• The Research Journal of the Costume Culture
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• v.12 no.6 s.53
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• pp.1021-1030
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• 2004

The purpose of this study was to develop and make the head form by setting the region of the head for detailed measurement of the head to make the type of closely fit hat and typifying the head form of Korean women's with each region of the head based on the measurements of each region of the head according characteristics. A total of 285 Korean women's in the age group of 18 to 35 years old. It were measured through the direct measurement method by selecting 67 measurement items. As a result of the findings obtained from cluster analysis could be classified into 3 type. Type 1($46\%$) is U-shaped form, that is high in head height on sides and circumference is smallest, whose facial region and occiput is flattest of three types. Type 2($22.4\%$) is inverted triangle form, that is flat in head ridge, low in head height on sides and wide in the frontal width. Type 3($31.6\%$) is oval form, that is largest in size, has the projected occiput and is of three-dimensional form.

• The Research Journal of the Costume Culture
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• v.11 no.2
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• pp.219-230
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• 2003

The purpose of this study was to analyze the structure of consumption emotions that consumers experienced in the process of consuming apparel products. Data was collected from 144 female college students living in Busan, and analyzed by salience, diversity, H-index, Clamor's V, and multi-dimensional scaling. The results showed as following; 1. The consumption emotions related to apparel products appeared three dimensions; ‘Relaxed-tense’ dimension, ‘Pleasant-unpleasant’ dimension, and ‘Outward-inward’ dimension. Considering elements of consumption system, the dimensions of consumption emotions in relation to apparel performances were 'Pleasant-unpleasant' and ‘Outward-inward’. The dimensions of consumption emotions experienced in usage situations were ‘Relaxed-tense’ and ‘pleasant-unpleasant’. The consumption emotions related to specific products were composed of ‘Pleasant-unpleasant’ dimension and ‘Outward-inward’ dimension. 2. As the multi-dimension map of this study has much space, it suggested that the scope of consumption emotions related to apparel products was more limited than those related to general situations and products. 3. The structure of consumption emotions in relation to apparel performances appeared to be bisected, while those related to usage situations showed relatively to be dispersed. 4. Although Pleasant-unpleasant dimension was consistent with results of prestudies, the dimensions of ‘Relaxed-tense’ and ‘Outward-inward’ were newly confirmed as the dimensions of consumption emotions related to apparel products. Therefore, consumer's consumption emotions of apparel products were composed of three dimensions, tended to be more limited than those of general consumption situations and products, and differentiated across apparel performances, usage situation, and specific products.

• Archives of Pharmacal Research
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• v.21 no.4
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• pp.398-405
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• 1998

We investigated the effects of triterpene acids (TAs), ursolic acid (UA) and oleanolic acid (OA), on the induction of proliferation and differentiation of normal rat mammary epithelial cells (RMEC) or organoids cultured in Matrigel or primary culture system. To elucidate the effects, we tested their differentiation inducing activities with intercellular communication ability, cell cycle patterns, induction of apoptosis, and morphological differentiation in the three dimensional extracellular culture system. To study the changes of RMEC subpopulation in culture, the cultured cells were isolated, immunostained with peanut lectin (PNA) and anti-Thy-1.1 antibody and then analyzed with flow cytometry. Four different subpopulations, such as PNA and Thy-1.1 negative cells (B-), PNA positive cells (PNA+), Thy-1.1 positive cells (Thy-1.1+), PNA and Thy-1.1 positive cells (B+), were obtained and the size of each subpopulation was changed in culture with time in the presence of TAs. Intercellular communication was observed in culture for 7 days in TAs-treated cells, but not in culture for 4 days with scrape-loading dye transfer technique. $G_2$/M phase cells and the number of apoptotic population were increased in TAs-treated groups in cell cycle analyses. S phase fractions were reduced and the change of $G_1$ phase cells was not observed. The colonies with distinct multicelfular structures, such as stellate, ductal, webbed, squamous, lobulo-ductal colonies, were observed in Matrigel culture and the frequencies of each colony were changed in the presence of TAs. These results suggest that UA and OA have differentiation inducing effects on rat mammary epithelial cells in primary or in Matrigel culture.

• Proceedings of the Korean Institute of Interior Design Conference
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• 2007.11a
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• pp.37-43
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• 2007

In recent years, the cultural industry is considered to a major industry in the 21 century, and performing culture is growing rapidly in Korea. Also, rapid development in terms of quality and quantity can be found in the diversification of usage, a trend focusing on the space for performance, and an emphasis on the accessibility between the stage and auditorium. In addition, active research is being conducted on the performing arts facilities, a relationship between the stage and auditorium, and a space programfor each space considering users. However, most of the current studies are conducted byviewing the facilities in a two-dimensional space. Similar to other cultural facilities, such as museums and galleries, performing arts facilities have been planned in the thinking that the ratio of capacity increases in proportion to the area. Considering a dimensional capacity in the spatial composition of performing arts facilities is important, but it has been ignored until now. Therefore, in order to analyze performing arts facilities in the three dimensional approach, this study is conducted centering on the actual capacity and provides basic data that can be used for planning a construction of future performing arts facilities.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.10
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• pp.551-558
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• 2017

To study and validate tissue-specific promoters and vectors, it is important to develop cell culture systems that retain the tissue and species specificity. Such systems are attractive alternatives to transgenic animal models. This study established a line of porcine mammary gland epithelial cells (PMECs) from a primary culture based on the cellular morphology and mRNA levels of porcine beta-casein (CSN2). The selected PMECs were stained with the cytokeratin antibody, and were shown to express milk protein genes (CSN2, lactoferrin, and whey acidic protein). In addition, to confirm the acini structure of PMEC932-7 in 3D culture, live cells were stained with SYTO-13 dye, which binds to nucleic acid. The acini of these PMECs on matrigel were formed by the aggregation of peripheral cells and featured a hollow lumens. The system was demonstrated by testing the effects of the culture conditions to cell culture including cell density and matrigel methods of the PMECs. These results suggest that PMECs possess the genetic and structural features of mammary epithelial cells.

• Journal of Embryo Transfer
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• v.32 no.4
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• pp.343-351
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• 2017

Porcine spermatogonial stem cells (SSCs) prefer three-dimensional (3D) culture systems to 2D ones for the maintenance of self-renewal. Of the many 3D culture systems, agar-based hydrogels are candidates for supporting porcine SSC self-renewal, and there are various types of agar powder that can be used. In this study, we sought to identify an agar-based 3D hydrogel system that exhibited strong efficacy in the maintenance of porcine SSC self-renewal. First, 3D hydrogels with different mechanics were prepared with various concentrations of Bacto agar, lysogeny broth (LB) agar, and agarose powder, and the 3D hydrogel with the strongest alkaline phosphatase (AP) activity and greatest increase in colony size was identified for the different types of agar powder. Second, among the porcine SSCs cultured in the different 3D hydrogels, we analyzed the colony formation, morphology, and size; AP activity; and transcription and translation of porcine SSC-related genes, and these were compared to determine the optimal 3D hydrogel system for the maintenance of porcine SSC self-renewal. We found that 0.6% (w/v) Bacto agar-, 1% (w/v) LB agar-, and 0.2% (w/v) agarose-based 3D hydrogels showed the strongest maintenance of AP activity and the most pronounced increase in colony size in the culture of porcine SSCs. Moreover, among these hydrogels, the strongest transcription and translation of porcine SSC-related genes and largest colony size were detected in porcine SSCs cultured in the 0.2% (w/v) agarose-based 3D hydrogel, whereas there were no significant differences in colony formation and morphology. These results demonstrate that the 0.2% (w/v) agarose-based 3D hydrogel can be effectively used for the maintenance of porcine SSC self-renewal.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.3
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• pp.369-385
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• 1996

The eco-hydrodynamic model was used to estimate the primary productivity of the oyster culture grounds in Kamak Bay. It is composed of the three-dimensional hydrodynamic model for the simulation of water flow and ecosystem model for the simulation of phytoplankton. The ecosystem model was applied to simulate phytoplankton biomass during culturing period in condition of no oyster culture grounds. The field surveys were conducted from May, 1994 to March, 1995 in Kamak bay. The results showed the concentration of chlorophyll $\alpha$ to be $1.00\~23.28\;{\mu}g/l$ in the surface layer, $1.27\~29.97\;{\mu}g/l$ in the middle layer and $1.23\~23.08\;{\mu}g/l$ the bottom layer. In monthly variations of chlorophyll $\alpha$ concentration, very high concentration were found in July, 1994 and very low concentrations in December, 1994. As the results of three-dimensional hydrodynamic simulation, the computed tidal currents ave mainly toward the inner part of bay through Yeosu Harbor and the southern mouth of a bay during the flood tide. The computed residual currents were dominated southward in Yeosu Harbor and eastward in the mouth of bay and also showed strong clockwise water circulation at the mouth of bay. The pattern between the simulated and observed tidal ellipses at three stations was very similar. The mean relative errors of all levels between the simulated and observed phytoplankton biomass at 14 stations in Kamak Bay were $13.81\%,\;9.31\%\;and\;17.84\%$, respectively. The results of phytoplankton biomass simulation showed that the biomass increased from June to September and rapidly decreased to December and then slowly increased to March. Primary productivity was estimated in the range of $0.99\~10.20gC/m^2/d$ with the average value of $4.43gC/m^2/d$ in condition of no oyster culture grounds. Primary productivity was rapidly increased from lune to August and rapidly decreased to December and then slowly increased from January to March in Kamak Bay.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.141-144
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• 2000

Highly open porous polymer matrices are required for high density cell seeding, efficient nutrient, and oxygen supply to the cells cultured in the three dimensional matrices. However, there are severe problems of mass transfer limitations within the cell/scaffolds culture system. Thus we hypothesize that continuos-flow culture conditioning of cells with the scaffolds may improve the cell viability and the differentiated function. In this study, we fabricated porous PLGA scaffolds by using gas-foaming/salt-leaching method as previous described. Viscous PLGA gel paste contains ammonium bicarbonate particulates, acting as a gas-foaming agent as well as a salt-leaching porogen, were cast into Teflon mold and dried. Ammonium bicarbonate salt upon contact to an acidic aqueous solution evloves gaseous ammonia and carbon dioxide by itself. And we conjugated galactose moiety [AGA; $N-(aminobuty1)-O-{\beta}-D-galactopyranosyl-(1{\rightarrow}4)-D-glucoamide]$ to the terminal end group of a PLGA to increase the cell adhesion and matain the differentiated function of hepatocytes. Cell-seeded scaffolds were secured in a flow bioreactor chamber and exposed to continuous flow at 5 ml/min. As a result of our study, the high yield of hepatocytes attachment was accomplished by increasing the concentration of PLGA-AGA conjugate in polymer scaffolds and cells in the scaffolds under continuos flow condition maintained a high level of viability and albumin secretion rate of cultured hepatocytes showed a higher level that of control groups.

• Biotechnology and Bioprocess Engineering:BBE
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• v.6 no.4
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• pp.264-268
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• 2001

Aggregates of specific cells are often regarded as better from in artificial organs and mammalian cell bioreactors in terms of cell-specific functionality. In this study, the morphology and liver specific functions of freshly harvested primary rat hepatocytes, which were cultivated as spheroids and entrapped in a synthetic thermo-reversible extracellular matrix, were examined and compared to a control (hepatocytes in single cell form). A copolymer of N-isopropylacrylamide(98 mole % in feed) and acrylic acid (poly (NiPAAm-co-AAC)), a thermo- reversible copolymer gel ma- trix, was used to entrap hepatocytes either in spheroids or single cells. During a 7-day culture pe-riod, the spheroids maintained higher viability and produced albumin and urea at a relatively con-stant rate, while, the single cell culture showed a slight increase in cell numbers and a reduction in albumin secretion Hepatocytes cultrured as spheroids present a potentially useful three-dimensional cell culture system for application in bioartificial liver device.

• The Research Journal of the Costume Culture
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• v.16 no.4
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• pp.661-680
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• 2008

The purpose of this study is to examine to change in the Chinese women's clothing according to the inflow of Western culture in the first half of the both century As for the research method, both literature and visual data are reviewed. Research results are as follows: The Step of transition in clothing by period followed beginning stage, adopted stage, early developed stage, fully developed stage, and static stage. fully developed stage showed a perfect compromise while being applied to Chinese costume with being introduced a three-dimensional cutting method of western clothing even to the constitution as well as to the appearance. Design elements from western clothing are as follows. The transition of silhouette is box silhouette, trapeze silhouette, shift silhouette, and fitted silhouette. In detail and trimming, tailored, band, convertible, shirts collar and zigzag, scallop, camisole neckline and puff, cap sleeve and flounced, bell shaped cuffs and western button, zipper, etc. are adopted. Finally, the Chinese modern style are formed by accepting and transforming the western clothing based on the traditional Chinese clothing.