• Title/Summary/Keyword: Tissue transplantation

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THE LONG-TERM GROWTH OF HOMOGENEOUS EMBRYO TOOTH GERM TRANSPLANTED INTO THE MAXILLA OF A RAT (흰쥐의 악골에 동종 이식한 태아 치아싹의 장기간 발육)

  • Ko, Dong-Hyun;Chung, Han-Sung;Kim, Seong-Oh;Lee, Jae-Ho;Choi, Hyung-Jun;Choi, Byung-Jai
    • Journal of the korean academy of Pediatric Dentistry
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    • v.34 no.1
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    • pp.53-61
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    • 2007
  • In case of missing tooth caused by dental caries or periodontal disease, it can be restored by various methods, and there has been much interest in implant and tooth transplantation. The success of tooth transplantation is going to be attained through the knowledge of growth, development and calcification of tooth. Tooth transplantation has been experimented in vivo and in vitro. Many animals such as rats, mice, cats and dogs are used for tooth transplantation experiment in vivo. In most experiments, tooth was transplanted into the extraoral site, but rare into the intraoral site In this study, to observe the capacity of formation and mineralization of tooth germ, first molar of a matured white rat was extracted and the cap stage tooth germ of a 13.5 Embryonic day embryo rat was transplanted into the extracted socket. The rats were killed 6 months later and the radiographical and histological results are as followings. 1. Tooth germ transplanted for 2 and 6 months are developing calcified tooth material such as dentin, cementum, pulp tissue, and epithelium around enamel space in the maxilla was seen. 2. The epithelium around enamel space was located beneath the oral epithelium and contained connective tissue and periodontal ligament. 3. Tooth formation was progressed as transplantation period but the size of newly formed tooth was small and the shape of tooth was incomplete.

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Differentiation and Labeling of Mouse Preadipocytes for Allogenic Transplantation Study (동종이식 연구를 위한 마우스 지방전구세포의 표지 및 분화 방법의 확립)

  • Kim, In Ok;Kim, Taek Seung;Kim, Mi Hyung;Hyon, Won Sok;Mun, Goo Hyun;Oh, Kap Sung;Bang, Sa Ik
    • Archives of Plastic Surgery
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    • v.32 no.4
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    • pp.533-538
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    • 2005
  • Due to its safety and softness, autologous fat transplantation has been commonly performed for soft tissue correction. However, the injected fat is absorbed resulting in the reduction of volume of the graft by 40-60% within a few months. Thus, there was an attempt to use adipocytes differentiated from preadipocytes in vitro for transplantation. Differentiated adipocytes were biocompatible and matured with gradual volume increase at transplantation site in clinical study(unpublished data). In addition, they did not induce immune rejection in response to nonself lymphocytes in a mixed lymphocyte reaction(MLR)(unpublished data). The purpose of this study is to differentiate mouse preadipocytes following labeling into adipocytes to establish an animal model for allogenic transplantation. Preadipocytes isolated from inguinal and retroperitoneal fat pad of C57BL/6 mice were proliferated with growth medium by passage 3 and differentiated into adipocytes with different culture conditions after labeled with BrdU. At most suitable conditions, above 90% of preadipocytes were differentiated and BrdU labeling did not affect differentiation rate and function of differentiated adipocytes. These results demonstrate that BrdU-labeled adipocytes resulting from this in vitro differentiation protocol are useful for allogenic transplantation study.

Influence of Interferon-${\gamma}$ Deficiency in Immune Tolerance Induced by Male Islet Transplantation

  • Kim, Yong-Hee;Lim, Young-Kyoung;Park, Chung-Gyu
    • IMMUNE NETWORK
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    • v.11 no.6
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    • pp.358-363
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    • 2011
  • Background: Traditionally, interferon-${\gamma}$ (IFN-${\gamma}$) was regarded as a pro-inflammatory cytokine, however, recent reports suggested role of IFN-${\gamma}$ in immune tolerance. In our previous report, we could induce tolerance to male antigen (HY) just by male islet transplantation in wild type C57BL/6 mice without any immunological intervention. We tried to investigate the influence of IFN-${\gamma}$ deficiency on tolerance induction by male islet transplantation. Methods: To examine the immunogenicity of male tissue in the absence of IFN-${\gamma}$, we transplanted male IFN-${\gamma}$ knock-out (KO) skin to female IFN-${\gamma}$ KO mice. Next, we analyzed male IFN-${\gamma}$ KO islet to streptozotocin-induced diabetic female IFN-${\gamma}$ KO mice. And, we checked the functionality of grafted islet by graft removal and insulin staining. Results: As our previous results in wild type C57BL/6 mice, female IFN-${\gamma}$ KO mice rejected male IFN-${\gamma}$ KO skin within 29 days, and did not reject male IFN-${\gamma}$ KO islet. The maintenance of normal blood glucose level was dependent on the presence of grafted male islet. And the male islet recipient did not reject 2nd challenge of male islet graft also. Conclusion: Deficiency of IFN-${\gamma}$ does not have influence on the result of male skin graft and male islet transplantation. Conclusively, male islet transplantation induced T cell tolerance is not dependent on the presence of IFN-${\gamma}$.

Recent Applications of Polymeric Biomaterials and Stem Cells in Tissue Engineering and Regenerative Medicine (고분자 생체재료와 줄기세포를 이용한 조직공학과 재생의학의 최신 동향)

  • Lee, Sang Jin;Yoo, James J.;Atala, Anthony
    • Polymer(Korea)
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    • v.38 no.2
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    • pp.113-128
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    • 2014
  • Tissue engineering and regenerative medicine strategies could offer new hope for patients with serious tissue injuries or end-stage organ failure. Scientists are now applying the principles of cell transplantation, material science, and engineering to create biological substitutes that can restore and maintain normal function in diseased or injured tissues/organs. Specifically, creation of engineered tissue construct requires a polymeric biomaterial scaffold that serves as a cell carrier, which would provide structural support until native tissue forms in vivo. Even though the requirements for scaffolds may be different depending on the target applications, a general function of scaffolds that need to be fulfilled is biodegradability, biological and mechanical properties, and temporal structural integrity. The scaffold's internal architecture should also enhance the permeability of nutrients and neovascularization. In addition, the stem cell field is advancing, and new discoveries in tissue engineering and regenerative medicine will lead to new therapeutic strategies. Although use of stem cells is still in the research phase, some therapies arising from tissue engineering endeavors that make use of autologous adult cells have already entered the clinic. This review discusses these tissue engineering and regenerative medicine strategies for various tissues and organs.

A Clinical Study of Free Vascularized Osteocutaneous Fibular Transplantation in Infected Nonunion of Tibia (감염성 경골 불유합에 시행한 혈관 부착 유리 피부편 및 생비골 이식 수술의 임상적 고찰)

  • Song, Joon-Min;Kim, Jin-Ill;Kwon, Hee;Yoo, Jae-Eung;Park, Jong-Suk;Rah, Soo-Kyoon;Choi, Chang-Uk
    • Archives of Reconstructive Microsurgery
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    • v.9 no.1
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    • pp.27-36
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    • 2000
  • February 1995 to September 1999, authors have experienced seven cases of infected nonunion of tibial fractures with associated soft tissue injury and skin defect, and have accomplished union in all cases by free vascularized fibular graft. All grafts healed with no radiographic evidence of bone necrosis or resorption and have been able to treat large bony defect and skin defect simultaneously. In this study, five cases of vascularized free fibular osteocutaneous flap transfer and two cases of free fibular graft are reported. All of seven cases were infected nonunion of tibia. The results were obtained as follows 1) The mean duration of the radiologic bone union was average 5.3months. 2) Grafted fibular has been hypertrophied, average 10.6 months. 3) In five cases of preservation of posterior cortex of tibia, bony union and hypertrophy of grafted bone were earlier than that two cases of complete segmental resection of tibia. 4) In two cases which only free vascularized fibular graft were performed because achievement of cutaneous flap was failed, authors found that soft tissue defect was filled with granulation tissue and split-thickness skin graft was possible over the granulation tissue after 3 weeks postoperatively.

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Regeneration of Bovine Mammary Gland in Immunodeficient Mice by Transplantation of Bovine Mammary Epithelial Cells Mixed with Matrigel

  • Park, Hyun Jung;Lee, Won Young;Jeong, Ha Yeon;Song, Hyuk
    • International Journal of Stem Cells
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    • v.9 no.2
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    • pp.186-191
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    • 2016
  • Background and Objectives: With the global demand for dairy protein for consumption growing annually, there has been increasing activity in the research field of dairy protein synthesis and production. From a manipulation perspective, it is more difficult to use live cattle for laboratory studies on the production of milk as well as of dairy protein such as casein, as compared with using laboratory animals like rodents. Therefore, we aimed to develop a mouse model of bovine mammary alveolar ducts for laboratory-scale studies. We studied the formation of the bovine mammary gland ductal structure by transplanting the MAC-T bovine alveolar cell line into mice. Methods and Results: MAC-T cells ($1{\times}10^7$) were suspended in Matrigel and injected into the dorsal tissue of 8-week-old male BALB/C nude mice. Histological analysis of tissue dissected from the MAC-T cell-transplanted mice after 6 weeks showed the typical morphology of the tubuloalveolar female gland, as well as glands made up of branching ducts that were surrounded by smooth muscle with small alveoli budding off the ducts. In addition, the epithelial markers CK14 and CK18 were expressed within the duct-like structure. Prolactin was detected in the duct interior in these CK14+ and CK18+ cells but not in the non-transplanted MAC-T cells. Conclusions: These results showed that duct-like tissue had been successfully formed after 6 weeks of transplantation of the CK14+ and CK18+ MAC-T cells into mice dorsal tissue. This mouse model will be a useful tool for further research on the bovine mammary gland.

Sensory Function Recovery by Free Tissue Transfer in the Extremities (사지에서 유리 조직 이식술에 의한 감각 기능 회복)

  • Lee, Jun-Mo;Kim, Kwon-Il;Hwang, Byung-Yun
    • Archives of Reconstructive Microsurgery
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    • v.14 no.1
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    • pp.14-17
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    • 2005
  • Purpose: Authors have performed free tissue transplantation in the upper and lower extremities with sensory flaps and evaluated the sensory function recovery. Materials and methods: Between 1992 through 2004, sensory free flap articles published in the journal of the Korean microsurgical society, were reviewed and recovery of sensory function was assessed by static two-point discrimination test. Results: Static two point discrimination test showed average 6.7 mm in the thumb, average 12 mm in the hand and 7 cm of the dorsalis pedis flap, 20.5 mm of the lateral arm flap and over 8 cm of the forearm flap in the foot. Conclusion: Sensory flaps provide the protective and useful coverage in the upper and lower extremities and have benefit for activities for daily life in free tissue transferred patients.

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Salvage Reconstruction of the Knee using Latissimus Dorsi Myocutaneous Free Flap (광 배 근피 유리 판을 이용한 슬관절 구제 재건술)

  • Lee, Jun-Mo;Lee, Ju-Hong;Choi, Moon-Ki
    • Archives of Reconstructive Microsurgery
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    • v.11 no.2
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    • pp.167-172
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    • 2002
  • The only treatment method for crushed soft tissue injuries in the proximal leg involving the knee joint is the microsurgical free flap transplantation, especially latissimus dorsi myocutaneous free flap is useful to cover the extensive soft tissue defects, therefore prevents iatrogenic below knee amputation and facilitates early wound healing, early ambulation and shortens hospital stay. Authors have treated the open amputation with crushed soft tissue and bone injuries in the proximal leg, and the repeated abrasion and infectious wound with traumatic neuroma in the below knee with myocutaneous free flap and succeeded to wear below knee amputation prosthesis and return to normal activities of the daily living in the relatively shorter period than usual.

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Hyperplastic Autotransplanted Parathyroid Tissue Migrating into Fatty Tumor after Total Parathyroidectomy

  • Reza, Joseph Arturo;Wiese, Georg Kristof;Portoghese, Joseph Dominic
    • Journal of Endocrine Surgery
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    • v.18 no.4
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    • pp.236-239
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    • 2018
  • Secondary hyperparathyroidism (SHPTH) occurs commonly in patients with end-stage renal disease (ESRD). Uncontrolled SHPTH is associated with complications of calcium deposition including calciphylaxis and elevated rates of cardiovascular morbidity. Current treatment recommendations for medically refractory disease include total parathyroidectomy, often with autotransplantation (TPTH+AT) of minced parathyroid gland. Surgical intervention is associated with a reduction in cardiovascular mortality. We report a case of a 56-year-old man with ESRD who developed SHPTH and underwent TPTH+AT of parathyroid tissue into the right brachioradialis muscle. Over the course of 7 years he developed a mass at the site of the autotransplanted gland as well as recurrent refractory hyperparathyroidism with increased forearm uptake noted on sestamibi scan. After excision of this mass, pathology demonstrated hyperplasia of the minced gland fragments which were embedded within a mass of fibroadipose tissue rather than the muscle tissue it was originally transplanted in.