• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.2745-2749
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• 2015

Objective: To study the PLCE1 gene rs2274223 polymorphism with regard to esophageal cancer and its interaction with diet, lifestyle, psychological and environmental factors in Southwest Shandong province. Materials and Methods: A case series study (case-case) was conducted. Questionnaire data were collected and 3 ml-5ml venous blood was drawn for DNA extraction among the qualified research subjects. PLCE1 gene polymorphism was detected after PCR amplification of DNA. SPSS 13.0 software was used for statistical analysis of the data. Results: The three genotypes A/A, A/G and G/G PLCE1 gene rs2274223 was 31, 16 and 4 cases, accounting for 60.8%, 31.4%, 0.08% respectively. The difference of three genotypes (AA/GA/GG) proportion between negative and positive family history of patients was statistically significant, ${\chi}^2=6.213$, p=0.045. There was no statistically significant relationship between PLCE1 gene rs2274223 polymorphism and smoking, drinking, ${\chi}^2=0.119$, p=0.998, and ${\chi}^2=1.727$, p=0.786. There was no linkage of the three rs2274223 PLCE1 gene genotypes (AA/GA/GG) proportion with eating fried, pickled, hot, mildew, overnight, smoked, excitant food, eat speed, salt taste or not (p>0.05). or with living environment pollution and nine risk factors of occupational exposure (p>0.05). There was no statistically significant difference in TS scores between different genotype of rs2274223 PLCE1 gene. Conclusions: The PLCE1 rs2274223 polymorphism has a relationship with family history of esophageal cancer, but does not have any significant association with age, gender, smoking, alcohol drinking, food hygiene, eating habits, living around the environment and occupation in cases.

• Asian Pacific Journal of Cancer Prevention
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• 제15권8호
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• pp.3811-3815
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• 2014

Background: Colorectal cancer (CRC) is the worldwide disease which causes enormous losses every year. Recent studies suggested that environmental and gene factors might be the etiologies in increasing the risk of morbidity. Nitric oxide synthase 3 (NOS3) gene polymorphisms are said to be associated with CRC risk but the conclusion is still controversial. Materials and Methods: Pubmed and HuGENet databases up to December 2013 were used in this meta-analysis. Three different certain genotypic models were applied, namely dominant (AA+AC versus CC), recessive (AA versus AC+CC), per-allele analysis (A vs C). In addition, information on tumor sites and pathologic stages was collected. The strength of associations was assessed through combining odds ratio (OR) and 95% confidence interval (CI). Results: Finally, five and three studies about the rs1799983 and rs2070744 were covered in the analysis with 2,745 cases and 2,478 controls. Three models were applied, but no significant association was found for NOS3 G894T/rs1799983 (dominant: OR=0.999, 95%CI=0.797-1.253, $I^2$=63.8%; recessive: OR=0.924, 95%CI=0.589-1.450, $I^2$=59.3%; allele analysis: OR=0.979, 95%CI=0.788-1.216, $I^2$=74.9%) and T-786C/rs2070744 (dominant: OR=1.138, 95%CI=0.846-1.530, $I^2$=67.9%; recessive: OR=0.956, 95%CI=0.708-1.291, $I^2$=0.0%; allele analysis: OR=1.110, 95%CI=0.865-1.425, $I^2$=69.4%). The same results were also obtained for tumor sites and pathologic stage subgroups. After further analyzing the NOS3 gene, rs1799983 as the tag- and functional SNP was presented. Conclusions: On the basis of this meta-analysis and the characteristics of the NOS3 gene, we suggested rs1799983 might be a key locus associated with CRC risk. Further prospective studies were needed to make more comprehensive explanation of the associations.

• 인터넷정보학회논문지
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• 제12권5호
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• pp.21-28
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• 2011

최근 들어 전도성 섬유를 사용한 의료 및 엔터테인먼트 관련 어플리케이션들이 제안되고 있지만, 전도성 섬유를 이루는 전도성사의 성능에 대한 기본 자료는 그 실을 만들어내는 회사가 제시하는 몇 가지 특성 말고는 통신을 위해 필요한 특성이 정립되어 있지도 않고 검증되어 있지도 않은 상태이다. 따라서 전도성사가 통신에 사용될 때 어느 정도의 통신성능을 보이는 지 검증하고, 기존의 통신 프로토콜들이 전도성사를 이용한 통신에서도 적용될 수 있을지, 아니면 새로운 프로토콜이 개발되어야 할 지 가이드라인이 필요하다. 본 논문에서는 국내 및 국외의 전도성사의 특성을 취합하여 분류해 보고, 가장 간단한 통신 프로토콜인 RS232 등의 직렬 통신 프로토콜들을 이용해 각 전도성사들의 전도 효율성을 실험하여 그 성능을 알아보았다. 국내 및 국외의 6가지 실에 대해 0.5m, 1m, 2m, 3m 길이에 대해 통신 성능을 실험하였으며, 데이터 전송률과 에러율을 측정하였다. 통신 프로토콜로는 RS485가 모든 부류의 전도성사에 대해 데이터를 전송하는 우수한 전도특성을 나타냈으나 거리에 비례하여 에러율이 높아지게 되어 이론상 12km까지 장거리 전송용으로 사용될 수 있는 RS485의 특성을 살릴 수 없었다. 아예 전도 기능을 보이지 않은 실들을 제외한 다른 전도성사들에 대해서는 RS232가 에러 없이 가장 안정적인 처리율을 보였다. USB와 같은 고속 통신 프로토콜도 전도성사를 이용한 데이터 전송에 활용될 수 있음이 확인되었으나, 전송가능 거리가 최대 2m를 넘는 거리에서는 아예 전도성사를 통해 통신이 이루어지지 않았다. 또한 USB 프로토콜은 전원까지 전도성사를 사용해 공급하는 경우 매우 불안한 동작을 보여, 전원공급 문제가 우선적으로 해결되어야 함을 알 수 있었다. 본 연구는 추후 전도성사를 이용하는 통신 프로토콜의 개발과 통신용 전도성 섬유 개발 시 고려해야 할 요구사항의 기본 데이터로 활용될 수 있을 것이다.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 춘계학술대회 논문집
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• pp.365-368
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• 2006

국내 해빈지대의 경우, 인위적 개발과 기상학적 현상, 특히 태풍의 영향으로 다량의 모래가 유실되고 있다. 해안침식 종합대책 수립 및 사업실행에 있어 해안지형에 관한 정확한 자료 구축은 필히 요구된다. 그러나 현재까지, 연안에 관한 정확한 측량작업이 실행되지 않은 채, 모래 유실에 대한 문제 제기나 일시적 대책 마련에 급급하고 있다. 그러므로 본 연구에서는 연구지역을 해운대 해빈지역으로 선정하고, GPS 측량기법을 이용하여 해안지대의 정확한 공간자료를 구축하였으며, 태풍으로 인한 3차원 지형변화를 정밀하게 분석하였다. 연구결과, 태풍의 영향으로 호안벽 근처 해빈고도는 증가하였으나, 강풍과 북북동의 정온입사파로 $2320m^3$ 가량 해빈이 침식하였다. 특히 동측 해빈지대에서 침식현상이 뚜렷하게 발생하였다. RS, GPS, GIS 기법을 기반으로 해안지대의 정확한 공간DB 구축과 지형변화에 관한 정량적정성적 분석 작업은 국내 해안침식에 관한 체계적이며 효과적인 대책을 수립하는데 도움을 줄 수 있을 것으로 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권4호
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• pp.2199-2203
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• 2016

One of the most common cancer types faced by the women around the world is breast cancer. Among the several low, moderate and high penetrance genes conferring susceptibility to breast cancer, PTEN is one which is known to be mutated in many tumor types. In this study, we predicted and analyzed the impact of three deleterious coding non-synonymous single nucleotide polymorphisms rs121909218 (G129E), rs121909229 (R130Q) and rs57374291 (D107N) in the PTEN gene on the phenotype of breast tumors using computational tools SIFT, Polyphen-2, PROVEAN, MUPro, POPMusic and the GETAREA server.

• 대한산업공학회지
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• 제15권1호
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• pp.1-15
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• 1989

In the past few years, increasing numbers of automatic storage/retrieval system (AS/RS) using computer controlled storage/retrieval machine have been installed. This paper introduces two modeling approaches to determine the best operating policy for AS/RS : an M/G/1 queueing model and a computer simulation model. The operating policy consists of three elements. : the operation command cycle, the storage location method, and the operation dispatching rule. The analysis based on M/G/1 model is suitable for a quick and approximate evaluation, due to its inherent strict assumptions. The computer simulation can be used to perform a more realistic analysis. It is shown through the study that a significant improvement in the throughput and/or the space requirement can be expected by determining the best operating policy to a particular system. Most important, the computer simulation demonstrates its powerful capability in evaluating dynamic stochatic systems with imperfect information.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.133-133
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• 2003

Recently, sperm has been used as a vector to carry exogenous genes for the production of transgenic animals. However, the success in cattle is low, due to deficiencies in oocyte activation and sperm decondensation caused by high disulphide bond (S=S) content in mature sperm. This study was carried out to develop an effective method for producing transgenic animals with round spermatids (RS). Two methods of embryo production - electric fusion (EC) or intracyto-plasmic injection (IC) and three activation treatments were compared. RS were isolated from bull testes by Percoll density gradients (20, 35, 40, 45 and 90%). Fusion between ooplast and RS was performed with a single DC electric pulse (1.0 KV/cm, 45 sec) in 0.28 M mannitol solution supplemented with 100 M CaCl2 and 100 M MgCl$_2$. (중략)

• Asian Pacific Journal of Cancer Prevention
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• 제16권2호
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• pp.775-781
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• 2015

Background: RB1 (retinoblastoma 1) was reportedly one of the major determinative factors for sensitivity to taxanes in previous studies. In this study, we investigated the influence of RB1 single nucleotide polymorphisms (SNPs) on the efficacy of platinum-taxane regimens in advanced NSCLC patients. Materials and Methods: 234 cases of patients with advanced NSCLC who were treated with first-line platinum-taxane agents were enrolled in this study. Genomic DNA was extracted from patients' peripheral blood samples using a QIAamp DNA Maxi Kit, and genotyped by iSelect HD Bead-Chip. Results: Regression analyses were conducted through the univariate and multivariate Cox proportional hazards model in the 234 patients. The results showed that of the eight RB1 tagSNPs, only rs4151510 was a positive predictive factor for the advanced NSCLC patients treated with platinum taxanes regimen. The patients with G/G genotype of RB rs4151510 had longer overall survival (OS) than the non-G/G genotype (p=0.018). The histology was also correlated with OS in the whole advanced NSCLC patients. Three tagSNPs of RB1, rs4151510, rs4151465, rs9568036 were significantly associated with OS in the advanced NSCLC patients with squamous cell histology using Kaplan-Meier overall survival analysis stratified by histology. Conclusions: RB1 genomic variants were correlated with the efficacy of platinum-taxanes regimen. RB rs4151510 is an independent factor of the prognosis of NSCLC patients receiving platinum-taxane chemotherapy.

• Biomolecules & Therapeutics
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• 제26권2호
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• pp.130-138
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• 2018

Quercetin and resveratrol are known to have beneficial effects on the diabetes and diabetic complication, however, the effects of combined treatment of these compounds on diabetes are not fully revealed. Therefore, the present study was designed to investigate the combined antidiabetic action of quercetin (QE) and resveratrol (RS) in streptozotocin (STZ)-induced diabetic rats. To test the effects of co-treated with these compounds on diabetes, serum glucose, insulin, lipid profiles, oxidative stress biomarkers, and ions were determined. Additionally, the activities of hepatic glucose metabolic enzymes and histological analyses of pancreatic tissues were evaluated. 50 male Sprague-Dawley rats were divided into five groups; normal control, 50 mg/kg STZ-induced diabetic, and three (30 mg/kg QE, 10 mg/kg RS, and combined) compound-treated diabetic groups. The elevated serum blood glucose levels, insulin levels, and dyslipidemia in diabetic rats were significantly improved by QE, RS, and combined treatments. Oxidative stress and tissue injury biomarkers were dramatically inhibited by these compounds. They also shown to improve the hematological parameters which were shown to the hyperlactatemia and ketoacidosis as main causes of diabetic complications. The compounds treatment maintained the activities of hepatic glucose metabolic enzymes and structure of pancreatic ${\beta}-cells$ from the diabetes, and it is noteworthy that cotreatment with QE and RS showed the most preventive effect on the diabetic rats. Therefore, our study suggests that cotreatment with QE and RS has beneficial effects against diabetes. We further suggest that cotreatment with QE and RS has the potential for use as an alternative therapeutic strategy for diabetes.

• 대한의생명과학회지
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• 제15권1호
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• pp.31-35
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• 2009

High blood pressure (BP) is the most frequent risk factor among metabolic syndrome components. The control of hypertension is very important to prevent the cardiovascular risk in metabolic syndrome. The dysfunction of calcium channel is responsible in the regulation of the vascular muscle contribution to hypertension. Calcium channel, voltage-dependent, P/Q type, alpha-1A subunit (CACNA1A) gene is located in brain and known to control the intracranial hypertension. In this study, we investigate whether the polymorphisms of CACNA1A gene is associated with hypertension. The 49 CACNA1A genotypes were determined using the Affymetrix Genotyping chip array in 92 hypertension and 279 control individuals from a Korean population. Logistic and multiple regression models were employed to analyze the genetic contributions of polymorphisms. Out of 49 polymorphisms, six SNPs (rs12611029, rs16035, rs7259944, rs10419472, rs17777900, and rs4926294) showed a significant association with hypertension in three alternative models (codominant, dominant, and recessive models; P<0.05 after adjusting for age and sex). Our results suggest that the CACNA1A gene may be associated with hypertension in the Korean population.