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Effects of Oral Intake of Kimchi-Derived Lactobacillus plantarum K8 Lysates on Skin Moisturizing

  • Kim, Hangeun;Kim, Hye Rim;Jeong, Bong jun;Lee, Seung Su;Kim, Tae-Rahk;Jeong, Ji Hye;Lee, Miyeong;Lee, Sinai;Lee, Jong Suk;Chung, Dae Kyun
    • Journal of Microbiology and Biotechnology
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    • v.25 no.1
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    • pp.74-80
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    • 2015
  • Skin is the soft outer covering of vertebrates that provides protection from pathogenic infection, physical damage, or UV irradiation, and controls body temperature and water content. In this study, we examined the effects of oral intake of kimchi-derived Lactobacillus plantarum K8 lysates on skin moisturizing. In an in vitro study, we observed that the hyaluronic acid content increased in HaCaT cells treated with L. plantarum K8 lysates. Oral administration of L. plantarum K8 lysates effectively attenuated the horny layer formation and decreased epidermal thickening in DNCB-treated SKH-1 hairless mice skin. The damage to barrier function was reduced after 8 weeks of oral administration of L. plantarum K8 lysates as compared with that in the atopic dermatitis mice. For the test with volunteers, we manufactured experimental candy containing 2.1% L. plantarum K8 lysates, while control candy did not contain bacterial lysate. A significant increase in hydration in the experimental candy-administered group as compared with the control candy-administered group was observed on the face after 4 and 8 weeks, and on the forearm after 4 weeks. Decreases in horny layer thickness and TEWL value were observed on the face and forearm of the experimental group. Together, the in vitro cell line and in vivo mouse studies revealed that L. plantarum K8 lysates have a moisturizing effect. A clinical research study with healthy volunteers also showed an improvement in barrier repair and function when volunteers took L. plantarum K8 lysates-containing candy. Thus, our results suggest that L. plantarum K8 lysates may help to improve skin barrier function.

The Development of the Conceptual Model Visualization Program (개념모델 가시화 프로그램의 개발)

  • Choi, Hong-Seok;Okazaki, Akira
    • Science of Emotion and Sensibility
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    • v.13 no.3
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    • pp.573-580
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    • 2010
  • The earlier KANSEI evaluation had to choose not to overlap the opposite meaning of the words, had to choose that some figures are shown step by step and had to choose be prepared things in advance. However, The Study shows a method which visualizes the imprecise evaluations of human without choosing evaluation items which are not prepared figures. The program is used the way which is entered by hand-drawing that the subjects use the way to enter their subjective feelings about the evaluations presented by using the mouse or the graphics tablet. It is possible to express imprecise things about evaluations and the method of drawing is free. The program provides that we can express our thinking by drawing and there are a few limits and the prescribed standard. The area ratio of each circle is expressed as a pie chart and the area of circle what is drawn which is counted automatically at the same time. These things are possible not only to modify a transparency, a thickness of a line, a color and the area of circle what is drawn, but also to adjust the area of circle. The conceptual model is visualized that expresses something by hand-drawing such as a circle. The conceptual model has wide range of applications such as a personnel evaluation, a suffering evaluation and product evaluation. The program currently has been testing the effective of the program's possibilities which is used with the personnel appraisal tool of the nurses themselves who work in nurse support department where the St. Marianna University School of Medicine Hospital is. And also the program is proceeding with development to visualize the conceptual model by dynamic interfaces effectively and the program is applied such as KJ method and a program is used to express the kind of the patient's pain and its level.

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Effect of Groove Conditions on the Mechanical Properties of Welds Produced by the Combined Welding Process of Flux Cored Arc and Electro Gas in EH36 TMCP Steel Plate for Hull Structures (선체구조용 EH36 TMCP 후판의 FCAW 및 EGW 조합 용접부 기계적 성질에 미치는 개선조건의 영향)

  • Kim, Kihyuk;Kim, Kiwon;Sim, Hoseop;Bae, Kangho;Hong, Hyunuk;Park, Byunggyu
    • Journal of Welding and Joining
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    • v.33 no.5
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    • pp.35-40
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    • 2015
  • Characterization of microstructures and mechanical properties of 83mm thickness EH36-TM welds produced by the combined flux cored arc (FCA) and electro gas (EG) welding processes has been studied with the two different groove conditions, single-V (SV) and double-V (DV) bevels. The welding consumables used for FCA and EG welding processes were ASME/AWS A5.29 (E81T1-K2) and A5.26 (EG72T), respectively. Experimental results showed that all the mechanical properties of welds such as tensile property, CVN toughness and Vickers hardness met IACS requirements. The tensile strength of EG welded plates were reduced by approximately 4% (DV: 3.8%, SV: 4.2%) compared to the base metal. The hardness value of SV-beveled weld metal was slightly lower than that of DV-beveled one. There were no significant differences as per welding groove conditions except for the weld metal. In addition, at the fusion line, the toughness of SV condition was 20J lower and the weld metal was 40J lower than DV condition, respectively. On the basis of microstructural analysis, grain boundary ferrite (GBF) structures for SV condition were 2 times higher volume fraction than for DV condition and their packet sizes were coarsened to almost double. It was thus suggested that the GBF volume fractions and packet sizes in the weld metal of EH36-TM steel plates are the most important factors affecting the mechanical properties of the combined FCA and EG welded joint. Nevertheless, all the results of welds with both DV and SV conditions were found to be excellent.

Development of Two Dimensional Blade Section with High Efficiency for Marine Propeller (선박 프로펠러용 고효율 2차원 날개단면 개발)

  • Na, Yun-Cheol;Song, In-Haeng;Ahn, Jong-Woo
    • Journal of the Society of Naval Architects of Korea
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    • v.34 no.1
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    • pp.11-23
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    • 1997
  • This paper contains a new approach to blade section design method for marine propellers. The hydrodynamic characteristics of 2-D section are highly influenced by its geometrical parameters i.e., thickness and camber distributions and leading edge radius etc. To consider fully turbulent flow field near 2-D section. the finite volume method with k-${\varepsilon}$ turbulent model which solve Reynolds time averaged Navier-Stokes(RANS) equation is applied. In this study, O-type grid system that can provide many calculation points on blade surface is used. The results were compared with those of the experiment of NACA0012 to confirm the accuracy of the developed codes. The goal of this study is the development of a blade section with high efficiency and low drag. To achieve this, we carried out the tests of lift, drag and cavitation characteristics in cavitation tunnel. The results of experiment were compared with numerical results in order to validate the proposed blades design method. By comparing the numerical results with the experiments, we found that the new blade section, KH28 allows superior performance in efficiency and cavitation avoidance characteristics. We further investigated the blade section design method and an application study of this section, KH28 to apply to the marine propeller. In order to improve the accuracy of numerical results on prediction of lift and drag, we conclude here that the 2-layer boundary model must be used.

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Damage Evaluation of Track Components for Sleeper Floating Track System in Urban Transit (도시철도 침목플로팅궤도 궤도구성품의 손상평가)

  • Choi, Jung-Youl;Kim, Hak-Seon;Han, Kyung-Sung;Jang, Cheol-Ju;Chung, Jee-Seung
    • The Journal of the Convergence on Culture Technology
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    • v.5 no.4
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    • pp.387-394
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    • 2019
  • In this study, in order to evaluate the damage and deterioration of the track components of sleeper floating track (STEDEF), the field samples(specimens) were taken from the serviced line over 20 years old, and the track components were visually inspected, and investigated by laboratory tests and finite element analysis. As a result of visual inspection, the damage of the rail pad and fastener was slight, but the rubber boot was worn and torn at the edges of bottom. The resilience pads were clearly examined for thickness reduction and fatigue hardening layer. As a result of spring stiffness test of rail pad and resilience pad, the deterioration of rail pad was insignificant, but the deterioration of resilience pad exceeded design standard value. Therefore resilience pad was directly affected by train passing tonnage. As a result of comparing the deterioration state of the field sample and the numerical analysis result, the stress and displacement concentration position of the finite element model and the damage position of the field sample were coincident.

Comparative Effects of Phytase Derived from Escherichia coli and Aspergillus niger in Sixty Eight-week-old Laying Hens Fed Corn-soy Diet

  • Yan, L.;Zhou, T.X.;Jang, H.D.;Hyun, Y.;Kim, H.S.;Kim, I.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.10
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    • pp.1391-1399
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    • 2009
  • Two hundred and sixteen 68-week-old Hy-Line brown laying hens were used in a 6-week feeding trial to compare the efficacy of phytases Optiphos (OPT) and Natuphos (NAT), which were isolated from Escherichia coli and Aspergillus niger, respectively. Hens were randomly allotted into six treatments with six replications (six layers in three adjacent cages) per treatment according to their initial BW. The hens were then subjected to one of the following dietary treatments: i) Positive Control (PC; available phosphorus (AP) 0.4%); ii) Negative control (NC; AP 0.2%); iii) NAT1 (NC+250 FTU/kg NAT); iv) NAT2 (NC+500 FTU/kg NAT); v) OPT1 (NC+250 FTU/kg OPT); vi) OPT2 (NC+500 FTU/kg OPT). Feed intake, egg production, egg quality, apparent nutrient digestibility and serum P and Ca concentration were evaluated to compare the effect of the two phytases. Feed intake and eggshell thickness were not affected by the treatments. Superior effects (p<0.05) of OPT were only observed in egg production and egg weight compared with NAT. Characteristics such as eggshell breaking strength, apparent digestibility of N, Ca and P and serum P concentration were equally increased with the supplementation of both phytases (p<0.05), where no significant difference was observed in those characteristics between PC and phytase supplementation at 500 FTU/kg. Equally effective improvements (p<0.05) were also observed in egg production and DM digestibility, where no improvements were observed (p<0.05) between the PC group and the groups with phytase supplementation at 500 FTU/kg. Equal increases in the serum Ca level were observed when the groups with phytase supplementation were compared to the PC group. Overall, the results of this study suggest that NAT and OPT are equally effective at liberating phytate-bound complexes when included in 0.2% available phosphorus diets for 68-week laying hens; either source of phytase can be fed to commercial 68-week laying hens at 500 FTU/kg to correct the negative effects associated with a 0.2% available phosphorus diet. In conclusion, either source of phytase can be fed to commercial first cycle laying hens at 500 FTU/kg to effectively replace inorganic phosphorus when economically justified.

Effects of Feeding High and Low Ca Additive on Laying Performance and Egg Quality in Laying Hens (산란계 사료 내 칼슘의 수준별 첨가 급여가 생산성 및 계란 품질에 미치는 영향)

  • Kang, Hwan-Ku;Kang, Geun-Ho;Kim, Dong-Wook;Na, Jae-Cheon;Yu, Dong-Jo;Lee, Sang-Jin;Kim, Sang-Ho
    • Journal of Animal Science and Technology
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    • v.50 no.6
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    • pp.799-806
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    • 2008
  • This experiment was carried out to investigate the effect of feeding limestone to high and low Ca layer diets on laying performance and eggshell quality in laying hens. Five hundred and twenty-eight HyLine brown layer allotted to six treatments consisting of high and low Ca diets. Limestone added to two kinds of basal diet with 1, 2 and 3 fold compared to conventional diet. Egg production was not different regardless Ca level and amount of added limestone in overall periods. However, the increment of egg observed in high Ca diet and added 10g limestone group during later period. Ca and limestone intake were increased as increasing feeding limestone. Eggshell strength and thickness were improved in high Ca consumption group. It was concluded that feeding Ca sources to conventional diets was helpful improving eggshell quality and reducing feed cost per egg mass by increasing proportion of Ca intake.

Position Control of Micro Particles in a Fluid Flow Using Ultrasonic Standing Wave (정재초음파를 이용한 유동중 미세 입자 위치 제어)

  • Cho, Seung-Hyun;Seo, Dae-Cheol;Ahn, Bong-Young;Kim, Ki-Bok;Kim, Yong-Il
    • Journal of the Korean Society for Nondestructive Testing
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    • v.28 no.2
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    • pp.131-136
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    • 2008
  • Using ultrasonic standing waves, micro particles submerged or flowing in fluid can be manipulated. Due to acoustic radiation force of ultrasound, particles are forced to move to pressure nodal or antinodal lines. In this work, we propose a method to control the position of micro particle in a flow by adjusting the frequency of the standing wave. To this end, standing wave field generation system including a few millimeter thick micro channel was established using an immersible ultrasonic transducer. The present generation system works valid in a frequency range between 2.0 MHz and 2.5 MHz. We observed the SiC particles in water moved to pressure nodal lines by the standing wave. The effect of the channel thickness and operating frequency was also investigated. Interestingly, it was shown that the operating frequency have a close relation with the location of the pressure nodal line. Consequently, it fan be said that the position of particle movement rail be controlled by adjusting the ultrasound frequency. The maximum range of the controllable position was about 261 micrometers under the given condition. The resulted observations reveal the possibility of various applications of the ultrasonic standing wave to the manipulation of particles submerged in a fluid.

Effects of an Unexpected Change in Housing Environment on Stress in Poultry (급격한 사육환경의 변화가 가금의 생산성과 스트레스에 미치는 영향)

  • Kim, Jimin;Yoon, Hyung-sook;Hwangbo, Jong;Kim, Sang-Ho;Choi, Yang-Ho
    • Journal of Animal Environmental Science
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    • v.18 no.3
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    • pp.183-190
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    • 2012
  • The objective of this study was to evaluate the effects of an unexpected change in housing environment on stress in poultry. Laying hens (Hy-line Brown), adapted to a free-range housing system for more than a month, were randomly divided into two groups to be subjected to an unexpected change of their housing environment: one half of them were individually housed into conventional battery cages and the other continually left in the same housing system throughout the entire period of the study. The sudden change resulted in an increase in egg production and albumen height (P<0.05), but decrease in eggshell thickness (P<0.05). The change had tendency to increase Haugh unit ($P{\leq}0.061$) and to decrease eggshell color ($P{\leq}0.074$), but did not affect body weight for 5 days thereafter. No significant changes were detected in liver color. Plasma concentrations of corticosterone was acutely increased one day after the change (P<0.05), then returned to similar control levels. The results of the present study indicate that unexpected changes in housing environment cause acute increase in stress hormone concentrations, but interpretation of the results should be cautious due to the experimental conditions.

Effect of a Bonding Layer between Electrodes on the Performance of a λ/4-Mode PVDF Ultrasound Transducer (λ/4 모드 PVDF 초음파 트랜스듀서에 있어서 전극 사이의 접합층이 성능에 미치는 영향)

  • Cao, Yonggang;Ha, Kanglyeol;Kim, Moojoon;Kim, Jungsoon
    • The Journal of the Acoustical Society of Korea
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    • v.33 no.2
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    • pp.102-110
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    • 2014
  • The effect of a bonding layer on the performance of a quarter-wave (${\lambda}/4$) mode PVDF ultrasound transducer having not only a piezoelectric layer but also a non-piezoelectric layer between two electrodes was analyzed. The equivalent circuit of a transmission line model by Kikuchi et al.[Sound of IEICE, 55-A, 331-338 (1981)] was introduced for the analysis. The validity of the model was confirmed by comparison with a KLM model for three postulated adhesion cases of a $80{\mu}m$ thick piezoelectric PVDF film to a copper (Cu) backer. The pulse-echo responses of five PVDF transducers, each fabricated with a different thickness ($5{\mu}m{\sim}20{\mu}m$) of the bonding layer, were measured and the results were compared with those by simulation. The two results were in good agreement with each other and it was noted that the effect of the bonding layer on the performance of the transducer could be analyzed by the Kikuchi model. In detail, the $20{\mu}m$ bonding layer decreased the center frequency and the bandwidth by about 19.7 % and 25.0 %, respectively, and increased the insertion loss by 57.2 %.