• 한국철도학회:학술대회논문집
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• 한국철도학회 2010년도 춘계학술대회 논문집
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• pp.1430-1436
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• 2010

Tubular member is hollow and is an excellent source of structural member with great buckling resistance and tortional resistance. With its development and simplicity in structure, steel tubular truss has the ability to be structured in long span bridges, without a stiffener. Recently, it has been used in many countries in Europe, Canada, Japan, and the US with the help of international committees such as CIDECT(International Committee for the Development and Study of Tubular Structures and International Institute of Welding). The most important problem when using the tubular member is the fact that it is difficult to test the fatigue stress determined by nominal stress, since geometrical stress concentration occurs due to the welded joint's nod of complexity. The purpose of this study is to compare and examine current theories and widely applied Hot Spot stress determinations through finite element analysis, which is about welded joints of steel tubular truss. We would like to suggest a way of design practice which involves a bridge plan with rarely domestically used steel tubular truss` basic research data as well as considering the future of tubular member.

• 한국지열·수열에너지학회논문집
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• 제6권2호
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• pp.9-14
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• 2010

The ground source heat pump system is increasingly being considered as an alternative to traditional heating and cooling systems to reduce the emission of ground house gases. In this paper, A series of numerical analysis for energy piles has been performed focusing on heat transfer efficiency, performance and thermal stress. Results of numerical analyses for the W-shape type shows more efficient heat exchange transfer than the coil type. From results of the thermo-mechanical analysis, it is shown that the concentration of thermal stress occurs around the circulating pipe and the interfaces between different materials. The largest deformation caused by thermal stress is observed in the energy pile.

• 대한기계학회논문집A
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• 제34권5호
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• pp.561-565
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• 2010

용접부는 일반적으로 잘 알려져 있는 바와 같이, 외력에 의한 응력 집중원(stress concentration source)이 되는 것은 물론 용접과정에서의 입열(heat input)로 인한 성분과 조직의 변화에 의해 반복하중에 대한 피로강도(fatigue strength)가 모재의 그것에 비해 훨씬 떨어져서 피로균열(fatigue crack)의 발단이 되고 있다. 따라서, 본 연구에서는 실제로 철도차량에 적용되는 가스용접 이음재 가운데 대표적인 형상시편들을 재질별로 피로실험을 수행하여 피로하중범위-피로수명(${\Delta}P-N_f$) 관계를 도출하여 비교 평가하였다. 또한, 수치 해석적 유한요소법을 이용하여 용접이음재의 응력분포를 해석한 후, 용접이음부에 발생하는 최대주응력으로 (${\Delta}P-N_f$) 관계를 $\Delta\sigma-N_f$ 관계로 재정리하였다. 이상의 결과들을 바탕으로, 가스용접이 적용된 철도차량 차체의 경제적이고 합리적인 피로설계를 위한 기초정보로 사용하고자 하였다.

• Korea Journal of Cosmetic Science
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• 제1권1호
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• pp.1-9
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• 2019

The identification of compounds with anti-senescence activity in cell culture system is a first step in aging research. Given that pyruvate can be used energy source by conversion to acetyl-CoA in mitochondria, and protects cultured cell from various stress-induced cell damage and cell death, synthetic media (e.g., DMEM) often includes 1 mM pyruvate, which is very higher than the pyruvate concentration in human blood (approximately 30 ��M). However, the use of medium containing high concentration of pyruvate is not suitable for screening anti-senescence compounds, because pyruvate also protects against the cellular senescence of primary human dermal fibroblasts (NHDFs) through NAD+ generated during conversion to lactate. In this study, four extracts, i.e., Sprouted seed and fruit complex, Poncirus trifoliata fruit extract, Jaum balancing complex, and Prunus mume extract were used for evaluation of different anti-senescence effect in the absence or presence of 0.1 mM pyruvate, similar to the physiological pyruvate concentration. The senescence in NHDFs cultured with DMEM in the presence of 0.1 mM pyruvate (approximately the physiological concentration in human blood) is accelerated, as observed in pyruvate deprivation conditions. The cytotoxicity of the Poncirus trifoliata fruit extract was protected by pyruvate, and Jaum balancing complex and Prunus mume extract had anti-senescence activity in the presence of 0.1 mM pyruvate, but not in the absence of pyruvate. Given that pyruvate is a powerful protector against both cytotoxicity and cellular senescence, the screening of candidate agents for anti-senescence in high pyruvate conditions using an in vitro cell culture system is not valid. Therefore, we recommend the use of a low concentration of pyruvate to evaluate the anti-senescence effects of candidates, which is more similar to in vivo aging conditions than excessive stress-induced senescence models, to exclude the effect of excessive pyruvate in vitro.

• Journal of Mechanical Science and Technology
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• 제17권3호
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• pp.440-448
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• 2003

In the present paper, numerical simulations of buoyant plume dispersion in a neutral and stable atmospheric boundary layer have been carride out. A Lagrangian Stochastic Model (LSM) with a Non-Linear Eddy Viscosity Model (NLEVM) for turbulence is used to generate a Reynolds stress field as an input condition of dispersion simulation. A modified plume-rise equation is included in dispersion simulation in order to consider momentum effect in an initial stage of plume rise resulting in an improved prediction by comparing with the experimental data. The LSM is validated by comparing with the prediction of an Eulerian Dispersion Model (EDM) and by the measured results of vertical profiles of mean concentration in the downstream of an elevated source in an atmospheric boundary layer. The LSM predicts accurate results especially in the vicinity of the source where the EDM underestimates the peak concentration by 40% due to inherent limitations of gradient diffusion theory. As a verification study, the LSM simulation of buoyant plume dispersions under a neutral and stable atmospheric condition is compared with a wind-tunnel experiment, which shows good qualitative agreements.

• Journal of Welding and Joining
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• 제31권6호
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• pp.96-106
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• 2013

In this study, the effect of laser source($CO_2$ and Nd:YAG) on the microstructure and tensile properties of laser welded Al-Si coated boron steel(1.2mmt) was investigated with before and after hot-stamping. In case of as welds condition, fracture occurred in base metal unrelated to the laser source. It could be explained that tensile strength of fusion zone composed of martensite and bainite is higher than that of base metal that contains a lot of ferrite despite dilution of Al and Si from coating layer to fusion zone. In case of hot-stamping condition, the fracture occurred in fusion zone irrelevant to laser source and the tensile strength was lower than hot stamped base metal. In the $CO_2$ laser welds, $Fe_3$(Al,Si) formed near the bond line was transformed into ferrite during hot-stamping. Therefore tensile strength of bond line is lower than that of base metal and center of fusion zone and the fracture occurred in the bond line. On the other hand, in the Nd:YAG laser welds, the higher concentration of Al formed the ferrite in the fusion zone during hot-stamping treatment. Also, the thickness of centerline was thinner than that of base metal. Therefore, it is considered that fracture occurred in centerline of fusion zone due to effect of concentration stress, and it leaded to a lower tensile strength and elongation.

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 1997년도 제25회 춘계학술대회
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• pp.74-79
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• 1997

Most of the results of recent research for the influence of indentation on rolling contact fatigue has been carried out with high strength material under the point contact. The plastic lug around the indent also has been known as a source of stress concentration. This study is undertaken to analyze the influence of indentation on rolling contact fatigue with low strength material under the line contact. The results in this study show that the plastic flow around indentation by rolling friction has a major influence, differed from the results of high strength material. And the change of residual stress and half-value breadth measured to the failure by X-ray diffraction can be identified to predict the rolling contact fatigue life of indented materials.

• KSBB Journal
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• 제16권4호
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• pp.415-419
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• 2001

Bacillus subtilis WB700에서 P43 프로모터를 사용하여 staphylokinase를 생산하기 위하여 배지 최적화 및 회분식 배양과 유가식 배양 두가지 시스템을 비교하였다. 여러 가지 질소윈 중에서는 tryptone이 가장 좋은 질소원 이었으며, MSR 배지를 사용한 경우 tryptone 15 g/L일 경우에 최적조건임을 알아내었다. MSR 배지에시 포도당을 제한 기질로 사용할 경우는 5 g/L일 때가 SAK의 발현에 최적 조건이었다. MSR 배지를 기본으로 이용하여 포도당 공급을 조절함으로서 발효조 내의 DO를 30%로 유지한 결과 오히려 MSR 배지를 이용하여 회분식 발효를 한 경우보다 좋지 못한 결과를 얻었으며, 이는 B. subtilis 숙주의 영양요구적 특이성과 P43 promoter의 stress 발생시 주 발현되는 특성 등에 기인한 것이라고 사료된다. MSR 배지를 이용하여 회분식 발효를 하였을 때 SAK 활성은 2880 unit이었고, 이때 배지 내로 분비된 SAK 농도는 455 mg/L이었다.

• Journal of Animal Science and Technology
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• 제47권2호
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• pp.253-260
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• 2005

This study was conducted to utilize the wastes produced from thinning the forest as a roughage source for ruminants. Four ruminally cannulated Korean steers were used to investigate the ruminal fermentation characteristics and blood concentrations of stress-related hormones. Treatments were composed of the rice straw only (Control) and the 30% pine silage plus 70% rice straw(Pine silage) as roughages. The experiment was conducted with four replicates by a double tum over design. For sampling of blood under high temperature steers were accommodated in respiratory chamber. Concentrations of ruminal pH, volatile fatty acids and ammonia were not affected by substitution of pine silage. Mean concentration of blood thyroxine was significantly(P < 0.01) lower in the pine silage than the control. However, blood cortisol concentration was not affected by substitution of pine silage, even though it was significantly(P < 0.05) decreased after exposure for 6 hrs in high temperature. It was concluded that the pine silage using the wastes produced from thinning the forest could be use as a substitute roughage for reduction of heat stress in ruminant.

• KSBB Journal
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• 제9권2호
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• pp.140-146
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• 1994

A. pullulans로부터 polyol 생산 특성을 검토하기 위하여 탄소원, 수분활성의 영향을 검토하였다. 생산된 polyol 중에서 mannitol이 주성분이었고 소량의 glycerol이 함께 생산되었다. 검토된 탄소원 중 sucrose, glucose, fructose, mannose를 단일 탄소원으로 사용하였을 때 mannitol을 생산할 수 있었으며, 20% sucrose 배지에서 polyol 생산량이 최대였고 이때 대당 mannitol의 수율($Yp_{p/s}$)은 0.227이었다 두 종류의 유용한 탄소원인 glucose와 sucrose에 대한 초기기질농도에 대한 $Yp_{p/s}$는 거의 일정한 값을 보여, 일반적인 내삼투성 미생물과는 달리 첨가된 염(NaCl, KCI)의 농도가 증가할수록, 즉 수분활성이 낮은 조건에서는 오히려 mannitol의 생산량이 낮아졌으며, 상대적으로, glycerol의 양은 증가하였다 실험결과를 종합해 볼 때, A. pullulans에 의한 polyol 중, glycer이의 경우는 다른 내삼투성 미생물의 경우와 같이 생산되지만, mannitol의 경우는 sucrose, glucose 등의 유용한 탄소원들의 대사과정 중에 인산화경로(phosphate pathway)과 환원효소작용(enzymatic dehydrogenation)을 통해 생성되는 발효산물 이라는 결론을 얻었다.