• 치과방사선
• /
• 제24권2호
• /
• pp.263-274
• /
• 1994

The purpose of this study was to investigate the remodeling process of the streptozotocin-induced diabetic rat's resected condyle. This experiment was performed with male Sprague-Dawly strain rats weighing approximately 250 gm, which were rendered diabetic by an intravenous injection of streptozotocin(70㎎/㎏ body weight). After condylectomy, experimental rats were serially terminated on the 1st week, the 2nd week, the 3rd week, and the 4th week. The following termination, the mandibles were dissected out to make specimens. Each mandibular condyle was radiographed with Hitex HA-80(Hitex Co., Ltd. Japan). In addition to radiographic observation, the mandibular condyles, further decalcified and embedded in paraffin, were sectioned and stained with Hematoxylin and Eosin, Toluidine blue and Masson's trichrome. They were observed with a light microscope and a polarizing microscope. The results were as follows. 1. Soft X-ray radiograms revealed proliferation of bone after 1 week in both groups. Irregularly repaired bones and dense trabeculae were clearly observed in experimental group. 2. The resected condyles were repaired by intramembraneous and endochondral bone formation in both groups. 3. Bone tissue repair was initiated from the adjacent margin of resected bone, and cartilaginous tissues were observed at the top of repaired bone in both groups. 4. The number of osteoblasts of experimental group was small, compared with control group. Each osteoblast was small and flat. The thin trabeculae were irregularly formed. 5. Collagens of bone were gradually matured in both groups, but the degree of maturation was lower in experimental group. 6. Fibrous tissues covered the upper parts of repaired bone were densely arranged in the both groups. Conclusively, atrophied osteoblasts, immature collagen of bone, and thin and irregular trabeculae which were characterized in the diabetes experimental group showed diabetes disturbed osteoblastic function and caused disturbance of remodeling process of bone.

• 한국발생생물학회지:발생과생식
• /
• 제13권3호
• /
• pp.173-181
• /
• 2009

One of the most extensively studied populations of multipotent adult stem cells are mesenchymal stem cells (MSCs). MSCs derived from the human umbilical cord vein (HUC-MSCs) are morphologically and immunophenotypically similar to MSCs isolated from bone marrow. HUC-MSCs are multipotent stem cells, differ from hematopoietic stem cells and can be differentiated into neural cells. Since neural tissue has limited intrinsic capacity of repair after injury, the identification of alternate sources of neural stem cells has broad clinical potential. We isolated mesenchymal-like stem cells from the human umbilical cord vein, and studied transdifferentiation-promoting conditions in neural cells. Dopaminergic neuronal differentiation of HUC-MSCs was also studied. Neural differentiation was induced by adding bFGF, EGF, dimethyl sulfoxide (DMSO) and butylated hydroxyanisole (BHA) in N2 medium and N2 supplement. The immunoreactive cells for $\beta$-tubulin III, a neuron-specific marker, GFAP, an astrocyte marker, or Gal-C, an oligodendrocyte marker, were found. HUC-MSCs treated with bFGF, SHH and FGF8 were differentiated into dopaminergic neurons that were immunopositive for tyrosine hydroxylase (TH) antibody. HUC-MSCs treated with DMSO and BHA rapidly showed the morphology of multipolar neurons. Both immunocytochemistry and RT-PCR analysis indicated that the expression of a number of neural markers including NeuroD1, $\beta$-tubulin III, GFAP and nestin was markedly elevated during this acute differentiation. While the stem cell markers such as SCF, C-kit, and Stat-3 were not expressed after neural differentiation, we confirmed the differentiation of dopaminergic neurons by TH/$\beta$-tubulin III positive cells. In conclusion, HUC-MSCs can be differentiated into dopaminergic neurons and these findings suggest that HUC-MSCs are alternative cell source of therapeutic treatment for neurodegenerative diseases.

• Reproductive and Developmental Biology
• /
• 제36권1호
• /
• pp.33-37
• /
• 2012

Differential capacity of the parthenogenetic embryonic stem cells (PESCs) is still under controversy and the mechanisms of its neural induction are yet poorly understood. Here we demonstrated neural lineage induction of PESCs by addition of insulin-like growth factor-2 (Igf2), which is an important factor for embryo organ development and a paternally expressed imprinting gene. Murine PESCs were aggregated to embryoid bodies (EBs) by suspension culture under the leukemia inhibitory factor-free condition for 4 days. To test the effect of exogenous Igf2, 30 ng/ml of Igf2 was supplemented to EBs induction medium. Then neural induction was carried out with serum-free medium containing insulin, transferrin, selenium, and fibronectin complex (ITSFn) for 12 days. Normal murine embryonic stem cells derived from fertilized embryos (ESCs) were used as the control group. Neural potential of differentiated PESCs and ESCs were analyzed by immunofluorescent labeling and real-time PCR assay (Nestin, neural progenitor marker; Tuj1, neuronal cell marker; GFAP, glial cell marker). The differentiated cells from both ESC and PESC showed heterogeneous population of Nestin, Tuj1, and GFAP positive cells. In terms of the level of gene expression, PESC showed 4 times higher level of GFAP expression than ESCs. After exposure to Igf2, the expression level of GFAP decreased both in derivatives of PESCs and ESCs. Interestingly, the expression level of $Tuj1$ increased only in ESCs, not in PESCs. The results show that IGF2 is a positive effector for suppressing over-expressed glial differentiation during neural induction of PESCs and for promoting neuronal differentiation of ESCs, while exogenous Igf2 could not accelerate the neuronal differentiation of PESCs. Although exogenous Igf2 promotes neuronal differentiation of normal ESCs, expression of endogenous $Igf2$ may be critical for initiating neuronal differentiation of pluripotent stem cells. The findings may contribute to understanding of the relationship between imprinting mechanism and neural differentiation and its application to neural tissue repair in the future.

• 대한기관식도과학회지
• /
• 제8권1호
• /
• pp.29-34
• /
• 2002

Background and Objectives : Sulfur dioxide gas is one of the major airborne Pollutants noxious to human in industrialized countries. The most vulnerable areas in the human respiratory system were the trachea and main bronchi and a gradient of decreasing damage was observed in the peripheral tracheobronchial tree. Induced functional alteration was increased mucosal permeability, and morphological changes were epithelial sloughing, intracellular edema, mitochondrial swelling, widened intercellular spaces, and ciliary cytoplamic extrusions. The laminins are a family of extracellular matrix glycoproteins localized in the basement membrane. Their primary role is cell-matrix attachment, but many additional biologic activities, including Promoting cell growth and migration, tumor growth and metastasis, wound repair, and graft survival, have been demonstrated. Materials and Methods : Histologic changes and expression of laminin in tracheal mucosa sacrificed at 1 day, 2 day, 3 day, 1 week, 2 weeks, and 3 weeks after continued SO2 exposure of 250 ppm for 30 minutes a day(to 7week) were studied in rats. In this study, mild immune reaction for laminin was noted at the apical cytoplasm of epithelial cells and basement membrane one day after a 7 week $SO_2$ exposure. The cilia and nucleoi of epithelial cells were normal and no immune reaction was noted in Goblet cells. The lamina propria of the tracheal tissue was infiltrated by monocytes and lymphocytes. Results : At 24 hours after exposure, all tracheal cells except Goblet cells revealed a mild immune reaction for laminin. No immune reactions were noted in the basement membrane. At 72 hours after exposure, mild or moderate immune reactions for laminin was seen in the tracheal cell cytoplasm. Irregular faint immune reaction for laminin was noted in the basement membrane. At 1 week after exposure, strong immune reaction for laminin was detected over all tracheal cells, and the basement membrane was seen clearly. At 2~3 weeks after exposure, strong immune reaction for laminin was seen in all tracheal epithelial cells except Goblet cells and a mild immune reaction was partly revealed in the basement membrane. Conclusion : Our study suggests that 502 produces histologic damage on the tracheal mucosa. Longer duration after exposure of $SO_2$ makes more progressive healing on the tracheal mucosa and increased immunoreactivity for laminin.

• 한국산림과학회지
• /
• 제86권1호
• /
• pp.80-86
• /
• 1997

잡종(雜種)포플러가 어떻게 오존 stress에서 피해(被害)를 최소화하는가를 알아보기 위해서 생장(生長), 광합성율(光合成率), 그리고 초기(初期) Rubisco의 활성(活性)을 조사(調査)하였다. 이 연구(硏究)에서는 Populus trichocarpa${\times}$P. deltoides 삽목묘(揷木苗)를 재료(材料)로 사용(使用)하였다. 오존처리농도(處理濃度)는 90-115 ppb으로, 하루에 6-9시간씩, 126일 동안 처리하였다. 이 연구에서는, 오존에 저항성(抵抗性)을 가지는 포플러는 광합성율(光合成率)과 Rubisco의 활성(活性)을 증가(增加)시켜서 그 물질(物質) 생산량을 유지(維持)하여 stress를 보상(補償)한다는 가설(假說)을 검정(檢定)하였다. 생장(生長), 물질생산량(物質生産量), 광합성율(光合成率), 그리고 초기(初期) Rubisco 활성(活性)이 일반적으로 오존에 의해서 감소(減少)하였다. 뿌리의 생장량은 지상부(地上部)의 피해(被害)를 보상하기 위한 탄소(炭素)의 불균등(不均等)한 분배(分配)때문에, 가장 피해가 심한 부위(部位)였다. 저항성을 가지는 포플러는 오존에 대해서 수체내(樹體內)의 광합성율(光合成率)과 초기 Rubisco 활성을 높여 생산량(生産量)을 유지(維持)하는 생물적(生物的)인 보상(補償)을 하는 것으로 밝혀졌다.

• Journal of Periodontal and Implant Science
• /
• 제32권1호
• /
• pp.213-224
• /
• 2002

The ultimate goal of periodontal disease therapy is to promote the regeneration of lost periodontal tissue, there have been many attempts to develop a method to achieve this goal, but none of them was completely successful. The purpose of this study was to compare the effects of treatment using BBP(R) with control treated by only modified Widman flap. 22 intrabony defects from 12 patients with chronic periodontitis were used for this study, 10 sites of them were treated with BBP(R) as experimental group and 12 site were treated by only modified Widman flap as control group. Clinical parameters including probing depth, gingival recession, bone probing depth and loss of attachment were recorded at 6 months later, and the significance of the changes was statistically analyzed. The results are as follows : 1. Probing depth of control(${\triangle}2.7{\pm}1.3mm$) and experimental group(${\triangle}3.6{\pm}1.8mm$) weres reduced with statistically significance(P<0.05), but this changes were not different between the two experiment, control group with statistically significance. 2. Gingival recession showed statistically significant increase in control group(${\triangle}2.1{\pm}1.2mm$)(P<0.05), but not in experimental group(${\triangle}0.5{\pm}0.7mm$), and this changes were different between the two experiment, control group with statistically significance(P<0.05). 3. Bone probing depth showed statistically significant decrease in experimental group(${\triangle}2.9{\pm}1.0mm$)(P<0.05), but not in control group(${\triangle}1.1{\pm}1.4mm$), and this changes were different between the two experiment, control group with statistically significance(P<0.05). 4. Loss of attachment showed statistically significant decrease in experimental group(${\triangle}3.1{\pm}1.7mm$), but not in control group(${\triangle}0.6{\pm}1.2mm$), and this changes were different between the two experiment, control group with statistically significance(P<0.05) On the basis of these results, treatment using BBP(R) improves the probing depth, bone probing depth and loss of attachment in intrabony defects.

• 대한소아치과학회지
• /
• 제27권3호
• /
• pp.388-393
• /
• 2000

순 구개열(cleft lip and palate)은 구강악안면 영역에서 가장 빈번하게 발생하는 선천성 기형 중 하나로 유전적, 환경적 요인에 의해 발생된다. 순 구개열 환자는 입술, 코 등의 기형을 보이며 치과적 문제점으로, 이환측 측절치는 결손되어 있거나 과잉치가 존재하고 형태적 이상을 보이는 경우가 많고, 맹출하지 못하거나 이소맹출을 하게된다. 이른 시기에 수행된 수술의 반흔조직(scar tissue)으로 인하여 상악골 성장의 장애가 발생되어 흔히 골격성 III급 부정교합이 야기되기 쉬우며, 섭식장애와 발음장애를 보이기도 한다. 이러한 복잡한 문제로 인하여 여러 분야의 전문가들로부터 다양한 관리가 필요하다. 순/구개열(cleft lip and alveolus only)은 악안면 영역에 제한적으로 영향을 미친다. 비이환측은 전방으로 회전되어 수평피개가 증가되고, 이환측은 내측으로 회전되어 견치부에서 end to end 관계, 혹은 반대교합을 보인다. 순/구개열 수술은 이와 같은 문제점 등을 개선시키기 위한 것이며, 상악골의 전후방적 성장에는 큰 영향을 미치지 않는 것으로 보고되고 있다. 본 증례는 좌측에 순/구개열을 가진 5세 3개월의 여아로 생후 3개월 및 3세에 두 번의 구순열 봉합 수술을 받았으며, 상악 좌측 유측절치와 영구측절치가 결손되어 있었고, 다발성 우식증 및 비구누공(oroantral fistula)이 존재하였으며, 골격성 3급 부정교합을 보였다. 그러나 본 증례가 지니고 있는 골격성 III급 부정교합은 구순열 수술후의 반흔조직에 의한 것은 아닌 것으로 판단되었으며, 환자의 기능과 심미성을 회복하기 위하여 혼합치열기 동안 공간관리(space supervision) 및 악기능 장치 (functional regulator)를 이용한 골격성 부정교합을 치료하였으며, 영구치열기 동안 고정성 교정치료를 시행한 후 보철 치료를 시행하여 비구누공을 key and keyway attachment와 Konus crown을 이용한 가철성 obturator로 폐쇄하여 양호한 결과를 보였다.

• Journal of Periodontal and Implant Science
• /
• 제37권4호
• /
• pp.655-669
• /
• 2007

Reactive oxygen species (ROS) have been implicated in the pathogenesis of various diseases. And vitamin C has shown a protective effect for the tissues. The aim of this study was to evaluate the effects of $H_2O_2$ and ascorbic acid on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase (TIMP: TIMP-1, TIMP-2), Type 1 collagen, fibronectin, and PDLs22 level in human periodontal ligament fibroblasts (hPDLF) via reverse transcription-polymerase chain reaction (RT-PCR). hPDLF was obtained from a healthy periodontium and cultured in Dulbecco's modified Eagles's medium plus 10% fetal bone serum. The concentration of ascorbic acid in hPDLF was $50{\mu}g/ml$, and that of $H_2O_2$ in hPDLF was 0.03% and 0.00003%. Ascorbic acid only, $H_2O_2$ only and mixture of ascorbic acid and $H_2O_2$ were applied with hPDLF for 1-, 3-, and 30-min. respectively. The gene expression of MMP-1-, TIMP-1-, TIMP-2-, Type 1 collagen-, fibronectin-, and PDLs22-mRNA in hPDLF was analysed via RT-PCR. The results were as follows; 1. hPDLF in response to 30-min. incubation with 0.03% $H_2O_2$ did not show any gene expression. 2. In all the experimental groups, the gene expression of fibronectin mRNA showed the decreased tendency compared to control. 3. In all the experimental groups, the gene expression of TIMP-1 mRNA showed the tendency similar to control. 4. hPDLF in response to 30-min. incubation with 0.03% $H_2O_2$ and ascorbic acid increased mRNA induction for MMP-1. 5. In all the experimental groups, hPDLF increased mRNA induction for PDLs22, collagen type 1, and TIMP-2 compared to control. Within the limited experiments, $H_2O_2$ and ascorbic acid increased mRNA induction for PDLs22, collagen type 1, TIMP-2 in hPDLF. More research will be needed in order to confirm the relative importance of the different roles of ROS and antioxidants in hPDLF from a periodontal regeneration or repair standpoint.

• Journal of Microbiology and Biotechnology
• /
• 제26권6호
• /
• pp.1140-1147
• /
• 2016

The plasma and serum of Crocodylus siamensis have previously been reported to exhibit potent antimicrobial, antioxidant, and anti-inflammatory activities. During wound healing, these biological properties play a crucial role for supporting the formation of new tissue around the injured skin in the recovery process. Thus, this study aimed to evaluate the wound healing properties of C. siamensis plasma and serum. The collected data demonstrate that crocodile plasma and serum were able to activate in vitro proliferation and migration of HaCaT, a human keratinocyte cell line, which represents an essential phase in the wound healing process. With respect to investigating cell migration, a scratch wound experiment was performed which revealed the ability of plasma and serum to decrease the gap of wounds in a dose-dependent manner. Consistent with the in vitro results, remarkably enhanced wound repair was also observed in a mouse excisional skin wound model after treatment with plasma or serum. The effects of C. siamensis plasma and serum on wound healing were further elucidated by treating wound infections by Staphylococcus aureus ATCC 25923 on mice skin coupled with a histological method. The results indicate that crocodile plasma and serum promote the prevention of wound infection and boost the re-epithelialization necessary for the formation of new skin. Therefore, this work represents the first study to demonstrate the efficiency of C. siamensis plasma and serum with respect to their wound healing properties and strongly supports the utilization of C. siamensis plasma and serum as therapeutic products for injured skin treatment.

• 한국임상수의학회지
• /
• 제31권6호
• /
• pp.523-526
• /
• 2014

세마리의 개가 골절을 주증으로 내원하였다. 증례 1(1.8 kg의 5개월령 암컷 몰티즈)과 증례 2(3.0 kg의 6개월령 암컷 푸들)는 좌측 후지의 체중부중을 하지 않는 파행을, 증례 3(3.3 kg의 나이를 알 수 없는 암컷 잡종견)은 좌측 전지의 체중부중을 하지 않는 파행을 보였다. 정형외과 검사시 손상받은 부위의 통증, 염발음, 촉진상의 불안정성과 광범위한 연부조직 부종을 보였으며 신경학적 이상소견은 관찰되지 않았다. 방사선 검사상 증례 1와 2에서 좌측 근위 경골 골간단과 비골의 골절이, 증례 3에서는 좌측 근위 요골 골간단과 척골의 골절이 관찰되었다. 모든 증례는 골절편이 짧은 관절주위 폐쇄형 골절이었다. 술 중에는 투시장치를 이용해 2개 또는 3개의 원형고정장치의 적절한 위치에 삽입하였다. 술 후 방사선 사진에서 골절부위의 적절한 배열과 위치를 확인하였다. 방사선학적으로 5주에서 14주 사이에서 골유합이 확인되었고 임플란트의 고정 실패나 핀 주변 감염은 나타나지 않았다. 기능상 결과는 모든 증례에서 매우 좋은 것으로 나타났다. 따라서, 원형외고정장치는 뼈판이나 외고정장치를 사용할 수 없는 짧은 골절편을 가진 관절주의의 골절정복에 성공적으로 이용할 수 있을 거라 사료된다.