• Preventive Nutrition and Food Science
• /
• 제1권2호
• /
• pp.203-207
• /
• 1996

Methanol extract of dried persimmon leaves was refractionated using hexane, chloroform, ethylacetate, n-butanol aqueous fractions. Among these chloroform fraction showed the highest inhibition rate on the mutagenicities of aflatoxin B₁(AFB₁) and 3,2' -dimethyl-4-amino-bipheny1(DMAB) in Salmonella typhimurium TA 100. Chloroform fraction was further fractionated into eight fractions by silica gel column chromatography and thin layer chromatography(TLC). The fraction 5 on TLC exhibited the highest antimutagenic activities in AFB₁and DMAB. 2,4-Decadienal, dihydro-4-methyl-2(3H)-furanone, hexanoic acid 1,4-bis(1-methy1 ethy1)benzene, heptanoic acid, phenol, octanoic acid, nonanoic acid and benzoic acid were tentatively identified from this antimutagenic fraction by GC-MS.

• 한국응용과학기술학회지
• /
• 제15권3호
• /
• pp.27-31
• /
• 1998

Antioxodative substances in Mulberry leaves were examined. Antioxidative substances in Mulberry leaves were extracted by 80% methanol agueous solution. Antioxidative activity of extract was determined by examining hydrogen donating ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the inhibitory effect on the formation of the peroxide from Linoleic acid in the test tube at $50^{\circ}C$. Antioxidative substance were, then, separated and indentified by thin layer chromatography(TLC), UV-Vis spectrum and High performance liquid chromatography(HPLC) methods. Hydrogen donating ability on DPPH and antioxidative ability on linoleic acid of the extracted antioxidative substance were higher than those of 100ppm butylated hydroxy toluene(BHT). The extracted antioxidative substances were separated by TLC using ethylacetate : chloroform : formic acid : water(8 : 1 : 1 : 1 v/v) as a solvent, and a spot at Rf=0.35 was detected. The spot was scraped from the plate, and extrated by methanol. The extract was analyzed by UV-Vis spetra and HPLC, and chlorogenic acid was identified as a antioxidative substance.

• 한국미생물·생명공학회지
• /
• 제27권3호
• /
• pp.179-183
• /
• 1999

The volatile components (essential oil) showing antimicrobial activity were extracted from the fruit peel of Zanthoxylum piperitum DC by distillation and separated by thin layer chromatography (TLC). The crude volatile components exhibited antimicrobial activity only at very high concentration. The active fraction obtained by TLC inhibited noticeably the growth of bacteria. The minimum inhibitory concentration (MIC) of the fraction were 150ppm, 300ppm, and 300ppm against Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis, respectively. The components in the active fraction were identified by gas chromatography/mass spectrometry to be geranlyl acetate (60.23%), citronellal(36.01%), citronellol(3.77%), geraniol(0.46%), and cumin ldehyde(0.43%).

• Archives of Pharmacal Research
• /
• 제9권1호
• /
• pp.5-9
• /
• 1986

Partialy purified ginseng proteins were either treated with sodium dodecyl sulfate (SDS) and .betha.-mercaptoethanol to denature the proteins or not, and subjected to Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) to compare the components of each fraction. Standard proteins of known molecular weights (MW) were also either treated with SDS and .betha.-mercaptoethanol or not, and subjected to HPLC to obtain regression lines for MW determination. From the retention times obtained from samples in eiether case by HPLC, the MW were estimated as following in SDS treated condition, Gl fraction showed three peaks each with MW of above 100, 000, 51, 000 and 19, 000. Gll showed one original peak with MW of 21, 000 and Gll, two peaks each with MW of 19, 000 and 14, 000. On the other hand, in non-SDS treated condition, GI fraction showed two peaks each with MW of above 200, 000 NA 52, 000. Gll showed one original peak with MW of 41, 000 and Gill, three peaks each with MW of 28, 000, 19, 000 and 14, 000.

• 한국식품영양과학회지
• /
• 제6권1호
• /
• pp.66-71
• /
• 1977

An investigation of certain tar food colorants required a relatively inexpensive TLC adsorbent to be used in preparative thin-layer chromatography procedure. As will be shown activated clay and Kieselguhr has excellent physical characteristics for T LC applications, is easily applied, and is good for analytical work as well as showing good separator capabilities with selected groups of compounds. In addition to its usefulness its economical price suggested this article.

• 대한화학회지
• /
• 제47권4호
• /
• pp.401-411
• /
• 2003

제7차 교육과정의 가장 큰 특징은 수준별 교육과정의 시행이라고 할 수 있으며, 이에 따라 학생의 능력과 개인차에 따른 다양한 학습 과제의 개발이 필요하다. 제6차 교육과정의 중학교 과정에서 다루었던 크로마토그래피의 이론과 실험의 오류를 바로잡기위하여 중학생들이 크로마토그래피의 원리를 보다 정확하게 이해할 수 있는 새로운 실험 키트가 필요하다. 본 연구에서는 시중에 판매되고 있는 TLC판을 정지상으로 이용하고, 이동상으로는 상대적으로 인체에 해가 적은 혼합 유기용매를 사용하며, 시료 및 참고물질로는 시료의 전개 과정을 눈으로 쉽게 확인할 수 있는 산-염기 지시약들을 사용하여 새로운 크로마토그래피 실험 키트를 개발하였다. 또한, 이 실험 키트의 효과를 알아보기 위하여 중학생(일반 중학생 및 영재반 중학생)과 교사(과학 교사 및 비과학 교사)를 상대로 실험을 실시하여, 실험내용과 그 효과에 관한 설문을 조사하였다.

• 생약학회지
• /
• 제41권2호
• /
• pp.136-140
• /
• 2010

This study was carried out to utilize non-toxic solvents instead of harmful solvents for the TLC(Thin Layer Chromatography) identification test of herbal medicines. It is recommended not to use harmful solvent such as chloroform at a viewpoint of clean analysis. In this study, we revised the identification test of 10 items in the Korea Pharmacopoeia(KP) and the Korean Herbal Pharmacopoeia(KHP) such as Cornus officinalis S. containing the harmful solvents on the developing solvent and established identification test that is utilized non-toxic solvents by HPTLC(High Performance Thin Layer Chromatography).

• 한국식품과학회지
• /
• 제32권2호
• /
• pp.439-443
• /
• 2000

시설원예산물 중 DON 생성능이 있는 균주의 오염 여부를 조사하기 위해 진주를 비롯한 서부 경남 일원과 경북 안동 근교에서 총 192점의 시료를 분리하여 균 분리를 실시한 결과 Fusarium속 103균주를 분리하였다. 분리된 균을 YES배지로 $28^{\circ}C$, 15일 배양한 후 ethyl acetate로 추출하여 thin layer chromatography(TLC)법으로 확인한 결과 DON 생성 균주 8개가 확인되었다. Enzyme amplification system immunoassay법을 이용하여 DON 생성 균주 8개를 정량한 결과 검출범위는 $0.007-1.21\;{\mu}g/mL$ 이었다. 이 중 남해 토양에서 분리한 32-D-3 균주가 $1.21\;{\mu}g/mL$로 가장 높았다.

• 한국산업보건학회지
• /
• 제6권2호
• /
• pp.202-208
• /
• 1996

The purposes of this study are the identification and determination of metabolites in the isolated rat liver perfusate of carbon disulfide by two-dimentional thin-layer chromatography and high performance liquid chromatography for understanding the metabolism of carbon disulfide. 2-Thio-thiazolidine-4-carboxylic acid(TTCA) was synthesized by the reaction of carbon disulfide and cysteine, and confirmed by two-dimentional thin-layer chromatography, high performance liquid chromatography, UV spectroscopy, and IR spectroscopy. The absorbance of UV detector for the simultaneous determination of TTCA and thiocarbamide was 254 nm although their maximum spectra were 273 nm and 237 nm, respectively. Two kinds of the developing solvent in the two-dimentional thin-layer chromatography were 2-butanol : 80% HCOOH : $H_2O$ (7 : 2 : 1) as the first developing solvent and 2-propanol : $H_2O$ (4 : 1) as the second developing solvent. After perfusion of carbon disulfide ($8274.23{\mu}mol$), the amount of TTCA and thiocabamide of the perfusate(100 ul) were $12.02-16.4{\mu}mol$ and $5.25-8.15{\mu}g$, respectively. The mean amount of them were $14.08{\mu}mol$ and $6.41{\mu}mol$ respectively, and the former was 2.20 times greater than the latter. For conforming the mechanism of formations of TTCA and thiocarbamide in vivo, we have to clarify whether the reactions between carbon disulfide and ammonia, ammonium salts, amides, cysteine, cystine, or proteins will be formed in vitro.

• 한국환경보건학회지
• /
• 제27권4호
• /
• pp.35-40
• /
• 2001

In order to evaluate the safety of agricultural products in Korea, we carried out work by screening of Fusarium species. which can produce deoxinivalenol(DON) from agricultural products in Western Gyeongnam, Korea. From 215 samples of soil agricultural products, 129 strains of Fusarium species were obtained. The production of DON was verified by thin layer chromatography(TLC) As the results of TLC, 25 strains were identified as DON producing strain. But only 10 strains were identified as DON producing strains by enzyme-linked immunosorbent assay (ELISA). The maximum DON producing strain No.41 was isolated from corn. In conclusion. the above results indicate that DON producing fungi contaminated agricultural products in Korea. Therefore further studies are required to accumulate more detailed data about the contamination of DON in various agricultural products.