• 한국해양학회지
• /
• 제6권2호
• /
• pp.78-84
• /
• 1971

A year-long survey of bacteriological water quality for Lake Eui-am in Kang- won Province, Korea, was conducted from June 1970 to May 1971. the purpose of this investigation was: 1) to determinate the seasonal prevalence of fecal pllution bacteria, such as coliforms, fecal coliforms and enterococci, in Lake Eui-am; 2) to correlate these findings with associated microbiological parameters; and 3) to interpret these results with respect to water quality and environmental health. The membrane filter techniques were used, for the determination of these bacteria.Densities of total coliforms, fecal coliforms and enterococci exhibited seasonal variations, the numers of these fecal pollution bacteria being high in summer and fall months in close possitive relation to the amount of rainfall, and being low winter and spring months. On the whole, the level of fecal pollution bacteria in Lake Eui-am is yet quite low There were not any evident correlation among the density of these pollution bacteria. The ratio of fecal coliforms to enterococci of the lake water varied from 0.01 to 4.25 with average of 1.47.

• Journal of Microbiology and Biotechnology
• /
• 제21권6호
• /
• pp.659-664
• /
• 2011

Staphylococcus aureus (SA) bacteremia is associated with high mortality, and often results in metastatic infections. The methicillin-resistant SA (MRSA) is an urgent health care issue, as nosocomial infections with these bacteria represent limited treatment alternatives. Samples of whole blood containing challenge inoculums of SA and MRSA strains were passed through columns packed with surfaceheparinized polyethylene beads. The bound bacteria were eluted and quantitatively determined by culturing and by real-time PCR. Significant amounts of both SA and MRSA adhered to the heparinized beads (more than 65% of inoculated bacteria). After rinsing with buffer at high ionic strength, viable bacteria or bacterial DNA were eluted from the columns, indicating that the binding was specific. The conclusions that can be made from these experiments are that, as earlier reported in the literature, the high affinity of SA to heparin is retained in whole blood, and MRSA in whole blood binds to heparin with similar or higher affinity than SA. It should be possible to lower the amount of SA and/or MRSA from the blood of infected patients to levels that could be taken care of by the immune system. In previous studies, we have shown that passing blood from septic patients over beads coated with end-point-attached, biologically active heparin is a useful technique for regulating the levels of heparinbinding cytokine. These findings in combination with the present findings indicate the possibility of creating an apheresis technology for treatment of sepsis caused by SA and/or MRSA.

• Animal Bioscience
• /
• 제34권4호
• /
• pp.584-593
• /
• 2021

Objective: The objective of this study was to compare fenugreek (FG) with alfalfa (Alf) in ruminal fermentation and methane (CH4) production in vitro. Methods: Whole-plant FG harvested at 11- and 15-wk and Alf harvested at early and mid-bloom maturities, alone or as 50:50 mixture of FG and Alf at the respective maturity, were assessed in a series of 48-h in vitro batch culture incubations. Total fermentation gas and methane gas production, dry matter (DM) disappearance, volatile fatty acids, microbial protein and 16S RNA gene copy numbers of total bacteria and methanogens were determined. Results: Compared to early bloom Alf, FG harvested at 11-wk exhibited higher (p<0.05) in vitro DM and neutral detergent fibre disappearance, but this difference was not observed between the mid-bloom Alf and 15-wk FG. Regardless plant maturity, in vitro ruminal fermentation of FG produced less (p<0.001) CH4 either on DM incubated or on DM disappeared basis than that of Alf during 48-h incubation. In vitro ruminal fermentation of FG yielded similar amount of total volatile fatty acids with higher (p<0.05) propionate percentage as compared to fermentation of Alf irrespective of plant maturity. Microbial protein synthesis was greater (p<0.001) with 11-wk FG than early bloom Alf as substrate and 16S RNA gene copies of total bacteria was higher (p<0.01) with 15-wk FG than mid-bloom Alf as substrate. Compared to mid-bloom Alf, 15-wk FG had lower (p<0.05 to 0.001) amount of 16S RNA methanogen gene copies in the whole culture during 48-h incubation. Conclusion: In comparison to Alf, FG emerges as a high quality forage that can not only improve rumen fermentation in vitro, but can also remarkably mitigate CH4 emissions likely due to being rich in saponins.

• 한국식품영양과학회지
• /
• 제23권1호
• /
• pp.73-77
• /
• 1994

This study was conducted to investigate and make comparison between treatment which was reduced the reducing sugar content from Chinese cabbage using salting and desalting processes prior to Kimchi fermentation , and control for the effect of reducing sugar content on Kimchi fermentation at $25^{\circ}C$. In the early stage of Kimchi fermentation , the amount of reducing sugar (5.7mg/ml) in treatment was much smaller than that (15.1mg/ml) in control. Reducing sugar content of treatment decreased drastically during the first two days and then levelled off . Whereas, that of control dropped significantly up to the first four days of fermentation. pHs of treatment and control decreased significantly during the first two days and then showed gentle slopes. Acidities of treatment and control were increased continuously during the entire range of fermentation . The acidity of control reached to 0.75% in 3 days of fermentation, while that of treatment was shown after 6 days. Hardnesses of treatment and control using a puncture test were almost constant and the hardness value of treatment was higher than that of control during whole fermentation period. The total bacteria and lactic acid bacteria counts increased drastically during the first day of fermentation and the increase of total bacteria counts was mainly caused by that of lactic acid bacteria counts.

• 한국철도학회:학술대회논문집
• /
• 한국철도학회 2002년도 춘계학술대회 논문집
• /
• pp.549-554
• /
• 2002

Recently, interest in hygiene has been arouse in the health care field. Consequently, Filters with antibacterial agent applied to improve air quality by sterilizing bacteria, fungi, etc. We actually installed antibacterial air filter containing 2.5wt%Ag zeolite on the air intake route to air-conditioner in passenger cu, and evaluated filter's performance on antibacterial effect. By the microbe liquid spattering method, we found that the antibacterial air filter has notably sufficient antibacterial efficiency against standard strains and wild type strains. Antibacterial effect was observed at whole area of filter media by zone of inhibition test. The evaluation of microbe quantity was conducted through mixing dilution plate culture method. In comparison with ordinary filter, the amount of germs attached on antibacterial air filters was larger. The amount of germs attached on ordinary filters was very small since ordinary filters contained less dust. In comparison in antibacterial air filter with thickness, the amount of germs attached on 9mm filter was smaller than that of on 6mm filter. i.e. thicker filter, superior efficiency.

• 미생물학회지
• /
• 제21권3호
• /
• pp.143-148
• /
• 1983

Streptococcus sanguis와 여타 구강세균의 PZ-peptidase의 생산을 연구하였다. 세척한 온전한 세균세포를 효소윈으로, 그리고 PZ-pentapeptide를 효소의 기질로 사용하였다. 이 연구에서 책택한 균의 배양조건에서, S. sanguis에서는 넓은 범위의 효소활성도가, 실험실 균주와 신선한 분리균주에서 검출되었는데, 그 값은 O.5~7.9Unit/mg protein 이었다. Streptococcus mµtans와 Lactobacilli는 낮은 효소활성을 보였고 S. mutans의 경우 그 값은 0~0.5Unit/mg protein이었다. Streptococcus mitis와 Streptococcus salivarius는 다른 세균과 비교할 때 중등도의 효소활성을 갖고 있었고, Actiηomyces의 균주들은 S. sanguis처럼 넓은 범위의 활성도 (0~9.8 unit/mg protein)를 지니고 있었다. 본 논운에서 취급한 구강세균이 생성하는 PZ-peptidase가 사람의 타액단백질의 분해에 참여할 수 있는 가능성을 더불어 고찰하였다.

• 한국유기농업학회지
• /
• 제25권2호
• /
• pp.311-328
• /
• 2017

두둑과 고랑을 재활용한 한국형 무경운 농업에서 유기물 투입과 관수 효과를 구명하고자 무경운 토양에서 시험을 수행하였다. 1. 토양 미생물상 1회 전량관수 조건에서 대두박 투입 처리구의 토양 세균과 곰팡이 수는 대두박 무 투입구에 비하여 많았다. 그리고 유기질비료 투입량이 표준시비량 66%까지 증가되면 세균과 곰팡이 수는 증가되었으나, 그 이상에서는 세균과 곰팡이 수가 감소되는 경향이었다. 곰팡이/세균 비율은 관수 방법과 관계없이 대두박 투입 처리에서 0.6과 1.1로, 무투입 처리의 0.2와 0.5보다 2배 이상 높았다. 1회 전량 관수 조건에서 유기질 비료 시비량이 증가되면 대두박을 투입한 처리는 방선균 수는 감소되는 경향이었으나, 대두박 무투입에서는 증가되었다. 2회 분할 관수는 1회 전량관수에 비하여 대두박 무 투입 조건에서 세균과 곰팡이 수가 증가되었으나, 대두박 투입조건에서는 방선균 수가 증가되었다. 2. 토양 효소 유기질 비료의 시비량이 증가되면 토양 내 Chitinase 활성은 대두박 투입 토양에서 감소되고, 대두박 무 투입에서는 증가되는 경향이었다. 그러나 대두박을 투입에 관계없이 2회 분할 관수는 1회 전량관수에 비하여 Chitinase 활성이 증가되었다. 1회 전량관수 조건에서 대두박 투입 처리구의 ${\beta}$-Glucosidase 활성은 무투입에 비하여 높았으며, 유기질 비료 투입량이 증가되면 표준시비량의 66%까지는 ${\beta}$-Glucosidase 활성이 증가되었으나, 표준시비량에서는 감소되었다. 대두박 무투입 조건에서 2회 분할관수 토양 내 ${\beta}$-Glucosidase 활성은 1회 전량관수에 비하여 현저하게 증가되었다. 1회 전량관수 조건에서 대두박을 투입한 처리의 N-acetyl-${\beta}$-D-glucosaminidase의 활성은 무투입구에 비하여 높았다. 대두박 투입 처리에서 유기질 비료 투입량이 표준시비량의 66%까지 증가되면 N-acetyl-${\beta}$-D-glucosaminidase의 활성은 증가되었으나, 표준시비량에서는 유의적인 차이가 없었다. 대두박 무투입 조건에서 2회 분할관수는 1회 전량관수에 비하여 N-acetyl-${\beta}$-D-glucosaminidase의 활성은 증가되었다. 대두박 무투입 조건에서 유기질 비료 시비량이 표준량의 66% 수준에서는 토양 내 산성인산가수분해효소(Acid phosphatase)의 활성 높았다. 대두박 투입 조건에서는 유기질 비료 시비량이 증가되면 산성인산가수분해효소(Acid phosphatase)의 활성은 증가되는 경향이었다. 3. 토양 AMF 대두박 무투입 조건에서 유기질 비료의 투입량이 표준시비량의 66%까지 증가되면 토양의 내생균근균의(AMF) 포자수는 증가되었으나, 유기질 비료 투입량이 표준시비량에서는 근균의 포자수는 감소되었다. 그러나 대두박 투입에서 근균의 포자수는 유기질 비료 투입량에 따른 유의적인 차이가 없었다. 그리고 내생 근균의 고추 뿌리에 정착률은 대두박 투입량에 따른 유의적인 차이가 없었으며, 2회 분할 관수도 같은 경향이었다.

• 상하수도학회지
• /
• 제11권4호
• /
• pp.43-53
• /
• 1997

In this research, the synthetic livestock wastewater was prepared to study the characteristics of organic matter removal, the change of VFA production, and the amount of gas production with respect to the change of ammonium nitrogen concentration in the waste using anaerobic fixed bed process, which is an anaerobic biofilm process. The HRT and operation temperature were 1 day and $35{\pm}1^{\circ}C$, respectively. Also, the characteristics of organic matter removal and the inhibitory effect on microorganism in the anaerobic process were studied on the organic loading and ammonium nitrogen concentration. The results obtained were as follows: For COD loading of $10kg/m^3$-day and five levels of ammonium nitrogen concentration ranging from 1,000 to 5,000 mg/L, organic removal efficiencies were about 81, 74, 67, 58, and 51%, and gas productions were 3,860, 3,520, 3,240, 3,020, and 2,790 ml/l-day, respectively. Average methane contents in the gas produced on COD loading of $10kg/m^3$-day was about 76%. Throughout the whole period of experiment, remaining VFA (as COD base) in the effluent was over 90% of remaining COD. This result indicated the inhibitory effect of high concentration of ammonium nitrogen through the facts that accumulated VFA was almost COD and organic removal efficiency decreased also with the increase of ammonium nitrogen. Especially, that implys which high concentration of ammonium nitrogen not only inhibits methane forming bacteria, but also acid forming bacteria.

• Journal of Photoscience
• /
• 제9권2호
• /
• pp.364-366
• /
• 2002

Photoadaptation of Chlorobium (Cb.) phaeobacteroides was investigated under dim and strong light intensity. Absorption spectra of these whole cellIs were different each other. The Soret band intensity and the Qy bandwidth of BChl e in c디l grown under dim light intensity were smaller and more broadened than those under strong light intensity. From HPLC analysis of the pigments, total carotenoid (Car) / bacterochorolphyll (BChl) e ratio of cell increased wi1h increase of light intensities. But camposition of BChl e hamologs almost unchanged. Cb. phaeobacteroides contains 11 kinds of Car including isorenieratene and beta-isorenieratene as major Car. The campositions of Car were different for cells grown under dim and strong light intensities. In conclusion, Cb. phaeobacteroides changes total amount and canposition of Car to adapt various light intensities, while homolog canposition of BChle unchange.

• BMB Reports
• /
• 제37권1호
• /
• pp.83-92
• /
• 2004

As a result of the enormous amount of information that has been collected with E. coli over the past half century (e.g. genome sequence, mutant phenotypes, metabolic and regulatory networks, etc.), we now have detailed knowledge about gene regulation, protein activity, several hundred enzyme reactions, metabolic pathways, macromolecular machines, and regulatory interactions for this model organism. However, understanding how all these processes interact to form a living cell will require further characterization, quantification, data integration, and mathematical modeling, systems biology. No organism can rival E. coli with respect to the amount of available basic information and experimental tractability for the technologies needed for this undertaking. A focused, systematic effort to understand the E. coli cell will accelerate the development of new post-genomic technologies, including both experimental and computational tools. It will also lead to new technologies that will be applicable to other organisms, from microbes to plants, animals, and humans. E. coli is not only the best studied free-living model organism, but is also an extensively used microbe for industrial applications, especially for the production of small molecules of interest. It is an excellent representative of Gram-negative commensal bacteria. E. coli may represent a perfect model organism for systems biology that is aimed at elucidating both its free-living and commensal life-styles, which should open the door to whole-cell modeling and simulation.