• 제목/요약/키워드: Tip Resistance

검색결과 293건 처리시간 0.024초

Agrobacterium을 이용한 형질전환에서 sonication과 vir 유전자들의 효과 (Effect of Sonication and vir Genes on Transient Gene Expression in Agrobacterium-Mediated Transformation)

  • 이병무
    • 생명과학회지
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    • 제11권4호
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    • pp.316-320
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    • 2001
  • Sonication tremendously improves the efficiency of Agrobacterium infection by introducing small and uniform fissures and channels throughout the targeted tissue. Using shoot tips of cotton as explants, the effect of sonication treatment and virulence genes in Agrobacterium tumefaciens on transformation efficiency was investigated. The pat gene which encodes resistance to the herbicide, glufosinate, was used as a selectable marker. Transformation efficiency was evaluated on th basis of survival rates of cocultivated shoot tips on selection medium containing 2.5 mg/l gulfosinate-ammonium(ppt) adn 25. mg/l Clavamax. Sonication from 5 to 15 second has a positive effect on shoop tip survival. However, whil virE as well as virG or vir GN54D showed an enhancement in transformation efficiency, virE,. virG resulted in the most significant enhancement. Overall, the combination of additional virG/virE gene and sonication treatment resulted in the most significant increase in transformation efficiency.

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점용접 시 압흔 깊이 감소를 위한 절연팁 개발에 관한 연구 (A Study on the Development of Insulated Electrode Tip for Spot Welding to Reduce Indentation)

  • 서승일;장상길
    • Journal of Welding and Joining
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    • 제21권1호
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    • pp.42-47
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    • 2003
  • Stainless rolling stocks are usually fabricated by spot welding process without painting. Indentation on the surface of the car body after spot welding injures the beauty of the stainless rolling stocks. In this study, insulated electrode tips to reduce the indentation are developed and applied to the actual spot welding works. The developed tips are composed of head, neck, hole for cooling water, body and resistance material. They provide large surface contact area with the base materials during spot welding and enhance the current density by necking. Experimental results using the developed tips show that small indentation and sufficient tensile shear strength is produced due to large contact area and enhanced current density.

室溫時效 및 變形時效가 微小 疲勞크랙의 開口變位에 미치는 影響 (Influence of Room Temperature and Strain Aging on the COD for a Small Fatigue Crack)

  • 김민건
    • 대한기계학회논문집
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    • 제19권2호
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    • pp.402-407
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    • 1995
  • The effects of room temperature and strain aging treatment are discussed on the critical condition for the onset of growth of non-propagating cracks on 0.15% C low carbon steel, with special emphasis on the length of the critical non-propagating crack and on the crack opening displacement(COD) at the crack tip. It is found from the experimental analysis that room temperature and strain aging of a fatigue pre-cracked specimen introduced the closure of a crack tip of the pre-crack and the reduction of crack opening displacement at the wake of crack, together with an improvement in crack growth resistance of the microstructure. This may cause an increase in the endurance limit of the specimen, through the enhancement of effective stress for the onset of growth of the critical non-propagating crack.

열원 형태에 의한 전자재료의 접합성에 관한 연구 I (A Study on Bondability of Electronic Materials by Different Heat Sources)

  • 신영의;양협;김경섭
    • Journal of Welding and Joining
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    • 제12권4호
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    • pp.110-116
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    • 1994
  • This paper has been researched bondability of electronics devices, such as lead frame and the thick film of Ag/Pd on an alumina substrate by different heat sources. To obtain the bonds with high quality, it is very important to consist of different materials. Therefore, this paper clarifies not only heat mechanism of micro parallel gap resistance bonding method and pulse heat tip bonding method but also investigates selection of heat sources with micro-electronic materials for bonding. Finally, it is realized fluxless bonding process with filler metal such as plating layers.

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유한요소법에 의한 플라스틱 파이프의 저속균열성장 저항성 시험편 균열선단 모드 I 응력확대계수 계산 (Computation of Crack Tip Mode I Stress Intensity Factor of a Specimen for Measuring Slow Crack Growth Resistance of Plastic Pipes Using Finite-Element Method)

  • 최선웅;박영주;서영성
    • 대한기계학회논문집A
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    • 제29권9호
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    • pp.1225-1234
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    • 2005
  • Mode I stress intensity factor $(K_I)$ of Notched Ring Test(NRT) specimen for measuring slow crack growth resistance was found using finite-element method. The theoretical $K_I$ value of NRT was not available in any references and could not be solved analytically. At first, in order to verify the accuracy of the finite-element approach, published $K_I$ values of several cracks were calculated and compared with finite-element results. The results were in good agreement within inherent errors of theoretical $K_I$. Finally the mode I stress intensity factor of NRT was found using 2- and 3-dimensional finite-element methods and expressed as a function of the applied load. This enabled direct comparison of resistance to slow crack growth between NRT and Notched Pipe Test(NPT), which employ different loading regime.

순차적 설계기법에 의한 DWT 75,000 정유운반선의 선형설계 (Stepwise Hull Form Design of DWT 75,000 Product Oil Carrier)

  • 박연석;박세라;정요한;최정규;유재훈
    • 대한조선학회논문집
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    • 제53권6호
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    • pp.456-464
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    • 2016
  • To design the modified hull form with relatively unfavorable dimensions and constraints than the parent ship the stepwise design was applied. In each design step the resistance characteristics was estimated by numerical calculations using CFD programs as Wavis 1.4, Wavis 2.1 and Fluent 12.1. The wave profiles along hull surface by potential flow calculations were investigated to improve wave resistance by modifying the bow shapes. To improve the stern shapes with a point of viscous form resistance the pressure distributions on hull surface and the limiting streamlines are investigated by viscous flow calculations. The design objectives such as shortening the LBP, enlarging the propeller tip clearance, moving forward of the LCB location and increasing the displacement were applied by stepwise to develop the new hull form of DWT 75,000 product oil carrier. Finally a new hull form was developed without the resistance performance loss compared with the parent ship.

청도군이서면에 발생한 수도심고병선충의 전염경로와 온탕처리방제시험 (Studies on the appearance, distribution varietal resistance and disinfection of white tip Aphelenchoides besseyi Christie of rice in Korea)

  • 전우방
    • 한국작물학회지
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    • 제10권
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    • pp.51-56
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    • 1971
  • 청도군이서면과 김제군죽산면에서 발생된 수도심고선충을 분리하여 그 병실과 병원선충의 형태를 조사하고 그 전염경로와 온탕처리방제에 관한 약간의 실험을 실시하였는데 그 지과를 요약하면 다음과 같다. 1. 발병되었던 포장에서는 매년발병된다. 즉 포장전염을 한다. 2. 발병되었던 벼 종자는 그 종자를 통해서 전염한다. 3. 관개수를 통해서 전염한다. 4. 토양중에서 선충이 생존하고 있다가 토양의 이동으로 선충이 전염된다. 5. 선충은 이삭과 뿌리에 많이 분포되어 있지만 성숙식물전체부분에 널리 분포되여 있다가 그것을 통해서 전염된다. 6. 5$0^{\circ}C$, 55$^{\circ}C$, 6$0^{\circ}C$의 온탕에서 5분간격으로 60분까지 종자처리를 하여 살선충효과와 발아와의 관계를 조사하였던 바 5$0^{\circ}C$에서는 60분간 처리하여도 효과가 없고 55$^{\circ}C$에서는 15~20분간, 6$0^{\circ}C$에서는 10~15분간만 처리하여도 효과가 있음을 알았다.

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소수성 마이크로 패턴을 갖는 Stripe 코팅용 슬롯 다이 헤드 구조 최적화 (Structure Optimization of a Slot-Die Head with a Hydrophobic Micro-Patterns for Stripe Coatings)

  • 유수호;이진영;박종운
    • 반도체디스플레이기술학회지
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    • 제18권2호
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    • pp.6-10
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    • 2019
  • In the presence of $\mu-tip$ for narrow stripe coating, there appears lateral capillary flow along the hydrophilic head lip because the $\mu-tip$ has some resistance to flow. It was known to be suppressed by increasing the contact angle of the head lip. In this paper, we have demonstrated by computational fluid dynamics(CFD) simulations that it can also be suppressed by the formation of micro-patterns on the shim and meniscus guide embedded into the slot-die head. To optimize the micro-patterned structure, we have performed simulations by varying the groove width, depth, and clearance. In the absence of micro-patterns, it is shown by experiment and simulation that the solution spreads to a distance of $1,300{\mu}m$ from the ${\mu}-tip$. In the presence of micro-patterns with the groove width and clearance of $50{\mu}m$, the distance the solution spreads is reduced to $260{\mu}m$. However, no further suppression in the capillary flow is observed with micro-patterns with the groove width of $40{\mu}m$ or less. It is also observed that the capillary flow is not affected by the groove depth if it is larger than $10{\mu}m$. We have shown that the distance the solution spreads can be reduced further to $204{\mu}m$ by coating a hydrophobic material (contact angle of $104^{\circ}$) on the surface of micro-patterns having the groove width and clearance of $50{\mu}m$.

Errors in light-emitting diodes positioning when curing bulk fill and incremental composites: impact on properties after aging

  • Abdulrahman A. Balhaddad;Isadora M. Garcia;Haifa Maktabi;Maria Salem Ibrahim;Qoot Alkhubaizi;Howard Strassler;Fabricio M. Collares;Mary Anne S. Melo
    • Restorative Dentistry and Endodontics
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    • 제46권4호
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    • pp.51.1-51.13
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    • 2021
  • Objectives: This study aimed to evaluate the effect of improper positioning single-peak and multi-peak lights on color change, microhardness of bottom and top, and surface topography of bulk fill and incremental composites after artificial aging for 1 year. Materials and Methods: Bulk fill and incremental composites were cured using multi-peak and single-peak light-emitting diode (LED) following 4 clinical conditions: (1) optimal condition (no angulation or tip displacement), (2) tip-displacement (2 mm), (3) slight tip angulation (α = 20°) and (4) moderate tip angulation (α = 35°). After 1-year of water aging, the specimens were analyzed for color changes (ΔE), Vickers hardness, surface topography (Ra, Rt, and Rv), and scanning electron microscopy. Results: For samples cured by single-peak LED, the improper positioning significantly increases the color change compared to the optimal position regardless of the type of composite (p < 0.001). For multi-peak LED, the type of resin composite and the curing condition displayed a significant effect on ΔE (p < 0.001). For both LEDs, the Vickers hardness and bottom/top ratio of Vickers hardness were affected by the type of composite and the curing condition (p < 0.01). Conclusions: The bulk fill composite presented greater resistance to wear, higher color stability, and better microhardness than the incremental composite when subjected to improper curing. The multi-peak LED improves curing under improper conditions compared to single-peak LED. Prevention of errors when curing composites requires the attention of all personnel involved in the patient's care once the clinical relevance of the appropriate polymerization reflects on reliable long-term outcomes.

하중 작용 방향(압축과 인발)과 말뚝의 직경이 말뚝 지지력에 미치는 영향 (The Influence of the Direction of Applied Load(Compression and Uplift) and the Diameter of the Pile on the Pile Bearing Capacity)

  • 이명환;윤성진
    • 한국지반공학회지:지반
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    • 제7권3호
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    • pp.51-64
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    • 1991
  • 말뚝기초 설계의 신뢰도및 경제성 향상을 위해서는 말뚝 지지력 예측의 신뢰도 제고가 필수적이다. 현재까지 수많은 말뚝지지력 예측 방법들이 제안되어 왔으나 말뚝재하시칩에 의하는 방법외에는 그 신뢰도가 보장되지 못하는 것이 현실이다. 그러나 말뚝재하시험은 상당한 시간과 비용이 소요되어 활성화 되지 못하였다. 말뚝의 주면마찰력을 선단부 재하의 반력으로 이용하는 SPLT의 개발은 이러한 문제점 들을 해소할 수 있는 방안으로 실무에 적용이 확산되고 있다. SPLT에서는 주면마찰력과 선단저항을 분리 측정하여 말뚝지지력 해석의 신뢰도를 높여줄 수 있는 이점이 있는 반 면, 주면마찰부에 작용하는 하중의 방향이 기존의 재하시험과 반대로 되는 문제점과 축소된 선단부 사용의 영향이 명확히 규명되지 못한 문제점이 해결되어야 할 과제로 제기되고 있다. 본 연구에서 는 이러한 영향을 알아보기 위하여 직경 406mm의 강관말뚝을 사용하여 SPLT의 1단계, 2단계 시험 후 재래식 재하시 칩을 수행하였다. 연구결과에 의하면 인발시의 주면마찰력은 압축시의 값보다 낮은 것으로 나타나 SPLT결과를 실 무에 적용하딴 안전율을 높여주는 효과를 주며, 축소된 선단부 사용은 치수효과 (scaleeffect)에 대한 적절한 해석후 적용할 수 있을 것으로 나타난다.

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