• Proceedings of the Korean Society of Toxicology Conference
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• 2006.11a
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• pp.65-74
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• 2006

Modem drug discovery requires rapid pharmacokinetic evaluation of chemically diverse compounds for early candidate selection. This demands the development of analytical methods that offer high-throughput of samples. Naturally, liquid chromatography / tandem mass spectrometry (LC-MS/MS) is choice of the analytical method because of its superior sensitivity and selectivity. As a result of the short analysis time(typically 3-5min) by LC-MS/MS, sample preparation has become the rate- determining step in the whole analytical cycle. Consequently tremendous efforts are being made to speed up and automate this step. In a typical automated 96-well SPE(solid-phase extraction) procedure, plasma samples are transferred to the 96-well SPE plate, internal standard and aqueous buffer solutions are added and then vacuum is applied using the robotic liquid handling system. It takes only 20-90 min to process 96 samples by automated SPE and the analyst is physically occupied for only approximately 10 min. Recently, the ultra-high flow rate liquid chromatography (turbulent-flow chromatography)has sparked a huge interest for rapid and direct quantitation of drugs in plasma. There is no sample preparation except for sample aliquotting, internal standard addition and centrifugation. This type of analysis is achieved by using a small diameter column with a large particle size(30-5O ${\mu}$m) and a high flow rate, typically between 3-5 ml/min. Silica-based monolithic HPLC columns contain a novel chromatographic support in which the traditional particulate packing has been replaced with a single, continuous network (monolith) of pcrous silica. The main advantage of such a network is decreased backpressure due to macropores (2 ${\mu}$m) throughout the network. This allows high flow rates, and hence fast analyses that are unattainable with traditional particulate columns. The reduction of particle diameter in HPLC results in increased column efficiency. use of small particles (<2 urn), however, requires p.essu.es beyond the traditional 6,000 psi of conventional pumping devices. Instrumental development in recent years has resulted in pumping devices capable of handling the requirements of columns packed with small particles. The staggered parallel HPLC system consists of four fully independent binary HPLC pumps, a modified auto sampler, and a series of switching and selector valves all controlled by a single computer program. The system improves sample throughput without sacrificing chromatographic separation or data quality. Sample throughput can be increased nearly four-fold without requiring significant changes in current analytical procedures. The process of Bioanalytical Method Validation is required by the FDA to assess and verify the performance of a chronlatographic method prior to its application in sample analysis. The validation should address the selectivity, linearity, accuracy, precision and stability of the method. This presentation will provide all overview of the work required to accomplish a full validation and show how a chromatographic method is suitable for toxirokinetic sample analysis. A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method developed to quantitate drug levels in dog plasma will be used as an example of tile process.

• Journal of Korea Water Resources Association
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• v.47 no.3
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• pp.297-306
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• 2014

In this study, EFDC (Environmental Fluid Dynamics Code) model was used to simulate the salinity distribution for sea water inflow and rainfall runoff. The flowrate was given to the boundary conditions, which can be calculated by areal-specific flowrate method from the measured flowrate of the representative outfall. The boundary condition of the water elevation can be obtained from the hourly tidal elevation. The flowrate from the outfall can be calculated using the condition of the 245 mm raifall. The simulation results showed that at Sites 1~2 and the Mangsan island (Site 4) the salinity becomes 0 ppt after the rainfall. However, the salinity is 30 ppt when there is no rainfall. Time series of the salinity changes were compared with the measured data from January 1 to December 31, 2010 at the four sites (Site 2~5) of Yongwon channel. Lower salinities are shown at the inner sites of Yongwon channel (Site 1~4) and the sites of Songjeong river (Site 7~8). The intensive investigation near the Mangsan island showed that the changes of salinity were 21.9~28.8 ppt after the rainfall of 17 mm and those of the salinity were 2.33~8.05 ppt after the cumulative rainfall of 160.5 mm. This means that the sea water circulation is blocked in Yongwon channel, and the salinity becomes lower rapidly after the heavy rain.

• Korean Journal of Agricultural and Forest Meteorology
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• v.9 no.4
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• pp.217-227
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• 2007

The western cherry fruit fly, Rhagoletis indifferens Curran (Diptera:Tephritidae), is the most important pest of cultivated cherries in the Pacific Northwest area of the United States, being widely distributed throughout Oregon, Washington, Montana, Utah, Idaho, Colorado and parts of Nevada. The control of R. indifferens has been based on calendar sprays after its first emergence because of their zero tolerance for quarantine. Therefore, a good prediction model is needed for the spray timing. This study was conducted to obtain the empirical population dynamic information of R. indifferens after overwintering in the major cherry growing area of the Pacific Northwest of the United States, where the information is critically needed to develop and validate the prediction model of the fruit fly. Adult fly populations were monitored by using yellow sticky and emergence traps. Larvae growth and density in fruits were observed by fruit sampling and the pupal growth and density were monitored by pupal collection traps. The first adult was emerged around mid May and a large number of adults were caught in early June. A fruit had more than one larva from mid June to early July. A large number of pupae were caught in early July. The pupae were collected in various period of time to determine the effect of pupation timing and the soil moisture content during the winter. A series of population density data collected in each of the developmental stage were analyzed and organized to provide more reliable validation information for the population dynamic models.

• Journal of Korea Port Economic Association
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• v.26 no.1
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• pp.222-233
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• 2010

This paper aims at predicting the BDI from Jan. to Dec. 2010 using such econometric techniues of the univariate time series as stochastic ARIMA-type models and Hodrick-Prescott filtering technique. The multivariate cause-effect econometric model is not employed for not assuring a higher degree of forecasting accuracy than the univariate variable model. Such a cause-effect econometric model also fails in adjusting itself for the post-sample. This article introduces the two ARIMA models and five Intervention-ARIMA models. The monthly data cover the period January 2000 through December 2009. The out-of-sample forecasting performance is compared between the ARIMA-type models and the random walk model. Forecasting performance is measured by three summary statistics: root mean squared error (RMSE), mean absolute error (MAE) and mean error (ME). The RMSE and MAE indicate that the ARIMA-type models outperform the random walk model And the mean errors for all models are small in magnitude relative to the MAE's, indicating that all models don't have a tendency of overpredicting or underpredicting systematically in forecasting. The pessimistic ex-ante forecasts are expected to be 2,820 at the end of 2010 compared with the optimistic forecasts of 4,230.

• Journal of Korean Society for Geospatial Information Science
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• v.23 no.2
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• pp.3-9
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• 2015

Since changes in land use in urban space cause traffic volume and it is closely related to traffic accidents. Therefore, an analysis on the causes of traffic accidents is judged to be an essential factor to establish the measure to reduce traffic accidents. In this regard, the analysis was conducted on the clustering by using the nearest neighbor indexes with regard to the occurrence frequencies of commercial and residential zone based on traffic accident data of the past five years (2009-2013) with the target of local small-medium sized city, Jinju-si. The analysis results, obtained in this study, are as follows: the occurrence frequency of traffic accidents was the highest in spring and the lowest in winter respectively. The clustering of traffic accident occurrence at nighttime was stronger than at daytime. In addition, terms of the analysis on the clustering of traffic accident according to land use, changes according to the seasons was not significant in commercial areas, while clustering density in winter tended to become significantly lower in residential areas. The analysis results of traffic accident types showed that the side-right angle collision of cars was the highest in frequency occurrence, and widespread in both commercial areas and residential areas. These results can provide us with important information to identify the occurrence pattern of traffic accidents in the structure of urban space, and it is expected that they will be appropriately utilized to establish measures to reduce traffic accidents.

• The Journal of the Korea Contents Association
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• v.12 no.9
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• pp.357-368
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• 2012

The purpose of this study is to empirically examine the impact of enterprise R&D investment on international trade in Korea under the new Normal Era. In order to test whether the time series data of trade variables are stationary or not, we put in operation unit root test and cointegration test. Based on VECM (Vector Error Correction Model), we also apply impulse response functions and variance decomposition to estimate the dynamic effects in the short-run and long-run. The results show that the relationship between enterprise R&D investment and international trade (export and import) exists in the long-run as well as in the short-run. The results of applying impulse response functions and variance decomposition also indicate that the impact of enterprise R&D investment on international trade is positive, and a significant portion of fluctuations in the trade variable is explained by enterprise R&D investment. Therefore, enterprise R&D investment must be continuously increased to improve economic growth with promoting trading competition power in Korea under the new Normal Era.

• Journal of the Korean Society for Marine Environment & Energy
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• v.9 no.1
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• pp.55-62
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• 2006

Correlations between air-temperature variation and SST variation around Jeju Island have been studied with data JRMO($1924{\sim}2004$) and NFRDI($l971{\sim}2000$). Air-temperature has increased about $0.02^{circ}C/year$ for the period of $1924{\sim}2004$ but relatively high 0.035/year for the last 30 years. SST has increased about $0.024^{circ}C/year$ for the period of $1971{\sim}2000$ and relatively high $0.047^{circ}C/year$ in December. According to the analysis of time series of the two kind of variation, the SST and air-temperature are positively correlated. They are generally in phase, and SST anomaly is similar to air-temperature anomaly as well. Consequently, SST variation has high correlation with air-temperature variation around Jeju Island.

• Korea journal of population studies
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• v.34 no.1
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• pp.159-178
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• 2011

Korea has been widely recognized as the most successful country for reversal of the rise in sex ratio at birth (from the mid-1980s to the mid-1990s) in a short period of time. However, unusually high sex ratios at birth are still observed in most regions as parity increases. Given that imbalanced sex ratios at high birth orders are mostly due to son-selective abortion, it still remains questionable whether son-selective reproductive behavior has vanished in Korea. The main purpose of this study is to analyze the pattern of changing trends and socioeconomic differentials in sex ratio at birth. Micro-data from birth registration for 2009 are utilized. Attention is focused on analyzing sex ratios at birth in Youngnam region according to age of mother, parity, educational attainment of parents, and occupation of parents. A series of simulations are also conducted in this paper to show how prenatal sex screening and son-selective abortion have affected the level of sex ratio at birth for years 1994, 2005 and 2009.

• Journal of the Korean Chemical Society
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• v.38 no.9
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• pp.640-652
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• 1994

This work is undertaken to determine alcohol homologues so-called fusel oil that may be present in the Korean folk sojues (distilled liquor) made from grains and to describe sample preparation and analytical method by GC-FID-MS. Solid phase extraction method for sample preparation by using porous styrene divinyl benzene polymer (Porapak Q) was compared with steam distillation and solvent extraction method. Retention behaviors of homologous series of alcohols were also studied. Log values of retention time, molecular weight, boiling point, and capacity factor of alcohols showed linear correlations to the carbon number of an alcohol, to the oven temperature, and to the dielectric constant. Components such as methyl alcohol, n-propyl alcohol, isobutyl alcohol, isopentyl alcohol, and phenethyl alcohol have been identified. The more amount of isopentyl alcohol than other alcohols are contained in the Korean folk sojues, while that of n-propyl alcohol are contained in Chinese kaoliangchiew. Degree of similarity or dissimilarity and classification of the individual samples were discussed using multivariate statistical analysis(principal components analysis) based on GC data.

• Geophysics and Geophysical Exploration
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• v.2 no.4
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• pp.180-183
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• 1999

A proto-type seismic measurement system with a reference was developed to improve S/N (signal-to-noise ratio) of seismic data, especially in noisy urban areas. Two pairs of correlation measurements (the one for microphone and geophone, and another for electromagnetic (EM) loop and geophone) were carried out near Kimpo Airport and at Kimje. The spectrum analyses were also performed to investigate the correlation of two pairs of time series; one for microphone and geophone, and another for EM loop and geophone. The sound waves measured with the microphone and the geophone are highly correlated. However, differences in the reponses are readily identifiable across 200 Hz; in the vicinity of 100 Hz, the spectral energy for geophone is 20 dB higher than that for microphone, and at near 500 Hz, the spectral energy for microphone is 30 dB higher than that for geophone. Overall, the spectral energy appears concentrated on the frequency window below 600 Hz for geophone. It contrasts with the observation of dominant frequency at the range of above 200 Hz for microphone. The wave forms of EM noise (due to an ACDC inverter) measured with EM loop and geophone are consistently and highly correlated each other. The power spectrum of the EM noise for EM loop shows that the spectral energies at odd harmonic frequencies of 60 Hz are higher than those at even harmonic frequencies of 60 Hz. It is compared to the power spectrum for geophone; the spectral energies at odd harmonics are nearly same as those at even harmonic frequencies.