• Title/Summary/Keyword: Three-dimensional culture

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The Comparison of Foot Shape Classification Methods (발 형태 분류 방법 비교 연구)

  • Choi, Sun-Hui;Chun, Jong-Suk
    • The Research Journal of the Costume Culture
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    • v.15 no.2 s.67
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    • pp.252-264
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    • 2007
  • The purpose of this study was to compare two analytical methods classifying foot shape. The methods compared were cluster analysis method and foot index analysis method. This study defined the women's foot shape by these methods. 39 foot measurements which were automatically collected using the three dimensional foot scanner were analyzed. 203 Korean women in age 20s were participated in the anthropometric survey. Their foot shapes were classified into 5 foot types by cluster analysis: short & slim shape, flat shape, short & slender shape with slightly distorted toe, long and big shape, and short & wide shape. The foot measurements were also analyzed by the ratio of foot width and length. Five foot types that were classified by cluster analysis and three foot types that were classified by the foot index were compared. The comparison shows that cluster analysis precisely defined foot shapes. It was suggested that made-to-measure shoes making industry may adopt the foot shape analysis method utilizing cluster analysis.

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Establishment of Immortalized Primary Human Foreskin Keratinocytes and Their Application to Toxicity Assessment and Three Dimensional Skin Culture Construction

  • Choi, Moonju;Park, Minkyung;Lee, Suhyon;Lee, Jeong Woo;Cho, Min Chul;Noh, Minsoo;Lee, Choongho
    • Biomolecules & Therapeutics
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    • v.25 no.3
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    • pp.296-307
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    • 2017
  • In spite of frequent usage of primary human foreskin keratinocytes (HFKs) in the study of skin biology, senescence-induced block-age of in vitro proliferation has been a big hurdle for their effective utilization. In order to overcome this passage limitation, we first isolated ten HFK lines from circumcision patients and successfully immortalized four of them via a retroviral transduction of high-risk human papillomavirus (HPV) E6 and E7 oncogenes. We confirmed expression of a keratinocyte marker protein, keratin 14 and two viral oncoproteins in these immortalized HFKs. We also observed their robust responsiveness to various exogenous stimuli, which was evidenced by increased mRNA expression of epithelial differentiation markers and pro-inflammatory genes in response to three reactive chemicals. In addition, their applicability to cytotoxicity assessment turned out to be comparable to that of HaCaT cells. Finally, we confirmed their differentiation capacity by construction of well-stratified three dimensional skin cultures. These newly established immortalized HFKs will be valuable tools not only for generation of in vitro skin disease models but also for prediction of potential toxicities of various cosmetic chemicals.

A Study on Clothing Design applying Quilt - focused on a butterfly motif - (누비를 응용한 의상디자인 연구 -나비 모티브를 중심으로 -)

  • 신혜원;김정혜
    • Journal of the Korean Society of Costume
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    • v.50 no.7
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    • pp.75-96
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    • 2000
  • Modern clothing is approached in the level of art, and it becomes the means of expressing individuality in modern society uniformed by the development of information society. In this modern society, modern men need the recovery of humanity and expectations of handcrafted skills for the succession of tradition culture. Prior to expressing individuality, we should examine our traditional culture and combine it with western culture. Quit started to be used for practical purpose such as life items, but it is expanded to the fields of art. Used in dress and its ornaments design, quilt is often applied to the addition of aesthetic factors or cubic material feelings by transforming its warming effect. Hereby, this study has a purpose to create high value added modern dross and its ornaments design by expressing the modern clothing is approached in the level of art, and it becomes the means of expressing individuality roe-dimensional characteristics of quilt, and applying the color combination and the surface of butterfly wings to dress and its ornaments design. The following are the results of this study. 1. The concept of quilt started for practical purposes, but it is expanded to decoration in modern times, and it is confirmed that quilt can be variously applied to handcrafted modern design. 2. Quilt removes the plane character of textile and it riches the three-dimensional material of dress and its ornaments. Applying these characteristics, the expression of transparent wings were possible with 3 transparent layers of textile. 3. The spledid color of butterfly wings are expressed by coloring oganza and felt, and the various colors of felt showed rich color gradation. 4. The form and pattern of butterfly wings are applied as modeling form and line, and the transformation. repetition and expansion of unit forms determined the form of quilting lines. By designing the characteristics of back wings for the composition line of clothing, the form characteristics of a motif could be emphasized. 5. By using felt, oganza, Damdam yarn and ostritch feathers in expressing butterfly wings, the warm material of Linbun is felt, and the tip hair of wings are expressed by croche techniques using Damdam yarn.

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OSTEOGENIC ACTIVITY OF CULTURED HUMAN PERIOSTEAL-DERIVED CELLS IN A THREE DIMENSIONAL POLYDIOXANONE/PLURONIC F127 SCAFFOLD (Polydioxanone/pluronic F127 담체에 유입된 골막기원세포의 조골활성)

  • Lee, Jin-Ho;Oh, Se-Heang;Park, Bong-Wook;Hah, Young-Sool;Kim, Deok-Ryong;Kim, Uk-Kyu;Kim, Jong-Ryoul;Byun, June-Ho
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.31 no.6
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    • pp.478-484
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    • 2009
  • Three-dimensional porous scaffolds play an important role in tissue engineering strategies. They provide a void volume in which vascularization, new tissue formation, and remodeling can occur. Like any grafted materials, the ideal scaffold for bone tissue engineering should be biocompatible without causing an inflammatory response. It should also possess biodegradability, which provides a suitable three-dimensional environment for the cell function together with the capacity for gradual resorption and replacement by host bone tissue. Various scaffolds have already been developed for bone tissue engineering applications, including naturally derived materials, bioceramics, and synthetic polymers. The advantages of biodegradable synthetic polymers include the ability to tailor specific functions. The purpose of this study was to examine the osteogenic activity of periosteal-derived cells in a polydioxanone/pluronic F127 scaffold. Periosteal-derived cells were successfully differentiated into osteoblasts in the polydioxanone/pluronic F127 scaffold. ALP activity showed its peak level at 2 weeks of culture, followed by decreased activity during the culture period. Similar to biochemical data, the level of ALP mRNA in the periosteal-derived cells was also largely elevated at 2 weeks of culture. The level of osteocalcin mRNA was gradually increased during entire culture period. Calcium content was detactable at 1 week and increased in a time-dependent manner up to the entire duration of culture. Our results suggest that polydioxanone/pluronic F127 could be a suitable scaffold of periosteal-derived cells for bone tissue engineering.

Lower-body figure analysis of Chinese adult women

  • Cha, Sujoung
    • The Research Journal of the Costume Culture
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    • v.22 no.6
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    • pp.965-978
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    • 2014
  • To determine the parameters to be considered when designing lower-body apparel, we analyzed the lower-body figures of adult women in their early 20s from Shanghai, China, using 3-dimensional whole body scanner. Thirty-nine lower-body-related measurements were used to analyze the figures of 210 Chinese women. Obesity and height of the lower body, length from waist to crotch, shape of abdomen, and leg bone length were analyzed. Factor analysis was performed and the results were classified into three clusters. The first cluster describes the obesity of the lower body, the second denotes small hip measurement, and the third describes slim and long legs. This is the first study to quantify figure analysis of the lower body of Chinese women using 3-dimensional body measurements. The findings of this study will provide concrete information regarding crotch width, crotch length, inseam, outseam, waist slope, etc., for designing trouser patterns for Chinese adult women.

Study of Fashion Design Applying the Formative Beauty of Architectural Works by Antoni Gaudi (패턴 절개를 응용한 의상의 조형적 형태미의 표현 연구 - 안토니오 가우디 건축 작품 형태를 중심으로 -)

  • Shin, Hyo-Jung;Lee, Young-Min
    • The Research Journal of the Costume Culture
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    • v.17 no.5
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    • pp.849-865
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    • 2009
  • This research is a study of fashion design that applied formative features of formal beauty of architecture into clothing design; we focused on Gaudi's architectural style as well as Art Nouveau style that became popular from the end of the 19th century to the beginning of the 20th century. We noted that in general the simple and flat features of cloth impose a limitation on the expression of formal features in clothing design, but a unique diversity of designs can be achieved, evoking a sense of freshness by an ideal combination of flat patterns and draping. The aim of this research is to present a possibility of extending the sphere of design expression by creating three-dimensional clothes with pattern-cutting skills and applications of three-dimensional patterns as well as flat patterns found in Gaudi's works of architecture that are distinguished in curvaceousness and formal beauty. As for the research method, we reviewed previous studies by making a close review of books, papers, the pictures and web sites related to this topic. We made our clothes on the basis of this theoretical consideration. We found the following points. First, by presenting a work of fashion inspired by architectural designs, we realized that formal beauty in architect can become a motive for clothing design in a broad scale by noting the formal images, decoration details, and formative features of architectural works. Second, the characteristic lines of Gaudi's architecture are suitable to be adapted for expressing the detailed lines of decoration in clothes. Third, we can express formative beauty in clothes by highlighting the variation of shapes and lines through various attempts of change in background pattern, even though there is a limitation in the availability of cloth material because we must choose pieces of cloth with right texture and thickness that can be cut and sewn appropriately to express formative beauty. Fourth, we confirmed that it was possible to create unique formative designs by a creative application of both flat and three-dimensional cutting.

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Three-Dimensional Skin Tissue Printing with Human Skin Cell Lines and Mouse Skin-Derived Epidermal and Dermal Cells

  • Jin, Soojung;Oh, You Na;Son, Yu Ri;Kwon, Boguen;Park, Jung-ha;Gang, Min jeong;Kim, Byung Woo;Kwon, Hyun Ju
    • Journal of Microbiology and Biotechnology
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    • v.32 no.2
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    • pp.238-247
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    • 2022
  • Since the skin covers most surfaces of the body, it is susceptible to damage, which can be fatal depending on the degree of injury to the skin because it defends against external attack and protects internal structures. Various types of artificial skin are being studied for transplantation to repair damaged skin, and recently, the production of replaceable skin using three-dimensional (3D) bioprinting technology has also been investigated. In this study, skin tissue was produced using a 3D bioprinter with human skin cell lines and cells extracted from mouse skin, and the printing conditions were optimized. Gelatin was used as a bioink, and fibrinogen and alginate were used for tissue hardening after printing. Printed skin tissue maintained a survival rate of 90% or more when cultured for 14 days. Culture conditions were established using 8 mM calcium chloride treatment and the skin tissue was exposed to air to optimize epidermal cell differentiation. The skin tissue was cultured for 14 days after differentiation induction by this optimized culture method, and immunofluorescent staining was performed using epidermal cell differentiation markers to investigate whether the epidermal cells had differentiated. After differentiation, loricrin, which is normally found in terminally differentiated epidermal cells, was observed in the cells at the tip of the epidermal layer, and cytokeratin 14 was expressed in the lower cells of the epidermis layer. Collectively, this study may provide optimized conditions for bioprinting and keratinization for three-dimensional skin production.