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Creation of Consistent Burn Wounds: A Rat Model

  • Cai, Elijah Zhengyang;Ang, Chuan Han;Raju, Ashvin;Tan, Kong Bing;Hing, Eileen Chor Hoong;Loo, Yihua;Wong, Yong Chiat;Lee, Hanjing;Lim, Jane;Moochhala, Shabbir M.;Hauser, Charlotte A.E.;Lim, Thiam Chye
    • Archives of Plastic Surgery
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    • v.41 no.4
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    • pp.317-324
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    • 2014
  • Background Burn infliction techniques are poorly described in rat models. An accurate study can only be achieved with wounds that are uniform in size and depth. We describe a simple reproducible method for creating consistent burn wounds in rats. Methods Ten male Sprague-Dawley rats were anesthetized and dorsum shaved. A 100 g cylindrical stainless-steel rod (1 cm diameter) was heated to $100^{\circ}C$ in boiling water. Temperature was monitored using a thermocouple. We performed two consecutive toe-pinch tests on different limbs to assess the depth of sedation. Burn infliction was limited to the loin. The skin was pulled upwards, away from the underlying viscera, creating a flat surface. The rod rested on its own weight for 5, 10, and 20 seconds at three different sites on each rat. Wounds were evaluated for size, morphology and depth. Results Average wound size was $0.9957cm^2$ (standard deviation [SD] 0.1845) (n=30). Wounds created with duration of 5 seconds were pale, with an indistinct margin of erythema. Wounds of 10 and 20 seconds were well-defined, uniformly brown with a rim of erythema. Average depths of tissue damage were 1.30 mm (SD 0.424), 2.35 mm (SD 0.071), and 2.60 mm (SD 0.283) for duration of 5, 10, 20 seconds respectively. Burn duration of 5 seconds resulted in full-thickness damage. Burn duration of 10 seconds and 20 seconds resulted in full-thickness damage, involving subjacent skeletal muscle. Conclusions This is a simple reproducible method for creating burn wounds consistent in size and depth in a rat burn model.

Introduction of Hindfoot Coronal Alignment View (후족부 관상면 배열 영상에 대한 고안)

  • Moon, Il-Bong;Jeon, Ju-Seob;Yoon, Kang-Cheol;Choi, Nam-Kil;Kim, Seung-Kook
    • Journal of radiological science and technology
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    • v.29 no.4
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    • pp.225-228
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    • 2006
  • Purpose: Accurate clinical evaluation of the alignment of the calcaneus relative to the tibia in the coronal plane is essential in the evaluation and treatment of hindfoot pathologic condition. Previously described standard anteroposterior, lateral, and oblique radiographic methods of the foot or ankle do not demonstrate alignment of the tibia relation to the calcaneus in the coronal plane. The purpose of this study was to introduce hindfoot coronal alignment view. Material : 1) Both feet were imaged simultaneously on an elevated, radiolucent foot stand equipment. 2) Both feet stood on a radiolucent platform with equal weight on both feet. 3) Both feet are located foot axis longitudinal perpendicular to the platform. 4) Silhouette tracing around both feet are made, and line is then drawn to bisect the silhouette of the second toe and the outline of the heel. 5) The x-ray beam is angled down approximately $15^{\circ} to $20^{\circ} Result : 1) This image described tibial axis and medial, lateral tuberosity of calcaneus. 2) Calcaneus do not rotated. 3) The view is showed by talotibial joint space. Conclusion: Although computed tomographic and magnetic resonance imaging techniques are capable of demonstrating coronal hindfoot alignment, they lack usefulness in most clinical situations because the foot is imaged in a non-weight bearing position. But hindfoot coronal alignment view is obtained for evaluating position changing of inversion, eversion of the hindfoot and varus, valgus deformity of calcaneus.

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Surgical Repair of Single Ventricle (Type III C solitus) (단심실 -III C Solitus 형의 수술치험-)

  • naf
    • Journal of Chest Surgery
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    • v.12 no.3
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    • pp.281-288
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    • 1979
  • For years, physicians and anatomists have been interested in the heart that has one functioning ventricle. Various terms have been suggested for this entity including single ventricle, common ventricle, double-inlet left ventricle, cor biatriatum triloculare, and primitive ventricle. In this report, the term "single ventricle" is utilized as suggested by Van Praagh, and is defined as that congenital cardiac anomaly in which a common or separate atrioventricular valves open into a ventricular chamber from which both great arterial trunks emerge. An outlet chamber, or infundibulum, may or may not be present and give rise to the origin of either of the great arteries. This definition excludes the entity of mitral and tricuspid atresia. An 11 year old cyanotic boy was admitted chief complaints of exertional dyspnea and frequent upper respiratory infection since 2 weeks after birth. He was diagnosed as inoperable cyanotic congenital heart disease, and remained without any corrective treatment up to his age of 11 year when he suffered from aggravation of symptoms and signs of congestive heart failure for 2 months before this admission. On 22nd of May 1979, he was admitted for total corrective operation under the impression of tricuspid atresia suggested by a pediatrician. Physical check revealed deep cyanosis with finger and toe clubbing, and grade V systolic ejection murmur with single second heart sound was audible at the left 3rd intercostal space. Development was moderate in height [135 cm] and weight[28Kg]. Routine lab findings were normal except increased hemoglobin [21.1gm%], hematocrit [64 %], and left axis deviation with left ventricular hypertrophy on EKG. Cardiac catheterization and angiography revealed 1-transposition of aorta, pulmonic valvular stenosis, double inlet of a single ventricle with d-loop, and normal atriovisceral relationship [Type III C solitus according to the classification of Van Praagh]. At operation, longitudinal incision at the outflow tract of right ventricle in between the right coronary artery and its branch [LAD from RCA] revealed high far anterior aortic valve which had fibrous continuity with mitral annulus, and pulmonic valve was stenotic up to 4 mm in diameter positioned posterolaterally to the aorta. Ventricular septum was totally defective, and one markedly hypertrophied moderator band originated from crista supraventricularis was connected down to the imaginary septum of the ventricular cavity as a pseudoseptum of the ventricle. Size of the defect was 3X3 cm2 in total. Patch closure of the defect with a Teflon felt of 3.5 x 4 cm2 was done with interrupted multiple sutures after cut off of the moderator band, which was resutured to the artificial septum after reconstruction of the ventricular septum. Pulmonic valvotomy was done from 4 mm to 11 mm in diameter thru another pulmonary arteriotomy incision, and right ventriculotomy wound was closed reconstructing the right ventricular outflow tract with pericardial autograft of 3 x 4 cm2. Atrial septal defect of 2 cm in diameter was closed with 3-0 Erdeck suture, and atrial wall was sutured also when rectal temperature reached from 24`C to 35.5`C. Complete A-V block was managed with temporary external pacemaker with a pacing rate of 110/min. thru myocardial wire, and arterial blood pressure of 80/50 mmHg was maintained with Isuprel or Dopamine dripping under the CVP of 25-cm saline. Consciousness was recovered one hour after the operation when his blood pressure reached 100 /70 mmHg, but vital signs were not stable, and bleeding from the pericardial drainage and complete anuria were persisted until his heart could not capture the pacemaker impulse, and patient died of low output syndrome 320 min after the operation.

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Surgical Repair for Ebstein's Anomaly (Ebstein 기형의 수술 -2례 보고-)

  • naf
    • Journal of Chest Surgery
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    • v.12 no.3
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    • pp.289-296
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    • 1979
  • For years, physicians and anatomists have been interested in the heart that has one functioning ventricle. Various terms have been suggested for this entity including single ventricle, common ventricle, double-inlet left ventricle, cor biatriatum triloculare, and primitive ventricle. In this report, the term "single ventricle" is utilized as suggested by Van Praagh, and is defined as that congenital cardiac anomaly in which a common or separate atrioventricular valves open into a ventricular chamber from which both great arterial trunks emerge. An outlet chamber, or infundibulum, may or may not be present and give rise to the origin of either of the great arteries. This definition excludes the entity of mitral and tricuspid atresia. An 11 year old cyanotic boy was admitted chief complaints of exertional dyspnea and frequent upper respiratory infection since 2 weeks after birth. He was diagnosed as inoperable cyanotic congenital heart disease, and remained without any corrective treatment up to his age of 11 year when he suffered from aggravation of symptoms and signs of congestive heart failure for 2 months before this admission. On 22nd of May 1979, he was admitted for total corrective operation under the impression of tricuspid atresia suggested by a pediatrician. Physical check revealed deep cyanosis with finger and toe clubbing, and grade V systolic ejection murmur with single second heart sound was audible at the left 3rd intercostal space. Development was moderate in height [135 cm] and weight[28Kg]. Routine lab findings were normal except increased hemoglobin [21.1gm%], hematocrit [64 %], and left axis deviation with left ventricular hypertrophy on EKG. Cardiac catheterization and angiography revealed 1-transposition of aorta, pulmonic valvular stenosis, double inlet of a single ventricle with d-loop, and normal atriovisceral relationship [Type III C solitus according to the classification of Van Praagh]. At operation, longitudinal incision at the outflow tract of right ventricle in between the right coronary artery and its branch [LAD from RCA] revealed high far anterior aortic valve which had fibrous continuity with mitral annulus, and pulmonic valve was stenotic up to 4 mm in diameter positioned posterolaterally to the aorta. Ventricular septum was totally defective, and one markedly hypertrophied moderator band originated from crista supraventricularis was connected down to the imaginary septum of the ventricular cavity as a pseudoseptum of the ventricle. Size of the defect was 3X3 cm2 in total. Patch closure of the defect with a Teflon felt of 3.5 x 4 cm2 was done with interrupted multiple sutures after cut off of the moderator band, which was resutured to the artificial septum after reconstruction of the ventricular septum. Pulmonic valvotomy was done from 4 mm to 11 mm in diameter thru another pulmonary arteriotomy incision, and right ventriculotomy wound was closed reconstructing the right ventricular outflow tract with pericardial autograft of 3 x 4 cm2. Atrial septal defect of 2 cm in diameter was closed with 3-0 Erdeck suture, and atrial wall was sutured also when rectal temperature reached from 24`C to 35.5`C. Complete A-V block was managed with temporary external pacemaker with a pacing rate of 110/min. thru myocardial wire, and arterial blood pressure of 80/50 mmHg was maintained with Isuprel or Dopamine dripping under the CVP of 25-cm saline. Consciousness was recovered one hour after the operation when his blood pressure reached 100 /70 mmHg, but vital signs were not stable, and bleeding from the pericardial drainage and complete anuria were persisted until his heart could not capture the pacemaker impulse, and patient died of low output syndrome 320 min after the operation.

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Clinical Significance of Thoracoscopy on Spontaneous Pneumothorax (자연기흉에 대한 Thoracoscopy 의 임상적의의)

  • Kim, Young-Toe;Kim, Kun-Ho
    • Journal of Chest Surgery
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    • v.8 no.1
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    • pp.19-28
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    • 1975
  • The thoracoscopic study was reported on 21 cases of spontaneous pneumothorax requiring surgical management, and clinical values of thoracoscopic examination on spontaneous pneumothorax were also discussed. patients were treated in the Department of .Thoracic Surgery, Hanyang University Hospital for the period of two Years from May 1972 to April 1974. For exact detection of etiologic factors on spontaneous pneumothorax, the thoracoscopic examination in the intrapleural space was performed in parallel with X-ray study. this study, the difference of diagnostic and therapeutic significance between radiological and thoracoscopic findings were observed and compared simultaneously. The results are summerized as follows: Patients age was distributed between 3 and 70 years old with highest incidence in the age group of sixty decade [33. 3%], and sex ratio of male to female was 5:2. The tuberculous processes which developed superficial subpleural layer in the lung parenchyme, on the pulmonary surface could be observed by thoracoscopic examination in a characteristic picture. detection ratio of pulmonary tuberculosis by the radiologic study to that by thoracoscopy was 8:2. The adhesion between the visceral and the parietal pleura which could possibly make a rupture of the alveola and the visceral pleura was found to be localized in a small area of the lung surface. The other part of the lung surface was free of the adhesion and, therefore, the movement of the lung took place completely without any difficulty. The ruptured orifice of the pleura and pathological changes surrounding the orifice can be detected by thoracoscopy, but not by other means such as radiologic examination. A single tuberculous bleb and multiple emphysematous blebs were found on 6 cases out of 21 cases of spontaneous pneumothorax. Among these cases, radiologic Study revealed the bleb only in one patient. On the other hand, the blebs were found in all the six patients by means of thoracoscopic examination. It gives the detection ratio of bleb by radiologic study to that by thoracoscopy was 1:6. By thoracoscopy, the rupture on the lung surface were visualized on the 10 patients out of a total of 21 patients [10 patients of visual rupture]. However, the rupture of the pleura was not observed on the rest of 11 patients even by thoracoscopic examination [11 patients of non visual rupture]. Five patients [50%] out of ten who had the visual rupture on the lung surface was required a surgical operation to remove pneumothorax. For the patients who were detected to have the visual rupture of the pleura by thoracoscopy, be considered in the early stage of closed thoracostomy. of 21 patients, 16 patients [11 patients of non visual rupture of the pleura and 5 patients of visual rupture of the pleura] who received no surgical management, were treated with closed thoracostomy with continuous suction, and the` pneumothorax was healed completely up in each cases. Therapeutic measures for the remaining 5 patients of visual rupture of the pleura who were subjected to surgical approach for radical treatment of spontaneous pneumothorax were accordingly complicated, and the following different procedures were properly indicated case by case, that is, rib resection thoracostomy, simple closure of ruptured visceral pleura, wedged resection of the lung, and lobectomy.

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Effect of Temperature at Flower Blooming Time on the Fertilization and Development of Embryo in the Cultivars of Tongil Line Rice (통일형품종의 개화기 온도가 수정 및 조발육에 미치는 영향)

  • Man-Sang Lee;Nou-Poung Park;Toe-O Kwon;Seok-Hong Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.24 no.1
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    • pp.30-36
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    • 1979
  • The fertilizations of cv Palkweng, Noupoung, and TN 1 which were crossed artificially at 2$0^{\circ}C$ and 3$0^{\circ}C$ were examined. The meiosis and the rate of maturity of the above 3 cultivars, Iri #326, Milyang #29, Suwon #264, and 3 others which were transplated at different times in paddy field were examined, and the results of which were as follows. 1. Palkweng at 3$0^{\circ}C$ was fertilized within 1.5~4.0 hours ,after pollination and at 20\circ C was done within 2.8~4.5 hours after pollination. 2. Noupoung and TNI at 3$0^{\circ}C$ were fertilized within 2.0~5.5 hours after pollination and at 2$0^{\circ}C$ were done within 3.0~6.0 hours after pollination. 3. The rates of fertilization of the cultivated rice plants at 2$0^{\circ}C$ were dropped in general. TN 1 at 2$0^{\circ}C$ was often fertilized abnormally. 4. In the development of embryo, Palkweng was the earliest at 3$0^{\circ}C$ and TN 1 was the earliest at 2$0^{\circ}C$. 5. Although the time of transplant was late, the pollens of Palkweng was normal because the meiosis of that was normal. but the pollens of TNI was sterile in general because of the abnormal meiosis.

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The Influence of Midsole Hardness and Sole Thickness of Sport Shoes on Ball Flex Angle with the Increment of Running Velocity (달리기 속도의 증가에 따른 운동화 중저의 경도와 신발바닥의 두께가 신발의 볼 굴곡각도에 미치는 영향)

  • Kwak, Chang-Soo;Mok, Seung-Han;Kwon, Oh-Bok
    • Korean Journal of Applied Biomechanics
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    • v.15 no.4
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    • pp.153-168
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    • 2005
  • The purposes of this study were to determine the influence of midsole hardness and sole thickness of sports shoes on ball flex angle and position with increment of running velocity. The subjects employed for this study were 10 college students who did not have lower extremity injuries for the last one year and whose running pattern was rearfoot striker of normal foot. The shoes used in this study had 3 different midsole hardness of shore A 40, shore A 50, shore A 60 and 3 different sole thickness of 17cm, 19cm, 21cm. The subjects were asked to run at 3 different speed of 2.0m/sec, 3.5m/sec, 5.0m/sec and their motions were videotaped with 4 S-VHS video cameras and 2 high speed video cameras and simultaneously measured with a force platform. The following results were obtained after analysing and comparing the variables. Minimum angle of each ball flex position were increased with the increment of running velocity and shoe sole thickness(P<0.05), but mid-sole hardness did not affect minimum ball flex angle. The position which minimum angle was shown as smallest was 'D'. Midsole hardness and sole thickness did not affect time to each ball flex minimum angle, total angular displacement of ball flex angle, and total angular displacement of torsion angle(P<0.05). The position which minimum angle was appeared to be earliest was similar at walking velocity, and E and F of midfoot region at running velocity. Total angular displacement of ball flex position tended to increase as shifted to heel. It was found that running velocity had effects on ball flex angle variables, but shoe sole thickness partially affected. It would be considered that running velocity made differences between analysis variables at walking and running when designing shoes. Also, it was regarded that shoes would be developed at separated region, because ball flex angle and position was shown to be different at toe and heel region. It is necessary that midsole hardness and thickness required to functional shoes be analyzed in the further study.

The Effects of Plantar foot Pressure and EMG Activation of Neck, Lumbar and Low Limbs by Using Carrier during Walking (처네(앞.뒤) 사용 방법이 보행 시 목, 허리 및 다리 근육 활성도와 족저압에 미치는 영향)

  • Lee, Sang-Yeol;Chang, Jong-Sung;Lee, Myoung-Hee
    • Korean Journal of Applied Biomechanics
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    • v.19 no.2
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    • pp.237-244
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    • 2009
  • The purpose of this study was to investigate the changing plantar foot pressure and muscle activation of neck, lumbar and low limb by different way of the using carrier during walking. Twenty healthy and young females who brought up infants and had no musculoskeletal disorders of neck, lumbar and low limb were instructed to perform plantar foot pressure and muscle activation of neck, lumbar and low limb during different ways of the using the carrier and walking. Plantar foot pressures were recorded by RS-scan system(RS scan Ltd, German), muscle activation were recorded by ProComp infinitiTM(Thought Technology Ltd, Canada). The data collected by each way of the using carrier and One-way ANOVA was used to analyze. The results indicated that there was a significant increase on erector spinae muscle activation and pressure of great toe zone by using anterior carrier and there was a increase on activation of paraspinal muscle and metatarsal zone by using posterior carrier. Therefore, the way of using carrier could be influenced upon structure and function of the foot and muscle activation.

Plantar Pressure in Skilled and Unskilled Players during Baseball Batting (야구 타격시 숙련자와 미숙련자의 족저압력 분석)

  • Moon, Won-Ho;Lee, Joong-Sook;Kim, Chang-Hyun;Jang, Young-Min;Jeong, Jin-Woo
    • Korean Journal of Applied Biomechanics
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    • v.23 no.1
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    • pp.25-35
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    • 2013
  • This study examined 24 right-handed amateur baseball players. Twelve who had played baseball for more than 6 years were grouped as skilled players, while 12 who had played for 1-3 years were the unskilled player group. The swing motion was divided into four event phases: stance, backswing, impact, and follow-through. The mean and maximum plantar pressure, center of pressure, and ground reaction force were measured during each event phase. The mean and standard deviations for each variables were calculated and differences were validated with the independent sample t-test. A p-value <0.05 was considered statistically significant. The results were as follows. 1)The ideal stance is a stable, balanced position with more than 65% of weight on the right foot. There was significant difference in mean left plantar pressure, while the maximal plantar pressure and mean right plantar pressure did not differ significant. 2)The effective backswing of a skilled player is comprised a rightward shift in weight to build maximum energy. More than 90% of the weight was on the right foot. There was a significant difference in the mean left plantar pressure, while the maximal plantar pressure and mean right plantar pressure did not differ significantly. 3) For an effective impact, a rapid shift in weight to the left foot is essential, so that a power hit is obtained. Significant difference in the mean and maximum plantar pressures of both feet were observed. 4)Follow-through requires wight balance, more on the right than the left, without leaning leftward. There was no significant difference in the mean or maximum plantar pressure. 5)The center of plantar pressure should move from the center of the foot to the toe. 6)The analyses of the ground reaction force suggest that a good swing involves a gradual shift in weight to the right side and a rapid leftward shift at impact. Good balance, with the center of gravity on the right side at follow-through, is also required.

Characteristics of Ice Jam and flow in channel Bends (만곡수로에서의 Ice Jam과 흐름특성)

  • 윤세의
    • Water for future
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    • v.21 no.4
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    • pp.399-406
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    • 1988
  • Presented in this paper is a brief summary of the basic theory and observation from a laboratory investigation aimed at determining flow characteristics and ice jam topography in a sinuous channel, and in a single-bend channel. The sinuous channel comprised thirteen $90^{\circ}$ bends and was of comparatively small s\aspect ratio. The single-bend channel was a $180^{\circ}$ bend, which was an order of magnitude large in width as well as aspect ratios than the sinuous channel. The simulated ices were polyethylene and polypropylene beads and block. The streamwise velocities near the bottom were larger than that of surface in sinuous channel and forming ice jam in sinuous channel, this phenoumena were found strongly. Jams were generally thicker along the inner bank of bends. The path of maximum-streamwise velocity was displaced towards approachs side of the inner bank of bends. Radial variation of jam thickness was to be regular by increasing size of ice fragments. The rate of jam head progression around outer bank of the single bend was faster than that of inner bank and its velocity was roughly steady. With increasing Froude number, jm thickness became less uniformly distributed; being generally thicker along the inner bank and near the jam's toe. Two-layer model might be adaptable for the computing the streamwise velocity in shallow river bends. Two cells of secondary flow cound be expected in ice covered-river bends.

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