• Journal of Acupuncture Research
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• v.22 no.2
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• pp.191-201
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• 2005

Background & Objective: Articular cartilage is a potential target for drugs designed to inhibit the activity of matrix metalloproteinases (MMPs) to stop or slow the destruction of the proteoglycan and collagen in the cartilage extracellular matrix. The purpose of this study was to investigate the effects of Aralia cordata Thunb. in inhibiting the release of glycosaminoglycan (GAG), the degradation of collagen, and MMP activity in rabbit articular cartilage explants. Methods : The cartilage-protective effects of Aralia cordata Thunb. were evaluated by using glycosaminoglycan degradation assay, collagen degradation assay, colorimetric analysis of MMP activity, measurement of lactate dehydrogenase activity and histological analysis in rabbit cartilage explants culture. Results : Interleukin-la (IL-1a) rapidly induced GAG, but collagen was much less readily released from cartilage explants. Aralia cordata Thunb. significantly inhibited GAG and collagen release in a concentration-dependent manner. Aralia cordata Thunb. dose-dependently inhibited MMP-3 and MMP-13 expression and activities from IL-1a-treated cartilage explants cultures when tested at concentrations ranging from 0.02 to 0.2 mg/ml. Aralia cordata Thunb. had no harmful effect on chondrocytes viability or cartilage morphology in cartilage explants. Histological analysis indicated that Aralia cordata Thunb. reduced the degradation of the cartilage matrix compared with that of IL -1a-treated cartilage explants.

• Herbal Formula Science
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• v.29 no.4
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• pp.181-189
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• 2021

Objectives : The calyx of Diospyros kaki Thunb. has usually been used to treat obstinate hiccup. This study investigated the protective effects of Diospyros kaki using C2C12 myoblasts of H₂O₂-induced oxidative stress. Methods : Cell viability and cytotoxicity were determined by MTT assay. The level of reactive oxygen species (ROS), malondialdehyde (MDA), and reduced glutathione (GSH) were measured by using optical properties. Results : The calyx of Diospyros kaki Thunb. extract showed no toxicity to C2C12 myoblasts until 20 ㎍/mL concentration and increased cell viability compared to the H₂O₂ treated group. The calyx of Diospyros kaki Thunb. extract inhibited the production of ROS and MDA at all concentrations. In addition, the calyx of Diospyros kaki Thunb. extract increased the concentration of GSH. Conclusion : This study provides that the calyx of Diospyros kaki Thunb. can be used as a potential material that exhibit antioxidative and protective effects on H₂O₂-induced oxidative stress in C2C12 Myoblasts.

• Food Science and Preservation
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• v.21 no.1
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• pp.34-39
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• 2014

The purpose of this study was to evaluate appropriate concentrations of Houttuynia cordata Thunb. powder, which has various functions, to dried wheat flour noodles. The viscosity of the composite flour and cooking quality of the noodle samples were measured, and sensory evaluations were conducted in this study. When measured by the amylograph, the composite flour had lower viscosity in the gelatinization points, maximum viscosity, and viscosity at a temperature of $95^{\circ}C$ than those of control. However, the gap between the maximum viscosity and viscosity after 15 minutes at a temperature of $95^{\circ}C$ was not great among all of the samples. While cooked noodles containing H. cordata Thunb. powder had lower weight and lower volume than those of control, the weight of two cooked noodle samples with 0.5% and 1.5% of H. cordata Thunb. powder was insignificantly different. Cooked noodles had higher concentrations of H. cordata Thunb. powder; therefore, the L value of cooked noodles decreased, while a and b color values of the noodles increased. Color, flavor, taste, and overall acceptability of the samples with H. cordata Thunb. powder were lower than those of wheat flour noodles via sensory evaluation. In the overall acceptability, however, samples with 0.5% and 1.5% of H. cordata Thunb. powder were insignificantly different from control. As a result, it was finally suggested that optimum level of the H. cordata Thunb. powder in the product was below 0.5%.

• Korean Journal of Plant Taxonomy
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• v.45 no.3
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• pp.221-226
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• 2015

Rubus palmatus Thunb. var. palmatus (Rosaceae) was first discovered in Anmyeon-do and Geoje-do, Korea, and the new Korean name 'Gin-yip-dan-pung-ttal-gi' was given. This taxon can be distinguished from var. coptophyllus by having 3-lobed or rarely undivided leaves. We, for the first time, report the distribution of R. palmatus var. coptophyllus (A. Gray) Kuntze from the island of Geoje-do in Korea and clarified the Korean vernacular name of 'Dan-pung-ttal-gi.' Finally, we designated the lectotype of R. palmatus using C. P. Thunberg's specimen (UPS-THUNB. 12282).

• KSBB Journal
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• v.32 no.3
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• pp.218-233
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• 2017

To identify the natural and novel cosmeceutical ingredients which have whitening function, we examined the effects of tyrosinase activity and melanin production on halophyte Limonium tetragonum (Thunb.) A. A. Bullock extracts. As a result, the extracts showed whitening effect with no cytotoxicity. Melanin contents of B16F10 melanoma cells were decreased in a dose-dependent manner at 50, 100, $200{\mu}g/mL$ treatment of the extracts. In tyrosinase activity inhibition test, L. tetragonum (Thunb.) A. A. Bullock extracts showed decreased tyrosinase activity as the concentration of ${\alpha}-MSH$ was increased. Furthermore, it was observed that the tyrosinase and MITF expression was significantly downregulated by adding L. tetragonum (Thunb.) A. A. Bullock extracts in ${\alpha}-MSH$, a melanogenesis inducing material, treated B16F10 melanoma cells. These results indicate that L. tetragonum (Thunb.) A. A. Bullock extract might be an effective whitening agent by inhibit melanin formation.

• Korean Journal of Pharmacognosy
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• v.33 no.4 s.131
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• pp.324-331
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• 2002

This study was performed to investigate the protective effect of Houttuynia cordata Thunb on hepatotoxicity in carbon tetrachloride$(CCI_4)$ intoxicated rats. The examined effects hexane, chloroform, butanol and water fractions prepared from the Houttuynia cordata Thunb methanol exlract and rats were administrated with those orally once a day for successive 6 days, fellowed by treaoent with $CCl_4$ on the sixth day. After 6 days, the activities of aminotransferase, alkalinephosphatase, ${\gamma}-glutamyl$ transpeptidase, lactate dehydrogenase and contents of triglyceride, hepatic lipid peroxide in butanol fraction pretreated rats were significantly decreased compared to the only $CCl_4$ treated rats, also depletion glutathione content induced by treatment with $CCl_4$ was prevented by butanol fraction pretreated rats. In addition, activities of hepatic superoxide dismutase, catalase, glutathione peroxidase in butanol fiaction pretreated rats were significantly decreased compared to the only $CCl_4$ treated rats, but the activity of hepatic glutathione-S-transferase was not significantly effect. These results suggest that butanol fiuction of Houttuynia cordata Thunb methanol extract have potent hepatoprotective effect against carbon tetrachloride intoxicated rats.

• Journal of Species Research
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• v.9 no.2
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• pp.117-121
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• 2020

Prunus glandulosa Thunb. [=Cerasus glandulosa (Thunb.) Sokolov] (Rosaceae) is native to the warmtemperate region of China (Anhui, Fujian, Guangdong, Guangxi, Guizhou, Hebei, Henan, Jiangsu, Shaanxi, Shandong, Sichuan, Yunnan, and Zhejiang Provinces). It is naturalized in the United States (i.e. Alabama, Michigan and North Carolina) and Canada (Ontario). This species, previously only recorded as introduced or cultivated plants on the Korean Peninsula, was confirmed to be naturally distributed in the southwestern coast islands(i.e. Geomun-do, Chuja-do and an uninhabited island in Shinan-gun of Jeollanam-do). Prunus glandulosa Thunb. is distinguished from a related taxon P. japonica var. nakaii (H. Lév.) Rehder by pedicel 8-12 (-16) mm long, linear stipules, glabrous style, and pink petals. The morphological characters and illustration of P. glandulosa Thunb. are provided with line drawings and photographs from the natural habitat. In addition, it is likely that a new habitat will be found by plant biodiversity investigations through the southwestern coast islands. Further research is needed to determine its population size, distribution, and threats, as well as identify appropriate locations for conservation collection of germplasm.

• Journal of Environmental Science International
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• v.12 no.6
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• pp.599-603
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• 2003

This study investigated the effects of Houttuynia Cordata thunb(HCT) administration on the biochemical parameters of function in liver of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) treated rats. Houttuynia Cordata thunb (200mg/kg) was administeres into rats intraperitoneally for four weeks , seven days after the injection of TCDD(1$\mu\textrm{g}$/kg). We examined the antioxidative enzymatic activity by measuring the level of AST, ALT , SOD and Catalase in serum and liver tissue of rats. HCT group showed 49% of inhibitive effect in AST activity compared to TANO group. ALT level of HCT group was decreased to the level of NO group. SOD and Catalase in TANO group were lower than in NO group, but SOD and Catalase in HCT group were increased by 46％ and by 50％ respectively compared to TANO group.

• The Korean Journal of Ecology
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• v.6 no.3
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• pp.208-226
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• 1983

The vegetation of Odongdo island was surveyed over twenty-nine times from April, 1982 to August, 1983. This island is located at 127。46'05'∼127。39'37'E. longitude and 34。40'20'∼34。48'14'N. latitude. The vegetation of this island consisted of 94 families, 261 genera, 314 species, 53 varieties and 6 formae. And among them were 170 species of woody plants and 203 species of herbs. The community of evergreen broad-leaved trees comprised a total of 44 species including Sasa coreana Nakai and camellia japonica L. and the distribution of evergreen broad-leaved trees was good in this island as a whole. Sasa coreana Nakai, Castanopsis cuspidata var. sieboldii Nakai, Ficus erecta Thunb., Ficus nipponica Fr. et Sav, Stauntonia hexaphylla (Thunb.) Decne, Cinnamomum japonicum Sieb., Machilus thunbergii S. et Z., Lozoste lancifolia (S. et Z.) Bl., Ilex intergra Thunb., Camellia japonica Thunb., Hedera rhombea Bean and Ardisia japonica Bl. etc. in this island were autochthonous flora and their preservation is required.

• Journal of Applied Biological Chemistry
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• v.44 no.2
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• pp.71-76
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• 2001

Methanol extract and solvent fractions of Aster scaber Thunb. were tested to elucidate antioxidative characteristics in vitro. Methanol extract showed strong inhibition on linoleic acid autoxidation and conjugated diene production. Hexane and diethyl ether fractions showed stronger chelating effect on $10^{-4}M\;FeCl_3$ than the other fractions. Butanol fraction had the strongest capacity on $10^{-3}M\;CuSO_4$ similar to ${\alpha}$-tocopherol. Synergistic effect of butanol fraction with histidine, methionine, and lysine on linoleic acid autoxidation had been observed. Contents of total phenol, vitamin E, vitamin C, and carotenoid were evaluated. From the results, Aster scaber Thunb. could be expected to play a role as an antioxidant in vivo.