• Title/Summary/Keyword: Tissue pattern

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Tissue- and maturity-dependent expression pattern of androgen receptor mRNA in goldfish, Carassius auratus

  • Choi, Cheol-Young;Kim, Soon-Hag;Kim, Bong-Seok
    • Journal of fish pathology
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    • v.16 no.2
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    • pp.111-118
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    • 2003
  • Androgen plays an important role in the regulation of gonadotropin production in vertebrates . We have investigated the transcriptional pattern of androgen receptor (AR) in a variety of tissues in maturing male and female goldfish by RT-PCR. Specific primer for AR was designed based on goldfish AR gene from the GenBank (accession number AY090897). AR was shown 10 be maturity- and tissue-dependent gene expression pattern in goldfish. In immature male goldfish, significantly higher transcript level of AR was observed in the pituitary und testis , compared [0 brain and liver. Mature male goldfish showed a similar expression pattern to immature male goldfish. Interestingly. when compare to male goldfish, female goldfish showed AR mRNA expression that was found 10 be weak in pituitary, and very low expression in brain. They could not be found 10 have expression in any other tissues. Taken together. the- transcriptional analysis of AR depending on the tissue, sex. and maturity of a goldfish provides the opportunity for the study of goldfish reproductive physiology ,The results provided for the first time a comparison of the tissue distribution of AR mRNA in sexually maturating male and female goldfish.

A STUDY ON ELECTROPHORETIC DIFFERENTIATION OF RADICUAR CYSTS AND GRANULOMAS (전기영동분석법(電氣泳動分析法)에 의(依)한 치근단낭종((齒根端囊腫)과 치근단육아종(齒根端肉牙腫)의 감별(鑑別)에 관(關)한 연구(硏究))

  • Bae, Kwang-Shik
    • Restorative Dentistry and Endodontics
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    • v.2 no.1
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    • pp.20-26
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    • 1976
  • Cellulose acetate electrophoresis was used for differential diagnosis of radicular cysts and granulomas. Sixteen periapical lesions were excised from sixteen teeth with radiographic evidence of periapical pathosis, and two normal periodontal tissues were surgically removed from two wisdom teeth. The tissue samples were separated into two parts. Half was prepared for cellulose acetate electrophoresis, and the electrophoretic patterns were scanned with Helena densitometer. The other half was examined histologically, and the histologic diagnoses were then compared with the electrophoretic patterns and with the x-ray findings. The results were the following: 1. The histopathologic results showed the presence of 13 granulomas (81.8%) and 3 cysts (18.3%). 2. An albumin pattern, alpha-globulin pattern, beta-globulin pattern, fibrinogen pattern and gamma-globulin pattern were found in all eighteen cases. 3. All eighteen cases were divided into normal periodontal tissue group, dental granuloma group, and radicular cyst group by the histopathologic diagnosis. The electrophoretic pattern of dental granuloma group and that of normal periodontal tissue group resembled each other. The two groups showed the highest percentage in beta-globulin fraction, and the beta-globulin of dental granuloma group(47.17%) was higher than that of normal periodontal tissue group (40.54%) by 6.63%. On the contrary, beta-globulin fraction of radicular cyst group (28.00%) was much lower than those of the granuloma group (47.17%) and normal periodontal tissue group (40.54%), and alpha-globulin of radicular cyst group (34.16%) was much higher than those of the granuloma group (20.04%) and normal group (19.58%). 4. Five cases (31.3%) of the sixteen periodontal lesions showed different results between histopathologic diagnoses and radiographic findings.

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A Study on Comparative Analysis of Mammography and Tc-99m MIBI Scintimammography for Dense Breast (치밀형 유방에서 유방특이감마영상검사의 유용성 평가)

  • Jeong, Eun-Mi;Kim, Ho-Seong
    • The Korean Journal of Nuclear Medicine Technology
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    • v.16 no.1
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    • pp.76-79
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    • 2012
  • Purpose: This study was to evaluate usefulness of $^{99m}Tc$-MIBI scintimammography for dense breast by comparing concordance in test results between Tc-99m MIBI scintimammography and mammography whose effect was proved the most as an imaging tool depending on breast density and at the same time by examining limitation on evaluation depending on density of breast tissue. [Materials and Methods] In the period from December 2010 to July 2011, this study targeted 150 patients who took both of $^{99m}Tc$-MIBI scintimammography and mammography conducted by using breast gamma camera in this hospital. Breast density was classified to the four levels of pattern 1~4 based on the results of mammography. $^{99m}Tc$-MIBI scintimammography was conducted with the LCC, the RCC, the LMLO, and the RML one minute after intravenous injection of 99mTc-MIBI 7400 MBq (20 mCi) while analysis was made for concordance in test results of $^{99m}Tc$-MIBI scintimammography and mammography. [Results] Among the 150 patients, pattern 1 was found in 3 patients, pattern 2 in 44 patients, pattern 3 in 61 patients, and pattern 4 in 37 patients. There were 5 patients who showed the case where it was impossible to determine density of breast tissue due to foreign body inserted to breast. The concordance ratio of the results between $^{99m}Tc$-MIBI scintimammography and mammography was 95.5% for pattern 2, 95.1% for pattern 3 and 94.6% for pattern 4. This demonstrated that the concordance rate decreased according to the increase in breast density. [Conclusion] When there was limitation on evaluation of breast specific gamma imaging test results due to increased intake in breast tissue or surgical site, the concordance rate was 6.8% for pattern 2, 16.3% for pattern 3 and 18.9% for pattern 4. This demonstrated that the degree of limitation on evaluation of breast specific gamma imaging test results increased according to the increase in breast density.

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척추동물의 Isozyme에 관한 비교연구: III. 한국산 뱀목의 Lactate 및 Malate Dehydrogenase Isozyme

  • 김순옥;조동현;박상윤
    • The Korean Journal of Zoology
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    • v.16 no.1
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    • pp.55-65
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    • 1973
  • MDH and LDH isozymes of various tissues of serveral snakes which belong to Colubridae, Crotalidae and Viperidae were studied. MDH isozymes were appeared as two bands in all species. Four LDH isozymes were found inall species of Colubridae, Crotalidae and Viperidae. LDH isozymes of muscle and liver had fundamentally the same pattern. On the other hand, there were two LDH isozymes in the heart of all species and the pattern of heart LDH isozyme was reverse of the pattern in muscle. The pattern of LDH isozymes of stomach tissue can be thought as the same pattern with that of heart in all species, but in the stomach tissue of Crotalidae and Viperidae there were four LDH isozymes.

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Imaging Features of Soft-Tissue Calcifications and Related Diseases: A Systematic Approach

  • Hwang, Zhen-An;Suh, Kyung Jin;Chen, Dillon;Chan, Wing P.;Wu, Jim S.
    • Korean Journal of Radiology
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    • v.19 no.6
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    • pp.1147-1160
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    • 2018
  • Soft-tissue calcification refers to a broad category of lesions. Calcifications are frequently identified by radiologists in daily practice. Using a simple algorithm based on the distribution pattern of the lesions and detailed clinical information, these calcified lesions can be systematically evaluated. The distribution pattern of the calcific deposits enables initial division into calcinosis circumscripta and calcinosis universalis. Using laboratory test results (serum calcium and phosphate levels) and clinical history, calcinosis circumscripta can be further categorized into four subtypes: dystrophic, iatrogenic, metastatic, and idiopathic calcification. This pictorial essay presents a systematic approach to the imaging features of soft-tissue calcifications and related diseases.

Relationship between Visceral Adipose Tissue and Oriental Obesity Pattern Identification in Obese Korean Women (비만여성에서 내장지방과 한방비만변증의 연관성)

  • Kim, Eun-Joo;Lee, A-Ra;Hwang, Mi-Ja;Cho, Jae-Heung;Choi, Sun-Mi;Chung, Seok-Hee;Song, Mi-Yeon
    • Journal of Korean Medicine Rehabilitation
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    • v.21 no.2
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    • pp.279-288
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    • 2011
  • Objectives : Our purpose of this study was to evaluate the association between abdominal adipose tissue and oriental obesity pattern identification. Methods : This study was performed in 78 healthy obese(BMI(body mass index)${\geq}25kg/m^2$) women in Seoul. Subjects underwent abdominal CT(computed tomography) scanning and were asked to complete the oriental obesity pattern identification questionnaire. Subjects were given written consent and this study was performed under permission of institutional review board of Kyung-hee East-west Neo medical center. Results : 1. VFA(visceral fat area) and VSR(visceral/subcutaneous adipose tissue ratio) were significantly correlated with stagnation of the liver qi(肝鬱, gan-yu)(p<0.05). But, other patterns were not significantly correlated with oriental obesity pattern identification(p>0.05). 2. We evaluated a difference of oriental obesity pattern identification score in visceral obesity group(n=34) versus non-visceral obesity group(n=44). Scores of all patterns except food accumulation(食積, shi-ji) were significantly higher in the visceral obesity group than in the non-visceral obesity group(p<0.05). Especially, there was a further significant difference in stagnation of the liver qi(肝鬱, gan-yu)(p<0.01). Conclusions : Generally, the stagnation of the liver qi(肝鬱, gan-yu) has a close relation with stress and depression. This study suggests that stress and depression might be correlated with visceral fat, and the use of oriental obesity pattern identification would be helpful for planning a treatment schedule of visceral obesity in the clinic.

Robust Extraction of Lean Tissue Contour From Beef Cut Surface Image

  • Heon Hwang;Lee, Y.K.;Y.r. Chen
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1996.06c
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    • pp.780-791
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    • 1996
  • A hybrid image processing system which automatically distinguished lean tissues in the image of a complex beef cut surface and generated the lean tissue contour has been developed. Because of the in homegeneous distribution and fuzzy pattern of fat and lean tissue on the beef cut, conventional image segmentation and contour generation algorithm suffer from a heavy computing requirement, algorithm complexity and poor robustness. The proposed system utilizes an artificial neural network enhance the robustness of processing. The system is composed of pre-network , network and post-network processing stages. At the pre-network stage, gray level images of beef cuts were segmented and resized to be adequate to the network input. Features such as fat and bone were enhanced and the enhanced input image was converted tot he grid pattern image, whose grid was formed as 4 X4 pixel size. at the network stage, the normalized gray value of each grid image was taken as the network input. Th pre-trained network generated the grid image output of the isolated lean tissue. A training scheme of the network and the separating performance were presented and analyzed. The developed hybrid system showed the feasibility of the human like robust object segmentation and contour generation for the complex , fuzzy and irregular image.

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Perforating patterns of cutaneous perforator vessels in anterolateral thigh flaps for head and neck reconstruction and clinical outcomes

  • Lee, Sang Soo;Hong, Jong Won;Lee, Won Jae;Yun, In-Sik
    • Archives of Craniofacial Surgery
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    • v.23 no.2
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    • pp.64-70
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    • 2022
  • Background: Anterolateral thigh (ALT) flaps are versatile soft tissue flaps that have become the standard soft-tissue flaps used for head and neck reconstruction. They provide a long vascular pedicle, constant vessel diameter, abundant soft tissue coverage, and minimal donor site morbidity. The ALT flap was initially designed on the basis of a septocutaneous (SC) perforator. However, more recent research has shown that a substantial number of ALT flaps are now based on musculocutaneous (MC) perforators, and the ratio between MC and SC perforators varies among studies. In this study, we analyzed the perforating pattern of ALT flaps along with their clinical outcomes during head and neck reconstruction in the Korean population. Methods: From October 2016 to July 2020, 68 patients who had undergone an ALT flap procedure for head and neck reconstruction were enrolled retrospectively. The perforating pattern of the cutaneous perforator vessel (MC perforator/SC perforator/oblique branch), pedicle length, and flap size were analyzed intraoperatively. Patient demographics and flap necrosis rates were also calculated. Results: The highest number of cutaneous perforator vessels supplying the ALT flap were the MC perforators (87%). The proportion of MC perforators was significantly higher than that of the SC perforators and oblique branches. Flap necrosis occurred in seven cases (11.86%); sex, hypertension, diabetes mellitus, coronary artery disease, perforator course, and history of radiotherapy did not significantly affect flap necrosis. Conclusion: The ALT free flap procedure remains popular for reconstruction of the head and neck. In this study, we observed that the majority of cutaneous vessels supplying the flaps were MC perforators (87%). When using the MC perforator during flap elevation, careful dissection of the perforator is required to achieve successful ALT flaps because intramuscular dissection is difficult. Perforator pattern and history of radiotherapy did not affect flap necrosis.

Development of Mobile Type Computer Vision System and Lean Tissue Extraction Algorithm for Beef Quality Grading (쇠고기 등급판정을 위한 이동형 컴퓨터시각 장치 및 살코기 추출 알고리즘 개발)

  • Choi S.;Huan Le Ngoc;Hwang H.
    • Journal of Biosystems Engineering
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    • v.30 no.6 s.113
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    • pp.340-346
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    • 2005
  • Major quality features of the beef carcass in most countries including Korea are size, marbling state of the lean tissue, color of the fat and lean tissue, and thickness of back fat of the 13th rib. To evaluate the beef quality, extracting loin parts from the sectional image of the 13th beef rib is crucial and is the first step. However, because of the inhomogeneous distribution and fuzzy pattern of the fat and lean tissues on the beef cut, it is difficult to extract automatically the proper contour of the lean tissue. In this paper, a prototype mobile beef quality measurement system, which can be implemented practically at the beef processing site was developed. The developed system was composed of the hand held image acquisition unit and mobile processing unit mounted with touch-pad screen. Algorithms to extract the boundary of the lean tissue and a proper tool to evaluate the marbling status have been developed using color image processing. The boundary extraction algorithm showed successful results for the beef cuts with simple and moderate patterns of the lean tissue and fat. However, it had some difficulty in eliminating complex pattern of the extraneous tissues adhered to the lean tissue in the boundary extraction. The developed algorithms were implemented to the prototype mobile processing unit.