• KSBB Journal
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• v.14 no.1
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• pp.1-8
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• 1999

It confirmed the facilitated diffusion of $Na^+$ of frog bladder membrane which is a tissue membrane. The mechanism was explained in $Na^+$ channel model and its referred to the $Na^+$ channel obstruction ingredient which was contained in the reference to the $Na^+$ channel obstruction ingredient and son on, e.g., seaweed, shellfish, pufferfish, phytoplankton and chinese drug. Also, it introduces the result which studied from the barrier point of the application of the tissue biosensor to the trade friction on Korea or Japan pufferfish and the marine environment in the one with high dependance. It was possible for the poison quantity of small amount pufferfish toxin (TTX), paralytic shellfish poisoning (PSP) to be measured and also to measure poison quantity in the cultivation poisonous toxin phytoplankton individual. In future, as for this tissue biosensor, it expects that it is possible to contribute widely until environment watch and also monitoring to the scene.

• Toxicological Research
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• v.31 no.2
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• pp.213-217
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• 2015

A voltammetric toxic metal of cadmium detection was studied using a fluorine doped graphite pencil electrode (FPE) in a seawater electrolyte. In this study, square wave (SW) stripping and chronoamerometry were used for determination of Cd(II) in seawater. Affordable pencils and an auxiliary electrode were used as reference. All experiments in this study could be performed at reasonable cost by using graphite pencil. The application was performed on the tissue of contaminated soil earthworm. The results show that the method can be applicable for vegetables and in vivo fluid or medicinal diagnosis.

• KSBB Journal
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• v.9 no.3
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• pp.319-324
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• 1994

A biosensor for the measurement of malate has been constructed by the sodium-alginate immobilized bundle sheath cell tissue of corn leaf containing malate dehydrogenase (decarboxylating) (EC 1. 1. 1. 40) on the CO2 gas-sensing electrode. The proposed tissue sensor had the linear in the range of malate concentration $5.5{\times}10^{-5}M∼2.5{\times}10^{-2}M$ with a slope of 53.5 mV/decade in 0.02M Tris-HCl buffer solution at optimum pH 8.0, and $25^{\circ}C$. A response time was 16∼18min. The present L-malate sensing tissue sensor is stable for more than one week. At pH 7.4, Km value was $0.6{\times}10^{-5}M$. The various kinds of salt did not effect the signal of malate tissue biosensor as the inhibitor. We can measure the malate by the CO2 electrode at the pH=8.0. Thus, the proposed tissue sensor will be useful for the measurement of malate.

• Applied Chemistry for Engineering
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• v.25 no.1
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• pp.107-112
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• 2014

Using a chlorosulphonated polyethylene rubber solution for a binder of graphite powder and ferrocene for a mediator, a rose leaf tissue-embedded biosensor was built. Linearity on the Hanes-Woolf plot showed the reduction of the substrate was attained through the catalytic power of the rose peroxidase in the experimental range of electrode potential. Furthermore, 10 or more electrochemical parameters demonstrated that the electrode exerts its sensing ability quantitatively. The foregoing gave the full conviction that rose tissue can be used in place of the currently marketed enzyme for the practical use of enzyme electrode.

• Analytical Science and Technology
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• v.6 no.3
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• pp.313-318
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• 1993

The rose petal tissue biosensor has been constructed by immobilizing New carina rose tissue. Optimum conditions for the determination of urea were investigated using this sensor. Selectivity and life time of this sensor were also obtained. As a result, the biosensor showed the optimum response characteristics in 0.20M phosphate buffer solution at pH 8.0, $37^{\circ}C$ and 50mg of tissue amounts. This sensor was linear from $9.0{\times}10^{-5}$ to $4.0{\times}10^{-3}M$ urea with a slope of 42mV/decade. The limit of detection and response time are $7.0{\times}10^{-5}M$ and 17~19 min.

• Journal of the Korean Chemical Society
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• v.51 no.3
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• pp.291-297
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• 2007

• Journal of the Korean Chemical Society
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• v.48 no.6
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• pp.654-658
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• 2004

• KSBB Journal
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• v.13 no.1
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• pp.71-76
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• 1998

Tissue biosensor for mearsuring sodium channel blockers, such tetrodotoxin(TTX), saxitoxin (STX) and paralytic shellfish poisoning(PSP) consisted of frog bladder membrane, and $Na^{+}$ electrode. The proposed biosensor was applied to determine Chinese drug and dry or wet Porphyra yezonesis $Na^{+}$ channel blockers below the detection limit of the standard mouse bio-assay while the observed detection limit didn't cause human poisoning. The proposed biosensor system may be used for future $Na^{+}$ channel blockers monitoring within the marine environment.

• Journal of the Korean Chemical Society
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• v.41 no.7
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• pp.343-350
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• 1997

The response characteristics of a new biosensor, which was developed by co-immobilisation of chicken liver tissue and ferrocene in a carbon paste matrix for the amperometric determination of hydrogen peroxide, was evaluated. In the range of electrode potential observed ( - 0.5∼ + 0.05 V vs Ag/AgCl), the bioelectrode showed the response time ($t_{95%}$) as low as 13 sec and the detection limit of $5.1{\times}10^{-5}$ M, and exhibited a good selectivity in the case of the addition of the possible interferents tested. Also it offered a high biocatalytic stability and opened up the possibility for the construction of a mediatorless biosensor.

• Analytical Science and Technology
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• v.19 no.1
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• pp.58-64
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• 2006

A mugwort-tissue-based modified carbon paste electrode was constructed for the amperometric detection of hydrogen peroxide and its electrochemical properties are described. Especially the amperometric signal was very stable and bigger than any other enzyme electrode studied in this lab. The effect of tissue composition on the response was linear within the wide range of experiment and the linearity of Lineweaver-Burk plot showed that the sensing process of the biosensor is by enzymatic catalysis. And pH dependent current profile connoted that two isozymes are active in this system.