Browse > Article
http://dx.doi.org/10.5713/ajas.20.0114

In vitro ruminal fermentation of fenugreek (Trigonella foenum-graecum L.) produced less methane than that of alfalfa (Medicago sativa)  

Niu, Huaxin (College of Animal Science and Technology, Inner Mongolia University for Nationalities)
Xu, Zhongjun (Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada (AAFC))
Yang, Hee Eun (Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada (AAFC))
McAllister, Tim A (Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada (AAFC))
Acharya, Surya (Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada (AAFC))
Wang, Yuxi (Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada (AAFC))
Publication Information
Animal Bioscience / v.34, no.4, 2021 , pp. 584-593 More about this Journal
Abstract
Objective: The objective of this study was to compare fenugreek (FG) with alfalfa (Alf) in ruminal fermentation and methane (CH4) production in vitro. Methods: Whole-plant FG harvested at 11- and 15-wk and Alf harvested at early and mid-bloom maturities, alone or as 50:50 mixture of FG and Alf at the respective maturity, were assessed in a series of 48-h in vitro batch culture incubations. Total fermentation gas and methane gas production, dry matter (DM) disappearance, volatile fatty acids, microbial protein and 16S RNA gene copy numbers of total bacteria and methanogens were determined. Results: Compared to early bloom Alf, FG harvested at 11-wk exhibited higher (p<0.05) in vitro DM and neutral detergent fibre disappearance, but this difference was not observed between the mid-bloom Alf and 15-wk FG. Regardless plant maturity, in vitro ruminal fermentation of FG produced less (p<0.001) CH4 either on DM incubated or on DM disappeared basis than that of Alf during 48-h incubation. In vitro ruminal fermentation of FG yielded similar amount of total volatile fatty acids with higher (p<0.05) propionate percentage as compared to fermentation of Alf irrespective of plant maturity. Microbial protein synthesis was greater (p<0.001) with 11-wk FG than early bloom Alf as substrate and 16S RNA gene copies of total bacteria was higher (p<0.01) with 15-wk FG than mid-bloom Alf as substrate. Compared to mid-bloom Alf, 15-wk FG had lower (p<0.05 to 0.001) amount of 16S RNA methanogen gene copies in the whole culture during 48-h incubation. Conclusion: In comparison to Alf, FG emerges as a high quality forage that can not only improve rumen fermentation in vitro, but can also remarkably mitigate CH4 emissions likely due to being rich in saponins.
Keywords
Fenugreek; Alfalfa; In vitro; Methane; Rumen Bacteria;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Doepel L, Montgomery JE, Beauchemin KA, King JR, Acharya SN. Ruminal degradability and whole-tract digestibility of protein and fibre fractions in fenugreek haylage. Can J Anim Sci 2012;92:211-7. https://doi.org/10.4141/cjas2011-101   DOI
2 Abdouli H, Ayed MH, Elham M, Nabila B, Morencos MRA. Proximate composition, and total phenols, tannins, flavonoids and saponins, and in vitro ruminal fermentation activity of fenugreek cut at three maturity stages. Livest Res Rural Dev 2012;24:13.
3 Yadav UCS, Baquer NZ. Pharmacological effects of Trigonella foenum-graecum L. in health and disease. Pharm Biol 2014;52:243-54. https://doi.org/10.3109/13880209.2013.826247   DOI
4 Acharya SN, Thomas JE, Basu SK. Fenugreek, an alternative crop for semiarid regions of North America. Crop Sci 2008;48:841-53. https://doi.org/10.2135/cropsci2007.09.0519   DOI
5 Alemu AW, Doepel L. Fenugreek (Trigonella foenum-graecum L.) as an alternative forage for dairy cows. Animal 2011;5:1370-81. https://doi.org/10.1017/S1751731111000322   DOI
6 Committee on Nutrient Requirements of Small Ruminants, NRC. Nutrient requirements of small ruminants: sheep, goats, cervids, and new world camelids. 7th ed. Washington, DC, USA: National Academies Press; 2007. https://doi.org/ 10.17226/11654   DOI
7 Canadian Council on Animal Care (CCAC). CCAC guidelines on the care and use of farm animals in research, teaching and testing. Ottawa, ON, Canada: CCAC; 2009.
8 Menke KH, Raab L, Salewski A, Steingass H, Fritz D, Schneider W. The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. J Agric Sci 1979;93:217-22. https://doi.org/10.1017/S0021859600086305   DOI
9 Wang Y, Xu Z, Bach SJ, McAllister TA. Effects of phlorotannins from Ascophyllum nodosum (brown seaweed) on in vitro ruminal digestion of mixed forage or barley grain. Anim Feed Sci Technol 2008;145:375-95. https://doi.org/10.1016/j.anifeedsci.2007.03.013   DOI
10 Wang Y, Ramirez-Bribiesca JE, Yanke LJ, Tsang A, McAllister TA. Effect of exogenous fibrolytic enzyme application on the microbial attachment and digestion of barley straw in vitro. Asian-Australas J Anim Sci 2012;25:66-74. https://doi.org/10.5713/ajas.2011.11158   DOI
11 Peng K, Gresham GL, McAllister TA, et al. Effects of inclusion of purple prairie clover (Dalea purpurea Vent.) with native cool-season grasses on in vitro fermentation and in situ digestibility of mixed forages. J Anim Sci Biotechnol 2020;11:23. https://doi.org/10.1186/s40104-019-0418-6   DOI
12 Ugbogu EA, Elghandour MMMY, Ikpeazu VO, et al. The potential impacts of dietary plant natural products on the sustainable mitigation of methane emission from livestock farming. J Clean Prod 2019;213:915-25. https://doi.org/10.1016/j.jclepro.2018.12.233   DOI
13 Wang Y, McAllister TA, Yanke LJ, Cheeke PR. Effect of steroidal saponin from Yucca schidigera extract on ruminal microbes. J Appl Microbiol 2000;88:887-96. https://doi.org/10.1046/j.1365-2672.2000.01054.x   DOI
14 Tapio I, Snelling TJ, Strozzi F, Wallace RJ. The ruminal microbiome associated with methane emissions from ruminant livestock. J Anim Sci Biotechnol 2017;8:7. https://doi.org/10.1186/s40104-017-0141-0   DOI
15 Waghorn G. Beneficial and detrimental effects of dietary condensed tannins for sustainable sheep and goat production - progress and challenges. Anim Feed Sci Technol 2008;147:116-39. https://doi.org/10.1016/j.anifeedsci.2007.09.013   DOI
16 Denman SE, Tomkins NW, McSweeney CS. Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound bromochloromethane. FEMS Microbiol Ecol 2007;62:313-22. https://doi.org/10.1111/j.1574-6941.2007.00394.x   DOI
17 AOAC. Official methods of analysis of the association of official agricultural chemists. 16th ed, 5th rev. Gaithersburg, MD, USA: AOAC International; 1999.
18 McGinn SM, Beauchemin KA, Coates T, Colombatto D. Methane emissions from beef cattle: effects of monensin, sunflower oil, enzymes, yeast, and fumaric acid. J Anim Sci 2004;82:3346-56. https://doi.org/10.2527/2004.82113346x   DOI
19 Oss DB, Ribeiro GO, Marcondes MI, et al. Synergism of cattle and bison inoculum on ruminal fermentation and select bacterial communities in an artificial rumen (Rusitec) fed a barley straw based diet. Front Microbiol 2016;7:2032. https://doi.org/10.3389/fmicb.2016.02032   DOI
20 Wang Y, McAllister TA. A modified spectrophotometric assay to estimate deglycosylation of steroidal saponin to sapogenin by mixed ruminal microbes. J Sci Food Agric 2010;90:1811-8. https://doi.org/10.1002/jsfa.4019   DOI
21 Mustafa AF, Christensen DA, McKinnon JJ. In vitro and in situ evaluation of fenugreek (Trigonella foenum-graecum) hay and straw. Can J Anim Sci 1996;76:625-8. https://doi.org/10.4141/cjas96-092   DOI
22 Chaudhary S, Chaudhary PS, Chikara SK, Sharma MC, Iriti M. Review on fenugreek (Trigonella foenum-graecum L.) and its important secondary metabolite diosgenin. Not Bot Horti Agrobot Cluj Napoca 2018;46:22-31. https://doi.org/10.15835/nbha46110996   DOI
23 Farivar F, Torbatinejad NM, Jafari Ahangari Y, Hasani S, Gharebash AM, Mohajer M. In vitro evaluation of alfalfa substitution with fenugreek (Trigonella foenum graegum) hay in a high concentrate ration. Iran J Appl Anim Sci 2014;4:291-6.
24 Dey A, Paul SS, Pandey P, et al. Effect of fenugreek leaf extract (Trigonella foenum-graecum L.) on in vitro methanogenesis and fermentation of wheat straw-based diet (Triticum aestivum L.) fed to buffaloes. Sri Lanka J Food Agric 2015;1:9-13.
25 Durmic Z, Hutton P, Revell DK, Emms J, Hughes S, Vercoe PE. In vitro fermentative traits of Australian woody perennial plant species that may be considered as potential sources of feed for grazing ruminants. Anim Feed Sci Technol 2010;160:98-109. https://doi.org/10.1016/j.anifeedsci.2010.07.006   DOI
26 Goel G, Makkar HPS. Methane mitigation from ruminants using tannins and saponins. Trop Anim Health Prod 2012;44:729-39. https://doi.org/10.1007/s11250-011-9966-2   DOI
27 Jafari S, Ebrahimi M, Goh YM, Rajion MA, Jahromi MF, AlJumaili WS. Manipulation of rumen fermentation and methane gas production by plant secondary metabolites (saponin, tannin and essential oil) - a review of ten-year studies. Ann Anim Sci 2019;19:3-29. https://doi.org/10.2478/aoas-2018-0037   DOI
28 Jayanegara A, Wina E, Takahashi J. Meta-analysis on methane mitigating properties of saponin-rich sources in the rumen: influence of addition levels and plant sources. Asian-Australas J Anim Sci 2014;27:1426-35. https://doi.org/10.5713/ajas.2014.14086   DOI
29 Kamra DN, Agarwal N, Chaudhary LC. Inhibition of ruminal methanogenesis by tropical plants containing secondary compounds. Int Congr Ser 2006;1293:156-63. https://doi.org/10.1016/j.ics.2006.02.002   DOI
30 Wang Y, McAllister TA, Yanke LJ, Xu ZJ, Cheeke PR, Cheng KJ. In vitro effects of steroidal saponins from Yucca schidigera extract on rumen microbial protein synthesis and ruminal fermentation. J Sci Food Agric 2000;80:2114-22. https://doi.org/10.1002/1097-0010(200011)80:14<2114::AID-JSFA755>3.0.CO;2-0   DOI
31 Connor EE. Invited review: improving feed efficiency in dairy production: challenges and possibilities. Animal 2015;9:395-408. https://doi.org/10.1017/S1751731114002997   DOI
32 Lakhani N, Lakhani P. Plant secondary metabolites as a potential source to inhibit methane production and improve animal performance. Int J Chem Stud 2018;6:3375-9.
33 Mir Z, Acharya SN, Mir PS, et al. Nutrient composition, in vitro gas production and digestibility of fenugreek (Trigonella foenum-graecum) and alfalfa forages. Can J Anim Sci 1997;77:119-24. https://doi.org/10.4141/A96-061   DOI
34 Grossi G, Goglio P, Vitali A, Williams AG. Livestock and climate change: impact of livestock on climate and mitigation strategies. Anim Front 2019;9:69-76. https://doi.org/10.1093/af/vfy034   DOI
35 Murlidhar M, Goswami TK. A review on the functional properties, nutritional content, medicinal utilization and potential application of fenugreek. J Food Process Technol 2012;3:181.
36 Pant NC, Dhoundiyal R, Kumar M, Dwivedi U, Singh JP, Agrawal S. Fenugreek (Trigonella foenum-graecum L.) A potential source of dietary fibres and steroidal sapogenin (diosgenin). Int J Chem Stud 2018;6:612-8.