• Nutrition Research and Practice
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• 제10권4호
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• pp.393-397
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• 2016

BACKGROUND/OBJECFTIVES: Artemisinin, a natural product isolated from Gaeddongssuk (artemisia annua L.) and its main active derivative, dihydroartemisinin (DHA), have long been used as antimalarial drugs. Recent studies reported that artemisinin is efficacious for curing diseases, including cancers, and for improving the immune system. Many researchers have shown the therapeutic effects of artemisinin on tumors such as breast cancer, liver cancer and kidney cancer, but there is still insufficient data regarding glioblastoma (GBM). Glioblastoma accounts for 12-15% of brain cancer, and the median survival is less than a year, despite medical treatments such as surgery, radiation therapy, and chemotherapy. In this study, we investigated the anti-cancer effects of DHA and transferrin against glioblastoma (glioblastoma multiforme, GBM). MATERIALS/METHODS: This study was performed through in vitro experiments using C6 cells. The toxicity dependence of DHA and transferrin (TF) on time and concentration was analyzed by MTT assay and cell cycle assay. Observations of cellular morphology were recorded with an optical microscope and color digital camera. The anti-cancer mechanism of DHA and TF against GBM were studied by flow cytometry with Annexin V and caspase 3/7. RESULTS: MTT assay revealed that TF enhanced the cytotoxicity of DHA against C6 cells. An Annexin V immune-precipitation assay showed that the percentages of apoptosis of cells treated with TF, DHA alone, DHA in combination with TF, and the control group were $7.15{\pm}4.15%$, $34.3{\pm}5.15%$, $66.42{\pm}5.98%$, and $1.2{\pm}0.15%$, respectively. The results of the Annexin V assay were consistent with those of the MTT assay. DHA induced apoptosis in C6 cells through DNA damage, and TF enhanced the effects of DHA. CONCLUSION: The results of this study demonstrated that DHA, the derivative of the active ingredient in Gaeddongssuk, is effective against GBM, apparently via inhibition of cancer cell proliferation by a pharmacological effect. The role of transferrin as an allosteric activator in the GBM therapeutic efficacy of DHA was also confirmed.

• 대한한의학회지
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• 제29권4호
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• pp.133-145
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• 2008

Objectives: The aim of present study was to investigate recovery effects of Hirudo, which has been used clinically in diabetes therapy. Methods: We established three groups: normal, control, Hirudo, and assigned 6 rats to each group. The normal group was not treated by any process and fed by normal saline. The control & Hirudo groups were administered streptozotocin (STZ) to induce diabetes. Hirudo extract was orally administered to the Hirudo group for 10 days. After 8 weeks, the rats were sacrificed and their body weight, 24hrs urinary protein excretion, glucose, albumin, BUN, creatinine, total-cholesterol, LDL-cholesterol, triglyceride in blood, and level of glycation end-product (AGE) and transforming growth factor (TGF-${\beta}1$) in serum were measured. Morphological profiles and morphometric studies of the kidney cortex, renal transforming growth factor (TGF-${\beta}1$) expression, and renal receptor for advanced glycation end-products (RAGE) expression were studied. Results: The following results were obtained. The protein amount in urine per 24hrs of the Hirudo-treated group as compared to the control group was significantly reduced. The BUN and creatinine level in serum of the Hirudo-treated group as compared to the control group was significantly inhibited. The construction change in kidney of the Hirudo-treated group as compared to the control group was significantly inhibited. The factor of the Hirudo-treated group as compared to the control group was significantly inhibited, which induced the construction change in kidney. Conclusions: The above results suggest that Hirudo partially improved the function of the kidney.

• Tuberculosis and Respiratory Diseases
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• 제57권2호
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• pp.193-196
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• 2004

저자들은 실리콘 액을 이용한 질 성형술 후에 급성호흡 곤란 증후군을 보인 환자에서 경기관지 폐생검으로 실리콘에 의한 것으로 생각되는 폐포 대식세포내의 봉입체를 확인하고 대증적인 치료로 호전된 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다.

• KSBB Journal
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• 제16권6호
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• pp.579-591
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• 2001

The cell growth inhibitor effect of cervical cancer cells was investigated by liposome mediated transfection (pRcCMVp53/lipofectin) and by transfection using adenovirus (AdCMVp57). The papilloma virus cancer cell lines we used in this study were HPV16 positive, having inhibiter gene, wild p53 gene, CaSki, SiHa, HPV18 positive HeLa, HeLaS3 and HPV negative C33A, HT3. LacZ gene of E.coli was used as the marker gene for the transfection efficiency. The effect on the inhibition of tumor cell growth was measured by cell count and cell viability though ELISA analysis and MTT assay. The inhibition of tumor cell growth was confirmed by measuring each assay for six days, comparing with the normal control cell growth. The cell growth of cervical cancer calls by transfection was significantly reduced and showed tittle differences among the cell lines. To eliminate the potential problem of Ad(adenovirus) contamination during rAAV production, rAAV can be produced by a triple transfection of vector plasmic, packaging plasmid, and adenovirus helper plasmid. To examine the helper functions of Ad plasmids on the production of rAAV vector, we carried out cotransfection of three plasmids, AAV vector, packaging construct, and Ad helper plasmids. The optimized transfection condition for calcium phosphate method is 25ug of total DNA per 10-cm-diameter plate of 293 cell. We found that rAAV yields peaked at 48hr after Ad infection. The titer of rAAV was measured by the dot blot analysis to measure the number of particles/ml based on the quantification of viral DNA. Recent1y, Kombucha(fungi) was identified as a very potent antileukefic agent. In the present study, effect of natural toxin(plankton) and Kombucha is PSP(GTXI-3, neoSTX), on various MTT assay cervical cancer cell line. Toxin(GTX 1-3, neoSTX) also inhibited the proliferation in primary cervical cancer calls in a dose-dependent toxin concentration. These results showed that toxin was very potent in inhibiting the proliferation of cervical cancer calls in vitro. Toxins and Kombuoha exhibited a dose dependent inhibition of cellular proliferation in cancer cell line.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2005년도 제22차 정기총회 및 학술발표회
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• pp.31-43
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• 2005

Isoflavones are naturally occurring plant chemicals belonging to the 'phytoestrogen' class. The isoflavones are strikingly similar in chemical structure to natural estrogens. The phenolic ring is a key structural element of most compounds that bind to estrogen receptors. Dietary components that recently have received attention for their action as phytoestrogens are soy isoflavones. Soy products are the most significant dietary sources of isoflavones. Recently It is concerned clinical nutrition of isoflavone that is driven by reason of alternative sources of exogenous estrogen are constantly being needed. Estrogen therapy after the menopause offers protection from cardiovascular disease, reduces the extent of osteoporosis and relieves menopausal symptoms. Exogenous estrogen treatment is a fear of possible increased risk of developing breast cancer and because of side effects. Daily intake of soybean or soy food can affirmative effect to disease occurrence, that is based on mechanical investigation, experimental results of animals and human. Research into isoflavone is going on various field to relieve hormone - dependent disease such as cancer, menopausal symptom, cardiovascular disease and osteoporosis. Isoflavone is plenty in soybean meal, soy by-product, but only limited information is available on isoflavone efficacy into animal husbandry. Thus we conducted three experiments to investigate the effects of dietary isoflavone on productivities, antioxidative responses and bone metabolism in poultry. Dietary supplementation of isoflavone resulted in preventing the lipid oxidation of plasma and egg yolk. Dietary isoflavone improved bone development in egg-type growing chicks and broilers in terms of tibial strength. It was suggested that the proper use of feed additives such as isoflavone might provide means of improving antioxidative effect, skeletal strength, egg and eggshell quality.

• 혜화의학회지
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• 제25권1호
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• pp.155-163
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• 2016

Objectives : Since the Korean pronunciation of the Mantidis Oothea is not unified, it is necessary to unify pronunciation of this particular herb. According to ancient herbal literature, genuine Mantidis Oothea has been regarded as the product which is attached to mulberry tree. However, all forms of Mantidis Oothea need to be used as a medicinal ingredient. Methods : The current study explores pronunciations of the herbal medicinal names, Collection, and the herbal processes of the Mantidis Oothea. There are approximately about 30 herbal manuals dealing with the Mantidis Oothea after Shen Nong's herbal manual(神農本草經) was published. Results & Conclusions : 1. Sangbicho, Sangpyeojo, Sangpyeocho, and other names have been used as a name of medicinal ingredient for the Mantidis Oothea. 2. The Other names of medicinal ingredient for the Mantidis Oothea were Sik woo, Danglangja(螳螂子), Danglanglan(螳螂卵), Danglangso(螳螂巢), Danglangwa(螳螂窩), Danglanggag(螳螂殼), etc.. 3. The name of medicinal ingredient for the Mantidis Oothea should not be pronounced as Sangpyoso but Sangpyocho. 4. The name of medicinal ingredient for the Mantidis Oothea was originated from a type of the egg case, eating habit, therapy of the Mantidis Oothea and so on. 5. Collecting real thing of The Mantidis Oothea does not really matter whether it is attached to the mulberry tree or not. 6. The herbal processes of the Mantidis Oothea is to kill the eggs, so typical methods were to steam, roast, boil with vinegar and so on. In addition, the main effect seemed to be protection from the diarrhea.

• Natural Product Sciences
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• 제13권1호
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• pp.17-22
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• 2007

Fresh unripe whole fruits of Luffa tuberosa (Roxb.) or Momordica tuberosa (Roxb.) Cogn. (Cucurbitaceae) were evaluated for the antidiabetic and hyperlipidemic potential in streptozotocin (STZ) induced diabetic rats. Diabetes was induced by administration of intra-peritoneal injection of streptozotocin at a dose of 55 mg/kg body weight. After the induction of diabetes aqueous extract of L.tuberosa (AELT) was administered orally at doses of 250 and 500 mg/kg. body weight/day for a period of 14 days. The Fasting blood glucose (FBG) levels, serum insulin levels, changes in body weight, food and liquid intake were measured. In diabetic rats, the AELT exhibited significant reduction in blood glucose levels. Biochemical assay of plasma total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDLc), low-density lipoprotein cholesterol (LDLc), glycogen content and glucose-6-phosphatase activity in liver were assessed. Antihyperlipidemia in diabetic rats after the extract supplementation was confirmed by significant reduction in the levels of above mentioned hyperlipidemic indicators. This study focus on the efficacy of L.tuberosa fruits for the management of experimental STZ induced diabetic rats and provides the scientific basis of ancient herbal therapy and use of these fruits as vegetable.

• 농촌지도와개발
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• 제24권3호
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• pp.173-183
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• 2017

The purpose of this study was to identify differences by business motivation of agro-healing farm s in Korea. A survey was conducted for entrepreneurs in agro-healing farmers with self-administered questionnaires. Main results of this study were as follows: First, business motivation of agro-healing farm s in Korea can be classified to 1) education-oriented, 2) care-oriented, and 3) sale revenue-oriented motivation. Second, care-oriented farms provide healing services to people in need like the disabled. These farms may be required a long-term program such as residence-required format. And these farms need supports of health and medical service personnel or institutions. Third, sale revenue-oriented farms have sources of main income from agricultural products or processed goods made in these farms. Therefore, these farms may be required a business strategy such as product development, marketing rather than agro-healing programs. fourth, the estimation of a Multinominal logistic regression model determines the characteristics of agro-healing entrepreneurs who are most likely to opt for each type motivation of participating for agro-healing farm business. the most important determinants on business motivation of agro-healing farms were 'participants type in programs', 'program operating time', 'supporting health and medical service personnel or institutions' and 'program revenue ratio in farm operating revenue'.

• 생약학회지
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• 제43권4호
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• pp.323-327
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• 2012

Acne, also known as Acne vulgaris, is a common disorder of human skin involving the sebaceous gland and Propionibacterium acnes (P. acnes). The purpose of this study was to demonstrate whether anti-acne herbal complex (AAHC), a functional extract from herbal complex can be used for acne treatment as a natural product. We first demonstrated anti-acne activity of AAHC in mouse acne model. Acne was induced by injecting P. acnes on the backside $2{\times}10^7$ CFUs in ICR mice and then the mice were treated with AAHC by dermal application once daily. ACFREE$^{(R)}$ (clindamicin phosphate) was used as a positive control. Treatment with AAHC decreased the P. acnes-induced skin swelling and inflammation. AAHC treatment significantly decreased serum DHT concentration in acne-induced mice. Especially, treatment of 20% AACH in mice was more effected than 40%. We next evaluated the antimicrobial property of AAHC against P. acnes, Staphylcococcus aureus (S.aureus), and Escherichia coli (E. coli). Incubation of P. acnes, S. aureus, and E. coli with AAHC showed minimal inhibitory concentration (MIC) values against the bacterial growth lower. Alamar blue method was also carried for the antibacterial activity. It was effectively MIC level at 6.25% of P. acnes. AAHC effectively inhibited the growth of S. aureus and E. coli at 0.097% on MIC level, respectively. Our results showed the potential of using AAHC as an alternative treatment for antibiotic therapy of acne and the application of AAHC as a herbal medicine for acne treatment.

• 동의생리병리학회지
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• 제25권4호
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• pp.596-602
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• 2011

The aim of this study is to investigate the effects of onion and acanthopanax senticosus on body weight change, serum total cholesterol, HDL cholesterol, LDL cholesterol, triglyceride, free fatty acid, total lipid, phospholipid level, renal and liver function test, and CBC in obese rats and mice. Obese rats induced by high-fat fed are medicated for 7 weeks. Rats are divided into four groups depending on the medication; normal group (general-fat fed and no-medication), control group (high-fat fed and no medication), sample A group (high-fat fed and onion 100% extracted medication), sample B group (high-fat fed and onion 50% & acanthopanax senticosus 50% extracted medication), sample C group (high-fat fed and red onion 50% & acanthopanax senticosus 50% extracted medication). After medication, obesity related index, renal and liver function test, and CBC are analysed. There are significant statistical differences among control group and all experimental groups for the body weight change. There are significant statistical differences among control groups and all experimental groups for the total cholesterol, HDL cholesterol, LDL cholesterol, triglyceride level, free fatty acid, and phospholipid level. These results suggest that medications of onion and acanthopanax senticosus extracted products are effective for the treatment of obesity. Especially, onion 100% extracted product is more effective than the others.