Transgenic animals have been widely used for developmental biology studies, as disease models, and even in industry such as transgenic bioreactor animals. For transgenic birds, quail has the great advantages of small body size, short generation time, and frequent egg production. To date, retroviral or lentiviral transduction has been used to generate transgenic quail for various purposes. However, the efficiency of transgenic offspring production with these methods is relatively low and viral vector usage has safety issues. Unfortunately, non-viral transgenesis has not been established in quail due to a deficiency of stem cell and germ cell culture systems. In this study, we established a direct in ovo lipofection method that could be used to create transgenic quail without germline-competent cells or viruses. To optimize the injection stage during embryo development, the liposome complex (containing piggyBacCMV-GFP and transposase plasmids) was introduced into an embryonic blood vessel at 50 hr, 55 hr or 60 hr. GFP expression was detected in various tissues (heart, kidney, liver and stomach) on day 12 of incubation under a fluorescence microscope. Additionally, GFP-positive cells were detected in the recipient embryonic gonads. In conclusion, the direct in ovo lipofection method with the piggyBac transposon could be an efficient and useful tool for generating transgenic quail.
Purpose: The purpose of this study was the examination of the correlations between the daily physical pain intensity (DPPI) scale and the numeric rating scale (NRS), and between the DPPI scale and the quality of life (QOL) of short form health survey (SF-36) and beck depression inventory (BDI) questionnaires. The focus of the DPPI scale was the daily-living activities of the individual, and the scale contains three items regarding the pain per movement range, the pain per day, and the pain when touching the pain area. Methods: A total of 241 adults answered the DPPI, NRS, SF-36, and BDI questionnaires. Pearson's correlation coefficients were calculated for the various relations of the DPPI to the other scales. Results: High correlations were shown between the NRS and the DPPI (r=0.809, p<0.05). The DPPI scale (r=0.437, p<0.05) showed "moderate" significant correlations with the SF-36 and the NRS (r=0.370, p<0.05), and it showed "weak" significant correlations with the SF-36. There are no statistically significant correlations between the DPPI, the NRS score, and the BDI score (p>0.05). Conclusion: This study was the first attempt to establish the concurrent validity of a new focus on daily-living activities for the assessment of pain. This study showed promise for the development of activities of daily living focused tool for an assessment of the subjective pain in patients that was more objective.
Radiation technology (RT) has long been applied in various fields for increasing the safety and shelf-life of foods by controlling pathogen-induced poisoning. RT was introduced for the first time in Korea in the 1950s to eliminate harmful microorganisms in food materials. In the 1980s, RT had been scientifically proven to be effective for the sterilization of food and public health products. In recent years, irradiation with gamma rays has also been used for improving physiological properties through the structural modification of natural molecules, which has been proposed to be applicable to various industries. In particular, radiation transformation technology (RTT), which involves the development of new functional compounds through the molecular conversion of natural biomaterials, is becoming a new high-value technology as a fusion technique of RT and biotechnology. The present reports have suggested that RTT can be an effective tool for the development of new functional compounds and improvement of the physiological activity of biomolecules.
The purpose of this study was to investigate the status of current nutrition labeling on the packaging of the processed foods that provide consumers with a reliable and consistent source of information, which has been considered as a useful aid for food selection and a potent educational tool for nutrition in daily life. The 2,160 processed foods purchased at the supermarket on September, 2002, were divided by food category issued from the 2002 food codes and assessed in the terms of the nutrition composition labeling and nutrition claims. Nutrition composition labeling was found on 356 of the 2160 processed foods items. Milk and dairy products had 49.7% of nutrition composition labeling, which was the largest number among the food category. Tables were most frequently used as the type of nutrition composition labeling (79.8%). Nutrition composition including many different ways of expression, such as a table of nutrition composition, indication of nutrition composition, analysis table of nutrition composition and comparative table of nutrition composition, made frequent use of nutrition composition labeling titles (78.7%). The various unit of measures were use in the nutrition labeling of the processed foods, per l00g or 100$m\ell$ was the highest (44.6%) under the currently practiced nutrition labeling. The correct labeling standard with nutrient content and % RDA except energy, was used on 47.8% of labels, and those with only liability indication nutrient and liability indication nutrients plus discretion indication nutrients were 25.3 and 22.5% respectively. The processed foods with nutrition claims were 8.0% (172 items). Nutrition claims were divided in two ways: nutrient content claims and nutrient comparative claims. The most frequently used claims were contained in the former (44.4%) and more or plus in the latter case (16.3%). Ca was the most popular item as a nutrition claim nutrient (50.6%).
Journal of the Korea Academia-Industrial cooperation Society
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v.15
no.5
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pp.3203-3212
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2014
Company. s support for culture and arts started from mecenat activities. After then, it played a role as a tool of the company to satisfy consumers. cultural desires and improve the company. s image. As time goes by, it became a culture marketing which encourages business activities. Therefore, many companies try to develop their culture marketing activities in various fields. Especially art marketing has increased consumers. expectation and interest about art activities. Also, art marketing has become important as it has helped consumers to have critical view about products and improve their standard of life. As a way of art marketing, a company provides diverse exhibitions, sponsors and education programs related to art in either a specific space of the company building or a separate place. It can be said that this example of art marketing is a kind of an image marketing and public welfare at the same time. This article presents the concept of culture marketing and current condition of company. s support for culture and arts. In addition, the article depicts the concept and classification of art marketing. Furthermore, through analysis of company exhibition space and programs, the article illustrates the features and typology of art galleries managed by companies.
A diet habit, which is developed in childhood, lasts for a life time. In this sense, nutrition education and early exposure to healthy menus in childhood is important. Children these days have easy access to the internet. Thus, a web-based nutrition education program for children is an effective tool for nutrition education of children. This site provides the material of the nutrition education for children with characters which are personified nutrients. The 151 menus are stored in the site together with video script of the cooking process. The menus are classified by the criteria based on age, menu type and the ethnic origin of the menu. The site provides a search function. There are three kinds of search conditions which are key words, menu type and "between" expression of nutrients such as calorie and other nutrients. The site is developed with the operating system Windows 2003 Server, the web server ZEUS 5, development language JSP, and database management system Oracle 10 g.
Journal of Korean Academy of Fundamentals of Nursing
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v.5
no.2
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pp.257-268
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1998
The purpose of this study were to identify the characteristics of urinary incontinence in adult women and to identify it's relating factors, daily living and sexual problem related urinary incontinence. Subjects were 60 community dwelling women in the age of 25-60 years old at 1 cities in Korea. The data were collected from January 1988 to May. 1998. Subject were interviewed with structured questionnaire. Questionaire was composed of item of general characteristics, obstetrical characteristics, measuring tool of severity of urinary incontinence, discomfort due to urinary incontinence. The data were analyzed with SAS program, descriptive statistics, T-test, ANOVA test. The results of study were as followings. 1) Most of incontinence women were mildly incontinent subjects(mean=7.40). 2) The common activity related to the urinary incontinence were coughing, sneezing, laughing aloudly exercising. 63.5% of women reported small volume accidents of only 1 to 2 drops. 3) The incidence of urinary incontinence was significantly higher in woman who had more pariety and older age of last delivery, menopause, itching sense of vagina. 4) 73.3% of the episodic urinary incontinence were not treated because the felt that urinary incontinence was not disease(70.4%) was shamful(18.1%), was incurable inspite of treatement attempt(4.5%). 5) Daily living problem related to urinary incontinence were as follows : Voiding before going out(66.6%), odor of urine(10.0), frequent underwear change.(88.3%). 6) Sexual intercourse problem related to urinary incontinence were as follows : Pain during sexual intercourse(30.0%), sexual life trouble,(8.3%), urine leakage.(11.7%) The results indicate that urinary incontinence is common in adult women. Health care provider should develop and provide adequate nursing intervention for prevention and early treatment of urinary incontinence.
Complexity paradigm is a scientific amalgam that aims to unite a range of theoretical perspectives and research agendas across natural and social sciences. Proponents of complexity paradigm lay claims to an increasing number of areas of study, including artificial life, interpersonal networks, internal/international patterning of organizations, mapping of cyberspace, etc. All of those can be subsumed under the title, 'complexity turn.' Owing to the idea of open system, complexity paradigm has developed a number of new concepts/themes/perspectives that help to account for the complex mechanism of living and non-living creatures. A complex system comprises a number of properties such as disequilibrium, nonlinearity, dissipative structure, self-organization fractal geometry, autopoiesis, coevolution. Following a brief introduction to theoretical development, those properties are succinctly discussed. The complexity turn has provided a wealth of insights that enable to analyze system operations of any kind. It contributes a lot to illuminating the working of social system as well. The most remarkable attempt may be Niklas Luhmann's 'neofunctional system theory.' Merits and shortcomings of complexity paradigm were examined and its future prospect were assessed with the conclusion that complexity paradigm would continue to be useful both as effective transdisciplinary framework and powerful analytical tool.
Proceedings of the Korea Water Resources Association Conference
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2018.05a
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pp.158-158
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2018
Drought is a recurrent natural hazard in Bangladesh. It has significant impacts on agriculture, environment, and society. Well-timed information on the onset, extent, intensity, duration, and impacts of drought can mitigate the potential drought-related losses. Thus, drought characteristics need to be explained in terms of frequency, severity, and duration. This paper aims to characterize the spatial and temporal pattern of meteorological drought using EDI and illustrated drought severity over Bangladesh. Twenty-seven (27) station-based daily rainfall data for the study period of 1981-2015 were used to calculate the EDI values over Bangladesh. The evaluation of EDI is conducted for 4 sub-regions over the country to confirm the historical drought record-developed at the regional scale. The finding shows that on average, the frequency of severe to extreme drought is approximately 0.7 events per year. As a result of the regional analysis, most of the recorded historical drought events were successfully detected during the study period. Additionally, the seasonal analysis showed that the extreme droughts were frequently hit in northwestern, middle portion of the eastern and small portion of central parts of Bangladesh during the Kharif(wet) and Rabi(dry) seasons. The severe drought was affected recurrently in the central and northern regions of the country during all cropping seasons. The study also points out that the northern, south-western and central regions in Bangladesh are comparatively vulnerable to both extreme and severe drought event. The study showed that EDI would be a useful tool to identify the drought-prone area and time and potentially applicable to the climate change-induced drought evolution monitoring at regional to the national level in Bangladesh. The outcome of the present study can be used in taking anticipatory strategies to mitigate the drought damages on agricultural production as well as human sufferings in drought-prone areas of Bangladesh.
Kim, Dong-Hyeon;Jang, Taeil;Hwang, Syewoon;Cho, Jaepil
Journal of The Korean Society of Agricultural Engineers
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v.61
no.6
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pp.81-92
/
2019
The objective of this study is to evaluate hydrologic impacts of climate change according to downscaling methods using the Soil and Water Assessment Tool (SWAT) model at watershed scale. We used the APCC Integrated Modeling Solution (AIMS) for assessing various General Circulation Models (GCMs) and downscaling methods. AIMS provides three downscaling methods: 1) BCSA (Bias-Correction & Stochastic Analogue), 2) Simple Quantile Mapping (SQM), 3) SDQDM (Spatial Disaggregation and Quantile Delta Mapping). To assess future hydrologic responses of climate change, we adopted three GCMs: CESM1-BGC for flood, MIROC-ESM for drought, and HadGEM2-AO for Korea Meteorological Administration (KMA) national standard scenario. Combined nine climate change scenarios were assessed by Expert Team on Climate Change Detection and Indices (ETCCDI). SWAT model was established at the Mankyung watershed and the applicability assessment was completed by performing calibration and validation from 2008 to 2017. Historical reproducibility results from BCSA, SQM, SDQDM of three GCMs show different patterns on annual precipitation, maximum temperature, and four selected ETCCDI. BCSA and SQM showed high historical reproducibility compared with the observed data, however SDQDM was underestimated, possibly due to the uncertainty of future climate data. Future hydrologic responses presented greater variability in SQM and relatively less variability in BCSA and SDQDM. This study implies that reasonable selection of GCMs and downscaling methods considering research objective is important and necessary to minimize uncertainty of climate change scenarios.
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