• Asian-Australasian Journal of Animal Sciences
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• v.14 no.10
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• pp.1470-1476
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• 2001

Kou Woan Lao is an oriental-style dairy product, which is coagulated by milk-clotting enzyme from the culture filtrate of lao-chao. The product appears smooth, sweet, slightly wine flavour, and the flavour differs from yogurt. The aim of this study was to search for the optimum model to shorten the period of manufacture, and to improve the quality of Kou Woan Lao. A response surface design was used for studying the effects of addition of carrageenan, locust bean gum, and culture filtrate from lao-chao on the curd firmness, viscosity, and syneresis. Results indicated that the best rheological property, preservative quality and acceptability of Kou Woan Lao could be obtained by the combination of 0.22% carrageenan, 0.21 % locust bean gum and 12% culture filtrate from lao-chao. The curd firmness, viscosity and syneresis of resultant product were 29.3 g, 21,347.7 cps, and 8.92%, respectively and the microstructure of the curd revealed a relatively complete three-dimensional spider web-like structure.

• Journal of Conservation Science
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• v.3 no.1 s.3
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• pp.3-12
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• 1994

As a result of the great attention to the historical and cultural heritage, the achievements of the natural sciences are finding their way into museums. The necessity of preserving the treasures of the artistic and cultural heritage has brought into being a new alliance, an alliance between science and culture. The most sciences of cultural properties pertain mainly to improvement of the methods of identifications, preservation and restoration of historical monuments. Recently a new physical method has appeared that, in a sense, possesses universal capabilities. That method is holography, which successfully combines unique features making it possible not only to create three-dimesional optical replicas of real three -dimensional objects practically indistinguishable from the original but also to study them. We shall deal in this papper with its scientific principle and application for displaying, as well as on other potential applications.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.289-292
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• 2002

The corneal tissue consists of three layers : epithelium, stroma, and endothelium. Central cornea is a highly differentiated tissue whereas the limbus contains the epithelial stem cell. In the present study. we report the engineering of the three-dimensional reconstructed cornea derived from rabbit limbal epithelial and stromal cells. The differentiation degree of corneal stem cells were assessed in serum concentration and inoculation density of stromal cells. Optimal condition differentiation of corneal stem cells is achieved when 5% FBS was supplemented to culture medium and $1-2{\times}10^5$ cells/ml inoculation density of stromal cells.

• The Journal of the Korea Contents Association
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• v.9 no.11
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• pp.130-139
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• 2009

This paper introduces the secondary cultural productions of game fandom that have been underestimated. The participatory cultural activities of game users are broadly expanding owing to machinima technology based on game engine. It allows gamers to make the three dimensional digital animations simple and easy way. This paper studies that how simple game consumers change their role as producers through machinima. This study is focused on the relationship between polysemy of participatory Machinima and former fandom's the secondary culture studies using ethnographic research method. It is to clarify relationship between the game users' psychological motivation for culture practice and the machinimas. We verify practically the machinima's effects on fandom culture through analysing viewers' reaction using replies on the machinimas and then find out machinima is a very important tool for expanding the game culture realm as the main body.

• Journal of Environmental Science International
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• v.8 no.5
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• pp.575-586
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• 1999

From the environmental aspects, primary productivity of phytoplankton plays the most improtant role in enhancement of marine culture oyster production. This study may be divided into two branches; one is estimation of maximum oyster meat production per unit facility(Carrying Capacity) under the present enviromental conditions in Kamak Bay, the other is improvement of carrying from increase of primary productivity by changing the environmental conditions that cause not ot form an unfavorable environment such as the formation of oxygen deficient water mass using the eco-hydrodynamic model. By simulation of three-dimensional hydrdynamic model and ecosystem model, the comparison between observed and computed data showed good agreement. The results of sensitivity analysis showed that phytoplankton maximum growth rate was the most important parameter for phytoplankton and dissolved oxygen. The estimation of mean primary productivity of Wonpo, Kamak, Pyongsa, and Kunnae culture grounds in Kamak Bay during culturing period were 3.73gC/$m^2$/d, 2.12gC/$m^2$/d, 1.98gC/$m^2$/d, and 1.26gC/$m^2$/d, respectively. Under condition not ot form the oxygen deficient water mass, four times increasing of pollutants loading as much as the present loading from river increased mean primary productivity of whole culture grounds to 4.02gC/$m^2$/d. Sediment N, P fluxes that allowed for 35% increasing from the present conditions increased mean primary productivity of whole culture grounds to 3.65gC/$m^2$/d. Finally, ten times increasing of boundary loadings from the present conditions increased mean primary productivity of whole culture grounds to 3.95gC/$m^2$/d. The maximum oyster meat production per year and that of unit facility in actual oyster culture grounds under the present conditions were 6,929ton and 0.93ton, respectively. This 0.93ton/unit facility is considered to be the carrying capacity in study area, and if the primary productivity is increased by changing the environmental conditions, oyster production can be increased.

• Journal of Veterinary Science
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• v.24 no.1
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• pp.4.1-4.16
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• 2023

Background: In vitro culture of preantral follicles is a promising technology for fertility preservation. Objectives: This study aims to investigate an optimized three-dimensional (3D) fetal bovine serum (FBS)-free preantral follicle culture system having a simple and easy operation. Methods: The isolated follicles from mouse ovaries were randomly divided in an ultra-low attachment 96-well plates supplement with FBS or bovine serum albumin (BSA) culture or encapsulated with an alginate supplement with FBS or BSA culture. Meanwhile, estradiol (E₂) concentration was assessed through enzyme-linked immunosorbent assay of culture supernatants. The diameter of follicular growth was measured, and the lumen of the follicle was photographed. Spindle microtubules of oocytes were detected via immunofluorescence. The ability of oocytes to fertilize was assessed using in vitro fertilization. Results: The diameters were larger for the growing secondary follicles cultured in ultra-low attachment 96-well plates than in the alginate gel on days 6, 8, and 10 (p < 0.05). Meanwhile, the E2 concentration in the BSA-supplemented medium was significantly higher in the alginate gel than in the other three groups on days 6 and 8 (p < 0.05), and the oocytes in the FBS-free system could complete meiosis and fertilization in vitro. Conclusions: The present study furnishes insights into the mature oocytes obtained from the 3D culture of the preantral follicle by using ultra-low attachment 96-well plate with an FBS-free system in vitro and supports the clinical practices to achieve competent, mature oocytes for in vitro fertilization.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.6
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• pp.1234-1240
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• 2009

Culture is not only a factor of a life worthy of man, but also that of beauty and fluency of life,so it works as a key to show differences in the quality of life. Paying attention to culture, which plays a role to create new things, is a source of high-added value. The term of cultural contents was derived in21C, combining digital skills with art. We are going to reconstruct and develope cultural properties such as remains, pottery, pictures, as a way of restoration for cultural contents with the view of reinterpretation. In this paper, we reinterpreted the pictures which were based on three particular elements in Chosun Dinasty- poetry, handwriting, and picture, and we produced 3D objects after analyzing texts and images in multimedia works applied with source pictures. As a highlighted method of restoration for cultural contents, we produced the work which can be interacted and has three dimensional objects getting out of appreciating of plane images. We presented a method of informing our culture with 3D Mong-yu-do-won-do, which used traditional paintings by being improved user friendliness and accessibility.

• BMB Reports
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• v.45 no.3
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• pp.189-193
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• 2012

Cyst formation is a major characteristic of ADPKD and is caused by the abnormal proliferation of epithelial cells. Renal cyst formation disrupts renal function and induces diverse complications. The mechanism of cyst formation is unclear. mIMCD-3 cells were established to develop simple epithelial cell cysts in 3-D culture. We confirmed previously that Mxi1 plays a role in cyst formation in Mxi1-deficient mice. Cysts in Mxi1 transfectanted cells were showed by collagen or mebiol gels in 3-D cell culture system. Causative genes of ADPKD were measured by q RT-PCR. Herein, Mxi1 transfectants rarely formed a simple epithelial cyst and induced cell death. Overexpression of Mxi1 resulted in a decrease in the PKD1, PKD2 and c-myc mRNA relating to the pathway of cyst formation. These data indicate that Mxi1 influences cyst formation of mIMCD-3 cells in 3-D culture and that Mxi1 may control the mechanism of renal cyst formation.

• Journal of Digital Contents Society
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• v.16 no.1
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• pp.113-121
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• 2015

Artifact pattern representation of a method using the digital technology is largely are two digital rubbing technique and digital contrast technique. Digital rubbing technique is clearly with the naked eye to see the original prestigious and difficult to build a digital rubbing data patterns by scanning a prestigious and patterns carved into the artifacts in three dimensions without compromising the circle to represent the three-dimensional effect by contrast it to the objective value refers to a technique that can be represented in digital. Digital contrast technique can not be calculated if the depth information of the object due to the glassy film of artefacts in a three-dimensional surface scanning and the application of this technique refers to a realistic image mapped digital technique for generating digital image contrast. Digital rubbing technique is mainly useful to express the most prestigious and patterns expressed in pottery or tile and stone monument, etc. engraved or embossed, digital contrast techniques can be used effectively to artifacts such as magnetic or glass can not be applied to the digital rubbing technique.

• Journal of Korean Society of Rural Planning
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• v.25 no.4
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• pp.129-149
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• 2019

This study is to give a three-dimensional view of the concept of rurality and ruralness in terms of time-span, perceived object and awareness level. In the precedent studies ruralness or rurality has been approached as the category of usefulness resources called amenity. This overlooked the perceived representation, institutional reference and rurality of ideological values. Rurality appears as a cumulative representation of being rural from the past to the present, but ruralness exists as a desirable form or value for the future. Through this study, it has been analyzed that ruralness consists of six realms such as environment, community, self-reliance, aesthetics, enjoyable amenity and settlement, and of three or four sub-realms by each amounting to 20 in total. According to the vocabulary listing by the survey to 30 experts, rurality for the past-present is mainly imagined as of natural environment, agriculture, landscape, and community history, while ruralness for the future is frequently described as of communities, settlement and self-reliance by number of references. Through the vocabulary extraction, 17 words in the level of mid-conception are induced including ecology, comfort, history, agriculture, landscape, place, culture, convenience, etc. In conclusion the concept of ruralness along with rurality could be organized into three different layers of perception consisting of representation, norms and usefulness.