• 대한본초학회지
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• 제32권2호
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• pp.77-85
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• 2017

Objectives : This study aimed to investigate the unknown mechanisms behind the anti- inflammatory activity of Hyeonto-dan(HT) 70% ethanol extract on LPS-stimulated RAW 264.7 cells. Methods : Cells were treated with Hyeonto-dan 1 h prior to addition of 200 ng/mL of LPS. Cell viability was measured by the MTS assay. Nitric oxide levels were determined by the Griess assay. $PGE_2$ were measured using EIA kit. Pro-inflammatory cytokine production was measured by the enzyme-linked immunosorbent assay (ELISA). The expression of COX-2, iNOS, and MAPKs was investigated by Western blot, qRT-PCR. $NF-{\kappa}B$/p65 localization and interaction of the TLR-4 receptor with LPS was examined by immunofluorescence assays. Results : Hyeonto-dan had no cytotoxicity at the measured concentration. Hyeonto-dan inhibited NO production and pro-inflammatory cytokines such as IL-6, $TNF-{\alpha}$, and PGE2 as well as the protein and mRNA expression of iNOS and COX-2. Moreover, Hyeonto-dan inhibited the interaction between LPS and TLR-4 in murine macrophages. It suppressed phosphorylation of extracellular signal-regulated kinase (ERK 1/2), c-jun N-terminal kinase (JNK 1/2) and p38. Finally, it inhibited translocation of $NF-{\kappa}B$ in response to competitive LPS. Conclusions : Based on the results of this study, Hyeonto-dan inhibited the binding of TLR-4 receptor to LPS and inhibited the phosphorylation of extracellular signaling pathway MAPKs. These inhibitory effects are thought that the amount of $NF-{\kappa}B$ delivered to the nucleus was decreased and the inflammatory reaction was prevented by decreasing the production of LPS-induced $PGE_2$, NO, IL-6 and $TNF-{\alpha}$.

• Journal of Veterinary Science
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• 제21권5호
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• pp.80.1-80.13
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• 2020

Background: In suckling piglets, transmissible gastroenteritis virus (TGEV) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. This study explored methods of preventing or inhibiting their production. Bovine antimicrobial peptide-13 (APB-13) has antibacterial, antiviral, and immune functions. Objectives: This study analyzed the efficacy of APB-13 against TGEV through in vivo and in vitro experiments. Methods: The effects of APB-13 toxicity and virus inhibition rate on swine testicular (ST) cells were detected using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT). The impact of APB-13 on virus replication was examined through the 50% tissue culture infective dose (TCID₅₀). The mRNA and protein levels were investigated by real-time quantitative polymerase chain reaction and western blot (WB). Tissue sections were used to detect intestinal morphological development. Results: The safe and effective concentration range of APB-13 on ST cells ranged from 0 to 62.5 ㎍/mL, and the highest viral inhibitory rate of APB-13 was 74.1%. The log₁₀TCID₅₀ of 62.5 ㎍/mL APB-13 was 3.63 lower than that of the virus control. The mRNA and protein expression at 62.5 ㎍/mL APB-13 was significantly lower than that of the virus control at 24 hpi. Piglets in the APB-13 group showed significantly lower viral shedding than that in the virus control group, and the pathological tissue sections of the jejunum morphology revealed significant differences between the groups. Conclusions: APB-13 exhibited good antiviral effects on TGEV in vivo and in vitro.

• Advances in nano research
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• 제12권6호
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• pp.639-652
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• 2022

The prolyl 3-hydroxylase family member 4 (P3H4), is associated with post-translational modification of fibrillar collagens and aberrantly activated in cancer leading to tumor progression. However, its role in clear cell renal cell carcinoma (ccRCC) is still unknown. Here we reported that P3H4 was highly expressed in renal cancer tissues and significantly positive correlated with poor prognosis. Knockdown of P3H4 inhibited the proliferation, migration and metastasis of renal cancer cells in vitro and in vivo, and also, overexpression of it enhanced the oncogenic process. Mechanistically, P3H4 depletion decreased the levels of GDF15-MMP9 axis and repressed its downstream signaling. Further functional studies revealed that inhibition of GDF15 suppressed renal cancer cell growth and GDF15 recombinant human protein (rhGDF15) supplementation effectively rescued the inhibitory effect induced by P3H4 knockdown. Moreover, decreased levels of MMP9 caused by inhibition of P3H4-GDF15 signaling constrained the expression of PD-L1 and suppression of P3H4 accordingly promoted anti-tumor immunity via stimulating the infiltration of CD4+ and CD8+ T cells in syngeneic mice model. Taken together, our findings firstly demonstrated that P3H4 promotes ccRCC progression by activating GDF15-MMP9-PD-L1 axis and targeting P3H4-GDF15-MMP9 signaling pathway can be a novel strategy of controlling ccRCC malignancy.

• 문화기술의 융합
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• 제7권4호
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• pp.337-342
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• 2021

본 연구의 목적은 아이코닉스를 중심으로 문화 콘텐츠 기업의 환경 및 전략 행태를 분석하고 지속적인 경쟁우위를 창출하기 위해 아이코닉스가 나아가야 할 전략적 제언을 도출하고자 한다. 분석 결과 아이코닉스는 이미 세계시장을 주도하는 미국, 유럽의 메이저들과 당당하게 경쟁할 수 있는 전천후 엔터테인먼트 콘텐츠 사업자로 발전해나가고자 하는 그들의 미션에 걸맞게 콘텐츠의 기획에서부터 사업에 이르는 수직 계열화된 개발-비즈니스 시스템 구축하고 있으며, 국내 및 해외를 포괄하는 강력한 글로벌 비즈니스 네트워크를 단계적으로 구축해나가며 고도의 글로벌 전략을 취하고 있다. 하지만 뽀로로의 성공에 의존하거나 조직 구조 내의 다양한 문제점으로 인해 한계에 부딪히고 있다. 이에 엔터테인먼트 산업 각 부문별 선도 회사들의 참여와 사업적 연계를 통해 콘텐츠의 부가가치를 극대화할 수 있는 One Source Multi Channel/Multi Use 전략을 기반으로 본 연구에서 제시한 다양한 전략적 제언들을 실행한다면 안정화된 Total Entertainment Contents 산업에서의 선두주자로 자리매김할 것이다.

• Parasites, Hosts and Diseases
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• 제59권6호
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• pp.625-634
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• 2021

Based on the field investigations in 91 investigation sites (counties) in southwest China between 2001 and 2019, the present paper reported the chigger mites on A. agrarius mice in southwest China for the first time by using a series of statistical methods. From 715 striped field mice captured in 28 of 91 investigated sites, only 255 chiggers were collected, and they were identified as 14 species, 6 genera in 3 subfamilies under 2 families. Of 715 A. agrarius mice, only 24 of them were infested with chigger mites with low overall prevalence (P_M=3.4%), overall mean abundance (M_A=0.36 mites/host) and overall mean intensity (M_I=10.63 mites/host). The species diversity and infestation of chiggers on A. agrarius were much lower than those previously reported on some other rodents in southwest China. On a certain species of rodent, A. agrarius mouse in southwest China seems to have a very low susceptibility to chigger infestations than in other geographical regions. Of 14 chigger species, there were 3 dominant species, Leptotrombidium sialkotense, L. rupestre and Schoengastiella novoconfuciana, which were of aggregated distribution among different individuals of A. agrarius hosts. L. sialkotense, one of 6 main vectors of scrub typhus in China, was the first dominant on A. agrarius. The species similarity of chigger mites on male and female hosts was low with C_SS=0.25, and this reflects the sex-bias of different genders of A. agrarius mice in harboring different chigger species.

• 한국콘텐츠학회논문지
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• 제22권2호
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• pp.147-161
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• 2022

본 연구는 2000년 이후 시게루 반이 설계한 대표적인 임시전시관 3곳과 상설전시관 3곳을 대상으로 반의 전시공간의 생태적 특성과 디자인 특성에 대한 변화를 고찰하였다. 생태건축에 관한 개념 및 특성, 전시공간의 디자인 특성 등에 대한 문헌고찰을 통해 전시공간의 디자인 특성과 생태건축의 디자인요소에 대한 분석틀을 도출하였다. 연구 결과 건축규모, 공간구성, 기능, 재료 및 기술 측면에서 임시전시관과 상설전시관 사이에 큰 변화가 있다. 시게루 반의 임시전시관은 재활용 가능한 재료로 종이튜브, 컨테이너 등을 현장에서 전시 위주의 단층 공간으로 조립해 조립방법이 단순하고 공기가 짧아 전시회가 끝난 후 전시관이 철거되어 그 재료가 다음 전시장으로 옮겨지거나 재활용됐다. 한편 상설전시관은 철근콘크리트를 주체로 하고 목재와 유리재료를 다량으로 사용해 전시공간과 휴게공간을 분리시킨 다층 복합문화공간을 조성했다. 또한 시게루 반의 전시관을 영구건축으로서 친환경적인 재료와 혁신적인 구조기술, 기후설계 개념을 적용하는 등 지속가능한 건축을 실현하고 있다. 생태적 특성 측면에서 임시전시관은 건축자재를 모두 재활용할 수 있어 철거 후 산업폐기물이 발생하지 않았다. 상설전시관은 지붕, 벽면 등에 친환경 목재를 사용해 교체와 보수가 용이하며 두 가지 유형의 전시관은 모두 자연채광과 환기를 통해 자원과 에너지를 절약을 등 생태적 건축을 보다 지속가능한 방향으로 변화시켜 가고 있다.

• Parasites, Hosts and Diseases
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• 제61권3호
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• pp.272-281
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• 2023

Chigger mites are the vector of scrub typhus. This study estimates the infestation status and ecological characteristics of chiggers on the chestnut white-bellied rat Niviventer fulvescens in Southwest China between 2001 and 2019. Chiggers were identified under the microscope, and infestation indices were calculated. The Preston's log-normal model was used to fit the curve of species abundance distribution. A total of 6,557 chiggers were collected in 136 of 342 N. fulvescens rats, showing high overall infestation indices (prevalence=39.8%, mean abundance=19.2, mean intensity=48.2) and high species diversity (S=100, H'=3.0). Leptotrombidium cangjiangense, Neotrombicula japonica, and Ascoschoengastia sifanga were the three dominant chigger species (constituent ratio=42.9%; 2,736/6,384) and exhibited an aggregated distribution among different rat individuals. We identified 100 chigger species, with 3 of them (Leptotrombidium scutellare, Leptotrombidium wenense, and Leptotrombidium deliense) as the main vectors of scrub typhus in China and nine species as potential vectors of this disease. Disease vector occurrence on N. fulvescens may increase the risk of spreading scrub typhus from rats to humans. Chigger infestation on N. fulvescens varied significantly in different environments. The species abundance distribution showed a log-normal distribution pattern. The estimated number of chigger species on N. fulvescens was 126 species.

• Computers and Concrete
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• 제32권3호
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• pp.247-261
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• 2023

Due to the non-uniform distribution of meso-structure, the diffusion of chloride ions in concrete show the characteristics of characteristics of randomness and fuzziness, which leads to the non-uniform distribution of chloride ions and the non-uniform corrosion of steel rebar in concrete. This phenomenon is supposed as the main reason causing the uncertainty of the bearing capacity deterioration of reinforced concrete structures. In order to analyze and predict the durability of reinforced concrete structures under chloride environment, the random features of chloride ions transport in concrete were studied in this research from in situ meso-structure of concrete. Based on X-ray CT technology, the spatial distribution of coarse aggregates and pores were recognized and extracted from a cylinder concrete specimen. In considering the influence of ITZ, the in situ mesostructure of concrete specimen was reconstructed to conduct a numerical simulation on the diffusion of chloride ions in concrete, which was verified through electronic microprobe technology. Then a stochastic study was performed to investigate the distribution of chloride ions concentration in space and time. The research indicates that the influence of coarse aggregate on chloride ions diffusion is the synthetic action of tortuosity and ITZ effect. The spatial distribution of coarse aggregates and pores is the main reason leading to the non-uniform distribution of chloride ions both in spatial and time scale. The chloride ions concentration under a certain time and the time under a certain concentration both satisfy the Lognormal distribution, which are accepted by Kolmogorov-Smirnov test and Chi-square test. This research provides an efficient method for obtain mass stochastic data from limited but representative samples, which lays a solid foundation for the investigation on the service properties of reinforced concrete structures.

• Animal Bioscience
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• 제36권7호
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• pp.991-1002
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• 2023

Objective: This study aimed to elucidate the underlying gene regions responsible for productive, phenotypic or adaptive traits in different ecological types of Tibetan sheep and the discovery of important genes encoding valuable traits. Methods: We used whole-genome resequencing to explore the genetic relationships, phylogenetic tree, and population genetic structure analysis. In addition, we identified 28 representative Tibetan sheep single-nucleotide polymorphisms (SNPs) and genomic selective sweep regions with different traits in Tibetan sheep by fixation index (Fst) and the nucleotide diversity (θπ) ratio. Results: The genetic relationships analysis showed that each breed partitioned into its own clades and had close genetic relationships. We also identified many potential breed-specific selective sweep regions, including genes associated with hypoxic adaptability (MTOR, TRHDE, PDK1, PTPN9, TMTC2, SOX9, EPAS1, PDGFD, SOCS3, TGFBR3), coat color (MITF, MC1R, ERCC2, TCF25, ITCH, TYR, RALY, KIT), wool traits (COL4A2, ERC2, NOTCH2, ROCK1, FGF5, SOX9), and horn phenotypes (RXFP2). In particular, a horn-related gene, RXFP2, showed the four most significantly associated SNP loci (g. 29481646 A>G, g. 29469024 T>C, g. 29462010 C>T, g. 29461968 C>T) and haplotypes. Conclusion: This finding demonstrates the potential for genetic markers in future molecular breeding programs to improve selection for horn phenotypes. The results will facilitate the understanding of the genetic basis of production and adaptive unique traits in Chinese indigenous Tibetan sheep taxa and offer a reference for the molecular breeding of Tibetan sheep.

• ALGAE
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• 제38권2호
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• pp.141-150
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• 2023

Light quality is a common environmental factor which influences the metabolism of biochemical substances in algae and leads to the response of algal growth and development. Pyropia yezoensis is a kind of economic macroalgae that naturally grows in the intertidal zone where the light environment changes dramatically. In the present study, P. yezoensis thalli were treated under white light (control) and monochromatic lights with primary colors (blue, green, and red) for 14 days to explore their physiological response to light quality. During the first 3 days of treatment, P. yezoensis grew faster under blue light than other light qualities. In the next 11 days, it showed better adaptation to green light, with higher growth rate and photosynthetic capacity (reflected by a higher rETR_max = 61.58 and E_k = 237.78). A higher non-photochemical quenching was observed in the treatment of red light than others for 14 days. Furthermore, the response of P. yezoensis to light quality also results in the difference of photosynthetic pigment contents. The monochromatic light could reduce the synthesis of all pigments, but the reduction degree was different, which may relate to the spectral absorption characteristics of pigments. It was speculated that P. yezoensis adapted to a specific or changing light environments by regulating the synthesis of pigments to achieve the best use of light energy in photosynthesis and premium growth and metabolism.