• Journal of Microbiology and Biotechnology
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• v.28 no.11
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• pp.1916-1927
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• 2018

Corynebacterium glutamicum is an excellent platform for the production of amino acids, and is widely used in the fermentation industry. Most industrial strains are traditionally obtained by repeated processes of random mutation and selection, but the genotype of these strains is often unclear owing to the absence of genomic information. As such, it is difficult to improve the growth and amino acid production of these strains via metabolic engineering. In this study, we generated a complete genome map of an industrial L-valine-producing strain, C. glutamicum XV. In order to establish the relationship between genotypes and physiological characteristics, a comparative genomic analysis was performed to explore the core genome, structural variations, and gene mutations referring to an industrial L-leucine-producing strain, C. glutamicum CP, and the widely used C. glutamicum ATCC 13032. The results indicate that a 36,349 bp repeat sequence in the CP genome contained an additional copy each of lrp and brnFE genes, which benefited the export of L-leucine. However, in XV, the kgd and panB genes were disrupted by nucleotide insertion, which increase the availability of precursors to synthesize L-valine. Moreover, the specific amino acid substitutions in key enzymes increased their activities. Additionally, a novel strategy is proposed to remodel central carbon metabolism and reduce pyruvate consumption without having a negative impact on cell growth by introducing the CP-derived mutant $H^+$/citrate symporter. These results further our understanding regarding the metabolic networks in these strains and help to elucidate the influence of different genotypes on these processes.

• Animal Bioscience
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• v.34 no.12
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• pp.1940-1950
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• 2021

Objective: The objective of this study was to explore the effects of maize straw treated with calcium oxide (CaO) and various moisture, on the composition and molecular structure of the fiber, and gas production by fermentation in an in vitro rumen environment. Methods: The experiment used 4×3 Factorial treatment. Maize straws were treated with 4 concentrations of CaO (0%, 3%, 5%, and 7% of dry straw weight) and 3 moisture contents (40%, 50%, and 60%). Scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray fluorescence spectroscopy were employed to measure the surface texture, secondary molecular structure of carbohydrate, and calcium (Ca) content of the maize straw, respectively. The correlation of secondary molecular structures and fiber components of maize straw were analyzed by CORR procedure of SAS 9.2. In vitro rumen fermentation was performed for 6, 12, 24, 48, and 72 h to measure gas production. Results: Overall, the moisture factor had no obvious effect on the experimental results. Neutral detergent fiber (NDF), acid detergent fiber, acid detergent lignin, hemicellulose and cellulose contents decreased (p<0.05) with increasing concentrations of CaO treatment. Surface and secondary molecular structure of maize straw were affected by various CaO and moisture treatments. NDF had positive correlation (p<0.01) with Cell-H (H, height), Cell-A (A, area), CHO-2-H. Hemicellulose had positive correlation (p<0.01) with Lignin-H, Lignin-A, Cell-H, Cell-A. Ca content of maize straw increased as the concentration of CaO was increased (p<0.01). Gas production was highest in the group treated with 7% CaO. Conclusion: CaO can adhere to the surface of the maize straw, and then improve the digestibility of the maize straw in ruminants by modifying the structure of lignocellulose and facilitating the maize straw for microbial degradation.

• Journal of Electrical Engineering and Technology
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• v.10 no.1
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• pp.251-260
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• 2015

The main purpose of this paper is to describe a DTC (direct torque control) method for four-switch brushless dc motor (BLDCM) drive. In the method, a novel voltage space vector modulation scheme, an optimal switching table, and a flux observation method are proposed. Eight voltage vectors are summarized, which are selected to control BLDCM in SVPWM pattern, and an optimal switching table is proposed to improve the torque distortion caused by midpoint current of the split capacitors. Unlike conventional flux observers, this observer does not require speed adaptation and is not susceptible to speed estimation errors, especially, at low speed. Global asymptotic stability of the flux observer is guaranteed by the Lyapunov stability analysis. DC-offset effects are mitigated by introducing a PI component in the observer gains. This method alleviates the undesired current and torque distortion which is caused by uncontrollable phase. The correctness and feasibility of the method are proved by simulation and experimental results.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.4
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• pp.2607-2610
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• 2013

Malignant glioma, also known as brain cancer, is the most common intracranial tumor, having an extremely high mortality and recurrence rate. The survival rate of the affected patients is very low and treatment is difficult. Hence, growth inhibition of glioma has become a hot topic in the study of brain cancer treatment. Among the various isothiocyanate compounds, it has been confirmed that benzyl isothiocyanate (BITC) can inhibit the growth of a variety of tumors, including leukemia, glioma and lung cancer, both inside and outside the body. This study explored inhibitory effects of BITC on human glioma U87MG cells, as well as potential mechanisms. It was found that BITC could inhibit proliferation, induce apoptosis and arrest cell cycling of U87MG cells. In addition, it inhibited the expression of SOD and GSH, and caused oxidative stress to tumor cells. Therefore, it is believed that BITC can inhibit the growth of U87MG cells outside the body. Its mechanism may be related to the fact that BITC can cause oxidative stress to tumor cells.

• Steel and Composite Structures
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• v.18 no.4
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• pp.947-970
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• 2015

An equivalent friction element is proposed to simulate the friction in cable-strut joints. Equivalent stiffness matrixes and load vectors of the friction element are derived and are unified into patterns for FEM by defining a virtual node specially to store internal forces. Three approaches are described to verify the rationality of the new equivalent friction element: applying the new element in a cable-roller model, and numerical solutions match well with experimental results; applying the element in a continuous sliding cable model, and theoretical values, numerical and experimental results are compared; and the last is applying it in truss string structures, whose results indicate that there would be a great error if the cable of cable supported structures is simulated with discontinuous cable model which is usually adopted in traditional finite element analysis, and that the prestress loss resulted from the friction in cable-strut joints would have adverse effect on the mechanical performance of cable supported structures.

• Journal of Power Electronics
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• v.17 no.2
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• pp.368-379
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• 2017

In this paper, a direct torque control algorithm with novel duty cycle-based modulation is proposed for permanent magnet synchronous motor drives fed by neutral-point clamped three-level inverters. Compared with the standard DTC, the proposed algorithm can suppress steady-state torque ripples as well as ensure neutral-point potential balance and smooth vector switching. A unified torque/flux evaluation table with multiple voltage vectors and precise control levels is established and used in this method. This table can be used to evaluate the effects of duty-cycle vectors on torque and flux directly, and the elements of the table are independent of the motor parameters. Consequently, a high number of appropriate voltage vectors and their corresponding duty cycles can be selected as candidate vectors to reduce torque ripples by looking up the table. Furthermore, small vectors are incorporated into the table to ensure the neutral-point potential balance with the numerous candidate vectors. The feasibility and effectiveness of the proposed algorithm are verified by both simulations and experiments.

• Journal of Electrical Engineering and Technology
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• v.11 no.2
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• pp.274-284
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• 2016

The increasing penetration of distributed energy resources on the distribution networks have brought a number of technical impacts where voltage and thermal variations have been identified as the dominant effects. Active network management in distribution networks aims to integrate distributed energy resources with flexible network management so that distributed energy resources are organized to make better use of existing capacity and infrastructure. This paper propose active solutions which aims to solve the voltage and thermal issues in a distributed manner utilizing a collaborative approach. The proposed algorithms have been fully tested on a distribution network with distributed generation units.

• Journal of Electrical Engineering and Technology
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• v.10 no.6
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• pp.2384-2392
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• 2015

The technical development and practical applications of big-data for health is one hot topic under the banner of big-data. Big-data medical image fusion is one of key problems. A new fusion approach with coding based on Spherical Coordinate Domain (SCD) in Wireless Sensor Network (WSN) for big-data medical image is proposed in this paper. In this approach, the three high-frequency coefficients in wavelet domain of medical image are pre-processed. This pre-processing strategy can reduce the redundant ratio of big-data medical image. Firstly, the high-frequency coefficients are transformed to the spherical coordinate domain to reduce the correlation in the same scale. Then, a multi-scale model product (MSMP) is used to control the shrinkage function so as to make the small wavelet coefficients and some noise removed. The high-frequency parts in spherical coordinate domain are coded by improved SPIHT algorithm. Finally, based on the multi-scale edge of medical image, it can be fused and reconstructed. Experimental results indicate the novel approach is effective and very useful for transmission of big-data medical image(especially, in the wireless environment).

• Journal of Electrical Engineering and Technology
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• v.13 no.6
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• pp.2364-2375
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• 2018

Two-phase simultaneous excitation mode of the switched reluctance motor (SRM) has been shown to effectively improve the average torque output compared with traditional single-phase excitation mode. But the torque ripple of the two-phase excitation SRM with traditional winding distribution increases because of the inconsistent electromagnetic field. To reduce the torque ripple, a two-phase excitation 8/6 SRM with novel winding distribution is proposed in this paper. The static torques generated by various magnetic circuits are analyzed and obtained to verify the torque increase. Then the electromagnetic characteristics of the proposed SRM are investigated by the numerical calculation method in detail, including flux linkage, inductance, and torque. Finally, an experiment for measuring the SRM static electromagnetic characteristics and dynamic performance is designed and performed based on the novel mode, and the comparing results show that the proposed two-phase SRM is effective.

• Journal of Electrical Engineering and Technology
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• v.13 no.6
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• pp.2158-2167
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• 2018

This paper analyzed the magnetic field produced by the cylindrical stator coils of permanent magnet spherical motor (PMSM). The elliptic equations about the vector magnetic potential were given. Given that the eddy current effects are neglected, the magnet field of the PMSM is regarded as irrotational field, which can be calculated by scalar magnetic potential. The current density of cylindrical stator coil was proposed based on the definition of current density. The expression of current density of stator coil was obtained according to the double Fourier series decomposition and spherical harmonic functions. Then the magnetic flux density for scalar magnetic potential was derived. Further, the influence of different parameters on radial flux density was also analyzed. Finally, the results by the analytical method in this paper were validated by finite element analysis (FEA).