• Journal of Internet Computing and Services
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• v.2 no.1
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• pp.31-38
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• 2001

Speech database built of phonemes is significant in the studies of speech recognition, speech synthesis and analysis, Phoneme, consist of voiced sounds and unvoiced ones, Though there are many feature differences in voiced and unvoiced sounds, the traditional algorithms for detecting the boundary between phonemes do not reflect on them and determine the boundary between phonemes by comparing parameters of current frame with those of previous frame in time domain, In this paper, we propose the assort algorithm, which is based on a block and reflecting upon the feature differences between voiced and unvoiced sounds for phoneme segmentation, The assort algorithm uses the distance measure based upon MFCC(Mel-Frequency Cepstrum Coefficient) as a comparing spectrum measure, and uses the energy, zero crossing rate, spectral energy ratio, the formant frequency to separate voiced sounds from unvoiced sounds, N, the result of out experiment, the proposed system showed about 79 percents precision subject to the 3 or 4 syllables isolated words, and improved about 8 percents in the precision over the existing phonemes segmentation system.

• Journal of the Korean Geotechnical Society
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• v.21 no.6
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• pp.147-154
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• 2005

This study deals with the calibration of low-frequency water content reflectometer for measuring the volumetric water content of soils in landfill final cover layer, and the validity of calibrations was evaluated by electrical conductivity and index properties of the soils. Linear calibrations concerning volumetric water content to WCR period provided good agreement with the data. Analysis of the calibration data indicates that the slope of the calibration decreases as the electrical conductivity of the soil increases. Lower slopes correspond to soils with greater clay content, organic content, liquid limit, and plasticity index, which typically have higher electrical conductivity. It could be well explained that WCR can operate in a lower frequency range.

• Journal of the Korean Institute of Gas
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• v.17 no.2
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• pp.85-89
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• 2013

The purpose of the development of KC-1 LNG cargo containment system is reduction in royalty and increase in competitiveness of shipbuilding industry. An assessment of structure safety for LNG cargo containment system under sloshing load due to ship motion has become an important design element. The ideal way is to implement fully interaction of the fluid domain and the cargo containment system. However the irregular sloshing pressure were idealized in the form of a triangular wave for safety assessment because the fluid- structure interaction analysis is taken the extensive computation time and difficult to ensure the accuracy of the results. In this study, the sloshing load was assumed to be a triangular wave with a maximum pressure of 10 bar during 15/1000 seconds. In the analytic results, the basic insulation panel of KC-1 LNG cargo containment system was assessed to be structurally safe for sloshing load.

• Journal of Broadcast Engineering
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• v.25 no.4
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• pp.569-575
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• 2020

The serial arc is one of factors causing electrical fires. Over past decades, various researches have been carried out to detect arc occurrences. Even though frequency analysis, wavelet, and statistical features have been used, additional steps such as transformation and feature extraction are required. On the contrary, deep learning models directly use the raw data without any feature extraction processes. Therefore, the usage of time-domain data is preferred, but the performance is not satisfactory. To solve this problem, subsequent 1-D signals are transformed into 2-D data that can feed into a convolutional neural network (CNN). Experiments validated that CNN model outperforms deep neural network (DNN) by the classification accuracy of 8.6%. In addition, data augmentation is utilized, resulting in the accuracy improvement by 14%.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.4
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• pp.1343-1348
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• 2015

Human UHRF1 (ubiquitin-like PHD and RING finger domain-containing 1) has been reported to be over-expressed in many cancers, but its role in ovarian cancer remains elusive. Here, we determined whether knockdown of UHRF1 by lentivirus-mediated shRNA could inhibit ovarian cancer cell growth. Lentivirus-mediated short hairpin RNAs (lv-shRNAs-UHRF1) were designed to trigger the gene silencing RNA interference (RNAi) pathway. The efficiency of lentivirus-mediated shRNA infection into HO-8910 and HO-8910 PM cells was determined using fluorescence microscopy to observe lentivirus-mediated GFP expression and was confirmed to be over 80 percent. UHRF1 expression in infected HO-8910 and HO-8910 PM was evaluated by real-time PCR and Western blot analysis. The Cell Counting Kit-8 (CCK-8) assay was used to measure cell viability; flow cytometry and Hoechst 33342 assay was applied to measure cell cycle arrest and apoptosis. Cell invasion was assessed using transwell chambers. Our results demonstrated that the loss of UHRF1 promoted HO-8910 and HO-8910 PM cell apoptosis, while inhibiting cell proliferation. In addition, UHRF1 knockdown significantly inhibited the invasion of human ovarian cancer cells. In the present study, we also showed that depleting HO-8910 cells of UHRF1 caused activation of the DNA damage response pathway, with the cell cycle arrested in G2/M-phase. The DNA damage response in cells depleted of UHRF1 was illustrated by phosphorylation of CHK (checkpoint kinase) 2 on Thr68, phosphorylation of CDC25 (cell division control 25) on Ser 216 and phosphorylation of CDK1 (cyclin-dependent kinase 1) on Tyr 15.

• BMB Reports
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• v.31 no.2
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• pp.111-116
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• 1998

HLA-DR4 is the dominant allele of MHC class II genes in Koreans. In particular, the $DRB1^*0405$ subtype has been reported to be almost exclusively expressed in Far East Asians, and has also been observed to be strongly associated with rheumatoid arthritis in Koreans and the Japanese. Identification of this specific allele has been mainly performed by PCR-based methods, which is often time consuming, costly, and involves tedious procedures such as the isolation of genomic DNA, PCR, and gel electrophoresis. To develop a more convenient tool for screening vast amounts of samples as well as to generate reagents which might also be used in other applications, in this study, antibodies were produced against this specific HLA subtype. By PCR, an allelespecific region covering the ${\beta}1$ domain of $DRB1^*0405$ was amplified and recombinantly expressed in E.coli. Immunization of Lewis rats with the purified protein yielded an allele specific antiserum. Western blot analysis showed the selective detection of the HLA-DR ${\beta}-chain$. Using this antiserum, established cell lines and peripheral blood lymphocytes were analyzed on their HLA haplotype by fluorescence activated flow cytometry. These novel antibodies will provide a powerful tool in the detection and investigation of DR4 alleles.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.6A
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• pp.676-684
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• 2008

In digital video broadcasting-terrestrial (DVB-T), which is the European digital terrestrial television standard, the orthogonal frequency division multiplexing (OFDM) has been adopted for signal transmission. The main reasons using OFDM are to increase the robustness against the frequency selective fading and impulse noise, and to use available bandwidth efficiently. However, channel variation within an OFDM symbol destroys orthogonality between subcarriers, resulting in inter-carrier interference (ICI), which increases an error floor in proportional to maximum Doppler spread. This paper provides an ICI analysis in both time and frequency domains while existing literatures analyze the ICI effects mainly in frequency domain and proposes the algorithms that estimate the channel impulse response and channel variation using least square (LS) algorithm which is the most simple channel estimation technique. And we propose adaptive channel estimation algorithm that estimates the velocity of terminals. The simulation results show that proposed algorithm has similar performance with about 1.5% computational complexity of noise and ICI reduction LS algorithm in low speed environments.

• The Journal of the Korea Contents Association
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• v.6 no.1
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• pp.1-7
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• 2006

This paper proposes a new method that reduces the complexity of the combined system, which consists of the selective mapping(SLM) and the partial transmit sequence(PTS) in the Orthogonal Frequency Division Multiplexing(OFDM). In order to reduce the Peak-to-Average Power Ratio(PAPR), many techniques have been developed such as SLM and PTS. and their combined systems. However the method that simply combines PTS and SLM to improve the effectiveness of a system increases the complexity of calculation. The scheme employed in this research suggests a system that combines PTS and SLM reducing the complexity via the N($N=2^n$)-point inverse fast Fourier transforms(IFFT), which uses the decimation in time domain not only to improve PAPR but also to reduce the amount of calculation. The proposed method reduces the complexity by multiplying a phase sequence and a subblock index to the data sequences in the middle of IFFT, which yields an optimal sequence with minimal PAPR. The superiority of the proposed method is demonstrated by simulation results and analysis.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.6
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• pp.483-489
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• 2017

An ultrasonic technique was developed to characterize the resonance behavior of a microcantilever operating in a megahertz regime. A high-power ultrasonic pulser and a contact transducer were employed to excite the silicon microcantilever, and a Michelson interferometer was used to obtain the time domain waveform. The natural frequency of the microcantilever was evaluated through the fast Fourier transform of the signal, and a numerical analysis using the finite element method confirmed the measurement data. The present study proposes a novel and facile method to evaluate nanoscale materials and structures with high sensitivity compared to conventional approaches.

• Journal of Animal Science and Technology
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• v.54 no.3
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• pp.157-163
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• 2012

The male reproduction is precisely controlled by a number of intrinsic and extrinsic factors. These factors usually involve in expressional regulation of various molecules influencing on sperm production in the testis. A number of ways are employed to control the transcription of specific genes, including epigenetic modifications of DNA and histone molecules. DNA methylation of CpG dinucleotides is a commonly used regulatory mechanism for testicular genes associated with the fertility. Previous studies have demonstrated the infertility induced by improper DNA methylation of these genes. In the present research, we attempted to determine transcriptional expression of some of these genes in the rat testis at different postnatal ages using real-time PCR analysis. These genes include neurotrophin 3 (Ntf3), insulin-like growth factor II (Igf2), JmjC-domain-containing histone demethylase 2A 1 (Jhm2da), paired box 8 transcription factor (Pax8), small nuclear ribonucleoprotein polypeptide N (Snrpn), and 5,10-methylenetetrahydrofolate reductase (Mthfr). The expression levels of Ntf3, Igf2, and Snrpn genes were the highest at the neonatal age, followed by transient decreases at the prepubertal age. Expression of Jhm2da and Mthfr genes were continuously increased from the neonate to 1 year of age. The levels of Pax8 mRNA at the early ages were higher than those at the later ages of postnatal development. These findings suggest that expression of some fertility-associated testicular genes in the rat during postnatal period could be differentially regulated by the control of the degree of DNA methylation.