• Asian Pacific Journal of Cancer Prevention
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• v.16 no.7
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• pp.3061-3065
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• 2015

Background: Rac3, a member of the Rac family of small guanosine triphosphatases (GTPases), regulates a variety of cell functions, including the organization of the cytoskeleton, cell migration, and invasion. Overexpression of Rac3 has been reported in several human cancers. However, the role of Rac3 in lung cancer (LC) has not been determined in detail. The purpose of this study was to investigate the effect of silencing of Rac3 expression in human LC cells and the consequences for cell survival. Materials and Methods: Lentivirus small hairpin RNA (shRNA) interference techniques were utilized to knock down the Rac3 gene. Gene and protein expression was quantified by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. LC cell apoptosis was examined by annexin V-APC /propidium iodide staining. Results: Efficient silencing of Rac3 strongly inhibited A549 cell proliferation and colony formation ability, and significantly decreased tumor growth. Moreover, flow cytometry analysis showed that knockdown of Rac3 led to G2/M phase cell cycle arrest as well as an excess accumulation of cells in the G1 and S phase. Conclusions: Thus, functional analysis using shRNAs revealed a critical role for Rac3 in the tumor growth of LC cells. shRNA silencing of Rac3 could provide an effective strategy to treat LC.

• Smart Structures and Systems
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• v.23 no.6
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• pp.653-667
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• 2019

Using cross-ties to connect cables together when forming a cable network is regarded as an efficient method of mitigating cable vibrations. Cross-ties have been extended and fixed on bridge decks or towers in some engineering applications. However, the dynamics of this kind of system need to be further studied, and the effects of extending cross-links to bridge decks/towers on the modal response of the system should be assessed in detail. In this paper, a system of two cables connected by an inter-supported cross-link with another lower cross-link extended to the ground is proposed and analyzed. The characteristic equation of the system is derived, and some limiting solutions in closed form of the system are derived. Roots of cable system with special configurations are also discussed, attention being given to the case when the two cables are identical. A predictable mode behavior was found when the stiffness of inter-connection cross-link and the cross-link extended to the ground were the same. The vector of mode energy distribution and the degree of mode localization index are proposed so as to distinguish global and local modes. The change of mode behaviors is further discussed in the case when the two cables are not identical. Effects of cross-link stiffness, cross-link location, mass-tension ratio, cable length ratio and frequency ratio on $1^{st}$ mode frequency and mode shape are addressed.

• Architectural research
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• v.21 no.3
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• pp.59-67
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• 2019

Although many stadiums around the world have incorporated various profitable facilities, many are making conventional and cursory decisions without deep consideration of the interrelationships of different programs. This paper investigates cases in which new programs such as a hotel, a youth hostel, and a dormitory have been introduced into stadiums, showing different results. In the first part of this paper, the theoretical precedents of program combination are studied. Although Bernard Tschumi's notion of the combination of different programs presented its prophetic probability, this paper discern different approaches addressing program mixture, engendering eventually a productive modification of his notion based on the empirical observation of the cases. This paper classifies the manner of programmatic combination into complexity and hybridity, arguing that the latter transforms the spaces and gives rise to unexpected synergy, while the former merely assembles different programs. The second part compares the spaces of complexity and hybridity in stadiums. Through the plan and the section analysis of the spatial structure and the interrelationship of programs, this section reveals how the two different ways of the program combinations have had different results. In hybrid stadiums, programs are not simply gathered, but directly connected and intertwined. In the third part, the nature of the spatial transformation in the hybrid space is researched in detail. In the hybrid stadiums, the collision of the different programs changes the conception of the programs themselves and their related spaces. Hotels and stadiums are not what they once were, provoking unanticipated situations. These transformed spaces not only suggest a method of reutilizing of disused urban spaces, but also of meaningful and communicative program mixture, diversifying and vitalizing a city, not isolated islands of discrepancy. The ultimate purpose of this paper is clarifying the programmatic hybrid paradigm, surpassing complexity through the analysis of the stadium cases and illuminating the manner by which the hybridity breaks the typical tie between the program and space, to cause transformed situations.

• Steel and Composite Structures
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• v.32 no.6
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• pp.807-819
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• 2019

In recent earthquakes, the failure of ceiling systems has been one of the most widely reported damage and the major cause of functionality interruption in some buildings. In an effort to mitigate this damage, some scholars have studied a series of ceiling systems including plaster ceilings and mineral wool ceilings. But few studies have involved the backdrop metal ceiling used in some important constructions with higher rigidity and frequency such as the main control area of nuclear power plants. Therefore, in order to evaluate its seismic performance, a full-scale backdrop metal ceiling system, including steel runners and metal panels, was designed, fabricated and installed in a steel frame in this study. And the backdrop metal ceiling system with two perimeter attachments variants was tested: (i) the ends of the runners were connected with the angle steel to form an effective lateral constraint around the backdrop metal ceiling, (ii) the perimeter attachments of the main runner were retained, but the perimeter attachments of the cross runner were removed. In the experiments, different damage of the backdrop metal ceiling system was observed in detail under various earthquakes. Results showed that the backdrop metal ceiling had good integrity and excellent seismic performance. And the perimeter attachments of the cross runner had an adverse effect on the seismic performance of the backdrop metal ceiling under earthquakes. Meanwhile, a series of seismic construction measures and several suggestions that need to be paid attention were proposed in the text so that the backdrop metal ceiling can be better applied in the main control area of nuclear power plants and other important engineering projects.

• Journal of the Korea institute for structural maintenance and inspection
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• v.24 no.4
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• pp.72-80
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• 2020

The purpose of this study is to secure the same or more structural performance and constructability for the details of hooks cross-constructed at 135 degrees used as external-ties standard detail in RC columns, therefore, to the purpose of improving constructability, the clip-type binding implement was suggested and A total of 28 pull-out specimens were prepared with the parameters of concrete compressive strength and clip-embeded length, clip installation location to examine the anchorage behavior of the clip-type binding implement. The experiment was carried out. The results of the experiment confirmed that the anchorage strength of the clip-type binding implement was higher than the details of hooks cross-constructed at 135-degree regardless of the diameter of tie and concrete strength, embeded clip length, clip installation. and The 90-degree end hook with clip-type binding implement was showed a similar an anchorage behavior of 135-degree end-hooked transverse reinforcement, consequently, The 90-degree end hooked with clip-type binding implement is evaluated to be the same anchorage behavior and performance as standard 135-degree end hook detail.

• Journal of the Korea Fashion and Costume Design Association
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• v.8 no.1
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• pp.81-96
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• 2006

This study aimed to investigate the sociocultural phenomena which affected to clothing change in 1650-1900 and to examine the features of men's necktie in the western art. For this study, information about historic neckwear is obtained from the visual evidence of painted or engraved portraits, contemporary written sources. The covered area of this study was Europe mainly England and France. For this process, research steps were as follows : 1. Investigating the sociocultural phenomena which affect to clothing change through 1650-1900 and analysis of men's clothing and appearance. 2. Finding the changing characteristics of neckties including it's different types, shapes, knots, colors and materials and analyzing similarities and differences of neckties by the times. 3. Examining necktie as one of important and representative icons of male gender identity. The results of the study was as follows: 1. In 1660-1900, men's fashion was simplified in color and detail due to the influence of practical Puritanism and matured civil culture. And British men's fashion spreaded throughout whole European countries and get popularity. 2. In 1650-1720, there were band, cravat and steinkirk. And expecially cravatier, a expert custodian of cravats, was appeared in that period. In 1720-1800, there were stock, solitaire and cravat. In 1800-1850, neckwear were popularized and got various sizes and types up to shape and size of chemise collars. Black stock, scarf cravat and shawl cravat were popular and terms of 'necktie' were used for an certain neckcloth shape. And abundant literature for necktie were published thanks to the development of printing technology in that period. In 1851-1900, the leady-made neckties were spreaded and there were changes in shape, length, knot of necktie up to V-zone formed with shirt's collars and vest types. Neckwear was gradually evolving through four distinct styles, bow tie, scarf or neckerchief, Ascot and four-in-hand. 3. After the mid-l7th Century, as civil culture matured and splendid and extravagant colorful men's wear disappeared, British men's fashion spreaded throughout whole European countries and got popularity. The necktie become an essential ornamental accessory of men's fashion and one of important and representative icons of male gender identity.

• Journal of the Korea Concrete Institute
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• v.21 no.3
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• pp.347-358
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• 2009

Recently, it is getting into a good situation for the flat-plate slab system to be applied. The flat-plate slab without beam, however, is often too weak to control deflection properly compared to other typical slab-beam structures, for which the post-tension method is generally regarded as one of best solutions. The post-tension (PT) method can effectively control deflection without increase of slab thickness. Despite this good advantage, however, the application of PT method has been very limited due to cost increase, technical problems, and lack of experiences. Therefore, in order to reduce difficulties on applying full PT method under the current domestic circumstances and to enhance constructability of PT system, this research proposed the partial PT method with top jacking anchorage applied in a part of span as need. For the top jacking anchorage system, the efficiency of deflection control shall be considered in detail because it can vary widely depending on the location of anchorage that can be placed anywhere as need, and tensile stresses induced at back of the anchorage zone also shall be examined. Therefore, in this study, analysis were performed on the efficiency of deflection control depending on the location of anchorage and on tensile stresses or forces using finite element method and strut and tie model in the proposed top jacking anchorage system. The proposed jacking system were also applied to the floor slabs at a construction site to investigate its applicability and the analysis results of slab behavior were compared to the measured values obtained from the PT slab constructed by the partial PT method. The result of this study indicates that the partial PT method can be very efficiently applied with little cost increase to control deflection and tensile stresses in the region as a need basis where problem exists.