• Title/Summary/Keyword: Thiobacillus sp. IW

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Removal of Hydrogen Sulfide by Biofilter Media (담체 종류에 따른 황화수소의 제거특성)

  • Cha, Gyusuk
    • Journal of the Korean GEO-environmental Society
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    • v.8 no.4
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    • pp.41-45
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    • 2007
  • It is very important that selection of packing media with large surface area, high limited back pressure in biofilter. The object of this study is the isolation of sulfur-oxidizing bacteria and the removal of hydrogen sulfide in biofilter by media. This investigation led to the following results: 1) we isolated Thiobacillus sp. IW. at an abandoned coal mine in Hwasun, Jeonnam Province. 2) The inorganic media showed better results than the organic media from experiments looking at removal characteristics and changes in pressure drop using various media. 3) Among the inorganic media, fibril and PU media showed best performance.

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Removal of Hydrogen Sulfide and Methylmercaptan Using Thiobacillus in a Three Phase Fluidized Bed Bioreactor

  • KIM, KYUNG-RAN;KWANG-JOONG OH;KYUNG-YONG PARK;DONGUK KIM
    • Journal of Microbiology and Biotechnology
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    • v.9 no.3
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    • pp.265-270
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    • 1999
  • A three phase fluidized bed bioreactor immobilized with Thiobacillus sp. IW was tested to remove hydrogen sulfide and methylmercaptan with high loading rate. In a single gas treatment, the bioreactor removed 92- 98% of hydrogen sulfide with loading rate of 15- 66 g/l/h and removed 87-98% of methylmercaptan with loading rate of 14-60 gl/sup -1/h/sup -1/. In the mixed gas treatment, the removal efficiencies of hydrogen sulfide and methylmercaptan maintained at 89-99% for various inlet loading rates and were not affected by the inlet loading ratio of both gases in low loading rates. When the inlet concentration of methylmercaptan increased 3.8 times and was maintained for 30 h to observe the response of the bioreactor to sudden environmental change, the removal efficiency of methylmercaptan was maintained at an average of 91%.

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Removal of Hydrogen Sulfide, Ammonia, and Benzene by Fluidized Bed Reactor and Biofilter

  • Kim, Chong-Woo;Park, Jin-Su;Cho, Sung-Ki;Oh, Kwang-Joong;Kim, Young-Sik;Kim, Dong-Uk
    • Journal of Microbiology and Biotechnology
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    • v.13 no.2
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    • pp.301-304
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    • 2003
  • In this study, hydrogen sulfide ($H_2S$), ammonia ($NH_3$), and benzene, which represent the major odor from a natural leather process plant, were removed using a fluidized bed bioreactor and biofilter including Thiobacillus sp. IW and a MY microbial consortium. The critical removal rate was $12g m^{-3}h^{-1}\;for\;H_2S,\;11g m^{-3}h^{-1}\;for\;NH_3\;and\;28 g m^{-3}h^{-1}$ for benzene by the fluidized bed bioreactor, and $8.5g m^{-3}h^{-1}\;for\;H_2S\;7g m^{-3}h^{-1}\;for\;NH_3,\;and\;25 g m^{-3}h^{-1}$ for benzene in the biofilter. The average removal efficiency of $H_2S$, $NH_3$, and benzene by continuous operation for over 30 days with the fluidized bed bioreactor was $95{\pm}3\%,\;99{\pm}1\%,\;and\;98{\pm}5\%$, respectively, whereas that with the biofilter was $96{\pm}4\%,\;95{\pm}4\%,\;and\;97{\pm}3\%$, respectively. Therefore, the critical removal rate of $H_2S$, $NH_3$, and benzene was higher in the fluidized bed bioreactor, whereas the removal efficiency on the continuous operation was similar in both bioreactors.

Polyurethane을 이용한 Thiobacillus sp. IW의 고정화

  • Hwang, Eun-Sang;Im, Geun-Gil;Lee, Gwang-U;Gang, Chun-Hyeong;Ryu, Hwa-Won;Park, Don-Hui
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.536-539
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    • 2000
  • A simple and effective method has been developed for the immobilization of the cell on polyurethane foam. Two types of commercially available polyurethane foam and Hydro-filt were tested. The ultimate purpose of the process is to produce low-cost materials for hydrogen sulfide removal which are being increasingly used for industrial application. Effect of several parameters were studied on the cell loading. These parameters were type, size, and amount of polyurethane foam. MC-70 was the best immobilization material of three type of carriers, and optical particle size was $5{\sim}8mm$ and amount of polyurethane foam was 8g/L.

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Characteristics on the Incubation of Sulfur Compound-Oxidizing Strains Separated for the Removal of Malodor (악취제거를 위하여 분리한 황화합물 산화균주의 배양특성)

  • Lim, Dong Joon;Lim, Kwang-Hee
    • Korean Chemical Engineering Research
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    • v.47 no.6
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    • pp.788-794
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    • 2009
  • Both strains of KD-1212 and DAH-1056 were isolated and identified from animal manure-contaminated soil by screening bacterial strains for the removal of sulfur compound-malodor with such substrate as sodium thiosulfate or free sulfur. Then the characteristics on the incubation of these microbes were observed under various incubating-condition such as pH, temperature, aerobic or anaerobic, substrate(sulfur compound) concentration, nitrogen and carbon source and rotating speed for mixing, and the optimum incubating condition was established. The optimum pHs of KD-1212 and DAH-1056 were 7.0 and 4.0, respectively, and their optimum temperatures were in the range of $30{\sim}35^{\circ}C$. Another autotrophic strain, ED-1138, was isolated from contaminated soil. The strain DAH-1056 excelled a strain Thiobacillus sp. IW in eliminating hydrogen sulfide during the process of malodor-biofiltration with a fixed strain. The characteristics on the incubation of strain KD-1212 were observed under various substrate-concentrations, nitrogen and carbon sources. KD-1212 favored glucose and maltose, and yeast extract as carbon sources and nitrogen source, respectively. The optimum concentrations of substrate and nitrogen source were 25 mM of sodium thiosulfate and 0.5% yeast extract, respectively for the growth of strain KD-1212.

Semi-pilot Scaled Hybrid Process Treatment of Malodorous Waste Air: Performance of Hybrid System Composed of Biofilter Packed with Media Inoculated with Thiobacillus sp. IW and Return-sludge and Photocatalytic Reactor (악취폐가스의 세미파일럿 규모 하이브리드 공정 처리: Thiobacillus sp. IW 및 반송슬러지를 접종한 담체를 충전한 바이오필터와 광촉매반응기로 구성된 하이브리드시스템의 운전)

  • Lee, Eun Ju;Lim, Kwang-Hee
    • Korean Chemical Engineering Research
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    • v.52 no.2
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    • pp.191-198
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    • 2014
  • A semi-pilot hybrid system composed of a photocatalytic reactor and a biofilter was operated under various operating conditions in order to treat malodorous waste air containing both hydrogen sulfide and ammonia which are major air pollutants emitted from composting factories and many publicly owned treatment works (POTW). When both hydrogen sulfide and ammonia contained in malodorous waste air were treated simultaneously by a biofilter system, its performance of ammonia removal was much more poor than that by a biofilter system treating waste air containing only ammonia, unlike its performance of hydrogen sulfide removal. For semi-pilot hybrid system, the removal efficiencies of hydrogen sulfide and ammonia turned out to be ca. 83 and 65%, respectively. Therefore, for semi-pilot hybrid system, the removal efficiencies of hydrogen sulfide and ammonia was increased by ca. 4 and 30%, respectively, compared to those of semi-pilot biofilter system (control). In addition, the maximum elimination capacities of hydrogen sulfide and ammonia for semi-pilot hybrid system turned out to be ca. 60 and $37g/m^3/h$, respectively. These maximum elimination capacities of hydrogen sulfide and ammonia were estimated to be ca. 9.1% and ca. 23.3% greater than those for semi-pilot biofilter system (control), respectively. Therefore, the semi-pilot hybrid system contributed the enhancement of removal efficiency and the maximum elimination capacity of ammonia in a higher degree than that of hydrogen sulfide, compared to the semi-pilot biofilter system.

Utilization of biofilter/photo-catalytic reactor system for the simultaneous treatment of hydrogen sulfide and toluene from waste-air

  • Lim, Kwang-Hee;Ping, Wang Zhi;Lim, Dong-Joon;Lee, Eun-Ju
    • 한국생물공학회:학술대회논문집
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    • 2005.04a
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    • pp.287-292
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    • 2005
  • Transient behavior of biofilter/photo-catalytic reactor system was observed to eliminate both hydrogen sulfide and toluene from waste air at its four sampling ports. The biofilter was packed with a equivolume mixture of granular activated carbon(GAC) and compost as packing media on which Thiobacillus sp. IW and Burkholderia cepacia G4 were inoculated and were fixed. The biofilter/photo-catalytic reactor system was run for eight stages of operation under various operating conditions. As a result the removal efficiencies of hydrogen sulfide and toluene began to decrease from 100% after the inlet loads of hydrogen sulfide and toluene surpassed ca. 100 $S-g/m^{3}/h$ and $161g/m^{3}/h$, respectively, and were rapidly decreased to 60% after the inlet loads of hydrogen sulfide and toluene were increased to 200 $S-g/m^{3}/h$ and $644g/m^{3}/h$, respectively.

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