• Plant Biotechnology Reports
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• 제2권3호
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• pp.171-177
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• 2008

This paper reports an improved protocol for isolation, culture and regeneration of Lotus corniculatus protoplasts. A range of parameters which influence the isolation of L. corniculatus protoplasts were investigated, i.e., enzyme combination, tissue type, incubation period and osmolarity level. Of three enzyme combinations tested, the highest yield of viable protoplasts was achieved with the combination of 2% Cellulase Onozuka RS, 1% Macerozyme R-10, 0.5% Driselase and 0.2% Pectolyase. The use of etiolated cotyledon tissue as a source for protoplast isolation proved vital in obtaining substantially higher protoplast yields than previously reported. Culture of the protoplasts on a nitrocellulose membrane with a Lolium perenne feeder-layer on the sequential series of PEL medium was highly successful in the formation of microcolonies with plating efficiencies 3-10 times greater than previous studies. Shoot regeneration and intact plants were achieved from 46% of protoplast-derived cell colonies.

• International Journal of Concrete Structures and Materials
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• 제8권1호
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• pp.43-59
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• 2014

Many bridges are subject to lateral damage for their girders due to impact by over-height vehicles collision. In this study, the optimum configurations of carbon fiber reinforced polymers (CFRP) laminates were investigated to repair the laterally damaged prestressed concrete (PS) bridge girders. Experimental and analytical investigations were conducted to study the flexural behavior of 13 half-scale AASHTO type II PS girders under both static and fatigue loading. Lateral impact damage due to vehicle collision was simulated by sawing through the concrete of the bottom flange and slicing through one of the prestressing strands. The damaged concrete was repaired and CFRP systems (longitudinal soffit laminates and evenly spaced transverse U-wraps) were applied to restore the original flexural capacity and mitigate debonding of soffit CFRP longitudinal laminates. In addition to the static load tests for ten girders, three more girders were tested under fatigue loading cycles to investigate the behavior under simulated traffic conditions. Measurements of the applied load, the deflection at five different locations, strains along the cross-section height at mid-span, and multiple strains longitudinally along the bottom soffit were recorded. The study investigated and recommended the proper CFRP repair design in terms of the CFRP longitudinal layers and U-wrapping spacing to obtain flexural capacity improvement and desired failure modes for the repaired girders. Test results showed that with proper detailing, CFRP systems can be designed to restore the lost flexural capacity, sustain the fatigue load cycles, and maintain the desired failure mode.

• 대한한의진단학회지
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• 제10권1호
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• pp.46-53
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• 2006

Magnetocardiography provides non-invasive three-dimensional electroanatomical imaging of cardiac electrophysiology. The aim of this study is to find the index for using the indicator of magnetocardiography. An object of this study is 214 papers registered on www.pubmed.com. The index used in magnetocardiography is the interval, morphology, amplitude and integrals of QRS complex, P wave, T wave. The current density and electric field map are used, too. A new try of the index is LAS, W-HEAD, Relative Smoothness index(RS index) etc.

• 식물병연구
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• 제19권4호
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• pp.308-312
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• 2013

The popcorn disease caused by sclerotia forming fungi reduces the productivity of mulberry fruits in world wide. In Korea, only two species (Ciboria shiraiana and Scleromitrula shiraiana) have been reported as the major causal organisms and their morphological features are also largely unknown. Hereby, we report the first identification of another species (i.e. Ciboria carunculoides) in Korea and detailed features of their anamorphic stage. Fungi dominantly associated with sclerotia were purely isolated from infected mulberry fruits under the microscope. PCR-amplified DNA encoding 5.8S rRNA displayed 100% similarity to Ciboria carunculoides. The anamorphic features exhibited the absence of true mycelia. Instead, very short, aseptated, branched conidiophores were directly emerged from sclerotia. Phialides were usually three in number from each conidiophore, ampuliform to navicular in shape, slightly curved and tapering towards the apex. Conidia were produced from phialides and mostly found as one celled, pear shaped, not hyaline with smooth to uneven surface walled. Diversely modified features in phialides formed pseudo-mycelial structures around the host tissue. Combined all, current study is the first report of C. carunculoides isolated in Korea and the foremost detailed description of its anamorph stage.

• Journal of Chest Surgery
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• 제17권4호
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• pp.657-665
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• 1984

Thirty-seven patients had undergone repair of a endocardial cushion defect between 1977 and Aug. 1983 in Seoul National University Hospital. Twenty eight had a partial defect, one intermediate defect and eight complete endocardial cushion defect. Tricuspid cleft was found in 4 cases and mitral cleft was in all p-ECD. Seven patients were of type C anatomy in c-ECD. Four patients had associated major anomalies, including three TOF in c-ECD, one coarctation in p- ECD. In p-ECD patients, the septal defect was closed with patch in all cases and the atrioventricular valvular insufficiency was corrected with MVR in 4 cases, TVR in 1 case and simple interrupted sutures in remainders. In c-ECD patients the septal defect was closed with single patch except one case. The atrioventricular valve was repaired with simple interrupted sutures except one MVR and TVR case. The operative mortality was 14.2% in p-ECD, 44.4% in c-ECD, but recent 3 years [1980-1983] mortality was 8.7% in p-ECD, 20% in c-ECD. More than grade III systolic regurgitant murmur was oted postoperatively in 4 cases of c-ECD and 3 cases of p-ECD. The operative risk factors were preoperative NYHA classification, cyanosis, Rp/Rs, systolic pressure of main pulmonary artery and the degree of regurgitation of atrioventricular valves. The causes of death were low cardiac output syndromes, pulmonary complications and arrhythmias.

• Steel and Composite Structures
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• 제13권4호
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• pp.367-382
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• 2012

This paper is dedicated to the buckling behaviors of strengthened perforated plates under edge shear loading, which is a typical load pattern of steel plates in civil engineering, especially in plate and box girders. The square plates considered each has a centric circular hole and is simply supported on four edges in the out-of-plane direction. Three types of strengthening stiffeners named ringed stiffener (RS), flat stiffener (FSA and FSB) and strip stiffener (SSA, SSB and SSC) are mainly discussed. The finite element method (FEM) has been employed to analyse the elastic and elasto-plastic buckling behavior of unstrengthened and strengthened perforated plates. Results show that most of the strengthened perforated plates behave higher buckling strengths than the unstrengthened ones, while the enhancements in elastic buckling stress and elasto-plastic ultimate strength are closely related to stiffener types as well as plate geometric parameters including plate slenderness ratio and hole diameter to plate width ratio. The critical slenderness ratios of shear loaded strengthened perforated plates, which determine the practical buckling pattern (i.e., elastic or elasto-plastic buckling) of the plates, are also studied. Based on the contrastive analyses of strengthening efficiency considering the influence of stiffener consumption, the most efficient cutout-strengthening methods for shear loaded perforated square plates with different slenderness ratios and circular hole diameter to plate width ratios are preliminarily identified.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume I
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• pp.224-227
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• 2006

For estimation of three inherent optical properties (IOPs), the absorption coefficients for phytoplankton ($a_{ph}$) and suspended solid particle ($a_{ss}$) and dissolved organic matter ($a_{dom}$), from ocean reflectance, we used inversion of remote sensing reflectance model (Ahn et al., 2001) at this study. The IOP inversion model assumes that (1) the relationship between remote sensing reflectance ($R_{rs}$) and absorption (a) and backscattering ($b_{b}$) is well known, (2) the optical coefficients for pure water ($a_{w}$, $b_{bw}$) are known, (3) the spectral shapes of the specific absorption coefficients for phytoplankton ($a^*_{ph}$) and suspended solid particle ($a^*_{ss}$) and the specific backscattering coefficients for phytoplankton ($b_b^*_{ph}$) and suspended solid particle ($b_b^*_{ss}$) are known. The input data of IOP inversion model is used in-situ ocean optical data at the seawater around the Korea Peninsula for 5 years (2001-2005). We compared the output data of the IOP inversion model and the in-situ observation for seawater around the Korea Peninsula.

• 마이크로전자및패키징학회지
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• 제19권4호
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• pp.45-50
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• 2012

A family of multi-die DRAM packages was developed that incorporate the full functionality of an SODIMM into a single package. Using a common ball assignment analogous to the edge connector of an SODIMM, a broad range of memory types and assembly structures are supported in this new package. In particular DDR3U, LPDDR3 and DDR4RS are all supported. The center-bonded DRAM use face-down wirebond assembly, while the peripherybonded LPDDR3 use the face-up configuration. Flip chip assembly as well as TSV stacked memory is also supported in this new technology. For the center-bonded devices (DDR3, DDR4 and LPDDR3 ${\times}16$ die) and for the face up wirebonded ${\times}32$ LPDDR3 devices, a simple manufacturing flow is used: all die are placed on the strip in a single machine insertion and are sourced from a single wafer. Wirebonding is also a single insertion operation: all die on a strip are wirebonded at the same time. Because the locations of the power signals is unchanged for these different types of memories, a single consolidated set of test hardware can be used for testing and burn-in for all three memory types.

• 한국보육지원학회지
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• 제16권5호
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• pp.69-86
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• 2020

Objective: The purpose of this research was to examine the prediction models of conflict and intimacy in teacher-child relationships based on decision tree analysis. Methods: The participants were 297 preschool children from ages three to five including 166 boys and 131 girls. Teacher-child relationships were measured by the Student-Teacher Relationship Scale(STRS). Physical aggression, relational aggression, social withdrawal, and prosocial behaviors were measured by teacher ratings. Moreover, ADHD-RS(Attentive Deficit Hyperactivity Disorder Rating Scale) was used to measure ADHD. The data was analyzed with decision tree analysis. Results: According to the prediction model for teacher-child conflict, the significant predictors were physical aggression and social withdrawal. According to the prediction model for teacher-child intimacy, the significant predictors were prosocial behaviors and relational aggression. However, children's age, gender and ADHD were not significant predictors. Conclusion/Implications: The findings suggest that social behaviors may be closely related with teacher-child relationships for preschool children. Based on the results of this study, intervention suggestions were made.

• International Journal of Precision Engineering and Manufacturing
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• 제6권3호
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• pp.45-50
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• 2005

This paper proposed an efficient manufacturing system using the OMM (on-machine measurement) system. The OMM system is software-based 3D modeler for inspection on machine, and it is interfaced with machine tools via RS232C. The software is composed of two inspection modules; one is touch probe operating module, and the other is laser displacement sensor operating module. The module for touch probe needs the inspection feature extracted from CAD data. The touch probe moves to workpiece by three operating modes as follows: manual, general and automatic mode. The operating module of the laser displacement sensor is used to inspect profiles and very small holes. An advantage of this inspection method is the ability to execute on-line inspection during machining or afterward. The efficiency of proposed system which can predict and define the machining errors of each process was verified, so the developed system was applied to inspect a mold-base (cavity, core).