• 제목/요약/키워드: The laboratory

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The recognition of development plan and what to pursue in dental laboratory industry (치과기공산업 발전 방안 및 추구해야 할 방안에 관한 인식도)

  • Kim, Kap-Jin
    • Journal of Technologic Dentistry
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    • v.39 no.2
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    • pp.119-128
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    • 2017
  • Purpose: In order to increase competitiveness for the growth and development of the dental laboratory industry, we plan to develop the dental laboratory industry. Methods: A total of 547 questionnaires were used as the final analysis data for the dental technicians from all over country participated in the 51st Korea Dental Technology Expo & Scientific Conference of the Korean Dental Technologist Association held in KINTEX from July 18 to 19, 2015. The questionnaire items consisted of 28 items in terms of general characteristics, questions about the methods to be pursued for the development of the dental laboratory industry, and recognition about the methods to be pursued to develop the dental laboratory industry. The collected data were analyzed by SPSS Ver. 21.0 for windows. Results: To improve the dental laboratory industry, actualization of dental laboratory products fee(41.2%) had the highest, followed by improving treatment of dental technician, standardization of dental laboratory products, direct bill of medical insurance, regulation of contract management on huge capital, and etc. The recognition of the measures to be pursued for the development of the dental laboratory industry was that dental laboratory products fee required to receive more than 20% of the dental prosthesis fee highest($4.62{\pm}0.76$). And to enlarge dental laboratories through M&A between dental laboratories is the lowest($3.39{\pm}1.26$). Conclusion: As a means to pursue the development of the dental laboratory industry, the actualization of dental laboratory products fee was proposed. Recognition also showed that dental laboratory products fee required to receive more than 20% of dental prosthesis fee was the highest. It is important to propose a reasonable dental laboratory products fee because it recognizes that it is necessary to promote economic growth in both development plan and awareness.

Three Month Subacute Toxicity Study of Ginkgo Biloba Extract(EGb 761) in Rats

  • Lee, Yong-Soon;Nam, Jeong-Seok;Che, Jeong-Hwan;Lee, Suk-Man;Yang, Jae-Man;Kang, Byeong-Cheol;Lee, Hak-Mo;Park, Jae-Hak;Chai, Chan-Hee;Kang, Sung-An
    • Toxicological Research
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    • v.12 no.1
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    • pp.113-119
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    • 1996
  • Group of 40 male and 40 female Sprague-Dawley rats was given daily intravenous injections of different dosage of Ginkgo biloba extract(EGb 761), 7.5 mg/kg/day (low dosage group), 15 mg/kg/day (middle dosage group), or 30 mg/kg/day (high dosage group)for 3 month by tail vein according to Established Regulation of Korean National Institute of Safety Research (1994. 4. 14). Appearance, behavior, mortality, and food consumption of rats of treated groups were not affected during the experimental periods. No significant Ginkgo biloba extract(EGb 761)-related changes were found in urinalysis, hematology, serum chemistry, and organ weight. No histopathological lesions were seen in both control and treatment groups. Our results strongly suggest that no toxic changes were found in rat treated intravenously with Ginkgo biloba extract(EGb 761)for 3 month.

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Factors Affecting the Job Performance of Clinical Laboratory Scientists

  • Shim, Moon-Jung
    • Korean Journal of Clinical Laboratory Science
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    • v.41 no.3
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    • pp.140-144
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    • 2009
  • Clinical laboratory testing plays a crucial role in the detection, diagnosis, and treatment of disease. Clinical laboratory scientists evaluate test results, develop and modify procedures, and establish and monitor programs, to ensure the accuracy of tests. It is clear that over the past decade the role of the clinical laboratory scientists has expanded and this process will be continued into the future. The purpose of this study is to clarify the factors influencing the job performance of clinical laboratory scientists and aim to provide the role and duties of clinical laboratory scientists. To examine the frequency of work context and the importance of task, the surveys were conducted on 168 clinical laboratory scientists. The questionnaire items were used 10 score by Likert scale. According to the results of this study, the important factor affecting the job performance was "Analyze laboratory findings to check the accuracy of the results", and the next factors were "Establish and monitor quality assurance programs", "Enter data from analyzer into computer", "Calibrate and maintain equipment". And the factors of "repeating same tasks" and "spend time making repetitive motions" were answered the highest. This study will provide strategies for improving work environment, developing education curriculum and method, and role of clinical laboratory scientists. We must continually upgrade our knowledge, and identify the new trends in technology and science and accept changes.

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Codon Optimization, Soluble Expression and Purification of PE_PGRS45 Gene from Mycobacterium tuberculosis and Preparation of Its Polyclonal Antibody Protein

  • Xu, Tao;Li, Minying;Wang, Chutong;Yuan, Meili;Chang, Xianyou;Qian, Zhongqing;Li, Baiqing;Sun, Meiqun;Wang, Hongtao
    • Journal of Microbiology and Biotechnology
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    • v.31 no.11
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    • pp.1583-1590
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    • 2021
  • Studies have demonstrated that PE_PGRS45 is constitutively expressed under various environmental conditions (such as nutrient depletion, hypoxia, and low pH) of the in vitro growth conditions examined, indicating that PE_PGRS45 protein is critical to the basic functions of Mycobacterium tuberculosis. However, there are few reports about the biochemical function and pathogenic mechanism of PE_PGRS45 protein. The fact that this M. tuberculosis gene is not easily expressed in E. coli may be mainly due to the high content of G+C and the use of unique codons. Fusion tags are indispensable tools used to improve the soluble expression of recombinant proteins and accelerate the characterization of protein structure and function. In the present study, His6, Trx, and His6-MBP were used as fusion tags, but only MBP-PE_PGRS45 was expressed solubly. The purification using His6-MBP tag-specific binding to the Ni column was easy to separate after the tag cleavage. We used the purified PE_PGRS45 to immunize New Zealand rabbits and obtained anti-PE_PGRS45 serum. We found that the titer of polyclonal antibodies against PE_PGR45 was higher than 1:256000. The result shows that purified PE_PGRS45 can induce New Zealand rabbits to produce high-titer antibodies. In conclusion, the recombinant protein PE_PGRS45 was successfully expressed in E. coli and specific antiserum was prepared, which will be followed by further evaluation of these specific antigens to develop highly sensitive and specific diagnostic tests for tuberculosis.

Reclassification of Xanthomonas Isolates Causing Bacterial Leaf Spot of Euphorbia pulcherrima

  • Li, Bin;Yu, Rongrong;Shi, Yu;Su, Ting;Wang, Fang;Ibrahim, Muhammad;Xie, Guanlin;Wang, Yanli;Sun, Guochang
    • The Plant Pathology Journal
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    • v.27 no.4
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    • pp.360-366
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    • 2011
  • Bacterial leaf spot of Euphorbia pulcherrima has been reported in many countries. Characterization by polyphasic approaches indicated that the isolates from India, USA and New Zealand could be distinguished based on rep-PCR profiles and gyrB phylogenies, while the Chinese isolates should be ascribed to Xanthomonas axonopodis pv. poinsettiicola.