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Electron microscopic studies on Flavobacterium branchiophila in experimentally induced gill disease of rainbow trout (세균성(細菌性) 아가미병(病)에 실험적(實驗的)으로 감염(感染)된 무지개송어에 있어서 Flavobacterium branchiophila에 대한 전자현미경학적(電子顯微鏡學的) 연구(硏究))

  • Heo, Gang-joon
    • Korean Journal of Veterinary Research
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    • v.32 no.3
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    • pp.381-387
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    • 1992
  • Gill epithelia of normal rainbow trout fingerlings and abnormal ones suffering bacterial gill disease by experimental infection were examined by transmitting electron microscopy (TEM) and scanning electron microscopy (SEM). TEM observations revealed that Flavobacterium branchiophila consisted of slender rods measuring 0.5 by 5 to $8{\mu}m$, and they had which were long, thin, flexible filaments measuring approximately 4 nm by $1{\mu}m$, and packed together to organize into bundles. Morphological alterations of the diseased epithelia started at hypertrophy of the lamellar epithelium. F branchiophila attached to the gill surface of infected fish through pili with a regular distance, and did not invade into gill tissue. In SEM observations, normal surface ultrastructure of epithelial cell in the outermost layer were characterized by a typical labyrinth-like structure branching and anastomosing microridges on the cell surface. Hyperplastic lesions in experimentally infected gill were most serious at near the tips. Each filament exhibited a club-like, and fusion between the filaments was sometimes observed at their tips. On the surface of gill filaments, thread-like bacterial cells attached and were entangled. The bacterial cells almost covered the surface. After immersion in 5 % NaCl, the cell of F branchiophila, however, appeared to be indeterminate shape.

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The Effect of the Water Extract of Uncariae Ramulus et Uncus on Gliosis in the Middle Cerebral Artery Occlusion(MCAO) Rats (조구등이 MCAO 모델 흰쥐에서 gliosis 억제에 미치는 영향)

  • Kim, Sang-Woo;Kim, Sun-Ae;Song, Bong-Keun
    • The Journal of Internal Korean Medicine
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    • v.31 no.4
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    • pp.763-774
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    • 2010
  • Objectives : In condition of brain infarction, irreversible axon damage occurs in central nerve system(CNS), because gliosis becomes physical and mechanical barrier to axonal regeneration. Reactive gliosis induced by ischemic injury such as middle cerebral artery occlusion is involved with up-regulation of GFAP and CD81. The current study is to examine the effect of the Uncariae Ramulus et Uncus on CD81 and GFAP expression in the rat brain following middle cerebral artery occlusion. Methods : In order to study ischemic injuries on brain, infarction was induced by middle cerebral artery occlusion(MCAO) using insertion of a single nylon thread, through the internal carotid artery, into a middle cerebral artery. Cresyl violet staining, cerebral infarction size measurement, immunohistochemistry and microscopic examination were used to detect the expression of CD81 and GFAP and the effect on the infarct size and pyramidal cell death in the brain of the rat with cerebral infarction induced by MCAO. Results : The following results were obtained 1. Measuring the size of cerebral infartion induced by MCAO in the rat after injection of Uncariae Ramulus et Uncus showed the size was decreased. 2. Intravenous injection of Uncariae Ramulus et Uncus showed pyramidal cell death protection in the hippocampus in the MCAO rat. 3. Water extract injection of Uncariae Ramulus et Uncus decreased GFAP expression significantly in the MCAO rat. 4. Uncariae Ramulus et Uncus water extract decreased CD81 expression in the MCAO rat. 5. The administration of water extract of Uncariae Ramulus et Uncus induced up-regulation of c-Fos expression significantly compared with MCAO. 6. The admistration of water extract of Uncariae Ramulus et Uncus increased ERK expression significantly compared with MCAO. Conclusion : We observed that Uncariae Ramulus et Uncus could suppress the reactive gliosis, which disturbs the axonal regeneration in the brain of the rat with cerebral infaction after MCAO by controlling the expression of CD81 and GFAP. The effect may be modulated by the up-regulation of c-Fos and ERK. These results suggest that Uncariae Ramulus et Uncus can be a candidate to regenerate CNS injury.

Effects of Induced Microcurrent Shoes on Change of Blood Circulation to patients with Chronic Plantar (미세전류발생 신발 착용이 만성 족저근막염 환자의 혈류량 변화에 미치는 영향)

  • Lee, Yoon-Mi;Choi, Sang-Joon;Cheng, Goh Ah;Cho, Mi-Suk;Cho, Jeong-Sun;Cho, Yong-Ho;Park, So-Hyun;Park, Rae-Joon
    • The Journal of Korean Physical Therapy
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    • v.18 no.3
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    • pp.71-78
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    • 2006
  • Purpose: The purpose of this study was to investigate how induced microcurrent shoes influenced changes of the blood circulation in patients with Plantar Fasciitis. Methods: Initially, the subjects were comprised of 5 males and 5 females, who agreed with this research and are more than fifty years old, but 4 of those were dropped during the experiment. They all have plantar fasciitis and pain on their feet. Subjects wore the induced microcurrent shoes for more than 4 hours everyday during 4 weeks. When they wore those shoes, they also wore the specially produced shocks made of silver-mixed thread and they were asked to avoid intense exercise. Assessments were carried out before and after walking on a treadmill and we measured changes between the test before and after 4 weeks. In the examination of the before test, general shoes were used, and in the examination of the after test, induced microcurrent shoes were used. Temperature difference was measured by thermograpy DOREX spectrum 9000MB(USA). Subjects walked total 20 minutes and during gait, the walking rate on a treadmill was increased from 2Km/h to 3Km/h after 10 minutes. We measured plantar temperature by thermograpy especially both heel, 1st, and 5th metatarsal areas. Results: Firstly, in comparison of the blood circulation on the left and right foot at 0 week and 4 weeks, it tended toward increasing blood circulation but there was. no statistically significant difference (p>0.05). Secondly, in comparison of the blood circulation before and after treadmill with the induced microcurrent shoes, the blood circulation of the heel and the 5th area on the right foot was increased to all subjects(p<0.05). Conclusion: The results of this study revealed that subjects showed tendency to increase blood circulation in both right and left feet after wearing microcurrent shoes and specially after walking treadmill at 4 weeks. Therefore induced microcurrent shoes are useful to improve blood circulation for patients with plantar fasciitis.

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Studies on the Operation Control with Automatic Silk Reeling Process to be responded for Korean Silk Cocoon (한국원료견질에 적응하는 자동조사 공정관리의 구명에 관한 연구)

  • 송기언
    • Journal of Sericultural and Entomological Science
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    • v.17 no.1
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    • pp.1-26
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    • 1975
  • These studies were attempted to find out the optimum silk reeling system by use of automatic silk reeling machine to increase raw silk yield and reeling efficiency with various silk reelable cocoons. The obtained results are as follows; 1. The mean silk reelability ratio(X)of the Korean cocoons during the last ten years was 61 per cent, beside 64.7 per cent in autumn cocoon and 57.3 per cent in spring cocoon. However, the ratio variation of autumn cocoons was larger than that of spring cocoons. 2. A positive correlation between cocoon filament breaks during its process and silk reelability levels was shown to be significant. The cocoons of both poor and good reelability evidenced "J" shape distribution on the filament break graph by the order of reeling cocoon end. Many bave breaks were found at the inner shell of the cocoons, or in case of poor reelability cocoons. 3. The morphology of broken cocoon ends during the process was classified into A, B, C, D, E and F types, The occurrence of B type was majority, but that of F type was minority among them. 4. In case of the cocoon cooking, H-type ion-exchanged soft water was better for good reelability cocoons, Na and H-type ion exchanged neutral soft water for those of fair reelability, and alkaline (Na-type) soft water for those of poor reelability, respectively. 5. The modification of cooking water by mixing the above different types of water (50% Na-type and 50% H-type passed by standard natural water; 75% Na-type and 25% H-type passed by hard natural water; 25% Na-type and 75% H-type passed by soluble natural water) made higher yield of raw silk with tess breaks of thread. 6, In case cocoon ends groping water included sodium hexametaphosphate as much as 800 ppm. the groping efficiency and raw silk yield of cocoon was improved. The effect was pronounced in case of poor reelability cordons. 7. The most reasonable cocoon cooking and silk reeling condition for automatic silk reeling process were observed to be rather incomplete cook with good reelability cocoons and optimum cook with poor reelability cocoons succeeded by the reeling bath temperature of 45$^{\circ}C$, 8. The reasonable silk reeling velocities were observed to be about 150m per min. for good reelability cocoons, 120m per min. for fair reelability ones and 90 to 120m per min. for poor reelability ones. 9. In order to improve the raw silk yield of cocoons and reeling efficiency, the cocoon stand-by-ratio for reeling should be kept at the level of 40 per cent for good reelability cocoons or at 60 per cent for poor reelability ones beside necessary end found cocoon condition.

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Antifouling Activity towards Mussel by Small-Molecule Compounds from a Strain of Vibrio alginolyticus Bacterium Associated with Sea Anemone Haliplanella sp.

  • Wang, Xiang;Huang, Yanqiu;Sheng, Yanqing;Su, Pei;Qiu, Yan;Ke, Caihuan;Feng, Danqing
    • Journal of Microbiology and Biotechnology
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    • v.27 no.3
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    • pp.460-470
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    • 2017
  • Mussels are major fouling organisms causing serious technical and economic problems. In this study, antifouling activity towards mussel was found in three compounds isolated from a marine bacterium associated with the sea anemone Haliplanella sp. This bacterial strain, called PE2, was identified as Vibrio alginolyticus using morphology, biochemical tests, and phylogenetic analysis based on sequences of 16S rRNA and four housekeeping genes (rpoD, gyrB, rctB, and toxR). Three small-molecule compounds (indole, 3-formylindole, and cyclo (Pro-Leu)) were purified from the ethyl acetate extract of V. alginolyticus PE2 using column chromatography techniques. They all significantly inhibited byssal thread production of the green mussel Perna viridis, with $EC_{50}$ values of $24.45{\mu}g/ml$ for indole, $50.07{\mu}g/ml$ for 3-formylindole, and $49.24{\mu}g/ml$ for cyclo (Pro-Leu). Previous research on the antifouling activity of metabolites from marine bacteria towards mussels is scarce. Indole, 3-formylindole and cyclo (Pro-Leu) also exhibited antifouling activity against settlement of the barnacle Balanus albicostatus ($EC_{50}$ values of 8.84, 0.43, and $11.35{\mu}g/ml$, respectively) and the marine bacterium Pseudomonas sp. ($EC_{50}$ values of 42.68, 69.68, and $39.05{\mu}g/ml$, respectively). These results suggested that the three compounds are potentially useful for environmentally friendly mussel control and/or the development of new antifouling additives that are effective against several biofoulers.

A Study on a Real Time Presentation Method for Playing of a Multimedia mail on Internet (인터넷상의 동영상 메일을 재생하기 위한 실시간 연출 기법 연구)

  • Im, Yeong-Hwan;Lee, Seon-Hye
    • The Transactions of the Korea Information Processing Society
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    • v.6 no.4
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    • pp.877-890
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    • 1999
  • In this paper, a multimedia mail including video, sound, graphic data has been proposed as the next generation mail of the text based mail. In order to develop the multimedia mail, the most outstanding problem is the fact that the multimedia data are too huge to send them to the receiving end directly. The fact of big data may cause many problems in both transferring and storing the data of the multimedia mail. Our main idea is to separate between a control program for the multimedia presentation and multimedia data. Since the size of a control program is as small as a plain text mail, it has no problem to send it attached to the internet mail to the receiver directly. Instead, the big multimedia data themselves may remain on the sender's computer or be sent to a designated server so that the data may be transferred to the receiver only when the receiver activates the play of the multimedia mail. In this scheme, our research focus is paced on the buffer management and the thread scheduling for the real time play of the multimedia mail on internet. Another problem is to provide an easy way of editing a multimedia presentation for an ordinary people having no programming knowledge. For the purposed, VIP(Visual Interface Player) has been used and the results or multimedia mail implemented on LAN has been described.

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Study of constitutional difference on man's penis and women's breast (남자 음경(陰莖)과 여자 유방(乳房)의 체질별 크기에 관한 연구)

  • Ryu, Je-hun;Kim, Dal-rai
    • Journal of Sasang Constitutional Medicine
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    • v.11 no.1
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    • pp.281-293
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    • 1999
  • 1. The backdrop of the study. Accurate diagnasis of the constitution is very important Sasang constitution medicine. This study make research of man's penis and woman's breast, make observation these are significant or not in diagnosis of the constitution. 2. The process. The constitution of the people was diagnosed by QSCCII, specialist, (86 persons, men 39, women 47), the men was taken measure of atony and excited length, thickness of the penis with a rule, thread. the women have a personal interview about her height, weight, the girth of the breast, brassiere cup size. 3.The result. This study shows that Tae-eum-in Is 28 persons(32.6%), So-eum-in 37 persons (43%), So-yang-in 21 persons(24.4%). Man's observation is insignificant statistically, woman's observation is insignificant statistically too.

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The approach of properties-flavours theory and the study of morphological standard in ARTEMISIAE HERBA (인진호(茵陳蒿)의 기미론(氣味論)적 해석과 내외부형태연구)

  • Lee, Hwa-Jung;Choi, Mun-Il;Kim, Ja-Young;Kang, Kyoung-Sik;Shin, Chol-Gyun;Jul, Young-Sung;Yun, Ju-Bong
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.19 no.2
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    • pp.130-145
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    • 2006
  • Objectives : The aim of this work is the approaching of properties-flavours theory and the making of morphological standard in Artemisiae herba. Methods : The properties-flavours theory were attempted with bibliographic method and various climate-information. The external-internal morphological standards were determined by using stereoscope and butanol series. Conclusions : The following is a list the conclusion of the approaching of properties-flavours theory and how to make the standard of morphological standard in artemisiae herba. 1. The significant analysis for the artemisiae herba as the properties-flavours theory, is a follow-up survey of the effect written in the official oriental medicine book 2. The other analytic methods according to the gathering time, processing or storing system or especially nature or growth environment(altitude, temperature,. the mean moisture, and the agronomical survey) by way of the explanation of properties-flavours theory appears so many insufficiency at the many cases. 3. In the case of artemisiae herba., there are 2 cases(Artemisia capillaris $T_{HUNB}$ and A. iwayomogi $K_{ITAMURA}$) in current and recently A. aruwa L. is substituted for artemisiae herba in circulation. 4. The external and internal characteristics according to the shape of original plant or herbal states, entirely correspond to all the official oriental medicine boo 5. According to the place of productiot the difference appeals. (1) A. capillatis $T_{HUNB}$. has leaves like a fine thread, and A. iwayomogi $K_{ITAMURA}$ has big and wide leaves, and shows some reddish color, and A. aruwa L. has narrow leaves, its section lines long vertically. (2) In the internal shapes, as the A. capillatis $T_{HUNB}$. leaves', upper epidermal cell wall shows relatively straight form, its all upper- lower epidemical cell wall has a stoma, and calcium rosette crystal is relatively few. As iwayomogi $K_{ITAMURA}$, all upper- lower epidermal cell wall is wavelike concave, and hypodermics has many stoma and many calcium rosette crystal. (3) In the internal shapes of A. aruwa L. the shape of leaves' epidermal cell is irregular and has so many non-glandular hair nad glandular hair on its surface.

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Bending Strength Performance Evaluation of Glass Fiber Cloth Reinforced Cylindrical Laminated Veneer Lumber (직물형 유리섬유로 보강된 원통형 단판적층재의 휨 강도 성능 평가)

  • Lee, In-Hwan;Hong, Soon-Il
    • Journal of the Korean Wood Science and Technology
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    • v.44 no.3
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    • pp.415-423
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    • 2016
  • Cylindrical laminated veneer lumber (LVL) is produced by winding the veneer tape on a circular cylinder. The veneer tape was produced by cutting the veneer into a rectangular shape and sewing it in a vertical direction to the fiber. The tensile strength test was carried out by producing the veneer tape specimen with different species of veneer, types and combinations of sewing yarn. The Radiata pine veneer tape produced with three sewing lines using the reinforced sewing thread had the best tensile strength. Also, the separation and snapping problems of the veneer tape were improved, resulting in the improvement in the workability of cylindrical LVL. The bending strength of various cylindrical LVL produced with different types of veneer tape and a different number of lamination layers and the application of reinforcement with glass fiber cloth was compared with that of Larix log. Bending MOR of cylindrical LVL reinforced with glass fiber cloth at the volume ratio of 11% was improved by 65% in comparison to the non-reinforced cylindrical LVL. In the case of the cylindrical LVL produced with 2 sewing lines of veneer tape, a fracture occurred at the butt joint between the veneer tapes. However, in the case of the cylindrical LVL produced with 3 sewing lines of veneer tape a fracture occurred in the fiber direction.

Changes in Properties of Silk Monofilament Caused by Drawing and Hydrolysis (견 Monofilament의 연신과 가수분해에 의한 특성변화)

  • 김동건;최진협
    • Journal of Sericultural and Entomological Science
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    • v.38 no.2
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    • pp.160-167
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    • 1996
  • The middle silk gland, that is a liquid silk thread gland consisting of silk protein, was taken out and a silk monofilament was made by drawing rapidly to approximately 3 times. In order to deteriorate the inter molecular hydrogen bonding force and to stretch in, the drawn silk filament was swoolen in boiling water. The results obtained are as follows ; The silk gland sample that just dried silk gland was occupied in crystalline region of silk-I type and random amorphous region. According to the examination of X-ray diffraction and thermal analysis, silk-II type crystal begins to appear partially in monofilament sample and spread to almost complet silk-II type crystal in 65.2% drawn sample. And, orientation of silk fibroin mlecule increased suddenly in early stage with a rise of drawing ratiofrom birefringence and density, and it was found that orientation of fibroin molecule was completed. As drawing ratio increases relation with time of hydrolysis, birefringence appeared almost fixed a tendency. Crystallization collapse by hydrolysis was not found in X-ray diffraction and thermal analysis. But, amorphous region began to flow by treated hydrolysis, that orientation of crystallization part was disturbed was supposed.

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