• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.3061-3065
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• 2015

Background: Rac3, a member of the Rac family of small guanosine triphosphatases (GTPases), regulates a variety of cell functions, including the organization of the cytoskeleton, cell migration, and invasion. Overexpression of Rac3 has been reported in several human cancers. However, the role of Rac3 in lung cancer (LC) has not been determined in detail. The purpose of this study was to investigate the effect of silencing of Rac3 expression in human LC cells and the consequences for cell survival. Materials and Methods: Lentivirus small hairpin RNA (shRNA) interference techniques were utilized to knock down the Rac3 gene. Gene and protein expression was quantified by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. LC cell apoptosis was examined by annexin V-APC /propidium iodide staining. Results: Efficient silencing of Rac3 strongly inhibited A549 cell proliferation and colony formation ability, and significantly decreased tumor growth. Moreover, flow cytometry analysis showed that knockdown of Rac3 led to G2/M phase cell cycle arrest as well as an excess accumulation of cells in the G1 and S phase. Conclusions: Thus, functional analysis using shRNAs revealed a critical role for Rac3 in the tumor growth of LC cells. shRNA silencing of Rac3 could provide an effective strategy to treat LC.

• Smart Structures and Systems
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• 제23권6호
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• pp.653-667
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• 2019

Using cross-ties to connect cables together when forming a cable network is regarded as an efficient method of mitigating cable vibrations. Cross-ties have been extended and fixed on bridge decks or towers in some engineering applications. However, the dynamics of this kind of system need to be further studied, and the effects of extending cross-links to bridge decks/towers on the modal response of the system should be assessed in detail. In this paper, a system of two cables connected by an inter-supported cross-link with another lower cross-link extended to the ground is proposed and analyzed. The characteristic equation of the system is derived, and some limiting solutions in closed form of the system are derived. Roots of cable system with special configurations are also discussed, attention being given to the case when the two cables are identical. A predictable mode behavior was found when the stiffness of inter-connection cross-link and the cross-link extended to the ground were the same. The vector of mode energy distribution and the degree of mode localization index are proposed so as to distinguish global and local modes. The change of mode behaviors is further discussed in the case when the two cables are not identical. Effects of cross-link stiffness, cross-link location, mass-tension ratio, cable length ratio and frequency ratio on $1^{st}$ mode frequency and mode shape are addressed.

• Architectural research
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• 제21권3호
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• pp.59-67
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• 2019

Although many stadiums around the world have incorporated various profitable facilities, many are making conventional and cursory decisions without deep consideration of the interrelationships of different programs. This paper investigates cases in which new programs such as a hotel, a youth hostel, and a dormitory have been introduced into stadiums, showing different results. In the first part of this paper, the theoretical precedents of program combination are studied. Although Bernard Tschumi's notion of the combination of different programs presented its prophetic probability, this paper discern different approaches addressing program mixture, engendering eventually a productive modification of his notion based on the empirical observation of the cases. This paper classifies the manner of programmatic combination into complexity and hybridity, arguing that the latter transforms the spaces and gives rise to unexpected synergy, while the former merely assembles different programs. The second part compares the spaces of complexity and hybridity in stadiums. Through the plan and the section analysis of the spatial structure and the interrelationship of programs, this section reveals how the two different ways of the program combinations have had different results. In hybrid stadiums, programs are not simply gathered, but directly connected and intertwined. In the third part, the nature of the spatial transformation in the hybrid space is researched in detail. In the hybrid stadiums, the collision of the different programs changes the conception of the programs themselves and their related spaces. Hotels and stadiums are not what they once were, provoking unanticipated situations. These transformed spaces not only suggest a method of reutilizing of disused urban spaces, but also of meaningful and communicative program mixture, diversifying and vitalizing a city, not isolated islands of discrepancy. The ultimate purpose of this paper is clarifying the programmatic hybrid paradigm, surpassing complexity through the analysis of the stadium cases and illuminating the manner by which the hybridity breaks the typical tie between the program and space, to cause transformed situations.

• Steel and Composite Structures
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• 제32권6호
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• pp.807-819
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• 2019

In recent earthquakes, the failure of ceiling systems has been one of the most widely reported damage and the major cause of functionality interruption in some buildings. In an effort to mitigate this damage, some scholars have studied a series of ceiling systems including plaster ceilings and mineral wool ceilings. But few studies have involved the backdrop metal ceiling used in some important constructions with higher rigidity and frequency such as the main control area of nuclear power plants. Therefore, in order to evaluate its seismic performance, a full-scale backdrop metal ceiling system, including steel runners and metal panels, was designed, fabricated and installed in a steel frame in this study. And the backdrop metal ceiling system with two perimeter attachments variants was tested: (i) the ends of the runners were connected with the angle steel to form an effective lateral constraint around the backdrop metal ceiling, (ii) the perimeter attachments of the main runner were retained, but the perimeter attachments of the cross runner were removed. In the experiments, different damage of the backdrop metal ceiling system was observed in detail under various earthquakes. Results showed that the backdrop metal ceiling had good integrity and excellent seismic performance. And the perimeter attachments of the cross runner had an adverse effect on the seismic performance of the backdrop metal ceiling under earthquakes. Meanwhile, a series of seismic construction measures and several suggestions that need to be paid attention were proposed in the text so that the backdrop metal ceiling can be better applied in the main control area of nuclear power plants and other important engineering projects.

• 한국구조물진단유지관리공학회 논문집
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• 제24권4호
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• pp.72-80
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• 2020

이 연구의 목적은 철근콘크리트 기둥에서 외부띠철근 상세로 이용되는 갈고리 양단 135도 교차 시공 상세에 대해, 대등한 구조적 성능을 확보하면서, 동시에 시공성을 개선할 목적으로 클립형 연결장치로 띠철근 갈고리 보강하는 방법을 제시하였다. 제안한 클립형 연결장치로 결속한 띠철근의 정착거동 및 강도를 파악하기 위해 콘크리트 압축강도, 클립형 연결장치의 묻힘길이, 설치 위치를 주요 변수로 28개 인발 실험체를 제작하여 실험하였다. 실험결과, 정착강도는 콘크리트 압축강도, 띠철근 직경, 클립의 묻힘길이, 설치 위치에 관계없이 클립형 연결장치로 결속하는 경우, 표준 갈고리 상세보다 모두 높게 나타났고 표준 갈고리 상세의 정착거동과 유사한 거동을 보여주었다. 그러므로, 90도 갈고리에 클립형 연결장치로 결속하는 상세는 표준 갈고리 상세와 대등한 정착거동 및 성능을 발휘할 수 있는 것으로 평가할 수 있다.

• 한국의상디자인학회지
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• 제8권1호
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• pp.81-96
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• 2006

This study aimed to investigate the sociocultural phenomena which affected to clothing change in 1650-1900 and to examine the features of men's necktie in the western art. For this study, information about historic neckwear is obtained from the visual evidence of painted or engraved portraits, contemporary written sources. The covered area of this study was Europe mainly England and France. For this process, research steps were as follows : 1. Investigating the sociocultural phenomena which affect to clothing change through 1650-1900 and analysis of men's clothing and appearance. 2. Finding the changing characteristics of neckties including it's different types, shapes, knots, colors and materials and analyzing similarities and differences of neckties by the times. 3. Examining necktie as one of important and representative icons of male gender identity. The results of the study was as follows: 1. In 1660-1900, men's fashion was simplified in color and detail due to the influence of practical Puritanism and matured civil culture. And British men's fashion spreaded throughout whole European countries and get popularity. 2. In 1650-1720, there were band, cravat and steinkirk. And expecially cravatier, a expert custodian of cravats, was appeared in that period. In 1720-1800, there were stock, solitaire and cravat. In 1800-1850, neckwear were popularized and got various sizes and types up to shape and size of chemise collars. Black stock, scarf cravat and shawl cravat were popular and terms of 'necktie' were used for an certain neckcloth shape. And abundant literature for necktie were published thanks to the development of printing technology in that period. In 1851-1900, the leady-made neckties were spreaded and there were changes in shape, length, knot of necktie up to V-zone formed with shirt's collars and vest types. Neckwear was gradually evolving through four distinct styles, bow tie, scarf or neckerchief, Ascot and four-in-hand. 3. After the mid-l7th Century, as civil culture matured and splendid and extravagant colorful men's wear disappeared, British men's fashion spreaded throughout whole European countries and got popularity. The necktie become an essential ornamental accessory of men's fashion and one of important and representative icons of male gender identity.

• 콘크리트학회논문집
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• 제21권3호
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• pp.347-358
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• 2009

최근에 들어서 국내에서도 무량판 구조 (flat plate slab)가 적용될 수 있는 여건이 마련되어가고 있다. 그러나 무량판 구조는 보가 없는 구조이기 때문에 일반적인 라멘구조형식에 비하여 처짐 제어가 어려우며, 포스트텐션 (post-tension) 공법은 이러한 문제점을 극복하기 위한 매우 효율적인 방법이다. 포스트텐션 구조를 적용하면 슬래브 두께의 증가 없이도 효율적으로 슬래브의 처짐을 제어 할 수 있다. 그러나, 이러한 장점에도 불구하고, 국내에서 포스트-텐션의 적용은 비용증가, 기술적인 문제, 경험부족 등으로 인하여 그 적용이 매우 제한적이다. 따라서 전구간 포스트텐션 (full-PT)에 대한 부담을 줄이고 나아가 기존 포스트텐션 구조형식의 시공성을 향상하기 위하여 본 연구에서는 슬래브 내부에서 긴장작업을 수행할 수 있는 상향긴장식 부분PT공법을 제안하였다. 이러한 상향긴장방식은 정착구를 필요에 따라 다양한 위치에 설치할 수 있기 때문에 처짐 효율에 대한 분석이 필요하며, 정착구 후면에서 인장응력을 야기시키는 문제점이 있다. 따라서, 본 연구에서는 상향긴장식 부분PT공법 적용할 때 정착구 위치에 따른 처짐효율분석, 유한요소해석 및 스트럿-타이를 이용한 인장응력 또는 인장력 해석 등을 실시하였으며, 상향긴장식 부분PT공법을 실제로 현장에 적용하고, 계측결과를 해석결과와 비교 분석함으로써 현장적용성을 알아보았다. 상향긴장식 부분PT공법은 처짐 또는 인장응력이 크게 발생되는 부분 구간에만 선택적으로 적용하면 적은 비용증가로 큰 처짐효율을 얻어낼 수 있음을 확인하였다.