}0.9mm$, respectivly for clinical attachment level. 2. In case of embossed new dental pick, bleeding on probing at baseline, 1 week, 4 weeks were $43.0{\pm}4.4%$, $28.5{\pm}5.1%$, $22.2{\pm}5.6%$, respectively, and, at 1 week and 4 weeks, they were significantly different from that of dental floss. 3. Gingival index of toαhpick at baseline, 1 week, 4 weeks were $1.7{\pm}0.5$, $1.0{\pm}0.3$, $0.9{\pm}0.2$, respectively, and, $1.7{\pm}0.5$, $1.1{\pm}0.4$, $1.0{\pm}0.4$, respectively for plaque index, and $3.0{\pm}0.5$, $1.7{\pm}0.5$, $1.4{\pm}0.4$, respectively for proximal plaque index. proximal plaque index at 4 weeks was significantly different from that of dental floss. 4. The result of questionnaire showed that the two types of device were both satisfactory, however, embossed new dental pick had higher preference as well as satisfaction. As a result, embossed new dental pick is thought to be clinically effective for its simplicity and good accessibility.