• 대한의생명과학회지
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• 제9권1호
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• pp.37-42
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• 2003

The present study was conducted to determine whether administration of heat extract of Lepidii Semen has an inhibitory effect on neutrophil-derived oxidative injury following dermal scald burn in rats. Acute lung injury was induced by scald burn (15% of TBSA) in rats. To identify acute edematous lung injury, protein concentrations and numbers of polymorphonuclear leukocytes were measured in bronchoalveolar lavage (BAL) at 5 h after skin burn. In addition, the level of lung KC (neutrophil chemoattractant cytokine) and activity of lung myeloperoxidase (MPO) were measured, and histopathological changes were observed as well. Lung weight and concentration of BAL protein, the index of lung injury, were clearly increased at 5 h postburn compared with those of sham-operated group. Administration of heat extract of Lepidii Semen after scald burn inhibited the production of KC in lung tissue and decreased the activity of lung MPO related to infiltration of neutrophils. In histopathological changes in lung tissue, infiltration of inflammatory cells and pulmonary edema induced by skin burn were decreased by administration of heat extract of Lepidii Semen after scald burn. These results suggest that Lepidii Semen may be an effective medical stuff for acute lung injury induced by skin burn.

• Current Optics and Photonics
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• 제3권2호
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• pp.150-153
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• 2019

Larynx cancer is a potentially terminal and severe type of neck and head cancer in which malignant cells start to grow and spread upwards in the larynx, or voice box. Smoking tobacco, drinking hot beverages and drinking alcohol are the main risk factors for these tumors. In this study, we aimed to develop a precise, accurate and rapid chemometrics assisted Raman spectroscopy method for diagnosis of larynx cancer in deparaffinized tissue samples. In the proposed method, samples were deparaffinized and 20 microns of each tissue were located on a coverslip. Both healthy (n = 13) and cancerous tissues (n = 13) were exposed to a Raman laser (785 nm) and excitations were recorded between wavenumbers of $50{\sim}1500cm^{-1}$. An Orthogonal Partial Least Square algorithm was applied to evaluate the Raman spectrum obtained. Sensitivity and specificity of the proposed method is high enough with the aid of Principal Component Analysis (PCA) to test the whole model. Healthy and cancerous tissues were accurately and precisely clustered. A rapid, easy and precise diagnosis algorithm was developed for larynx cancer. By this method, some useful data about differences in biomolecules of each group (phospholipids, amides, tyrosine, phenylalanine collagen etc.) was also obtained from the spectra. It is claimed that the optimized method has a great potential for clustering and separating tumor tissues from healthy ones. This novel, rapid, precise and objective diagnosis method may be an alternative for the conventional methods in literature for diagnosis of larynx cancer.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.43-43
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• 2003

This study was conducted to produce transgenic pig harboring human tissue plasminogene activator (tPA) gene. Two different tPA genes containing bovine $\beta$-casein promoter and mouse uroplakin promoter were prepared for microinjection and confirmed the expression level of tPA protein from the CHO (Chinese hamster ovary) cell lines by gene transfection. Concentration of tPA expression from the six cell lines (all of CHO cells) were average 212.4 ng/ml. Reconstructed DNA to used the CHO cell were microinjected into the pronuclei of in vivo embryos The total of 2,307 zygotes were collected from 95 donors and 1,851 embryos were in 1-cell stage which were visualized the pronuclei for DNA microinjection. The concentration of linear DNA was 2.0 ng per microliter and injected into zygotes with two pronuclei on an inverted Nikon microscope equipped with narishige micromanipulator and modulation contrast optics. The 541 embryos injected with bovine $\beta$-casein promoter-tPA were transferred to 22 recipients. The 1,154 embryos injected with mouse uroplakin promoter-tPA were transferred to 51 recipients. Sixty nine offspring from 9 delivered sows were produced. We analysed the transgenes with PCR methods from 69 offsprings, but could not detect the PCR product from piglet tails DNA.

• 한국광학회지
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• 제23권3호
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• pp.108-112
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• 2012

본 논문에서는 전립선 비대증 환자 치료 시 조직 제거에 사용되는 두 파장들을 비교 연구하였다. 가시광선 영역인 532 nm는 혈액 속의 헤모글로빈에 의한 흡수율이 높은 반면, 근적외선 영역인 980 nm는 물에 의한 빛의 흡수율이 높다. 동물의 간 조직을 각각의 레이저를 이용하여 40 W 출력에서 제거하였으며, 조직 제거율을 제거 횟수, 제거 부피, 열손상 정도를 통해 검사하였다. 532 nm의 경우 높은 레이저 흡수율로 인해 조직 제거율이 980 nm 보다 4배까지 증가하였으며, 열손상은 상대적으로 약 30%까지 낮게 나타났다. 파장에 관계없이 제거 횟수가 높을수록 단위 횟수당 제거율이 낮아졌으며, 이는 레이저 빛이 거리에 따라 발산함과 동시에 제거된 조직의 입자들이 먼지 방해 현상을 일으킴으로써 효율의 저하를 일으켰음을 짐작할 수 있었다. 보다 효율적인 열적 제한 상태와 높은 흡수율로 인해 가시광선 영역의 532 nm가 근적외선 980 nm 보다 전립선 치료에 있어서 더욱 효율적인 파장임을 알 수 있었으며, 이러한 발견을 전임상 및 임상을 통해 확인할 계획이다.

• Current Optics and Photonics
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• 제2권6호
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• pp.623-628
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• 2018

Clotting properties of human blood are important clinical information to monitor for patients with platelet and coagulation disorders. Most devices used to diagnose these disorders utilize blood plasma together with tissue factors and $Ca^{{+}{+}}$ additives. In some instruments, magnetic particles were mixed with blood samples and a rotating magnetic field was applied, resulting in the rotation of magnetic particles, which was probed by impinging light. The working principle seems obvious yet had not been investigated in depth. We modeled the collective behavior of light propagating through magnetic needles, aligned in the direction of the rotating external magnetic field, with scattering light analysis software. Simulation results indicated that the scattering pattern undergoes periodic undulations with respect to the slant angle of the magnetic needles. Also provided is a means of extracting meaningful information from the scattering measurement.

• Current Optics and Photonics
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• 제2권6호
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• pp.595-605
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• 2018

The two-photon process of microscopy provides good spatial resolution and optical sectioning ability when observing quasi-static endogenous fluorescent tissue within an in vivo animal model skin. In order to extend the use of such systems, we developed a two-photon laser scanning microscopy system capable of also capturing $512{\times}512$ pixel images at 90 frames per second. This was made possible by incorporating a 72 facet polygon mirror which was mounted on a 55 kRPM motor to enhance the fast-scan axis speed in the horizontal direction. Using the enhanced temporal resolution of our high-speed two-photon laser scanning microscope, we show that rapid processes, such as fluorescently labeled erythrocytes moving in mouse blood flow at up to 1.2 mm/s, can be achieved.

• Current Optics and Photonics
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• 제7권1호
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• pp.65-72
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• 2023

The motion of organelles inside a cell is an important intrinsic indicator for assessing cell physiology and tissue viability. Dynamic contrast full-field optical coherence tomography (D-FFOCT) is a promising imaging technology that can visualize intracellular movements using the variance of temporal interference signals caused by biological motions. However, double-path interferometry in D-FFOCT can be highly vulnerable to surrounding noise, which may cause turbulence in the interference signals, contaminating the sample dynamics. Therefore, we propose a method for stabilized D-FFOCT imaging in noisy environments by using common-path interferometry in D-FFOCT. A comparative study shows that D-FFOCT with the proposed method achieves stable dynamic contrast imaging of a scattering phantom in motion that is over tenfold more noise-insensitive compared to the conventional one, and thus this imaging capability can provide cleaner motion contrast images. With the proposed approach, the intracellular dynamics of biological samples are imaged and monitored.

• Current Optics and Photonics
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• 제7권1호
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• pp.73-82
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• 2023

In this study, we demonstrate ultrahigh-resolution spectral-domain optical coherence tomography with a 200-kHz line rate using a superluminescent diode with a -3-dB bandwidth of 100 nm at 849 nm. To increase the line rate, a subset of the total number of camera pixels is used. In addition, a partial-spectrum detection method is used to obtain OCT images within an imaging depth of 2.1 mm while maintaining ultrahigh axial resolution. The partially detected spectrum has a flat-topped intensity profile, and side lobes occur after fast Fourier transformation. Consequently, we propose and apply the super-Gaussian window function as a new window function, to reduce the side lobes and obtain a result that is close to that of the axial-resolution condition with no window function applied. Upon application of the super-Gaussian window function, the result is close to the ultrahigh axial resolution of 4.2 ㎛ in air, corresponding to 3.1 ㎛ in tissue (n = 1.35).

• Current Optics and Photonics
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• 제8권2호
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• pp.192-200
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• 2024

Wheat stripe rust, caused by Puccinia striiformis, has reduced winter wheat yield globally for ages. In this work, multi-spectral Mueller matrix imaging with 37 measurements using the method of double rotatable quarter-wave plates was used to investigate wheat stripe rust. Individual Mueller matrix measurements were performed on incident monochromatic light with nine bands in the range of 430 to 690 nm. As a result, it was found that the infected area absorbed linearly polarized light and was sensitive to circularly polarized light in the spectral domain. Both linear depolarization and linear diattenuation images distinguished between wheat stripe rust and healthy tissue. The responsiveness of stripe rust to polarized light reveals the potential of using polarization imaging to detect plant diseases. This further suggests that the multi-spectral Mueller matrix imaging system provides us with an alternative approach to agricultural disease detection.

• Current Optics and Photonics
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• 제3권3호
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• pp.256-262
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• 2019

Extensive tumor resection accompanied by radiotherapy and chemotherapy is the standard of care for malignant gliomas. However, there is a significant obstacle to the complete resection of the tumor due to the difficulty of distinguishing tumor and normal brain tissue with a conventional surgical microscope. Recently, multiple studies have shown the possibility of fluorescence-guided surgery in malignant gliomas. The most used fluorescence dyes for brain tumor surgery are 5-aminolevulinic acid (5-ALA) and indocyanine green (ICG). In this paper, a new fluorescence guided operation system, which can detect both 5-ALA and ICG fluorescent images simultaneously, is presented. This operation system consists of light emitting diodes (LEDs) which emits 410 nm and 740 nm wavelengths. We have performed experiments on rats in order to verify the operation of the newly developed operation system. Oral administration and imaging were performed to observe the fluorescence of 5-ALA and ICG fluorescence in rats. When LEDs at wavelengths of 410 nm and 740 nm were irradiated on rats, 628 nm wavelength with a violet fluorescence color and 825 nm wavelength with a red fluorescence color were expressed in 5-ALA and ICG fluorescent material, respectively, thus we were able to distinguish the tumor tissues easily. Previously, due to the poor resolution of the conventional surgical microscope and the fact that the color of the vein is similar to that of the tumor, the tumor resection margin was not easy to observe, thus increasing the likelihood for cancer recurrence. However, when the tumor is observed through the fluorescence guided operation system, it is possible to easily distinguish the color with the naked eye and it can be completely removed. Therefore, it is expected that surgical removal of cancerous tumors will be possible and surgical applications and surgical microscopes for cancer tumor removal surgery will be promising in the future.