• 동의생리병리학회지
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• 제22권6호
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• pp.1462-1469
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• 2008

The purpose of the study was to confirm what effect HRHDT treatment had on cell extinction by damage of endoplasmic reticulum induced to PC-12 cell damage by glucose deprivation. The study confirmed what effect it had on forming the condition of glucose deprivation within a culture fluid of PC-12 cell and on a nerve cell's survival rates and tested whether HRHDT could prevent extinction of PC-12 cell by glucose deprivation. Also, the study confirmed what effect HRHDT treatment had on the emitted quantity of LDH by glucose deprivation. To examine PC-12 cell's behavioral change under the condition of glucose deprivation and a protective effect of HRHDT on the change, the study observed PC-12 cell's behavioral change with a microscope. Also, the study confirmed density of calcium ion within cells followed by a culture time in the condition of glucose deprivation with FACS and confirmed what effect HRHDT treatment had on the above density of calcium ion within cells. Finally, the study carried out the western blot and confirmed what effect HRHDT treatment had on revelation of GRP 78 and CHOP protein and a segmental type of aspase 12. In this study, HRHDT rescued PC-12 cells from glucose deprivation-induced cell death. HRHDT also prevents the LDH release, Ca++ accumulation, and morphological change, which was associated with the ER stress. Furthermore, HRHDT reduced the expression of ER chaperone (Grp78 and CHOP) proteins by glucose deprivation in PC-12 cells. These results suggest that HRHDT might provide a useful therapeutic strategy in treatment of the neurodegenerative diseases caused by glucose deprivation injuries.

• 대한물리치료과학회지
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• 제29권4호
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• pp.65-74
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• 2022

Background: This study attempted to find out the degree of awareness of owners who have the most influence on companion animal's rehabilitation exercise, and the purpose of the treatment effect, and the expectations of rebilitation treatment. The purpose of this study is to find out about the awareness, comprehension, and expectations of caregiver for rehabilitation exercise in companion animal medical services, and to develop them into a field of animal medical services. Design: The tool used in this study used a questionnaire, and the composition consists of the subject's general characteristics, recognition of companion animal rehabilitation, comprehension and expectation. Methods: A questionnaire was given to 103 companion animal's owners, and all were collected and analyzed. Analysis of the frequency analysis method, Fisher's exact test were analyzed. Results: It was confirmed that the difference was significant in the question of whether the caregivers had heard companion animal rehabilitation (p<.05). When asked that rehabilitation is necessary to improve physical function, the difference was confirmed to be significant (p<.05). The significance was confirmed when asked if the caregiver was willing to participate in animal rehabilitation education (p<.05). When asked if caregiver was willing to recommend rehabilitation exercise for companion animal of other caregiver who need rehabilitation exercise, it was confirmed that the difference was significant (p<.05). Conclusion: There was significant difference between gender. This study confirmed that the definite concept caregivers' perception in animal rehabilitation was not established. Therefore, in order to improve perception of caregivers, interdisciplinary exchanges and publicity for animal rehabilitation are needed, and the development of training and education programs for rehabilitation experts was suggested.

• 열처리공학회지
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• 제37권3호
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• pp.114-120
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• 2024

AA 2026, which is used as an aviation material, is an improved version of 2024 and has higher physical properties, and is a material that has the potential to be applied to supersonic aircraft to be developed in the future. However, when an aircraft exceeds supersonic speeds, the surface heats up and the material must be resistant to this. Therefore, this study confirmed the high-temperature properties of AA 2026, an aviation structural material. AA 2026, solution treated at 500℃ for 4hr, was naturally aged at room temperature for more than 168 hr. Changes in microstructure and physical properties were confirmed over several hours of exposure to 100℃, 200℃, and 300℃, respectively. As a result of microstructure analysis, there was no significant change at 100℃, and from 200℃, GPB, a strengthening mechanism, grew and formed an S Phase. It was confirmed that the S Phase grew as the exposure time increased. Through a tensile test, it was confirmed that physical properties deteriorated as the precipitates grew. However, it was confirmed that the properties were stably maintained at 100℃, which is the temperature when the speed of a supersonic aircraft is less than Mach 2.

• 정신신체의학
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• 제4권2호
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• pp.198-206
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• 1996

To assess premenstrual changes in adolescents, a self rating retrospective premenstrual assessment from(PAF) and a prospective daily rating form(DRF) were administered to 230 high school students who reported premenstrual changes. The results were as follows 1) According to typological categories of retrospective premenstrual assessment form, the most commonly reported changes in mood and behavior were major depressive syndrome(40.0%), impulsive syndrome(20.9%). Especially among the subtype of depressive syndrome, hostility feature and withdrawal feature were most frequent. The most commonly reported changes in physical condition were general discomfort(73.5%), fatigue(33.9%). 50.9% of all subjects reported impaired social function. 2) 41.2% of the subjects who met the criteria for typological categories of retrospective premenstrual assessment form were confirmed by prospective daily rating form. 3) According to different criteria of percent change method of assessing symptom change, 38.7% of all subjects had at least one mild premenstrual change and 23.5% had at least one moderate premenstrual change. 4) Using prospective daily rating form, overall psychological changes were confirmed more frequently than physical changes. Of the mild premenstrual changes, 'low energy, tired & weak', 'less or impaired work' were most common changes. Of the moderate premenstrual changes, the most common change was 'low energy, tired & weak'. 5) As attitude toward menstruation, 44.9% of the subjects with confirmed premenstrual changes had more negative attitude(disgusted) toward menstruation. 6) The subjects with confirmed mild or moderate premenstrual changes had only dysphoric mood changes or both physical and dysphoric mood changes, but did not have only physical changes. These results suggest that retrospective reports of premenstrual changes were partially confirmed by daily prospective ratings. A substantial number of adolescents appeared to experience premenstrual changes, and the most common confimed premenstrual change of mild or moderate severity was 'low energy, tired and weak'.

• Asian-Australasian Journal of Animal Sciences
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• 제32권3호
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• pp.350-356
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• 2019

Objective: To examine the regulatory effects of exercise on myokine expression in horse skeletal muscle cells, we compared the expression of several myokine genes (interleukin 6 [IL-6], IL-8, chemokine [C-X-C motif] ligand 2 [CXCL2], and chemokine [C-C motif] ligand 4 [CCL4]) after a single bout of exercise in horses. Furthermore, to establish in vitro systems for the validation of exercise effects, we cultured horse skeletal muscle cells and confirmed the expression of these genes after treatment with hydrogen peroxide. Methods: The mRNA expression of IL-6, IL-8, CXCL2, and CCL4 after exercise in skeletal muscle tissue was confirmed using quantitative-reverse transcriptase polymerase chain reactions (qRT-PCR). We then extracted horse muscle cells from the skeletal muscle tissue of a neonatal Thoroughbred. Myokine expression after hydrogen peroxide treatments was confirmed using qRT-PCR in horse skeletal muscle cells. Results: IL-6, IL-8, CXCL2, and CCL4 expression in Thoroughbred and Jeju horse skeletal muscles significantly increased after exercise. We stably maintained horse skeletal muscle cells in culture and confirmed the expression of the myogenic marker, myoblast determination protein (MyoD). Moreover, myokine expression was validated using hydrogen peroxide ($H_2O_2$)-treated horse skeletal muscle cells. The patterns of myokine expression in muscle cells were found to be similar to those observed in skeletal muscle tissue. Conclusion: We confirmed that several myokines involved in inflammation were induced by exercise in horse skeletal muscle tissue. In addition, we successfully cultured horse skeletal muscle cells and established an in vitro system to validate associated gene expression and function. This study will provide a valuable system for studying the function of exercise-related genes in the future.

• Fisheries and Aquatic Sciences
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• 제26권2호
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• pp.97-104
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• 2023

CD96 is a membrane-bound receptor discovered in humans in 1992 that is mainly present in natural killer cells and T cells derived from haematopoietic cells and performs immune functions. Based on the sequence of CD96 obtained from red seabream (Pagrus major), phylogenetic analysis with other species, infections of normal fish, Streptococcus iniae and red sea bream iridovirus (RSIV), and expression analysis was conducted using real-time polymerase chain reaction. Phylogenetic analysis showed the highest homology with Sparus aurata, and multiple sequence analysis confirmed the conservation of major domains between different fish species. Normal fish high expression results were confirmed in the head kidney, and spleen, which are the haematopoietic organs of the fish. High expression levels were confirmed in the gills, liver, spleen, and kidney on day three after RSIV infection. After S. iniae infection, high expression was confirmed in the gills and liver on day one, and high expression was confirmed in the spleen from 12 hours. These results show that PmCD96 functions as an immune gene in P. major and is considered a basic research case for CD96 in fish's hematopoietic organ immune system.

• Journal of Plant Biotechnology
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• 제49권3호
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• pp.250-256
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• 2022

This study was conducted to measure changes in polyphenol components and antioxidant effects of Cirsium Lineare (Thunb.) after fermentation by lactic acid bacteria. First, Cirsium Lineare (Thunb.) extract (CE, unfermented) and Cirsium Lineare (Thunb.) extract fermented with Lactobacillus paracasei (FCE) were prepared. Changes in components resulting from fermentation were confirmed through changes in polyphenol compound content and silymarin derivative pattern, and antioxidant activity was confirmed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, and ferric reducing antioxidant power (FRAP) analyses. As a result, polyphenol contents of CE and FCE were confirmed as 21.94 ± 1.15 and 67.90 ± 4.48 mg GAE/g, respectively. Both values were increased approximately three times by fermentation, and there was also a change in the silymarin derivative pattern. In the case of DPPH radical RC₅₀ values in particular, CE and FCE were confirmed to inhibit DPPH radicals by 50% at concentrations of 129.44 ± 5.85 and 50.00 ± 3.47 ㎍/mL, respectively, with the FCE value approximately 2.5 times lower than that of CE. In addition, ABTS radical scavenging and FRAP activity were confirmed to share similar trends as DPPH radical scavenging activity. When CE and FCE were compared, FCE showed a better antioxidant effect overall. In conclusion, this study suggested that FCE prepared through lactic acid bacteria fermentation may be utilized as a powerful antioxidant material.

• 한국농공학회지
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• 제26권3호
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• pp.90-98
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• 1984

In this paper, the anthor made a basic study of the storage function model and examined several constants in applying the storage function model to flood run-off analysis by dealing with the data in the Supyung and Hoyng Syung watershed, the applicabilities of the storage function model are examined by searching this optimum model parameters in two watersheds. The results are summarized as follows, 1) The optimum values of the exponential constants, P, in the storage function model showed to be 0.77 to 0.87 in two watersheds observed, therefore it was confirmed that the storage fumction model was approaching to the surface runoff model. 2) It was confirmed that the interval of variation of the storage constant, K, Showed to be larger than that of the exponential constant, p. 3) Relative erros in the discharge obtained by using the storage function model and the SDFP mothod showed to be 20 and 17 percent respectively to the observed discharge, therefore it was confirmed that the applicability of the storage function model using the SDFP method are excellent for runoff analysis. 4) A simple method is proposed for estimating the lag time in the storage function model.

• International journal of advanced smart convergence
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• 제11권2호
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• pp.142-152
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• 2022

We study confirmed the relationship between the adversity index, entrepreneurial orientation, and organizational learning competency perceived by organizational members as follows. First, the adversity index showed a positive (+) effect on entrepreneurial orientation (hypothesis 1) and organizational learning competency (hypothesis 2). Second, the entrepreneurial orientation was statistically significant in organizational learning competency (hypothesis 3). Third, the partial mediating role of entrepreneurial orientation (Hypothesis 4) was confirmed in the process of the adversity index affecting organizational learning competency. Meanwhile, the main implications of this study are as follows. First, it is the aspect that provides additional theoretical implications in the reality that studies on the adversity index and entrepreneurial orientation that affect organizational learning competency are lacking. Second, it is the aspect that the importance of adversity index and start-up orientation was confirmed in improving organizational learning competency based on securing differentiated competitiveness for the advancement of the organization's sustainability management system. In addition, it is the aspect of drawing practical implications for strategic human resource management and human resource development to systematically improve it.

• 한국농공학회지
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• 제39권1호
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• pp.93-101
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• 1997

This paper presents yield surfaces and hardening laws for describing the state of an unsaturated soil under isotropic compression and suction changes. The yield surface is formulated within the framework of hardening plasticity using two independent sets of stress variables : the excess of total stress over air pressure and the suction. And the application of the yield surfaces and hardening laws are confirmed from the result of the experiment. To this end a series of suction-controlled isotropic tests are conducted on clayey soils. Matric suction is controlled by the axis translation technique using high air entry ceramic disk. The specimens are compacted using a half of Proctor compaction energy with 5 % lower of water content than the optimum moisture contents. From test results, existence of the yield surfaces and an application of hardening laws to samples are confirmed by comparison between test and predicted results. And it is confirmed that LC yield locus is extened with the total plastic deformations induced by suction or stress changes, however, SI yield locus is only extended with the plastic deformations by induced suction changes.