• Title/Summary/Keyword: Three-dimensional culture

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[2-(diphenyl hydroxy-methyl)pyrrolidine]-AlH 유도체와 방향족 케톤의 선택적 환원에 대한 이론적 연구 (Theoretical Study on the Selective Reduction of Chiral [2-(diphenyl hydroxy-methyl)pyrrolidine]-AlH Derivatives and Aromatic Ketone)

  • 이철재;김종미
    • 문화기술의 융합
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    • 제7권2호
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    • pp.389-394
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    • 2021
  • 본 연구에서는 알콕시-아민-알루미늄 유도체인 DPHMP-AlH와 프로피오페논, 부티로페논과의 분자구조 및 경계궤도함수의 특성을 알아보았다. 또한, 전이상태의 입체구조 및 열역학적 파라미터를 계산하여 최종 생성물인 (R),(S)-페닐프로판올과 (R),(S)-페닐부탄올의 선택적 환원에 미치는 영향을 조사하였다. 그 결과 입체 분자구조적 안정성을 고려해 볼 때 (S)형 DPHMP-AlH와 알킬페논의 전이상태가 더 안정한 것으로 나타났으며, 이 결과로부터 DPHMP-AlH와 알킬페논의 선택적 환원으로 얻어진 최종 결과물은 (S)-(1)-페닐프로판올과 (S)-(1)-페닐부탄올의 형성이 유리한 것을 확인하였다.

Effect of endometrial cell-conditioned medium and platelet-rich plasma on the developmental competence of mouse preantral follicles: An in vitro study

  • Taghizabet, Neda;Bahmanpour, Soghra;Zarei-fard, Nehleh;Mohseni, Gholamreza;Aliakbari, Fereshteh;Dehghani, Farzaneh
    • Clinical and Experimental Reproductive Medicine
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    • 제49권3호
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    • pp.175-184
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    • 2022
  • Objective: The aim of this study was to evaluate the impacts of platelet-rich plasma (PRP) and conditioned medium (CM) derived from endometrial stromal cells on mouse preantral follicle culture in a two-dimensional system to produce competent mature oocytes for fertilization. Methods: In total, 240 preantral follicles were isolated from female mouse ovarian tissue and divided into four groups. The preantral follicles were isolated three times for each group and then cultured, respectively, in the presence of alpha minimum essential medium (control), PRP, CM, and PRP+CM. The in vitro growth, in vitro maturation, and cleavage percentage of the preantral follicles were investigated. Immunocytochemistry (IHC) was also conducted to monitor the meiotic progression of the oocytes. Additionally, the mRNA expression levels of the two folliculogenesis-related genes (Gdf9 and Bmp15) and two apoptosis-related genes (Bcl2 and Bax) were investigated using real-time polymerase chain reaction. Results: In the PRP, CM, and PRP+CM groups, the preantral follicle maturation (evaluated by identifying polar bodies) were greater than the control group. The cleavage rate in the CM, and PRP+CM groups were also greater than the control group. IHC analysis demonstrated that in each treatment group, meiotic spindle was normal. In the PRP+CM group, the gene expression levels of Bmp15, Gdf9, and Bcl2 were greater than in the other groups. The Bax gene was more strongly expressed in the PRP and control groups than in the other groups. Conclusion: Overall, the present study suggests that the combination of CM and PRP can effectively increase the growth and cleavage rate of mouse preantral follicles in vitro.

Effect of Wnt signaling pathway activation on the efficient generation of bovine intestinal organoids

  • Park, Kang Won;Yang, Hyeon;Wi, Hayeon;Ock, Sun A;Lee, Poongyeon;Hwang, In-Sul;Lee, Bo Ram
    • 한국동물생명공학회지
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    • 제37권2호
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    • pp.136-143
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    • 2022
  • Recent progress has been made to establish intestinal organoids for an in vitro model as a potential alternative to an in vivo system in animals. We previously reported a reliable method for the isolation of intestinal crypts from the small intestine and robust three-dimensional (3D) expansion of intestinal organoids (basal-out) in adult bovines. The present study aimed to establish next-generation intestinal organoids for practical applications in disease modeling-based host-pathogen interactions and feed efficiency measurements. In this study, we developed a rapid and convenient method for the efficient generation of intestinal organoids through the modulation of the Wnt signaling pathway and continuous apical-out intestinal organoids. Remarkably, the intestinal epithelium only takes 3-4 days to undergo CHIR (1 µM) treatment as a Wnt activator, which is much shorter than that required for spontaneous differentiation (7 days). Subsequently, we successfully established an apical-out bovine intestinal organoid culture system through suspension culture without Matrigel matrix, indicating an apical-out membrane on the surface. Collectively, these results demonstrate the efficient generation and next-generation of bovine intestinal organoids and will facilitate their potential use for various purposes, such as disease modeling, in the field of animal biotechnology.

의약외품 마스크의 표준 치수 규격 개발 (Designing of standard sizing system for hygienic masks)

  • 오설영;서동애
    • 복식문화연구
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    • 제30권4호
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    • pp.495-512
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    • 2022
  • This study aims to design a new sizing system for hygienic masks to protect against COVID-19-related respiratory disorders. The product sizes were collected from 70 commercially available hygienic masks, and 18 head measurements were obtained from the three-dimensional (3D) scan data of 2,048 men and women aged 20 to 69 years from the 6th Size Korea Survey. The statistical analysis was performed using the IBM Statistical Package for the Social Sciences (SPSS) 28.0 program. The "bitragion-subnasale arc" and "menton-sellion length" were chosen as the key body dimensions for the hygienic mask sizing system. The linear regressions with 18 3D head measurements were analyzed, and it was determined that the key body dimensions were useful for statistically predicting other 3D head measurements related to hygienic masks. A new sizing system was proposed for two types of masks, "masks with a tight fit" and "masks with a loose fit," taking into account the existing Korean Industrial Standards (KS) and the shortcomings of the sizes of hygienic masks on the market. The sizing system for tight-fitting masks consisted of the key body dimensions, with their sizes indicated by a pair of numbers. The sizing system for loose-fitting masks consisted solely of the bitragion-subnasale arc, with their sizes denoted by letters such as S, M, and L (denoting small, medium, and large, respectively). Future studies should consider this mask sizing system for different age groups, such as children and adolescents.

소피아 비조비티의 폴딩 기법을 활용한 제로 웨이스트 패션 디자인 (Zero-waste fashion design using Sophia Vyzoviti's folding technique)

  • 도간오큠;서미희;이연희
    • 복식문화연구
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    • 제30권4호
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    • pp.513-528
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    • 2022
  • The purpose of this study is to understand the concept of zero-waste design and to apply Sophia Vyzoviti's folding techniques to develop a zero-waste fashion design method that contributes to sustainable fashion design. In this study, we explore the method and characteristics of zero-waste fashion design based on the concept of folding described in Sophia Vyzoviti's book on folding techniques. Using the autonomy of Sophia Vyzoviti's folding technique, four changeable folding fashion designs were developed and produced, demonstrating zero-waste fashion design. The results were as follows. First, the development of fashion designs using Sophia Vyzoviti's folding techniques enabled the development and production of free and creative zero-waste fashion designs that were three-dimensional, continuous, fluid, and full of potential. Second, the production of zero-waste patterns was further developed into a transformable fashion design that can be used with geometric patterns. These folding techniques produced a fashion design method that could transform one piece of clothing, demonstration the potential for maintenance of creativity using a zero-waste design based on these folding techniques. Third, the double-faced fabric, Neoprene, was chosen as an appropriate material as it emphasizes the depth of folding with application of two colors and its cotton/polyester blend that is suitable for folding.

한복웨이브 프로젝트에 개발된 한국적 패션디자인의 특성 (Characteristics of Korean fashion design developed in the Hanbokwave project)

  • 박은주;이영주
    • 복식문화연구
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    • 제31권2호
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    • pp.228-247
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    • 2023
  • In this study, an empirical analysis was conducted on the use of Korean design elements in the Hanbokwave project in 2022 in order to identify the expressed fashion characteristics and define Korean style. A case study was conducted on 98 items of clothing representing 10 Hanbok designer brands, and an analysis card was developed and used that classifies the silhouette of each item in detail. The analysis showed that in addition to the traditional skirt and Jeogori, the four stages of simulakr's image were prevalent in various fashion items such as shirts, blouses, jackets, crop tops, vests, dresses, and pants. In the composition of sleeves, many western clothing methods such as set-in-sleeve use and adjustment, hardcover buttons, and zippers were used to fuse the three-dimensional structure of western clothing and elements of traditional Hanbok to redefine it as postmodernism. It was recognized as a modern fashion, such as the trend of mixing and matching tops and bottoms, not a skirt and Jeogori set, by layering traditional clothes without hesitation or using them as a dress. As for the silhouette of the bottoms, the A-line showed a high frequency, and the prominent shape was identified as a traditional element widely used in modern Hanboks along with the element of wrinkles. This study is thought to be used as practical data for design development for the globalization of K-fashion in the future.

평면 스크린의 한계를 벗어나기 위한 디지털 아트에서의 현실적 공간 표현에 대한 연구 (A Study on the Realistic Spatial Expression in Digital Art to Escape the Limitations of Flat Screens)

  • 정세빈;최두현;임수연
    • 문화기술의 융합
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    • 제9권2호
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    • pp.105-112
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    • 2023
  • 3차원의 세계를 작품으로 구현해내려는 시도는 시대와 매체를 구분하지 않고 계속되어왔으며, 그 목적은 현실의 재현보다도 몰입 상태의 구현이다. 본 연구는 디지털미디어아트에서 최종적으로 관람자와 마주하는 매체인 평면스크린이 완전한 몰입을 방해한다고 보고, 스크린의 한계를 벗어나 공간의 현실감을 주는 작품들의 사례를 조사하고 분석하였다. 분석한 결과를 작품 제작에 활용한 결과, 디지털미디어아트 분야에서 현실적 공간의 제시와 몰입의 강화는 스크린 내의 시각적 영상 콘텐츠뿐만 아니라 전시 환경에 맞는 설치, 결과적으로 구성되는 공간 그 자체까지 작업으로 보아야 한다는 것을 알 수 있었다.

문학을 활용한 드라마의 전략 연구 -드라마 <날씨가 좋으면 찾아가겠어요>(2020)을 중심으로 (A Study of Strategies in Drama Using Literature -Centering on the drama <I will visit you if the weather is nice>(2020))

  • 손미영
    • 문화기술의 융합
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    • 제9권6호
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    • pp.771-777
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    • 2023
  • 본 연구는 드라마 <날씨가 좋으면 찾아가겠어요>(2020)을 통해서 드라마가 문학 작품을 활용하여 드라마의 정서와 서사, 그리고 인물을 효과적으로 제시하는 전략을 살핀다. 이 드라마는 시를 활용하여 인물의 내적 갈등을 효과적으로 보여주어 시청자들로 하여금 인물을 훨씬 더 긴밀하게 이해할 수 있도록 한다. 또한 동화의 서사를 통해 인물들 사이의 관계 변화와 성장의 서사를 보여준다. 한편으로는 회차마다 등장인물의 일기를 스틸컷과 함께 보여주어서 마치 드라마를 시청하는 것이 한 편의 문학 작품을 읽는 것과 같은 서정적인 효과를 불러일으킨다. 이로써 드라마 <날씨가 좋으면 찾아가겠어요>는 문학을 적극적으로 활용하여 시청자들로 하여금 성장과 치유의 서사를 공감하고 자신의 것으로 체험하게 하며, 문학이 지닌 치유의 효과를 입체적으로 형상화한다.

Current technology and industrialization status of cell-cultivated meat

  • Seung Yun Lee;Da Young Lee;Seung Hyeon Yun;Juhyun Lee;Ermie Jr Mariano;Jinmo Park;Yeongwoo Choi;Dahee Han;Jin Soo Kim;Sun Jin Hur
    • Journal of Animal Science and Technology
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    • 제66권1호
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    • pp.1-30
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    • 2024
  • Interest and investment in cultivated meat are increasing because of the realization that it can effectively supply sufficient food resources and reduce the use of livestock. Nevertheless, accurate information on the specific technologies used for cultivated meat production and the characteristics of cultivated meat is lacking. Authorization for the use of cultivated meat is already underway in the United States, Singapore, and Israel, and other major countries are also expected to approve cultivated meat as food once the details of the intricate process of producing cultivated meat, which encompasses stages such as cell proliferation, differentiation, maturation, and assembly, is thoroughly established. The development and standardization of mass production processes and safety evaluations must precede the industrialization and use of cultivated meat as food. However, the technology for the industrialization of cultivated meat is still in its nascent stage, and the mass production process has not yet been established. The mass production process of cultivated meat may not be easy to disclose because it is related to the interests of several companies or research teams. However, the overall research flow shows that equipment development for mass production and cell acquisition, proliferation, and differentiation, as well as for three-dimensional production supports and bioreactors have not yet been completed. Therefore, additional research on the mass production process and safety of cultivated meat is essential. The consumer's trust in the cultivated meat products and production technologies recently disclosed by some companies should also be analyzed and considered for guiding future developments in this industry. Furthermore, close monitoring by academia and the government will be necessary to identify fraud in the cultivated meat industry.

실내공간의 기호학적 공간분석에 관한 연구 -그레마스의 기호사변형을 중심으로- (A Study on the Analysis method of interior Space by Semiotic Approach)

  • 박진배;이수영;조종현
    • 한국실내디자인학회논문집
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    • 제16호
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    • pp.29-35
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    • 1998
  • The purpose of this study is to analyze the elements forming interior design and to examine dimensional relationship among the elements which form space through the comparison of the spatial language and semiotics of space for the component of interior design. In addition to that it indtends to derive the principle of design which dominate interior design and the inherent diversified meaning by comparing those elements with the square of semiotic used in semiotics. Through this comparsion the meaning of constituent forming space which can be observed through the comparsion of square of semiotic has redefined flexbility among relational system of elements and this flexible concept make the scope of environment including human being broad and enriched. This study fist of all analyzes various phenomena of social culture review semiotics meta-learning and examines back theoretical ground of semiotics which is needed for space analysis. Second of all in the area of presenting an analysis tool for meaningful analysis this report introduces the square of semiotics which was invented,. A. J. Greimas in order to analyze the meaning of literary work and defind three categories of the progressive research method for the analysis of interior design and research itself. Finally as for the analysis of meaning for interior design this report sets the space and analyzed the space in accordance with the method and research procedure. being

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