• 한국물환경학회지
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• 제33권4호
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• pp.449-459
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• 2017

In Geum River of Korea, three multi-purpose weirs were built at the downstream of Daecheong Reservoir during the Four Major River Restoration Project (FMRRP). The weirs have altered the hydraulic characteristics of the river, and consequently transformed the large areas of flowing ecosystem to deep and wide stagnant environment. In every summer, a thermal stratification occurred near the Baekje Weir having mean depth of 4.0 m, and the surface algal blooms dominated by buoyant cyanobacteria have been frequently formed after the FMRRP. The objective of this study was to investigate the relationship between flow velocity and thermal stability of the waterbody using a three-dimensional (3D) hydrodynamic model (EFDC+) after calibration against the thermistor chain data obtained in 2014. A new Sigma-Zed vertical grid system of EFDC+ that minimize the pressure gradient errors was used to better simulate the thermodynamics of the waterbody. The model reasonably simulated the vertical profiles of the observed water temperatures. The vertical mean flow velocity and the Richardson Number (Ri) that represents the stability of waterbody were estimated for various management water levels and flow rates scenarios. The results indicated that the thermal stability of the waterbody is mostly high ($Ri{\gg}0.25$) enough to establish stratification, and largely depend on the flow velocity. The critical flow velocity that can avoid a persistent thermal stratification was found to be approximately 0.1 m/s.

• Journal of Microbiology and Biotechnology
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• 제18권1호
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• pp.28-34
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• 2008

Biofilm formation in association with the intercellular adhesion (icaADBC) gene cluster is a serious problem in nosocomial infections of Staphylococcus aureus. In all 112 S. aureus strains tested, the ica genes were present, and none of these strains formed biofilms. The biofilm formation is known to be changeable by environmental factors. We have found about 30% of phase variation in these strains with treatment of tetracycline, pristinamycin, and natrium chloride. However, this phenotype disappeared without these substances. Therefore, we have constructed stable biofilm-producing variants through a passage culture method. To explain the mechanism of this variation, nucleotide changes of ica genes were tested in strain S. aureus 483 and the biofilm-producing variants. No differences of DNA sequence in ica genes were found between the strains. Additionally, molecular analysis of three regulatory genes, the accessory gene regulator (agr) and the staphylococcal accessory regulator (sarA), and in addition, alternative transcription factor ${\sigma}^B$ (sigB), was performed. The data of Northern blot and complementation showed that SigB plays an important role for this biofilm variation in S. aureus 483 and the biofilm-producing variants. Sequence analysis of the sigB operon indicated three point mutations in the rsbU gene, especially in the stop codon, and two point mutations in the rsbW gene. This study shows that this variation of biofilm formation in S. aureus is deduced by the role of sigB, not agr and sarA.

• Nuclear Engineering and Technology
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• 제36권1호
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• pp.24-35
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• 2004

In order to analyze the hydrogen distribution during a severe accident in the APR1400 containment, GASFLOW II was used. For the APR1400 NPP, a hydrogen mitigation system is considered from the design stage, but a fully time-dependent, three-dimensional analysis has not been performed yet. In this study GASFLOW code II is used for the three-dimensional analysis. The first step to analysis involving hydrogen behavior in a full containment with the GASLOW code is to generate a realistic geometry model, which includes nodalization and modeling of the internal structures such as walls, ceilings and equipment. Geometry modeling of the APR1400 is conducted using GUI program by overlapping the containment cut drawings in a graphical file format on the mesh view. The total number of mesh cells generated is 49,476. And the calculated free volume of the APR1400 containment by GASFLOW is almost the same as the value from the GOTHIC modeling. A hypothetical SB-LOCA scenario beyond design base accident was selected to analyze the hydrogen behavior with the hydrogen mitigation system. The source of hydrogen and steam for the GASFLOW II analysis is obtained from a MAAP calculation. Combustion pressure and temperature load possibilities within the compartments used in the GOTHIC analysis are studied based on the Sigma-Lambda criteria. Finally the effectiveness of HMS installed in the APR1400 containment is evaluated from the point of severe accident management

• Bulletin of the Korean Chemical Society
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• 제8권2호
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• pp.69-72
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• 1987

The structures of dehydrated $Ag_9Cs_3$-A treated with hydrogen gas at three different temperatures have been determined by single-crystal X-ray diffraction techniques. Their structures were solved and refined in the cubic space group Pm3m at 23(1) $^{\circ}C$. All crystals were ion exchanged in flowing streams of aqueous $AgNO_3$/$CsNO_3$ with a mole ratio 1:3.0 to achieve the desired crystal composition. The structures treated with hydrogen at $23^{\circ}C(a=12.288(1)\;{\AA})\;and\;310^{\circ}C(a=12.291(2)\;{\AA})$ refined to the final error indices R1 = 0.091 and R2 = 0.079, and 0.065 and 0.073, respectively, using the 216 and 227 reflections, respectively, for which I >3${\sigma}$(I). In both of these structures, eight $Ag^+$ ions are found nearly at 6-ring centers, and three $Cs^+$ ions lie at the centers of the 8-rings at sites of $D_{4h}$ symmetry. One $Ag^{\circ}atom$, presumably formed from the reduction of a $Ag^+$ ion by an oxide ion of a residual water molecule or of the zeolite framework during the dehydration process, is retained within the zeolite, perhaps in a cluster. In these two structures hydrogen gas could not enter the zeolite to reduce the $Ag^+$ ions because the large $Cs^+$ ions blocked all the 8-windows. However, hydrogen could slowly diffuse into the zeolite and was able to reach and to reduce about half of the $Ag^+$ ions in the structure only at high temperature ($470^{\circ}C$). The silver atoms produced migrated out of the zeolite framework, and the protons generated led to substantial crystal damage.

• 한국광학회지
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• 제17권5호
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• pp.420-429
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• 2006

곡률, 두께, 굴절률 오차와 같은 광축대칭 오차요인들 때문에 상면이 이동하는 디지털 카메라용 줌렌즈 광학계에서 보상자를 이동시켜서 상면을 고정시키는 조건을 가우스 괄호법을 이용하여 유도하였다. 그리고 줌렌즈 광학계의 일반적인 세 가지 형태(3군, 4군, 5군 줌렌즈)를 사용하여 이 조건의 타당성을 검증하였다. 이러한 줌렌즈 형태에서 각 면의 곡률, 두께, 굴절률 등을 공차 내에서 Monte Carlo 방법에 따라 임의로 변화시켰을 때, 보상자의 이동량의 분포가 정규분포를 이루는 것을 확인하였다. 그리고 이로부터 공정능력을 계산하여 보상자의 최대 이동량에서 이에 따른 불량률을 이론적으로 예측하는 방법을 제시하였다.

• 대한의생명과학회지
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• 제29권1호
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• pp.48-52
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• 2023

Formaldehyde use is associated with serious health risks, which can affect medical personnel and technicians. Therefore, we investigated the efficacy of an alternative fixative, with respect to two types of formalin fixatives, by hematoxylin and eosin (H&E) staining, periodic acid Schiff (PAS) staining, immunohistochemical (IHC) staining, and RNA extraction. For H&E staining, the circular nucleus was stained dark blue by the basic dye hematoxylin and the cytoplasm was stained red by the acid dye eosin in all three fixative samples. No difference was found in the Duksan General Science (DGS), Sigma-Aldrich, and Core-Fix fixative samples (Corebiotech) used to fix kidney tissue, after PAS staining. IHC staining showed that CD4 was significantly increased in the lippolysaccharide (LPS)-treated group compared to the control group (vehicle), confirming the changes in specific molecules. The quantity and quality of RNA from tissues fixed in the three types of fixatives were evaluated. The average concentration of RNA was 106 ng/µL and average purity at A 260/280 ratio was 1.7~2.0, regardless of fixative used. For quality of protein, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was confirmed by Western blotting. In conclusion, Core-Fix can be used as a fixative for pathological tissues, in histological and molecular diagnoses.

• Bulletin of the Korean Chemical Society
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• 제26권7호
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• pp.1090-1096
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• 2005

$Ag_4Br_4$ nanoclusters have been synthesized in about 75% of the sodalite cavities of fully $K^+$-exchanged zeolite A (LTA). An additional KBr molecule is retained in each large cavity as part of a near square-planar $K_4Br^{3+}$ cation. A single crystal of $Ag_{12}$-A, prepared by the dynamic ion-exchange of $Na_{12}$-A with aqueous 0.05 M $AgNO_3$ and washed with $CH_3OH$, was placed in a stream of flowing 0.05 M KBr in $CH_3OH$ for two days. The crystal structure of the product ($K_9(K_4Br)Si_{12}Al_{12}O_{48}{\cdot}0.75Ag_4Br_4$, a = 12.186(1) $\AA$) was determined at 294 K by single-crystal X-ray diffraction in the space group Pm m. It was refined with all measured reflections to the final error index $R_1$ = 0.080 for the 99 reflections for which $F_o\;{\gt}\;4_{\sigma}\;(F_o)$. The thirteen $K^+$ ions per unit cell are found at three crystallographically distinct positions: eight $K^+$ ions in the large cavity fill the six-ring site, three $K^+$ ions fill the eight-rings, and two $K^+$ ions are opposite four-rings in the large cavity. One bromide ion per unit cell lies opposite a four-ring in the large cavity, held there by two eight-ring and two six-ring $K^+$ ions ($K_4Br^{3+}$). Three $Ag^+$ and three $Br^-$ions per unit cell are found on 3-fold axes in the sodalite unit, indicating the formation of nano-sized $Ag_4Br_4$ clusters (interpenetrating tetrahedra; symmetry $T_d$; diameter ca. 7.9 $\AA$) in 75% of the sodalite units. Each cluster (Ag-Br = 2.93(3) $\AA$) is held in place by the coordination of its four $Ag^+$ ions to the zeolite framework (each $Ag^+$ cation is 2.52(3) $\AA$ from three six-ring oxygens) and by the coordination of its four $Br^-$ ions to $K^+$ ions through six-rings (Br-K = 3.00(4) $\AA$).

• 한국광물학회지
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• 제6권2호
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• pp.116-121
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• 1993

제올라이트 $Na_{78}Rb_{28}-X$ 단결정을 $Na_{92}-X(Na_{92}Si_{100}Al_{92}O_{384})$을 $350^{\circ}C$에서 0.1Torr의 Rb 증기로 처리하여 만들어서 그 구조를 X-선 단결정법으로 공간군 Fd3로서 결정하였다 (${\alpha}=25.045(4){\AA}$). 구조는 I>$3{\sigma}(I)$인 회절반점 353개를 사용하여 최종오차지수 $R_1=0.082$, $R_2=0.084$까지 정밀화하였다. 단위포당 92개의 $Na^+$ 이온 중 대략 28개의 $Na^+$ 이온이 환원되고, 환원되어 생성된 28개의 Na' 중 약 14개만 제올라이트 골조내에 잔류하고 있다. 소다라이트 동공내에 사면체의 대칭성 ($T_d$)을 가지고 결합각 $109.5^{\circ}$, 결합거리 $2.80(2){\AA}$를 갖는다. $Na_5{^{4+}}$ 클라스트를 이루는 4개의 말단 원자와 골조 산소원자 사이의 결합거리는 $2.80(2){\AA}$이다. 몇몇 이중 6-링의 중심에 $Na_5{^{4+}}$ 클라스트 두 개를 선형으로 연결하여 지그재그형의 덩어리로 만드는 $Na^0$원자가 있다. 소다라이트 동공 중심과 이중 6-링에 있는 $Na^0$원자의 비편재화된 전자가 $Na_5{^{4+}}$ 클라스트를 안정화시키고 있다.

• 암석학회지
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• 제10권2호
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• pp.95-105
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• 2001

경기육괴의 3개지역에서 변성암 시료를 채취하여 저어콘 U-Pb 법에의한 연대측정을 실시하였다. 각 지역의 암석에서 분리한 저어콘들이 만드는 불일치선과 일치곡선의 위와 아래 교점에서 구한 연대를 살펴보면 용두리 편마암 복합테의 경우 $2168\pm$24 Ma와 $1227\pm$40 Ma, 의암층군의 경우$ 1955\pm$22 Ma와 $493\pm$32 Ma,그리고 용문산 충군의 경우는 $3712\pm$244 Ma와 $1613\pm$51 Ma로 계산된다(오차는 모두 $2\sigma$). 용두리 변성암 복합체와 의암층군의 저어콘 U-Pb 연대는 각각 영남육괴의 화강암질 편마암 및 반성변정질 편마암으로부터 측정된 저어콘 U-Pb 연대와 매우 유사하다. 이러한 유사성은 경기와 영남의 두 육괴가 약 22~19억년의 시기에 비슷한 지구조 및 지리적 환경에 있었을 가능성을 제기한다 용문산충군의 경우 대부분의 저어콘들이 아래교점 가까이 놓이기 때문에 37억년의 상부교점 연대는 큰 오차를 보이며 검증이 필요하다. 그러나 이 연대는 최근 중국 북동부 지역에서 보고된 것과 유사한 동북아시아에서 가장 오래된 지각이 한반도에 존재하였을 가능성을 시사하기 때문에 매우 중요한 의미를 갖는다고 판단된다. 용문산충군 아래교점의 연대는 강한 변성작용의 시기를 나타내는 것으로 해석된다. 이 시기는 영남육괴의 18.5~17억년의 변성작용 및 화성활동 시기보다는 약간 후기이며, 두 육괴에 동시에 일어났던 고원생대말 지구조적 변혁의 말기활동을 기록하는 연대 일 가능성이 있다.

• 대한방사선기술학회지:방사선기술과학
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• 제40권1호
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• pp.63-70
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• 2017

본 연구는 세기변조방사선 치료기술을 이용한 성문암 환자에게 사용되는 두 가지 상이한 열가소성 고정기구 마스크로 인한 환자위치잡이 오차를 비교 평가하고 자 하였다. 치료가 종료된 총 16명의 성문암 환자를 두 그룹으로 나누었고(기존마스크 vs. 변형마스크), 평균, 3D오차, 시스템과 랜덤오차를 구하여 환자위치잡이 오차를 비교하였다. 또한, 치료계획종양체적(PTV)에 대한 여백(margin)을 분석하였다. 3D오차에 대하여 기존 그룹은 $5.2{\pm}1.3mm$고, 변형그룹은 $5.9{\pm}0.7mm$로써, 변형마스크가 변형보다 13.6% 높았다. 시스템오차는 기존그룹(변형그룹)에서 좌표 x, y, z방향은 각각 1.7 mm (1.1 mm), 1.0 mm (1.8 mm), 1.5 mm (2.0 mm)였고, 회전각(roll angle)은 $0.8^{\circ}$ ($0.8^{\circ}$)였다. 랜덤오차는 변형그룹이 기존그룹에 비하여 좌표 x, y, z방향으로 10.9%, 1.7%, 23.1%로 낮았으나, 회전각은 12.4% 높았다. PTV여백에서 변형그룹은 좌표 x방향에 대하여 기존그룹에 비하여 31.8% 낮았으나, 반대로 좌표 y와 z방향에서는 기존그룹보다 각각 52.6%와 21.6%로 높았다. 성문암 세기변조방사선치료에서 변형된 마스크 사용은 고정기구의 변형으로 인한 환자위치잡이 오차는 수치적으로는 영향을 줄 수 있지만, 다양한 관점에서 고정기구 마스크에 대한 연구가 임상적인 측면에서 연구가 필요할 것으로 사료된다.