• 한국해양학회지
• /
• v.6 no.2
• /
• pp.78-84
• /
• 1971

A year-long survey of bacteriological water quality for Lake Eui-am in Kang- won Province, Korea, was conducted from June 1970 to May 1971. the purpose of this investigation was: 1) to determinate the seasonal prevalence of fecal pllution bacteria, such as coliforms, fecal coliforms and enterococci, in Lake Eui-am; 2) to correlate these findings with associated microbiological parameters; and 3) to interpret these results with respect to water quality and environmental health. The membrane filter techniques were used, for the determination of these bacteria.Densities of total coliforms, fecal coliforms and enterococci exhibited seasonal variations, the numers of these fecal pollution bacteria being high in summer and fall months in close possitive relation to the amount of rainfall, and being low winter and spring months. On the whole, the level of fecal pollution bacteria in Lake Eui-am is yet quite low There were not any evident correlation among the density of these pollution bacteria. The ratio of fecal coliforms to enterococci of the lake water varied from 0.01 to 4.25 with average of 1.47.

• Journal of Microbiology and Biotechnology
• /
• v.21 no.6
• /
• pp.659-664
• /
• 2011

Staphylococcus aureus (SA) bacteremia is associated with high mortality, and often results in metastatic infections. The methicillin-resistant SA (MRSA) is an urgent health care issue, as nosocomial infections with these bacteria represent limited treatment alternatives. Samples of whole blood containing challenge inoculums of SA and MRSA strains were passed through columns packed with surfaceheparinized polyethylene beads. The bound bacteria were eluted and quantitatively determined by culturing and by real-time PCR. Significant amounts of both SA and MRSA adhered to the heparinized beads (more than 65% of inoculated bacteria). After rinsing with buffer at high ionic strength, viable bacteria or bacterial DNA were eluted from the columns, indicating that the binding was specific. The conclusions that can be made from these experiments are that, as earlier reported in the literature, the high affinity of SA to heparin is retained in whole blood, and MRSA in whole blood binds to heparin with similar or higher affinity than SA. It should be possible to lower the amount of SA and/or MRSA from the blood of infected patients to levels that could be taken care of by the immune system. In previous studies, we have shown that passing blood from septic patients over beads coated with end-point-attached, biologically active heparin is a useful technique for regulating the levels of heparinbinding cytokine. These findings in combination with the present findings indicate the possibility of creating an apheresis technology for treatment of sepsis caused by SA and/or MRSA.

• Animal Bioscience
• /
• v.34 no.4
• /
• pp.584-593
• /
• 2021

Objective: The objective of this study was to compare fenugreek (FG) with alfalfa (Alf) in ruminal fermentation and methane (CH4) production in vitro. Methods: Whole-plant FG harvested at 11- and 15-wk and Alf harvested at early and mid-bloom maturities, alone or as 50:50 mixture of FG and Alf at the respective maturity, were assessed in a series of 48-h in vitro batch culture incubations. Total fermentation gas and methane gas production, dry matter (DM) disappearance, volatile fatty acids, microbial protein and 16S RNA gene copy numbers of total bacteria and methanogens were determined. Results: Compared to early bloom Alf, FG harvested at 11-wk exhibited higher (p<0.05) in vitro DM and neutral detergent fibre disappearance, but this difference was not observed between the mid-bloom Alf and 15-wk FG. Regardless plant maturity, in vitro ruminal fermentation of FG produced less (p<0.001) CH4 either on DM incubated or on DM disappeared basis than that of Alf during 48-h incubation. In vitro ruminal fermentation of FG yielded similar amount of total volatile fatty acids with higher (p<0.05) propionate percentage as compared to fermentation of Alf irrespective of plant maturity. Microbial protein synthesis was greater (p<0.001) with 11-wk FG than early bloom Alf as substrate and 16S RNA gene copies of total bacteria was higher (p<0.01) with 15-wk FG than mid-bloom Alf as substrate. Compared to mid-bloom Alf, 15-wk FG had lower (p<0.05 to 0.001) amount of 16S RNA methanogen gene copies in the whole culture during 48-h incubation. Conclusion: In comparison to Alf, FG emerges as a high quality forage that can not only improve rumen fermentation in vitro, but can also remarkably mitigate CH4 emissions likely due to being rich in saponins.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.23 no.1
• /
• pp.73-77
• /
• 1994

This study was conducted to investigate and make comparison between treatment which was reduced the reducing sugar content from Chinese cabbage using salting and desalting processes prior to Kimchi fermentation , and control for the effect of reducing sugar content on Kimchi fermentation at $25^{\circ}C$. In the early stage of Kimchi fermentation , the amount of reducing sugar (5.7mg/ml) in treatment was much smaller than that (15.1mg/ml) in control. Reducing sugar content of treatment decreased drastically during the first two days and then levelled off . Whereas, that of control dropped significantly up to the first four days of fermentation. pHs of treatment and control decreased significantly during the first two days and then showed gentle slopes. Acidities of treatment and control were increased continuously during the entire range of fermentation . The acidity of control reached to 0.75% in 3 days of fermentation, while that of treatment was shown after 6 days. Hardnesses of treatment and control using a puncture test were almost constant and the hardness value of treatment was higher than that of control during whole fermentation period. The total bacteria and lactic acid bacteria counts increased drastically during the first day of fermentation and the increase of total bacteria counts was mainly caused by that of lactic acid bacteria counts.

• Proceedings of the KSR Conference
• /
• 2002.05a
• /
• pp.549-554
• /
• 2002

Recently, interest in hygiene has been arouse in the health care field. Consequently, Filters with antibacterial agent applied to improve air quality by sterilizing bacteria, fungi, etc. We actually installed antibacterial air filter containing 2.5wt%Ag zeolite on the air intake route to air-conditioner in passenger cu, and evaluated filter's performance on antibacterial effect. By the microbe liquid spattering method, we found that the antibacterial air filter has notably sufficient antibacterial efficiency against standard strains and wild type strains. Antibacterial effect was observed at whole area of filter media by zone of inhibition test. The evaluation of microbe quantity was conducted through mixing dilution plate culture method. In comparison with ordinary filter, the amount of germs attached on antibacterial air filters was larger. The amount of germs attached on ordinary filters was very small since ordinary filters contained less dust. In comparison in antibacterial air filter with thickness, the amount of germs attached on 9mm filter was smaller than that of on 6mm filter. i.e. thicker filter, superior efficiency.

• Korean Journal of Microbiology
• /
• v.21 no.3
• /
• pp.143-148
• /
• 1983

The occurrence of PZ-peptidase in Streptococcus sanguis and other oral bacteria was investigated utilizing washed whole cells as the enzyme source and PZ-pentapeptide as its substrate. Under the culture conditions employed in the present study. Streptococcus sanguis strains, fresh isolates as well as laboratory strains, produced a broad range of the enzyme activity (0.5-7.9 unit/mg protein). The strains of both Streptococcus mutans and Lactobacilli showed low levels of activity (0-0.5 unit/mg protein for S. mutans). As compared with the enzyme activities of other bacteria, a moderate range of activity was produced by the strains of Strptococcus mitis nad Strptoccus salivarius. Actinomyces strains, like those of S. sanguis, produced a varying amount of activity (0-9.8 unit/ mg protein). A possible involvement of the oral bacterial PZ-peptidase in the metabolism of human saliva proteins is discussed.

• Korean Journal of Organic Agriculture
• /
• v.25 no.2
• /
• pp.311-328
• /
• 2017

This study was carried out to investigate the changes in soil microorganisms and soil enzymes by split irrigation and organic matter application under no-tillage green house conditions. Soil bacteria and fungi abundances were higher in soybean cake fertilizer than in the soil without the soybean cake fertilizer under whole quantity irrigation. Bacteria and fungi abundances in soil increased with increasing organic fertilizer application rate. Bacteria and fungi amount in the soil increased at half division irrigation in no-treatment of soybean cake fertilizer compared with whole quantity irrigation. Actinomycete amount in the soil decreased with increasing soybean cake fertilizer with whole quantity irrigation while clearly increased in no-treatment of soybean cake fertilizer. Actinomycete amount in soil clearly increased with increasing organic fertilizer input at half division irrigation. Chitinase activity in the soil decreased in soybean cake fertilizer with increasing organic fertilizer input, while increased in no-treatment of soybean cake fertilizer. Chitinase activity in the soil increased at half division irrigation compared with whole quantity irrigation regardless of soybean cake fertilizer input. ${\beta}$-Glucosidase activity in the soil was higher in soybean cake fertilizer than in no-treatment of soybean cake fertilizer with whole quantity irrigation. ${\beta}$-Glucosidase activity in the soil increased with increasing organic fertilizer input, but decreased in above the standard level 66%. ${\beta}$-Glucosidase activity in the soil clearly increased in no-treatment of soybean cake fertilizer at half division irrigation compared with whole quantity irrigation. N-acetyl-${\beta}$-D-glucosaminidase activity was higher in soybean cake fertilizer than in no-treatment of soybean cake fertilizer with whole quantity irrigation. N-acetyl-${\beta}$-D-glucosaminidase activity in the soil increased with increasing organic fertilizer input, but decreased in above the standard level 66%. N-acetyl-${\beta}$-D-glucosaminidase activity in the soil was not significantly different at half division irrigation and whole quantity irrigation in organic fertilizer input, while increased at half division irrigation in no-treatment of soybean cake fertilizer. Acid phosphatase activity increased at standard level 66% in soybean cake fertilizer, while was not significantly different in no-treatment of soybean cake fertilizer. Spore density of Arbuscular Mycorrhizal Fungi (AMF) in the soil increased with increasing organic fertilizer input at whole quantity irrigation in no-treatment of soybean cake fertilizer, while decreased above the standard level 66% in organic fertilizer input. However, spore density of AMF in the soil was not significantly different in soybean cake fertilizer regardless of input amount of organic fertilizer. Root colonization rate of AMF in red pepper roots was not significant difference at two irrigations regardless of soybean cake input.

• Journal of Korean Society of Water and Wastewater
• /
• v.11 no.4
• /
• pp.43-53
• /
• 1997

In this research, the synthetic livestock wastewater was prepared to study the characteristics of organic matter removal, the change of VFA production, and the amount of gas production with respect to the change of ammonium nitrogen concentration in the waste using anaerobic fixed bed process, which is an anaerobic biofilm process. The HRT and operation temperature were 1 day and $35{\pm}1^{\circ}C$, respectively. Also, the characteristics of organic matter removal and the inhibitory effect on microorganism in the anaerobic process were studied on the organic loading and ammonium nitrogen concentration. The results obtained were as follows: For COD loading of $10kg/m^3$-day and five levels of ammonium nitrogen concentration ranging from 1,000 to 5,000 mg/L, organic removal efficiencies were about 81, 74, 67, 58, and 51%, and gas productions were 3,860, 3,520, 3,240, 3,020, and 2,790 ml/l-day, respectively. Average methane contents in the gas produced on COD loading of $10kg/m^3$-day was about 76%. Throughout the whole period of experiment, remaining VFA (as COD base) in the effluent was over 90% of remaining COD. This result indicated the inhibitory effect of high concentration of ammonium nitrogen through the facts that accumulated VFA was almost COD and organic removal efficiency decreased also with the increase of ammonium nitrogen. Especially, that implys which high concentration of ammonium nitrogen not only inhibits methane forming bacteria, but also acid forming bacteria.

• Journal of Photoscience
• /
• v.9 no.2
• /
• pp.364-366
• /
• 2002

Photoadaptation of Chlorobium (Cb.) phaeobacteroides was investigated under dim and strong light intensity. Absorption spectra of these whole cellIs were different each other. The Soret band intensity and the Qy bandwidth of BChl e in c디l grown under dim light intensity were smaller and more broadened than those under strong light intensity. From HPLC analysis of the pigments, total carotenoid (Car) / bacterochorolphyll (BChl) e ratio of cell increased wi1h increase of light intensities. But camposition of BChl e hamologs almost unchanged. Cb. phaeobacteroides contains 11 kinds of Car including isorenieratene and beta-isorenieratene as major Car. The campositions of Car were different for cells grown under dim and strong light intensities. In conclusion, Cb. phaeobacteroides changes total amount and canposition of Car to adapt various light intensities, while homolog canposition of BChle unchange.

• BMB Reports
• /
• v.37 no.1
• /
• pp.83-92
• /
• 2004

As a result of the enormous amount of information that has been collected with E. coli over the past half century (e.g. genome sequence, mutant phenotypes, metabolic and regulatory networks, etc.), we now have detailed knowledge about gene regulation, protein activity, several hundred enzyme reactions, metabolic pathways, macromolecular machines, and regulatory interactions for this model organism. However, understanding how all these processes interact to form a living cell will require further characterization, quantification, data integration, and mathematical modeling, systems biology. No organism can rival E. coli with respect to the amount of available basic information and experimental tractability for the technologies needed for this undertaking. A focused, systematic effort to understand the E. coli cell will accelerate the development of new post-genomic technologies, including both experimental and computational tools. It will also lead to new technologies that will be applicable to other organisms, from microbes to plants, animals, and humans. E. coli is not only the best studied free-living model organism, but is also an extensively used microbe for industrial applications, especially for the production of small molecules of interest. It is an excellent representative of Gram-negative commensal bacteria. E. coli may represent a perfect model organism for systems biology that is aimed at elucidating both its free-living and commensal life-styles, which should open the door to whole-cell modeling and simulation.