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Effects of Condiments upon ${\alpha}-Amylase$ activity (조미료(調味料)가 ${\alpha}-amylase$활성(活性)에 미치는 영향(影響))

  • Suh, Myung-Jah
    • Journal of Nutrition and Health
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    • v.9 no.1
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    • pp.104-109
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    • 1976
  • Condiments such as welsh onion, garlic, ginger, black pepper, red pepper, japanese pepper, mustard, horse radish, monosodium glutamate and sugar were ground by a homogenizer, and 0%, 1%, 5%, and 10% of each of the ground condiments were put into 0.2% ${\alpha}-amylase$ solution for storage at the temperature of $15^{\circ}C$. ${\alpha}-amylase$ activity then was measured by the Wohlgemuth method at 48-hour interval, and the following results were obtained. 1) Among the condiments, black pepper and sugar checked the ${\alpha}-amylase$ activity most, about 80% in comparison with control. 2) Welsh onion, garlic, mustard, and ginger checked the ${\alpha}-amylase$ activity, about 50% in comparison with control, irrespective of the time stored. 3) The low concentration of red pepper, horse radish, japanese pepper and mono-sodium glutamate checked the ${\alpha}-amylase$ activity about 50%, while the high one did below 75% in comparison with control. To conclude: all the condiments used in the experiment checked ${\alpha}-amylase$ activity.

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Exploration of optimum conditions for production of saccharogenic mixed grain beverages and assessment of anti-diabetic activity (잡곡당화음료 제조 최적 조건 탐색 및 항당뇨 활성 평가)

  • Lee, Jae Sung;Kang, Yun Hwan;Kim, Kyoung Kon;Yun, Yeong Kyeong;Lim, Jun Gu;Kim, Tae Woo;Kim, Dae Jung;Won, Sang Yeon;Bae, Moo Hoan;Choi, Han Seok;Choe, Myeon
    • Journal of Nutrition and Health
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    • v.47 no.1
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    • pp.12-22
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    • 2014
  • Purpose: This study was conducted to establish the production conditions through optimization of the production process of beverages using Aspergillus oryzae CF1001, and to analyze volatile compounds and antidiabetic activity. Methods: The optimum condition was selected using the response surface methodology (RSM), through a regression analysis with the following independent variables gelatinization temperature (GT, $X_1$), saccharogenic time (ST, $X_2$), and dependent variable; ${\Delta}E$ value (y). The condition with the lowest ${\Delta}E$ value occurred with combined 45 min ST and $50^{\circ}C$ GT. The volatile compounds were analyzed quantitatively by GC-MS. Results: Assessment of antidiabetic activity of saccharogenic mixed grain beverage (SMGB) was determined by measurement of ${\alpha}$-glucosidase inhibition activity, and glucose uptake activity and glucose metabolic protein expression by reverse transcriptase polymerase chain reaction (RT-PCR) and western blot analysis. Results of volatile compounds analysis, 62 kinds of volatile compounds were detected in SMGB. Palmitic acid (9.534% ratio), benzaldehyde (8.948% ratio), benzyl ethyl ether (8.792% ratio), ethyl alcohol (8.35% ratio), and 2-amyl furan (4.826% ratio) were abundant in SMGB. We confirmed that ${\alpha}$-glucosidase inhibition activity, glucose uptake activity, and glucose-metabolic proteins were upregulated by SMGB treatment with concentration dependent manner. Conclusion: Saccharogenic mixed grain beverage (SMGB) showed potential antidiabetic activity. Further studies will be needed in order to improve the taste and functionality of SMGB.

Eco-friendly Control of Whiteflies by Two-Fluid Fogging System with Natural Substances in Greenhouses (이류체 포그시스템 및 천연물을 이용한 친환경적 가루이 방제)

  • Kim, Sung-Eun;Lee, Sang-Don;Lee, Moon-Haeng;Kim, Young-Shik
    • Journal of Bio-Environment Control
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    • v.21 no.2
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    • pp.114-119
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    • 2012
  • We have conducted 4 experiments to develop the most environmental and effective use of the two-fluid fog system to prevent and exterminate whiteflies in tomato cultivation. In particular, these experiments used Vitamini tomatoes grown in stand-alone greenhouses at Buyeo Tomato Experiment Station as subjects. Each experiment utilized the fog system in a different way. The first experiment provided the control group, which was subject to the two-fluid fog system without additional humidity control. In the second experiment, the two-fluid fog system controlled the humidity level to be above 70%. The third and the fourth experiment utilized natural substances, which were 1.5 mg/L of Neem Oil and 2 mg/L of Oleic acid respectively, without additional humidity control. From the first experiment, we could observe that a simple use of the two-fluid fog system decreased the density of whiteflies in the greenhouses. This impact of the fog system on whiteflies was greater in the second experiment. By comparing the first and the second experiment, we concluded that whiteflies are more effectively prevented by maintaining a higher humidity level via the fog system's smaller water droplets that float in the air for longer time than the standard fog system in rather dry condition. In the third and the fourth experiments, the extermination level was 78% and 76.4% respectively, comparing only 53% in the first experiment without the humidity control. Therefore, using the natural substances in addition to the humidity control increases the extermination effectiveness. Considering the similar results from the 3rd and the 4th experiments, Oleic acid has a greater appeal for its lower price. Using the two-fluid fog system to both control the humidity on a daily basis and spray the substances for occasional extermination would reduce labor cost and increase production in an environmental way.

Outlook on Variation of Water Resources in Korea under SRES A2 Scenario (A2 시나리오에 따른 국내 수자원의 변동성 전망)

  • Bae, Deg-Hyo;Jung, Il-Won;Lee, Byong-Ju
    • Journal of Korea Water Resources Association
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    • v.40 no.12
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    • pp.921-930
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    • 2007
  • The objective of this study is to present temporal-spatial variation of water resources on climate change impacts using the IPCC SRES A2 scenario and dynamical downscaling of the results (using the MM5 model with a resolution of 27km by 27km) at 139 sub-basins in Korea. The variation of runoff shows differences in the change of rate according to the each sub-basins and analysis durations. It has increased in the sub-basins located in Han river basin and east part of it, the other basins have decreased. In seasonal analysis, runoff in autumn and winter have increased, while in spring and summer have decreased. The results of frequency analyzing classified runoff(Low flow(Q$\leq$5mm), Normal flow(5$\geq$100mm)) show that low flow increase in most of the sub-basins for 2031-2060 and 2061-2090. In the case of high flow, it have higher frequency ranging from -100% to 500% than low flow. Regardless of the variation of mean runoff, maximum discharge appeared to be increase in process of time. The regression method is used to figure out the relationship between the rate of runoff change and mean temperature, mean precipitation under A2 scenario. The mean actual evapotranspirations from the regression equations increased by 3.4$\sim$5.3% for the change of $1^{\circ}C$. Also, for the precipitation change of $\pm$10%, runoff variety range is -18.2$\sim$+12.4% in Han River, -21.6$\sim$+14.6% in Nakdong River, -17.5$\sim$+11.5% in Gum River, -18.4$\sim$+10.6% in Sumjin River, -19.9$\sim$+12.7% Youngsan River basin.

Performance Evaluation of Bio-Membrane Hybrid Process for Treatment of Food Waste Leachate (음식물 침출수 청정화를 위한 파일롯 규모의 생물-분리막 복합공정의 성능 평가 연구)

  • Lee, Myung-Gu;Park, Chul-Hwan;Lee, Do-Hoon;Kim, Tak-Hyun;Lee, Byung-Hwan;Lee, Jin-Won;Kim, Sang-Yong
    • KSBB Journal
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    • v.23 no.1
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    • pp.90-95
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    • 2008
  • In this study, a combined process of sequential anaerobic-aerobic digestion (SAAD), fluidized-bed bioreactor (FBBR), and ultrafiltration (UF) for the treatment of small scale food waste leachate was developed and evaluated. The SAAD process was tested for performance and stability by subjecting leachate from food waste to a two-phase anaerobic digestion. The main process used FBBR composed of aerators for oxygen supply and fluidization, three 5 ton reaction chambers containing an aerobic mesophilic microorganism immobilized in PE (polyethylene), and a sedimentation chamber. The HRTs (hydraulic retention time) of the combined SAAD-FBBR-UF process were 30, 7, and 1 day, and the operation temperature was set to the optimal one for microbial growth. The pilot process maintained its performance even when the CODcr of input leachate fluctuated largely. During the operation, average CODcr, TKN, TP, and salt of the effluent were 1,207mg/L, 100mg/L, 50 mg/L, and 0.01 %, which corresponded to the removal efficiencies of 99.4%, 98.6%, 89.6%, and 98.5%, respectively. These results show that the developed process is able to manage high concentration leachate from food waste and remove CODcr, TKN, TP, and salt effectively.

An Initiative Study on Relationship between Algal Blooms and Asian Dust for Regulation of Algal Blooms (조류 성장 억제를 위한 녹조 및 적조 발생과 황사의 상관관계 초기적 연구)

  • Kim, Tai-Jin;Jeong, Jaechil;Seo, Rabeol;Kim, Hyung Moh;Kim, Dae Geun;Chun, Youngsin;Park, Soon-Ung;Yi, Sehyoon;Park, Jun Jo;Lee, Jin Ha;Lee, Jay J.;Lee, Eun Ju
    • KSBB Journal
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    • v.29 no.4
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    • pp.285-296
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    • 2014
  • Although the problems of the algal blooms have been world-widely observed in freshwater, estuary, and marine throughout the year, it is not yet certain what are the basic causes of such blooms. Consequently, it is very difficult to predict when and where algal blooms occur. The constituents of the Asian dust are in a good agreement with the elements required for the algal growth, which suggests some possible relationship between the algal blooms and the Asian dust. There have been frequently algal blooms in drinking water from rivers or lakes. However, there is no any algal blooms in upwelling waters where the Asian dust cannot penetrate into the soil due to its relatively weak settling velocity (size of particles, $4.5{\pm}1.5{\mu}m$), which implies the possible close relationship of the Asian dust with algal blooms. The present initiative study is thus intended firstly in Korea to illustrate such a relationship by reviewing typical previous studies along with 12 years of weekly iron profiles (2001~2012) and two slant culture experiments with the dissolved Asian dust. The result showed bacterial suspected colonies in the slant culture experiment that are qualitatively in a good agreement with the recent Japanese studies. Since the diatoms require cheap energy (8%) compared to other phytoplankton (100%) to synthesize their cell walls by silicate, the present results can be used to predict algal blooms by diatoms if the concentrations of iron and silicate are available during spring and fall. It can be postulated that the algal blooms occur only if the environmental factors such as light, nutrients, calm water surface layer, temperature, and pH are simultaneously satisfied with the requirements of the micronutrients of mineral ions supplied by the Asian dust as enzymatic cofactors for the rapid bio-synthesis of the macromolecules during algal blooms. Simple eco-friendly methods to regulate the algal blooms are suggested for the initial stage of blooming with limited area: 1) to cover up the water surface with black curtain and inhibit photosynthesis during the day time, 2) to blow air (20.9%) or pure oxygen into the bottom of the water and inhibit rubisco for carbon uptake and nitrate reductase for nitrogen uptake activities in algal growth during the night, 3) to eliminate the resting spores or cysts by suction of bottom sediments as deep as 5 cm to prevent the next year germinations.

Strategy for Bio-Diversity and Genetic Conservation of Forest Resources in Korea (생물종(生物種) 다양성(多樣性) 및 삼림유전자원(森林遺傳資源) 보존(保存) 전략(戰略))

  • Park, Young Goo
    • Journal of Korean Society of Forest Science
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    • v.83 no.2
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    • pp.191-204
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    • 1994
  • Due to its topographic complexities and various climatical condition, Korea exhibits diverse forest types. Dominant tree species in this zone are Quercus spp., Betula spp., Zelkova spp., Fraxinus spp., Pinus densiflora, Pinus koraiensis, and Pinus thunbergii ete. Genetic conservation in forest species in Korea there are three ways ; one is in situ, other is ex situ and third is in-facility conservation. In situ conservation include that are the present status of conservation of rare and endangered flora and ecosystem, the reserved forest, the national and provincial park, and the gene pool of natural forests. Ex situ conservation means to be established the new forest from in situ forest stands, progeny and provenance test populations, seed orchard and clone banks, and gene conservation in-facility. As a tool for low temperature storage, several aspects on in vitro system were studied ; (1) establishment of in vitro cultures from juvenile and/or rejuvenated tissues, (2) induction of multiple shoots from the individual micropropagules, (3) elongation of the proliferated shoots. Studies on cold storage for short-and long-term maintenance of in vitro cultures under $4^{\circ}C$ in the refrigerator were conducted. For the cryopreservation at $-196^{\circ}C$, various factors affecting survivability of the plant materials are being examined. The necessity of gene conservation of forest trees is enlarged not only to increase the adaptability for various environments but also to gain the breeding materials in the future. For effective gene conservation of forest trees, I would like to suggest followings ; 1. Forest stands reserved for other than the gene conservation purposes such as national parks should be investigated by botanical and gene-ecological studies for selecting bio-diversity and gene conservation stands. 2. Reserved forest for gene pool should be extented both economically important tree spp. and non-economical species. 3. Reserved forest for progeny test and clone bank should be systematically investigated for the use of Ex situ forest gene conservation. 4. We have to find out a new methodology of genetic analysis determining the proper and effective size of subpopulation for in situ gene conservation. 5. We should develop a new tree breeding systems for successful gene conservation and utilization of the genetic resources. 6. New method of in-facility gene conservation using advanced genetic engineering should be developed to save time and economic resources. 7. For the conservation of species with short-life span of seed or shortage of knowledge of seed physiology, tissue culture techniques will be played a great role for gene conservation of those species. 8. It is are very useful conservation not only of genes but of genotypes which were selected already by breeding program. 9. Institutional and administrative arrangements including legistlation must be necessarily taken for gene conservation of forest trees. 10. It is national problems for conservation of forest resources which have been rapidly destroyed because of degenerating environmental condition and of inexperienced management system of bio-diversity and gene conservation. 11. In order to international cooperation for exchanging data of bio-diversity and gene conservation, we should connect to international net works as soon as possible.

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Quality Improvement of High Volume Fly Ash Concrete due to Early Strength Gain Admixture (조강형 혼화제에 의한 플라이애시 다량 치환 콘크리트의 품질 향상)

  • Han, Cheon-Goo;Park, Jong-Ho;Lee, Joung-Ah
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.4 no.4
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    • pp.117-124
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    • 2009
  • The purpose of the study was to improve quality of high volume fly ash concrete. The study evaluated on the possibility of early quality improvement of high volume fly ash concrete with early strength gain admixture ('GA' below) developed by the preceding research. The study regarded applying naphthalene admixture ('NA' below) to mix proportion substituting FA 15 % to be plain. In the event of substituting FA 20, 25 and 30 %, the study compared engineering properties of concrete with plain by applying GA. Because of features of fresh concrete, fluidity falls down when GA is applied. Therefore, its use amount shall be increased. Only, in W/B 60 %, it was beneficial since slump loss was reduced about 35~70 mm than plain. The study could see that AE use should be increased proportionally since air content was reduced by coming from AE absorption operation of unburned coal content included in FA according to an increase in the amount of FA use. Reduction effect of bleeding could be anticipated since the amount of bleeding appeared at least in FA 20 %. Because of hardened concrete, time of setting appeared in the same level as plain when GA was applied. Therefore, it is judged that delay of setting can be reduced. In compressive strength, the study could check the same strength development as plain when GA was applied, having nothing to do with W/B and curing temperature. However, it is thought that we shall pay attention to GA use in the event of FA 30 % substitution. Freezing and melting resistance had less early value than plain. However, it is judged that there will be no problem of frost resistance since there is no a large difference between freezing and melting resistance and plain in overall. In accelerated neutralization, it was analyzed that a problem of weakening in neutralization appointed as a demerit when FA was applied in mass in proportion with GA use could be settled to some extent.

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Studies on Quality Changes and Antioxidant Activity During the Fermentation of the Salt Fermented Whangseoke (황석어(Collichthys nireatus Jordan et starks) 젓갈의 숙성과정 중 품질변화와 항산화작용에 관한 연구)

  • Kim, Ji-Sang;Moon, Gap-Soon;Lee, Kyung-Hee;Lee, Young-Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.2
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    • pp.171-176
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    • 2006
  • The effect of storage temperature on the quality and antioxidative activity Whangseoke sauce was studied over a period of 240 days. Fermented Whangseoke with $25\%$ salt were stored at $25^{\circ}C$. The quality change and antioxidant activity of Whangseoke in linoleic acid emulsion was evaluated with various parameters including acids values, peroxide values, TBA values, reducing sugar, brown color intensity, electron donating ability and reducing power at various time intervals for 240 days of storage. In general, it was observed, in all sample, that peroxide values, brown color intensity, electron donating ability and reducing power gradually increased, while reducing sugar decreased during storage at $25^{\circ}C$. The antioxidative activities of fermented Whangseoke were determined on tile linoleic acid emulsion system. The results showed that Whangseoke had antioxidant activity. These results suggest that antioxidant activity of Whangseoke seemed to influence by Maillard reaction products during the storage periods.

Assessment of Optimum Hydraulic Retention Time (HRT) for Maximum Biogas Production and Total Volatile Solid (TVS) Removal Efficiency of Semi-Continuously Fed and Mixed Reactor (SCFMR) Fed with Dairy Cow Manure (젖소분뇨로부터 최대 바이오가스 생산과 유기물 제거효율을 달성하기 위한 반건식 간헐주입 연속혼합 혐기성반응조의 최적 수리학적 체류시간 도출을 위한 연구)

  • Kang, Ho;Kim, Sun-Woo;Jeong, Ji-Hyun;Ahn, Hee-Kwon;Jung, Kwang-Hwa
    • Journal of Korean Society of Environmental Engineers
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    • v.37 no.12
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    • pp.696-704
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    • 2015
  • This study was carried out to evaluate the optimum operational condition of Semi-continuously Fed and Mixed Reactor (SCFMR) to treat the dairy cow manure and saw dust mixture. Step-wise increase in organic loading rates (OLRs) or decrease in hydraulic retention times (HRTs) were utilized until the biogas volume became significantly decreased at mesophilic temperature ($35^{\circ}C$). The optimum operating condition of the SCFMR fed with TS 13% dairy cow manure and saw dust mixture was found to be an HRTs of 25 days and its corresponding OLRs of $4.45kg\;VS/m^3-day$. At this condition the biogas and methane production rates were 1.44 v/v-d and 1.12 v/v-d (volume of biogas per volume of reactor per day), respectively and the TVS removal efficiency of 37% was achieved. The successful operation with such a high OLR was due to the high reactor alkalinity concentration of 14,500~15,600 mg/L as $CaCO_3$ as a result of the characteristic of the original substrate, dairy cow manure and saw dust mixture whose alkalinity was more than 8,000 mg/L as $CaCO_3$. The parameters for the reactor stability, the ratios of volatile acids and alkalinity concentrations (V/A) and the ratio of propionic acid and acetic acid concentrations (P/A) appeared to be 0.11 and 0.43, respectively, that were greatly stable in operation. Free ammonia toxicity was not experienced due to the long term acclimation by the reactor TS content ranged 7.2~10.4% during the entire operational period.