Browse > Article
http://dx.doi.org/10.4014/jmb.1601.01054

In Vitro and in Vivo Wound Healing Properties of Plasma and Serum from Crocodylus siamensis Blood  

Jangpromma, Nisachon (Office of the Dean, Faculty of Science, Khon Kaen University)
Preecharram, Sutthidech (Department of General Science, Faculty of Science and Engineering, Kasetsart University)
Srilert, Thanawan (Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University)
Maijaroen, Surachai (Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University)
Mahakunakorn, Pramote (Department of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University)
Nualkaew, Natsajee (Department of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University)
Daduang, Sakda (Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University)
Klaynongsruang, Sompong (Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.6, 2016 , pp. 1140-1147 More about this Journal
Abstract
The plasma and serum of Crocodylus siamensis have previously been reported to exhibit potent antimicrobial, antioxidant, and anti-inflammatory activities. During wound healing, these biological properties play a crucial role for supporting the formation of new tissue around the injured skin in the recovery process. Thus, this study aimed to evaluate the wound healing properties of C. siamensis plasma and serum. The collected data demonstrate that crocodile plasma and serum were able to activate in vitro proliferation and migration of HaCaT, a human keratinocyte cell line, which represents an essential phase in the wound healing process. With respect to investigating cell migration, a scratch wound experiment was performed which revealed the ability of plasma and serum to decrease the gap of wounds in a dose-dependent manner. Consistent with the in vitro results, remarkably enhanced wound repair was also observed in a mouse excisional skin wound model after treatment with plasma or serum. The effects of C. siamensis plasma and serum on wound healing were further elucidated by treating wound infections by Staphylococcus aureus ATCC 25923 on mice skin coupled with a histological method. The results indicate that crocodile plasma and serum promote the prevention of wound infection and boost the re-epithelialization necessary for the formation of new skin. Therefore, this work represents the first study to demonstrate the efficiency of C. siamensis plasma and serum with respect to their wound healing properties and strongly supports the utilization of C. siamensis plasma and serum as therapeutic products for injured skin treatment.
Keywords
Wound healing; wound infection; scratch assay; plasma; serum; crocodile blood;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Preecharram S, Jearranaiprepame P, Daduang S, Temsiripong Y, Somdee T, Fukamizo T, et al. 2010. Isolation and characterisation of crocosin, an antibacterial compound from crocodile (Crocodylus siamensis) plasma. Anim. Sci. J. 81: 393-401.   DOI
2 Ranzato E, Patrone M, Mazzucco L, Burlando B. 2008. Platelet lysate stimulates wound repair of HaCaT keratinocytes. Br. J. Dermatol. 159: 537-545.
3 Sun DP, Yeh CH, So E, Wang LY, Wei TS, Chang MS, Hsing CH. 2013. Interleukin (IL)-19 promoted skin wound healing by increasing fibroblast keratinocyte growth factor expression. Cytokine 62: 360-368.   DOI
4 Tang J, Liu H, Gao C, Mu L, Yang S, Rong M, et al. 2014. A small peptide with potential ability to promote wound healing. PLoS One 9: e92082.   DOI
5 van Hoek ML. 2014. Antimicrobial peptides in reptiles. Pharmaceuticals 7: 723-753.   DOI
6 Yang X, Wang J, Guo SL, Fan KJ, Li J, Wang YL, et al. 2011. miR-21 promotes keratinocyte migration and re-epithelialization during wound healing Int. J. Biol. Sci. 7: 685-690   DOI
7 Zhang Y, Bai X, Wang Y, Li N, Li X, Han F, et al. 2014. Rolefor heat shock protein 90a in the proliferation and migration of HaCaT cells and in the deep second-degree burn wound healing in mice. PLoS One 9: e103723.   DOI
8 Hamblin MR, Zahra T, Contag CH, McManus AT, Hasan T. 2003. Optical monitoring and treatment of potentially lethal wound infections in vivo. J. Infect. Dis. 187: 1717-1725.   DOI
9 Carretero M, Escámez MJ, García M, Duarte B, Holguín A, Retamosa L, et al. 2008. In vitro and in vivo wound healing-promoting activities of human cathelicidin LL-37. J. Invest. Dermatol. 128: 223-236.   DOI
10 Carter CA, Jolly DG, Worden CES, Hendren DG, Kane CJ. 2003. Platelet-rich plasma gel promotes differentiation and regeneration during equine wound healing. Exp. Mol. Pathol. 74: 244-255.   DOI
11 Demirci S, Doğan A, Demirci Y, Şahin F. 2014. In vitro wound healing activity of methanol extract of Verbascum speciosum. Int. J. Appl. Res. Nat. Prod. 7: 37-44.
12 Jorge MP, Madjarof C, Gois Ruiz AL, Fernandes AT, Ferreira Rodrigues RA, de Oliveira Sousa IM, et al. 2008. Evaluation of wound healing properties of Arrabidaea chica Verlot extract. J. Ethnopharmacol. 13: 361-366.   DOI
13 Kommanee J, Phosri S, Daduang S, Temsiripong Y, Dhiravisit A, Thammasirirak S. 2014. Comparisons of anti-inflammatory activity of crocodile (Crocodylus siamensis) blood extract. Chiang Mai J. Sci. 41: 627-634.
14 Merchant ME, Thibodeaux D, Loubser K, Elsey RM. 2004. Amoebacidal effects of serum from the American alligator (Alligator mississippiensis). J. Parasitol. 90: 1480-1483.   DOI
15 Kommanee J, Preecharram S, Daduang S, Temsiripong Y, Dhiravisit A, Yamada Y, Thammasirirak S. 2012. Antibacterial activity of plasma from crocodile (Crocodylus siamensis) against pathogenic bacteria. Ann. Clin. Microbiol. Antimicrob. 11: 22.   DOI
16 Kurahashi T, Fujii J. 2015. Roles of antioxidative enzymes in wound healing. J. Dev. Biol. 3: 57-70.   DOI
17 Leu JG, Chen SA, Chen HM, Wu WM, Hung CF, Yao YD, et al. 2012. The effects of gold nanoparticles in wound healing with antioxidant epigallocatechin gallate and α-lipoic acid. Nanomedicine 8: 767-775.   DOI
18 Phosri S, Mahakunakorn P, Lueangsakulthai J, Jangpromma N, Swatsitang P, Daduang S, et al. 2014. An investigation of antioxidant and anti-inflammatory activities from blood components of crocodile (Crocodylus siamensis). Protein J. 33: 484-492.   DOI
19 Merchant ME, Roche C, Elsey RM, Prudhomme J. 2003. Antibacterial properties of serum from the American alligator (Alligator mississippiensis). Comp. Biochem. Physiol. B Biochem. Mol. Biol. 136: 505-513.   DOI
20 Preecharram S, Daduang S, Bunyatratchata W, Araki T, Thammasirirak S. 2008. Antibacterial activity from Siamese crocodile (Crocodylus siamensis) serum. Afr. J. Biotechnol. 7: 3121-3128.
21 Merchant ME, Roche CM, Thibodeaux D, Elsey RM. 2005. Identification of alternative pathway serum complement activity in the blood of the American alligator (Alligator mississippiensis). Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 141: 281-288.   DOI
22 Buthelezi S, Southway C, Govinden U, Bodenstein J, du Toit K. 2012. An investigation of the antimicrobial and anti-inflammatory activities of crocodile oil. J. Ethnopharmacol. 143: 325-230.   DOI
23 Arunachalam K, Parimelazhagan T. 2013. Anti-inflammatory, wound healing and in-vivo antioxidant properties of the leaves of Ficus amplissima Smith. J. Ethnopharmacol. 145: 139-145.   DOI