• KSBB Journal
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• v.11 no.6
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• pp.649-653
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• 1996

Imnmobilization characteristics of Thiobacillus sp. IW which oxidizes sulfur compound was studied to use the bacterium in odor controlling equipment for the future. The optimum growth conditions for Thiobacillus sp. IW were pH7, $30^{\circ}C$ and the generation time was 38min, which was extremely fast compared with other sulfur oxidizing bacteria. Optimum growth conditions in activated carbon as a carrier was pH5, $35^{\circ}C$ and those in bioceramics was pH 7∼8, $35^{\circ}C$. Cell growth immobilized in bioceramics was more stable in pH, temperature change than that immobilized in activated carbon and total number of cells in bioceramics were also higher. Based on these results, the bioceramics is thought to be better carrier in immobilization of Thiobacillus sp. IW.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.1
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• pp.48-52
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• 2000

An autotrophic sulfur oxidizer, Thiobacillus sp. ASWW-2, was isolated from activated sludge, and its sulfur oxidation activity was characterized. Thiobacillus sp. ASWW-2 could oxidize elemental sulfur on the broad range from pH 2 to 8. When 5-50 g/L of elemental sulfur was supplemented as a substrate, the growth and sulfur oxidation activity of Thiobacillus sp. ASWW-2 was not inhibited. The specific sulfur oxidation rate of strain ASWW-2 decreased gradually until sulfate was accumulated in medium up to 10 g/L. In the range of sulfate concentration from 10 g/L to 50 g/L, the sulfur oxidation rate could keep over $2.0g-S/g-DCW{\cdot}d$. It indicated that Thiobacillus sp. ASWW-2 has tolerance to high concentration of sulfate.

• Korean Journal of Microbiology
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• v.32 no.4
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• pp.252-257
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• 1994

A new hydrogen sulfide-oxidation bacterium, Thiobacillus sp. was isolated from waste coal mine water around Hawsun in Chunnam province. The isolate was motile gram-negative rod shape, formed spore and grew up to be aerobically facultative chemolithotroph by using energy released from the oxidation of reduced inorganic sulfur compounds. It could assimilate various kinds of organic compounds and grew well upon thiosulfate-supplemented basal medium. To the lelvel of 32 mM in thiosulfate concentration, thiosulfate in itself was utilized as energy source for growth. However, from those of the higher concentration than 32 mM, thiosulfate functioned specifically as the substrate inhibitor rather than as the energy source. It was found that the optimum thiosulfate concentration for growth was 32 mM. The G+C content of the DNA was 65.0 mol%. The isolate had 16 : 1 + 17$_{cyc}$, 16 : 0 as their major non-hydroxylated cellular fatty acids, 3-OH 12 : 0 as a hydroxylated fatty acid and also contained unidentified $C_{18}$ branched fatty acid. The ubiquinone system in the respiratory chain was Q-9. Based on the physiological and biochemical characteristics, the isolate was assigned to a novel species of the genus Thiobacillus sp. iw.

• Korean Journal of Microbiology
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• v.40 no.2
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• pp.127-132
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• 2004

We isolated 50 strains from sludge of wastewater treatment aeration tank using selective medium for Thio-bacillus sp. by membrane filtration method. They were identified as Thiobacillus neapolitanus (7), T. tepidarius (4), T. dientrificans (5), T. versutus (23), T. intermedius (2) and T. perometabolis (9). We selected Thiobacillus versutus strain KT51, which had the highest removal efficiency (100％) of hydrogen sulfide and the highest removal efficiency (85％) of dimethyl sulfide for 30 min in screen test. Also Thiobacillus versutus strain KT81 had the highest removal efficiency (26％) of dimethyl disulfide for 30 min in screen test. In applification of lab-scale reactor (closed-biological treatment) using Thiobacillus versutus strain KT51, results were 99.8％ (＜0.02 ppm) removal efficiency of hydrogen sulfide for 15 min.

• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.419-422
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• 2000

A three-phase fluidized-bed bioreactor including Thiobacillus sp. IW was tested to remove H_2S and $NH_3$ simultaneously. The inlet $H_2S$ was oxidized to $SO_4^{2-}$ by Thiobacillus sp. IW, and the $NH_3$ reacted with the $SO_4^{2-}$ to form $(NH_4)_2SO_4$. The removal efficiency of $H_2S$ was 98.4-99.9% for an inlet concentration of 36-730 ppm and that of $NH_3$ was 60.2-99.2% for an inlet concentration of 45-412 ppm. The removal efficiency of $NH_3$ was reduced when the inlet loading rate of $NH_3$ was increased above 10 mg/l/h. When the bioreactor was operated for 25 days with a lower inlet concentration of $NH_3$ compared with the of $H_2S$, the bioreactor exhibited an excellent performance with a stable pH, dissolved oxygen content, and cell concentration.

• Korean Journal of Environmental Agriculture
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• v.25 no.2
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• pp.124-128
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• 2006

To reducing offensive odor form compost UIW-6 mutant obtained by UV treatment from sulfur-oxidizing bacteria, Thiobacillus sp. IW. The UIW-6 mutant was found 1.6 times faster in growth than the parent strain on thiosulfate medium (TM) at 36 h after incubation. Initial pH, temperature and agitation for the optimum growth of UIW-6 were 6.5, $35^{\circ}C$ and 200 rpm, respectively. The UIW-6 mutant growth was two times higher than parent strain at 6 h culture in TM liquid medium containing 50 mM sodium thiosulfate. The UIW-6 mutant used fructose and sucrose as carbon sources and yeast extract> tryptone> peptone as nitrogen ones. It was found that the growth of UIW-6 was increased in addition of 0.2% yeast extract.

• KSBB Journal
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• v.9 no.3
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• pp.287-293
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• 1994

A bacterium isolated from waste coal mine water around Hawsun had an ability for the degradation of hydrogen sulfide. The isolate was identified as Thiobacillus sp. IW. on the basis of its morphological, physiological and chemotaxonomical characteristics. The optimum pH and temperature were 7 and $30^{\circ}C$, respectively. Growth occurred in a pH range of 3 to 9. Due to the sulfate accumulated in liquid medium, the pH decreased. As a consequence the cell growth was inhibited. Potasium nitrate and glutamic acid were utilized as a nitrogen source but urea and ammonium chloride not consumed. Denitrification occurred in a basal medium containing the glucose but did not in a basal medium containing the malate. The maximum specific growth rate of cell was 0.78h-1 and generation time was 0.9 hour. The cell productivity was 6.25mg/1$.$h and the isolate grew logarithmically up to 18 hour. These results indicate that the isolate can be a suitable bacterium responsible for degradation of hydrogen sulfide as malodorous compounds.

• Journal of Microbiology and Biotechnology
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• v.9 no.3
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• pp.265-270
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• 1999

A three phase fluidized bed bioreactor immobilized with Thiobacillus sp. IW was tested to remove hydrogen sulfide and methylmercaptan with high loading rate. In a single gas treatment, the bioreactor removed 92- 98％ of hydrogen sulfide with loading rate of 15- 66 g/l/h and removed 87-98％ of methylmercaptan with loading rate of 14-60 gl/sup -1/h/sup -1/. In the mixed gas treatment, the removal efficiencies of hydrogen sulfide and methylmercaptan maintained at 89-99％ for various inlet loading rates and were not affected by the inlet loading ratio of both gases in low loading rates. When the inlet concentration of methylmercaptan increased 3.8 times and was maintained for 30 h to observe the response of the bioreactor to sudden environmental change, the removal efficiency of methylmercaptan was maintained at an average of 91％.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.339-342
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• 2000

A three phase fluidized bed bioreactor including Thiobacillus sp.IW was used to remove hydrogen sulfide and ammonia simultaneously. In this study, hydrogen sulfide was oxidized to sulfate by the microorganism and ammonia was reacted with the sulfate to form ammonium sulfate. Removal efficiency of hydrogen sulfide was almost perfect up to 45 mg/l h of inlet loading rate, whereas that of ammonia was reduced as inlet loading rate increased from 10 mg/1 h.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.163-166
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• 2000

Hydrogen sulfide, ammonia and benzene which are generated from natural leather industry were simultaneously removed using biofilter including benzene degrading microbial consortia and sulfur oxidizer Thiobacillus sp.IW. The removal efficiency of benzene was maintained 90% in average for single and mixed gas treatment and that of ammonia was 99%, whereas at of hydrogen sulfide was relatively lower 85%.