• Title/Summary/Keyword: ToFD

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Evaluation of Tinda Gourd (Praecitrullus fistulosu) Germplasm's Yield

  • RAHEEL, Asfand;KHAN, Nasir Ahmad;BABAR, Raheel;ULLAH, Muhammad Arshad;ZAFFAR, Ali;IQBAL, Maouz;ASHRAF, Usman
    • The Korean Journal of Food & Health Convergence
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    • v.5 no.3
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    • pp.1-5
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    • 2019
  • The field experiment was conducted in vegetable area, Institute of Horticultural Sciences in University of Agriculture, Faisalabad in order to recognize morphological and fruit growth pattern and yield of Tinda (Praecitrullus fistulosus) gourd germplasm lines. Sixteen germplasm lines in which one line is used as check are included were sown and grown on flat beds in field. The field experiment was arranged as randomized complete block design (RCBD) with three replications. Data on days to maturity, fruit per vine, fruit weight in grams, fruit diameter and total yield obtained were recorded. The fruit texture is marked as phenotype parameter. All parameters were collected and then analyzed statistically. All lines and replications showed different results among each other according to parameter. The maximum DTM (days to maturity), F/P (fruit per plant), FW (fruit weight), FD (fruit diameter) and Yield were recorded was $L_0$ (68.66), $L_3$ (1.66), $L_{10}$ (248.33), $L_{13}$ (8.50) and $L_0$ (599.33) and the minimum were recorded was $L_1$ (56), $L_{11}$ (0.33), $L_0$ (198), $L_9$ (7) and $L_4$ (421) grams respectively. All lines showed smooth texture of fruits with no hairs when mature at harvesting stage.

Electron Transfer to Hydroxylase through Component Interactions in Soluble Methane Monooxygenase

  • Lee, Chaemin;Hwang, Yunha;Kang, Hyun Goo;Lee, Seung Jae
    • Journal of Microbiology and Biotechnology
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    • v.32 no.3
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    • pp.287-293
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    • 2022
  • The hydroxylation of methane (CH4) is crucial to the field of environmental microbiology, owing to the heat capacity of methane, which is much higher than that of carbon dioxide (CO2). Soluble methane monooxygenase (sMMO), a member of the bacterial multicomponent monooxygenase (BMM) superfamily, is essential for the hydroxylation of specific substrates, including hydroxylase (MMOH), regulatory component (MMOB), and reductase (MMOR). The diiron active site positioned in the MMOH α-subunit is reduced through the interaction of MMOR in the catalytic cycle. The electron transfer pathway, however, is not yet fully understood due to the absence of complex structures with reductases. A type II methanotroph, Methylosinus sporium 5, successfully expressed sMMO and hydroxylase, which were purified for the study of the mechanisms. Studies on the MMOH-MMOB interaction have demonstrated that Tyr76 and Trp78 induce hydrophobic interactions through π-π stacking. Structural analysis and sequencing of the ferredoxin domain in MMOR (MMOR-Fd) suggested that Tyr93 and Tyr95 could be key residues for electron transfer. Mutational studies of these residues have shown that the concentrations of flavin adenine dinucleotide (FAD) and iron ions are changed. The measurements of dissociation constants (Kds) between hydroxylase and mutated reductases confirmed that the binding affinities were not significantly changed, although the specific enzyme activities were significantly reduced by MMOR-Y93A. This result shows that Tyr93 could be a crucial residue for the electron transfer route at the interface between hydroxylase and reductase.

An Estimation Methodology of Empirical Flow-density Diagram Using Vision Sensor-based Probe Vehicles' Time Headway Data (개별 차량의 비전 센서 기반 차두 시간 데이터를 활용한 경험적 교통류 모형 추정 방법론)

  • Kim, Dong Min;Shim, Jisup
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.21 no.2
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    • pp.17-32
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    • 2022
  • This study explored an approach to estimate a flow-density diagram(FD) on a link in highway traffic environment by utilizing probe vehicles' time headway records. To study empirical flow-density diagram(EFD), the probe vehicles with vision sensors were recruited for collecting driving records for nine months and the vision sensor data pre-processing and GIS-based map matching were implemented. Then, we examined the new EFDs to evaluate validity with reference diagrams which is derived from loop detection traffic data. The probability distributions of time headway and distance headway as well as standard deviation of flow and density were utilized in examination. As a result, it turned out that the main factors for estimation errors are the limited number of probe vehicles and bias of flow status. We finally suggest a method to improve the accuracy of EFD model.

Evaluation of Morphological Traits and Genetic Composition in Melon Germplasm (멜론 유전자원의 형태적 특성 및 유전적 구성 평가)

  • Lee, Seungbum;Jang, Ik;Hyun, Do Yoon;Lee, Jung-Ro;Kim, Seong-Hoon;Yoo, Eunae;Lee, Sookyeong;Cho, Gyu-Taek;Lee, Kyung Jun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.4
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    • pp.485-495
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    • 2020
  • Melon (Cucumis melo L.), one of the most important fruit crop species, is cultivated worldwide. In this study, a total of 206 melon accessions conserved at the National Agrobiodiversity Center (NAC) in RDA were characterized for nine morphological characteristics according to the NAC descriptor list. In addition, to confirm the genetic composition of each melon accession, genetic profiling was performed using 20 SSR markers. Among the 206 melon accessions, 159 (77.2%) were collected from Asia. The color of fruit flesh and skin were mostly 'white' (56.0%) and 'green' (49%), respectively. Days to female flowering (FD) and maturity (MD) of the accessions ranged from 58 to 72 and 17 to 63, respectively. The fruit length and width of the accessions ranged from 6.0 to 29.3 and 3.6 to 17.2 cm, respectively. The sugar content (SU) ranged from 2.5% to 13.2% with an average of 7.0%. In correlation analysis, SU showed positive and negative correlations with MD and FD, respectively. The accessions were classified into four clusters by cluster analysis. From the results of genetic profiling using 20 SSR markers, three accessions (K189118, K100486, and K190292) were expected to be inbred lines among 206 melon accessions. These results could expand the knowledge of the melon germplasm, providing valuable material for the development of new melon varieties to suit consumer tastes.

Species classification of the toxic dinoflagellate Alexandrium tamarense and A. catenella based on their paralytic shellfish toxin profiles

  • Kim, Young-Soo;Kim, Chang-Hoon
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.128-128
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    • 2003
  • The annually outbreak of paralytic shellfish poisoning (PSP) were caused by toxic dinolagellate A. tamarense and A. catenella in Korea. The purpose of this study were to investigate the distribution of PSP-causative organisms, A. tamarense and A. catenella and their species classification. Sediment (Saemangeum, the south open sea) and water samples (southeastern coast) were sampled to establish clonal isolates in 2003. After isolation and purification, strains were cultured under $17^{\circ}C$, f/2 media, 14:10=L:D cycle. PST analysis and species identification were performed by HPLC-FD method and specific DNA probe, respectively. Thirty-ons strains were isolated from the Saemangeum reclamation, southeastern coast including Jinhae Bay and south open sea. PSTs were detected in all cultured strains. In eight strains from south offshore, major toxin components are GTX5, C1/2 and minors are GTX3/4, dcGTX3, neoSTX. Sixteen strains from south coastal area have GTX1/4, neoSTX, C1/2 as major toxin components and GTX2/3 as minors. Seven strains from the Saemangeum reclamation have GTX5, C1/2 as major toxin components and GTX1/2/3/4 as minors. Thus, among eight south offshore isolates, four A. tamarense have more toxic (38.31~l19.16 fmol.$cell^{-1}$) than A. catenella (3.78~13.13 fmol.$cell^{-1}$). With the previous results of different toxin composition, toxin components and toxin contents, .it is toxin profile that could used to diagnosis of regional toxic population and geographical distribution of both A. tamarense and A. catenella and their toxigenic strains.

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Single-Crystal Structures of Li+-exchanged Zeolite X (FAU, Si/Al = 1.09) from Aqueous Solution Depends on Ion-exchange Temperatures at 293 and 333 K

  • Kim, Hu-Sik;Ko, Seong-Oon;Lim, Woo-Taik
    • Bulletin of the Korean Chemical Society
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    • v.33 no.10
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    • pp.3303-3310
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    • 2012
  • Two single crystals of fully dehydrated partially $Li^+$-exchanged zeolite X were prepared by the exchange of Na-X, $Na_{92}Si_{100}Al_{92}O_{384}$ (Si/Al = 1.09), with $Li^+$ using aqueous 0.1 M $LiNO_3$ at 293 (crystal 1) and 333 K(crystal 2), followed by vacuum dehydration at 623 K and $1{\times}10^{-6}$ Torr for 2 days. Their structures were determined by single-crystal synchrotron X-ray diffraction techniques in the cubic space group $Fd{\overline{3}}$ at 100(1) K. Their structures were refined using all intensities to the final error indices (using the 1281 and 883 reflections for which ($F_o$ > $4{\sigma}(F_o)$) $R_1/R_2$ = 0.075/0.244 and 0.074/0.223 for crystals 1 and 2, respectively. Their compositions are seen to be ${\mid}Li_{86}Na_6{\mid}[Si_{100}Al_{92}O_{384}]$-FAU and ${\mid}Li_{87}Na_5{\mid}[Si_{100}Al_{92}O_{384}]$-FAU, respectively. In crystal 1, 17 $Li^+$ ions per unit cell are at site I', 15 another site I', 30 at site II, and the remaining 16 at site III; 2 $Na^+$ ions are at site II and the remaining 4 at site III'. In crystal 2, 32 and 30 $Li^+$ ions per unit cell fill sites I' and II, respectively, and the remaining 25 at site III'; 2 and 3 $Na^+$ ions are found at sites II and III', respectively. The extent of $Li^+$ exchange increases slightly with increasing ion exchange temperature from 93% to 95%.

Influences of changes in the Thermal Properties on Pyrolysis of Solid Combustibles (열물성의 변화가 고체 가연물의 열분해에 미치는 영향)

  • Hong, Ter-Ki;Lee, Jong Won;Park, Seul-Hyun
    • Fire Science and Engineering
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    • v.31 no.3
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    • pp.41-48
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    • 2017
  • In order to investigate the influence of changes in the thermal properties of solid combustibles on thermal decomposition, a series of solid pyrolysis experiments were performed using a cone calorimeter specified in KS F ISO 5660-1. In the present study, Poly Methyl Methacrylate (PMMA) which does not produce Char during pyrolysis process was used as solid fuel. Results obtained from cone calorimeter experiments were compared to ones obtained from numerical analysis of Fire Dynamics Simulator (FDS) 1D pyrolysis model adopted with thermal properties of solid fuel as input parameters. Comparisons between experimentally calculated and model-predicted mass loss rate were then made to elucidate the effect of changes in the thermal properties on pyrolysis of PMMA.

Analysis of Human Exposure to Wideband Pulse EMF Dependent on the Dispersion Algorithm (분산 알고리즘에 따른 광대역 펄스 전자파 노출에 대한 인체 노출량 해석)

  • Ka Won-Suk;Kim Tae-Hong;Kim Jeong-Ran;Pack Jeong-Ki
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.17 no.7 s.110
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    • pp.684-693
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    • 2006
  • In this paper, the SA (Specific Absorption) distribution in a human head, exposed to wideband pulse EMF, has been analyzed by taking into account the dispersion characteristics of biological tissues. The dispersive properties of biological tissues are characterized by the 4th Cole-Cole model. Currently, there is no dispersive FDTD algorithm to implement the 4th Cole-Cole model accurately. Thus, in this paper the FDTD methods with the dispersive algorithm for the 1st-order Cole-Cole model and the 3rd-order Debye model were used for SA analysis. The validity of each model has been investigated first, and then the effects of dispersion on SA distribution have been studied.

Current situation and future prospects for the Australian beef industry - A review

  • Greenwood, Paul L;Gardner, Graham E;Ferguson, Drewe M
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.7
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    • pp.992-1006
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    • 2018
  • Beef production extends over almost half of Australia, with about 47,000 cattle producers that contribute about 20% ($A12.7 billion gross value of production) of the total value of farm production in Australia. Australia is one of the world's most efficient producers of cattle and was the world's third largest beef exporter in 2016. The Australian beef industry had 25 million head of cattle in 2016-17, with a national beef breeding herd of 11.5 million head. Australian beef production includes pasture-based cow-calf systems, a backgrounding or grow-out period on pasture, and feedlot or pasture finishing. Feedlot finishing has assumed more importance in recent years to assure the eating quality of beef entering the relatively small Australian domestic market, and to enhance the supply of higher value beef for export markets. Maintenance of Australia's preferred status as a quality assured supplier of high value beef produced under environmentally sustainable systems from 'disease-free' cattle is of highest importance. Stringent livestock and meat quality regulations and quality assurance systems, and productivity growth and efficiency across the supply chain to ensure price competiveness, are crucial for continued export market growth in the face of increasing competition. Major industry issues, that also represent research, development and adoption priorities and opportunities for the Australian beef industry have been captured within exhaustive strategic planning processes by the red meat and beef industries. At the broadest level, these issues include consumer and industry support, market growth and diversification, supply chain efficiency, productivity and profitability, environmental sustainability, and animal health and welfare. This review provides an overview of the Australian beef industry including current market trends and future prospects, and major issues and opportunities for the continued growth, development and profitability of the industry.

Reverse Parking Guidance System with the Path Prediction (경로 예측 방식의 후진 주차 가이드 시스템 개발)

  • Ryu, Dae-Hyun;Lee, Duk Woo;Choi, Hyo-Sun;Choi, Taewan
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.8
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    • pp.4006-4011
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    • 2013
  • Reverse parking guidance system help driver's safe reverse parking with display of the expected reverse path of the car on portable navigation device or rear-view camera display. In this paper, we suggest a reverse path prediction method of vehicle which reflect the differences in steering angle according to various types of vehicles and calibrate easily the distance error according to the property and the installation position of rear-view camera and we developed reverse parking guidance system with the our reverse path prediction method. This system can be applied to various types of vehicles, a variety of characteristics and installation of rear-view camera and navigation support systems, or portable devices are compatible and easily detachable, can be configured easily.