• 제목/요약/키워드: Time effect

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Establishment of a Method for the Analysis of Diarrhetic Shellfish Poisoning by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS를 이용한 설사성패류독소의 분석조건 확립)

  • Lee, Ka-Jeong;Suzuki, Toshiyuki;Kim, Poong-Ho;Oh, Eun-Gyoung;Song, Ki-Cheol;Kim, Ji-Hoe
    • Korean Journal of Food Science and Technology
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    • 제41권4호
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    • pp.458-463
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    • 2009
  • To establish and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the rapid and accurate quantitation of diarrhetic shellfish poisoning (DSP) toxins, we compared the results from different mobile phases and columns used for their analysis and collision energies for MS/MS experiments. Clear peaks of okadaic acid (OA) and dinophysistoxin-1 (DTX1) were obtained by using a mobile phase comprising aqueous acetonitrile containing 2 mM ammonium formate and 50 mM formic acid. The collision energies were optimized to facilitate the most sensitive detection for each toxin, namely, OA, DTX1, pectenotoxin-2 (PTX2), or yessotoxin (YTX). Further, the maximum ion response was obtained at a collision energy of 48 V for OA and DTX1. We compared the analytical performance of $C_8$ and $C_{18}$ columns. A wide range of toxins namely, OA, DTX1, PTX2, and YTX, except DTX3, were detected by both the columns. Although DTX3 was only detected by the $C_8$ column, we found that the $C_{18}$ column was also suitable for the quantitation of OA and DTX1 the toxins responsible for inducing diarrhea. The limit of detection of OA and DTX1 by the established LC-MS/MS conditions was 1 ng/g, and the limit of quantitation of the toxins under the same conditions was 3 ng/g. The process efficiencies were 91-118% for oysters (Crassostrea gigas) and 96-117% for mussels (Mytilus galloprovincialis) further, we observed no significant effect of matrix during the ionization process in LC-MS/MS. The comparison between mouse bioassay (MBA) and LC-MS/MS yielded varying results because low concentrations of OA and DTX1 were detected by LC-MS/MS in some shellfish samples, which provided positive results on MBA for DSP. The analysis time required by MBA for DSP analysis can be reduced by LC-MS/MS.

Erosion Control Effect by Soil ansi Vegetation Transition in Mountainous Area after Soil Erosion Measures were Initiated (토양 및 식생변화에 따른 토지 사방 공사의 효과에 관한 연구)

  • 이천용
    • Journal of the Korean Institute of Landscape Architecture
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    • 제14권2호
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    • pp.7-16
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    • 1986
  • This study was carried out to investigate the effects of such erosion control measures as sowing, planting and small earth structures on the soil and vegetation. In order to study the changes in soil and vegetation, 36 plots were surveyed from 1981 to 1982 in the large erosion control area which is restored last 20 years. The factors which were measured included vegetation coverage, tree growth, number of species, soil depth, soil consistancy, and Chemical properties of soil. The results were as follows; 1) Maximum coverage of the overstory and understory was attained 7 years after the initiation of erosion control. So the overstory need to be tended and pruned. 2) Diversity of species increased until age 6 after which it began to decrease. 3) In order of tree growth, black locust was the fastest, followed by siberian alder and pitch pine. The initial growth of black locust, though the best among the 3 tree stop., decreased rapidly year by year. At the same time, siberian alder and pitch pine grew well until 12 and 6 years after the initiation of erosion control respectively. 4) Fifty percent of the initially planted trees died within 8 yeard. The mortality of siberian alder occurred until the 20th year while the mortality of pitch pine stopped after 10 years. Thereafter 500 trees per hectare were maintained. 5) The soil depth in A and B horision increased by 2cm annually during 20 years. The soil consistency also decreased rapidly until 7th year. The physical soil properties of the rehabilitated areas were improved after the 14th year. 6) The soil pH tend to decrease from 5.3 during the first year to 5.1 during the twentieth year. 7) The organic matter and nitrogen content in the soil were increased by fertilization but after 20 years these nutrients are still deficient for normal tree growth. 8) The phosphorous content in the soil was high in the first year but the longer the period after the initiation of erosion control the lese the content of phosphorous. 9) The biomass of black locust was the highest and increased continuously. The biomass of siberian alder on the contrary decreased from the 15th year because the number of trees in this place was very low. The total biomass in the twentieth year after erosion control initiation was 105.7 ton per hectare.

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Clinical assessment of whitening efficacy and safety of in-office tooth whitening system containing 15% hydrogen peroxide with or without light activation (15% 과산화수소 함유 전문가용 치아 미백제의 광활성화 여부에 따른 미백효과 및 안전성에 관한 임상평가)

  • Noh, Young-Suk;Rho, Young-Jee;Yoo, Yeon-Jee;Lee, Hyang-Ok;Lim, Sang-Min;Kweon, Hyun-Jeong;Kim, Ye-Un;Park, Seong-Yeon;Yoon, Hee-Young;Lee, Jung-Hyun;Lee, Chan-Hee;Oh, So-Ram;Kum, Kee-Yeon
    • Restorative Dentistry and Endodontics
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    • 제36권4호
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    • pp.306-312
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    • 2011
  • Objectives: This clinical study evaluated the effect of light activation on the whitening efficacy and safety of in-office bleaching system containing 15% hydrogen peroxide gel. Materials and Methods: Thirty-three volunteers were randomly treated with (n = 17, experimental group) or without light activation (n = 16, control group), using Zoom2 white gel (15% $H_2O_2$, Discus Dental) for a total treatment time of 45 min. Visual and instrumental color measurements were obtained using Vitapan Classical shade guide and Shadepilot (DeguDent) at screening test, after bleaching, and 1 month and 3 month after bleaching. Data were analyzed using t-test, repeated measure ANOVA, and chi-squared test. Results: Zoom2 white gel produced significant shade changes in both experimental and control group when pre-treatment shade was compared with that after bleaching. However, shade difference between two groups was not statistically significant (p > 0.05). Tooth shade relapse was not detected at 3 months after bleaching. The incidence of transient tooth sensitivity was 39.4%, with being no differences between two groups. Conclusions: The application of light activation with Zoom2 white gel system neither achieved additional whitening effects nor showed more detrimental influences.

A Study on the Effect of Using Sentiment Lexicon in Opinion Classification (오피니언 분류의 감성사전 활용효과에 대한 연구)

  • Kim, Seungwoo;Kim, Namgyu
    • Journal of Intelligence and Information Systems
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    • 제20권1호
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    • pp.133-148
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    • 2014
  • Recently, with the advent of various information channels, the number of has continued to grow. The main cause of this phenomenon can be found in the significant increase of unstructured data, as the use of smart devices enables users to create data in the form of text, audio, images, and video. In various types of unstructured data, the user's opinion and a variety of information is clearly expressed in text data such as news, reports, papers, and various articles. Thus, active attempts have been made to create new value by analyzing these texts. The representative techniques used in text analysis are text mining and opinion mining. These share certain important characteristics; for example, they not only use text documents as input data, but also use many natural language processing techniques such as filtering and parsing. Therefore, opinion mining is usually recognized as a sub-concept of text mining, or, in many cases, the two terms are used interchangeably in the literature. Suppose that the purpose of a certain classification analysis is to predict a positive or negative opinion contained in some documents. If we focus on the classification process, the analysis can be regarded as a traditional text mining case. However, if we observe that the target of the analysis is a positive or negative opinion, the analysis can be regarded as a typical example of opinion mining. In other words, two methods (i.e., text mining and opinion mining) are available for opinion classification. Thus, in order to distinguish between the two, a precise definition of each method is needed. In this paper, we found that it is very difficult to distinguish between the two methods clearly with respect to the purpose of analysis and the type of results. We conclude that the most definitive criterion to distinguish text mining from opinion mining is whether an analysis utilizes any kind of sentiment lexicon. We first established two prediction models, one based on opinion mining and the other on text mining. Next, we compared the main processes used by the two prediction models. Finally, we compared their prediction accuracy. We then analyzed 2,000 movie reviews. The results revealed that the prediction model based on opinion mining showed higher average prediction accuracy compared to the text mining model. Moreover, in the lift chart generated by the opinion mining based model, the prediction accuracy for the documents with strong certainty was higher than that for the documents with weak certainty. Most of all, opinion mining has a meaningful advantage in that it can reduce learning time dramatically, because a sentiment lexicon generated once can be reused in a similar application domain. Additionally, the classification results can be clearly explained by using a sentiment lexicon. This study has two limitations. First, the results of the experiments cannot be generalized, mainly because the experiment is limited to a small number of movie reviews. Additionally, various parameters in the parsing and filtering steps of the text mining may have affected the accuracy of the prediction models. However, this research contributes a performance and comparison of text mining analysis and opinion mining analysis for opinion classification. In future research, a more precise evaluation of the two methods should be made through intensive experiments.

Influence of Glycerol Concentration, Freezing Rate and Thawing Rate on Survival of Canine Spermatozoa Frozen (개 정자의 동결보존에 있어서 Glycerol 농도, 동결 및 융해 속도가 정자의 생존율 및 운동성에 미치는 영향)

  • Lee, Je-Hyub;Park, Hyang;Park, Heum-Dae;Kim, Jae-Myung
    • Journal of Embryo Transfer
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    • 제18권3호
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    • pp.195-201
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    • 2003
  • This study was carried out to establish most suitable freezing condition, to evaluate the different condition of freezing and thawing rates on the viability and motility of frozen canine spermatozoa. The ejaculated semen was added to obtained 200∼400 ${\times}$ 10$^{6}$ /$m\ell$ with extender I and was cooled to 4$^{\circ}C$ over 30, 60 and 120 minutes. And then, semen was diluted with extender II containing 4, 6 and 8%(v/v) glycerol for 60 min, respectively and packaged in 0.5$m\ell$ straw, equilibrated far 30, 60 and 120 min at 4$^{\circ}C$ and cryopreserved in liquid nitrogen vapor at different distance(3, 5, 7 and 9 cm, respectively), plunged into nitrogen tank. Samples were thawed by placing straws into 27, 37, 47, 57$^{\circ}C$ water bath for 120, 20 and 12 sec, respectively. The results were as follows; 1. The survival and motility rate immediately post-thawing was significantly higher in samples frozen in 4% glycerol than 6 or 8% glycerol(P< 0.05). 2. According to equilibration time at 4$^{\circ}C$, the survival and motility rate immediately post-thawing was significantly higher in samples frozen after 60 min equilibration than 30 or 120 min equilibration(P<0.05). 3. Freezing in distance of 5 cm from liquid nitrogen yield better survival and motility rate than the others(3, 7 or 9 cm)(P<0.05). 4. The effect of thawing rate on sperm survival were higher when the thawing was done at 37$^{\circ}C$ for 120 sec(P<0.05).

Effects of Harvesting Methods on Properties of Cured-leaves in Aromatic Tobacco Production (향끽미종의 수확방법이 건조엽특성에 미치는 영향)

  • 이철환;조명조
    • KOREAN JOURNAL OF CROP SCIENCE
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    • 제34권2호
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    • pp.177-183
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    • 1989
  • Lower leaves of aromatic tobacco are also much lower in Quality than upper leaves. So feasibility test of no harvesting and curing of lower leaves was conducted under high planting density and high nitrogen conditions with conventional cultural system. Effect of harvesting time on yield and Quality were investigated under 2 nitrogen levels. Among harvesting methods of conventional harvest with priming under high planting density, no-harvest of first priming, removal of lower leaves which relevant to first prime stalk before maturity, no-harvest of first and second priming. no-harvesting or pruning of first prime stalk before maturity was best in yield, price and in crude income. The shortor the harvest period became, the lower the yield, price and contents of reducing sugar and nicotine became, but reverse in this trends with total nitrogen and protein nitrogen. So 6 or 8 days interval of harvest is most recommendable.

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Change of Growth and Nitrogen Uptake of Rice at the Paddy Field with Previous Upland Condition (논.밭윤환 복원논의 벼 생육특성 및 질소흡수량 변화)

  • Seo, Jong-Ho;Lee, Chung-Keun;Cho, Young-Son;Lee, Chun-Ki;Kim, Chung-Kon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • 제55권2호
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    • pp.98-104
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    • 2010
  • Excess nitrogen (N) uptake of rice, which could cause much lodging, disease and reduction of rice quality, could be occurred at the paddy field with previous upland condition at which much soil N could be mineralized by soil-drying effect. N fertilizers of 0, 3, and 6 kg N $10a^{-1}$ were applied to early-maturity rice, cultivar Joanbyeo at the paddy field of first and second year after upland condition, and rice growth and nitrogen uptake were investigated to know the increase of rice N uptake at the paddy field with previous upland condition for one-year. Total dry matter (DM) and N uptake of rice at the paddy field with previous upland condition increased more than continuous paddy field. Total DM and N uptake of rice at the paddy field with previous upland condition increased linearly to N fertilizer 6 kg $10a^{-1}$ at the paddy field owing to vigorous growth compared to continuous paddy field. Rice N uptake was higher at the paddy field of the first year than the second year after upland condition in considering N uptake at the plot of no N fertilizer. Vigorous growth at the paddy field with previous upland condition resulted in higher rice yield which was related with high panicle and spikelet, but much N fertilizer as much as 6 kg $10a^{-1}$ at the paddy field with previous upland condition resulted in higher lodging and protein content of brown and milled rice. Particularly, protein content of brown and milled rice increased more when the same N fertilizer was applied two times splitly at transplanting and panicle initiation stage than when N fertilizer was applied one time at transplanting as basal N. N application with under 3 kg $10a^{-1}$ as only basal N was recommended at the paddy field with previous upland condition to obtain high quality rice without lodging.

Computing the Dosage and Analysing the Effect of Optimal Rechlorination for Adequate Residual Chlorine in Water Distribution System (배.급수관망의 잔류염소 확보를 위한 적정 재염소 주입량 산정 및 효과분석)

  • Kim, Do-Hwan;Lee, Doo-Jin;Kim, Kyoung-Pil;Bae, Chul-Ho;Joo, Hye-Eun
    • Journal of Korean Society of Environmental Engineers
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    • 제32권10호
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    • pp.916-927
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    • 2010
  • In general water treatment process, the disinfection process by chlorine is used to prevent water borne disease and microbial regrowth in water distribution system. Because chlorines were reacted with organic matter, carcinogens such as disinfection by-products (DBPs) were produced in drinking water. Therefore, a suitable injection of chlorine is need to decrease DBPs. Rechlorination in water pipelines or reservoirs are recently increased to secure the residual chlorine in the end of water pipelines. EPANET 2.0 developed by the U.S. Environmental Protection Agency (EPA) is used to compute the optimal chlorine injection in water treatment plant and to predict the dosage of rechlorination into water distribution system. The bulk decay constant ($k_{bulk}$) was drawn by bottle test and the wall decay constant ($k_{wall}$) was derived from using systermatic analysis method for water quality modeling in target region. In order to predict water quality based on hydraulic analysis model, residual chlorine concentration was forecasted in water distribution system. The formation of DBPs such as trihalomethanes (THMs) was verified with chlorine dosage in lab-scale test. The bulk decay constant ($k_{bulk}$) was rapidly decreased with increasing temperature in the early time. In the case of 25 degrees celsius, the bulk decay constant ($k_{bulk}$) decreased over half after 25 hours later. In this study, there were able to calculate about optimal rechlorine dosage and select on profitable sites in the network map.

Examination for Efficiency of Groundwater Artificial Recharge in Alluvial Aquifer Near Nakdong River of Changweon Area, Korea (창원지역 낙동강 하천수와 주변 충적층을 이용한 지하수 인공함양의 효율성 평가)

  • Moon, Sang-Ho;Ha, Kyoochul;Kim, Yongcheol;Koh, Dong-Chan;Yoon, Heesung
    • Economic and Environmental Geology
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    • 제47권6호
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    • pp.611-623
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    • 2014
  • The alluvial aquifer, widely developed near the four major rivers such as Nakdong River, can be used effectively for groundwater artificial recharge and is expected to be the future water resources in Korea. This study is aimed at examining the impact of repeatedly injected river water into the riverside alluvial aquifer on injection rate or efficiency in its system at Changweon area. For this, injection tests were performed two times, first on June 19 and second on September 25 through October 9, 2013, and the mixing ratios of river water to groundwater were used as the tool to compare the efficiency of injection. The mixing ratios were evaluated by using electrical conductivities of injected river water (average $EC=303{\mu}S/cm$) and groundwater ($EC{\fallingdotseq}6,000{\mu}S/cm$) measured at 20 m depth of four observation wells installed 10 m apart from each injection well. The result shows the remarkable differences on two respects. First, in some observation well, detection time for incipient injection effect during $2^{nd}$ injection test was shown to be much slower than that of $1^{st}$ injection test. Second, the hourly increasing rate of mixing ratios in $2^{nd}$ test was revealed to be reduced much more than that of $1^{st}$ test. This means that the efficiency of injection was badly deteriorated by only 1,210 minute injection work. Therefore, injection water needs to be adequately treated beforehand and repeated pumping work and/or resting phase is needed afterwards. To a certain extent, the improvement of water quality in saline aquifer was verified in this system by injection tests.

A Study on E-mail Campaigns and Feedback Analysis as Marketing Tools of Internet Fashion Shopping Malls - With Focus on Specialized Fashion Shopping Malls - (인터넷 패션쇼핑몰의 이메일 마케팅 활용과 반응 - 패션 전문몰을 중심으로 -)

  • Han, Ji-Sook
    • Archives of design research
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    • 제19권2호
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    • pp.53-62
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    • 2006
  • E-mail has indeed developed from 'a means of instant communication' to an indispensable part of online marketing. Therefore, companies need to implement consistent customer management. Communication with customers and marketing through e-mail is a powerful way of communication and adapting one-to-one marketing strategies to customer trends, habits and taste preferences. Since setting accurate targets is especially important in the fashion industry, e-mail marketing is the most effective way to communicate with customers and one-to-one marketing constitutes a very important strategy. In this study, I will analyze this powerful one-on-one marketing tool, particularly actual e-mail messages sent by an Internet Shopping Mall from June 12 to July 30, 2005, examine the effect of these messages on sales growth and analyze actual feedback received. Regarding e-mail read rates broken down by age and gender, 1 found that females in their late twenties recorded the highest rate at 21.66% and their contribution to sales growth was recorded at 3.5% From actual sales records, found that 28.10% of total sales were attributable to people in their late twenties, showing that the age group that reads e-mails the most also buys the most. Regarding feedback by e-mail title, e-mails from the 'Casual' category seemed to be the most effective, in that most of these e-mails were read. Also, messages sent on Tuesdays were read the most, according to the feedback analysis by weekday. Section e-mails were read more often than regular e-mails. Regarding the view rate according to the time e-mails were sent, messages sent to females in their late twenties at two o'clock in the afternoon were read by 20.93% of recipients, recording the highest read rate. By offering informative content and practical tips, visitors will be attracted to the site and generate site traffic. Therefore, we can conclude that sending e-mail messages can greatly contribute to sales growth and e-mail marketing is very effective. Also, in order to make e-mail campaigns more effective and improve marketing results, we need to analyze actual results and apply our findings in future e-mail campaigns. With this, we get successful marketing results.

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