• Journal of Microbiology and Biotechnology
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• v.29 no.4
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• pp.548-557
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• 2019

Staphylococcus species have a ubiquitous habitat in a wide range of foods, thus the ability to identify staphylococci at the species level is critical in the food industry. In this study, we performed rapid identification of Staphylococcus species using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS was evaluated for the identification of Staphylococcus reference strains (n = 19) and isolates (n = 96) from various foods with consideration for the impact of sample preparation methods and incubation period. Additionally, the spectra of isolated Staphylococcus strains were analyzed using principal component analysis (PCA) and a main spectra profile (MSP)-based dendrogram. MALDI-TOF MS accurately identified Staphylococcus reference strains and isolated strains: the highest performance was by the EX method (83.3~89.5% accuracy) at species level identification (EDT, 70.3~78.9% accuracy; DT, less than 46.3~63.2% accuracy) of 24-h cultured colonies. Identification results at the genus level were 100% accurate at EDT, EX sample preparation and 24-h incubation time. On the other hand, the DT method showed relatively low identification accuracy in all extraction methods and incubation times. The analyzed spectra and MSP-based dendrogram showed that the isolated Staphylococcus strains were characterized at the species level. The performance analysis of MALDI-TOF MS shows the method has the potential ability to discriminate between Staphylococcus species from foods in Korea. This study provides valuable information that MALDI-TOF MS can be applied to monitor microbial populations and pathogenic bacteria in the food industry thereby contributing to food safety.

• Smart Media Journal
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• v.2 no.4
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• pp.60-65
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• 2013

In this paper, we propose a high-resolution disparity map generation method using a low-resolution time-of-flight (TOF) depth camera and color camera. The TOF depth camera is efficient since it measures the range information of objects using the infra-red (IR) signal in real-time. It also quantizes the range information and provides the depth image. However, there are some problems of the TOF depth camera, such as noise and lens distortion. Moreover, the output resolution of the TOF depth camera is too small for 3D applications. Therefore, it is essential to not only reduce the noise and distortion but also enlarge the output resolution of the TOF depth image. Our proposed method generates a depth map for a color image using the TOF camera and the color camera simultaneously. We warp the depth value at each pixel to the color image position. The color image is segmented using the mean-shift segmentation method. We define a cost function that consists of color values and segmented color values. We apply a weighted average filter whose weighting factor is defined by the random walk probability using the defined cost function of the block. Experimental results show that the proposed method generates the depth map efficiently and we can reconstruct good virtual view images.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.49 no.5
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• pp.373-381
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• 2021

This paper details the result of flight experiment that examines performance of roll angle estimation algorithm of slowly rolling munition taking time delay of measurement into account when measurement comes in delayed fashion. As the measurement is passed through low pass filter for numerical stabilization and de-noising purpose which induces time delay, we design augmented state Kalman filter that incorporates distribution models of stochastic delay over time. Flight experiment was conducted to verify the algorithm at around 250m high AGL(Above Ground Level) conveying velocity of 28m/s from fixed-wing mother plane to the munition. Munition was made spun with respect to its roll axis using internal reaction wheel afterward. Numerical comparison of proposing method's roll estimation performance with that of commercial aerospace graded GPS/INS shows that proposed filter design can effectively compensate time delay of measurement.

• Journal of the Korean Society of Industry Convergence
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• v.26 no.5
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• pp.775-784
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• 2023

Drone images are being used more and more actively in the fields of surveying and spatial information, and are rapidly replacing existing aerial and satellite images. The technology of quickly acquiring real-time data at low cost and processing it is now being applied to actual industries beyond research. However, there are also problems encountered as this progresses. When high-resolution spatial information is acquired using a general 2D flight plan for a terrain with sever undulations, problems arise due to the difference in resolution of the data. In particular, when a low-altitude high-resolution image is taken using a drone in a mountainous or steep terrain, there may be a problem in image matching due to a resolution difference caused by terrain undulations. This problem occurs because a drone acquires data while flying on a 2D plane at a fixed altitude, just like conventional aerial photography. In order to acquire high-quality 3D data using a drone, the scale difference for the shooting distance should be considered. In addition, in order to obtain facade images of large structures, it is necessary to take images in 3D space. In this study, in order to improve the disadvantages of the 2D flight method, a 3D flight plan was established for the study area, and it was confirmed that high-quality 3D spatial information could be obtained in this way.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.1
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• pp.268-277
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• 2019

Flight test systems should monitor various conditions in real time during flight tests and take safety measures in an emergency. The importance of ensuring test safety increases in more complicated and wider test environments. Also, due to the transition of wartime operational authority, many guided missile systems must be developed simultaneously. Early deployment and budget reduction by shortening the development and T&E periods are also necessary. Consequently, the risk of flight tests under the circumstance of inefficient test resources is increasing. To address this deficiency, a flight test system model using SysML was proposed in this study. The method of designing and verifying the test system is based on the agile shift left testing methodology of advanced T&E labs and utilizing a system reference model in the aerospace field. Through modeling and simulation analysis, early identification and correction of faults resulting from inconsistent test requirements can mitigate the risk of delays during the T&E phase of flight tests. Also, because the flight test system model was constructed using SysML, it can be applied to test various guided missile systems.

• 제어로봇시스템학회:학술대회논문집
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• 2001.10a
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• pp.141.4-141
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• 2001

In this paper the Local Weighted Regression LWR technique is applied to the estimation of aircrcraft parameters. The method consists In improving the Local Weighted Regression LWR technique by adding a data Retention-and-Deletion RD strategy. The improvement comes with reduced computational effort since the two techniques can share their main computational procedures. The purpose of the study was to establish if the proposed algorithm could provide fast and reliable real-time estimations, with accuracy comparable to other well-known off-line identification schemes. The algorithm was tested using specific parameter estimation maneuvers and flight data of the NASA F/A-18 HARV. The results were compared with both the estimation obtained from ...

• The Journal of Korea Robotics Society
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• v.13 no.4
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• pp.230-236
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• 2018

3D depth perception has played an important role in robotics, and many sensory methods have also proposed for it. As a photodetector for 3D sensing, single photon avalanche diode (SPAD) is suggested due to sensitivity and accuracy. We have researched for applying a SPAD chip in our fusion system of time-of-fight (ToF) sensor and stereo camera. Our goal is to upsample of SPAD resolution using RGB stereo camera. Currently, we have 64 x 32 resolution SPAD ToF Sensor, even though there are higher resolution depth sensors such as Kinect V2 and Cube-Eye. This may be a weak point of our system, however we exploit this gap using a transition of idea. A convolution neural network (CNN) is designed to upsample our low resolution depth map using the data of the higher resolution depth as label data. Then, the upsampled depth data using CNN and stereo camera depth data are fused using semi-global matching (SGM) algorithm. We proposed simplified fusion method created for the embedded system.

• Korean Journal of Applied Biomechanics
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• v.27 no.3
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• pp.171-179
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• 2017

Objective: The purpose of this study was to perform a kinematic and kinetic analysis of double-under jump rope technique according to skill level and sex. Method: Participants comprised a skilled group of 16 (9 males, 7 females), and an unskilled group of 16 with 6 months or less of experience (9 males, 7 females). Five consecutive double-under successes were regarded as 1 trial, and all participants were asked to complete 3 successful trials. The data for these 3 trials were averaged and analyzed after collecting the stable third jump in each trial. The variables used in the analysis included phase duration, total duration, flight time, vertical toe height, stance width, vertical center of mass displacement, and right lower limb ankle, knee, and hip joint angles in the sagittal plane during all events. Results: The skilled group had a shorter phase and total duration and a shorter flight time than the unskilled group. The vertical center of mass displacement and ankle dorsiflexion angle were significantly smaller in the skilled group. The male group had a shorter phase duration than the female group. The vertical toe height was greater, the stance width was smaller, and the ankle and hip flexion angles were smaller in the male group. Conclusion: Variables that can be used to distinguish between skill levels are phase and total duration, flight time, vertical center of mass displacement, and ankle dorsiflexion angle. Differences between sexes in double-under jump rope technique may be related to lower limb flexion angle control.

• Mass Spectrometry Letters
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• v.5 no.1
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• pp.24-29
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• 2014

An advanced and reliable high performance liquid chromatography (HPLC)/ultraviolet detector (UV)/ion-trap time-of-flight (IT-TOF) mass spectrometry was developed for the simultaneous quantification of 19 marker compounds in Bang-poong-tong-sung-san (BPTS), a traditional oriental prescription. Various parameters affecting HPLC separation and IT-TOF detection were investigated, and optimized conditions were identified. The separation was achieved on a Capcell PAK C18 column ($1.5mm{\times}250mm$, $5{\mu}m$ particle size) using a gradient elution of acetonitrile and water containing 0.1% formic acid at a flow rate of 0.1 mL/min. The column temperature was maintained at $40^{\circ}C$ and the injection volume was $2{\mu}L$. IT-TOF system was equipped with an electrospray ion source (ESI) operating in positive or negative ion mode. The optimized electrospray ionization parameters were as follows: ion spray voltage, +4.5 kV (positive ion mode), or -3.5 kV (negative ion mode); drying gas ($N_2$), 1.5 L/min; heat block temperature, $200^{\circ}C$. Automatic $MS^n$ (n = 1~3) analyses were carried out to obtain structural information of analytes. Elemental compositions and their mass errors were calculated based on their accurate masses obtained from a formula predictor software. The marker compounds in BPTS were identified by comparisons between $MS^n$ spectra from standards and those from extracts. Moreover, the libraries of $MS^2$ and $MS^3$ spectra and accurate masses of parent and fragment ions for marker compounds were constructed. The developed method was successfully applied to the BPTS extracts and identified 17 out of 19 marker compounds in the BPTS extracts.

• Journal of the Korea Institute of Military Science and Technology
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• v.17 no.1
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• pp.90-95
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• 2014

The system design and the system performance analysis of 3D imaging laser radar system for the mapping purpose is addressed in this article. For the mapping, a push-bloom scanning method is utilized. The pulsed fiber laser with high pulse energy and high pulse repetition rate is used for the light source of laser radar system. The high sensitive linear mode InGaAs avalanche photo-diode is used for the laser receiver module. The time-of-flight of laser pulse from the laser to the receiver is calculated by using high speed FPGA based signal processing board. To reduce the walk error of laser pulse regardless of the intensity differences between pulses, the time of flight is measured from peak to peak of laser pulses. To get 3D image with a single pixel detector, Risley scanner which stirs the laser beam in an ellipsoidal pattern is used. The system laser energy budget characteristics is modeled using LADAR equation, from which the system performances such as the pulse detection probability, false alarm and etc. are analyzed and predicted. The test results of the system performances are acquired and compared with the predicted system performance. According to test results, all the system requirements are satisfied. The 3D image which was acquired by using the laser radar system is also presented in this article.