• The Transactions of The Korean Institute of Electrical Engineers
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• v.59 no.11
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• pp.1971-1979
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• 2010

As a result of increasing environmental concern, the penetration of renewable power on power systems is now increasing. Wind energy can be considered as the most economical energy sources to generate electricity without depletion of fossil fuel. To devise adequate control strategies for wind farm, time domain simulation analysis needs to be performed. This presents a continuation time integration (CTI)-based time domain simulation algorithm for wind farm with doubly fed asynchronous generator (DFAG) connected to the external power systems. This paper depicts how to time trajectories are traced using CTI-based time domain simulation. Also this paper describes the possibilities of hierachical control for wind farm output limitation, and the coordinated control has been designed by hierarchical control structured from central control level to wind farm control board and to an individual wind turbine level. Finally it shows an illustrative example of time domain simulation result with two test systems through case study.

• Structural Engineering and Mechanics
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• v.78 no.3
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• pp.369-378
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• 2021

This paper reviewed a few output-only system identification algorithms and identified the shortcomings of those popular blind source separation methods. To address the issues such as less sensors than the targeted modal modes (under-determinate problem), repeated natural frequencies as well as systems with complex mode shapes, this paper proposed a complex wavelet modified second order blind identification method (CWMSOBI) by transforming the time domain problem into time-frequency domain. The wavelet coefficients with different dominant frequencies can be used to address the under-determinate problem, while complex mode shapes are addressed by introducing the complex wavelet transformation. Numerical simulations with both high and low signal-to-noise ratios validate that CWMSOBI can overcome the above-mentioned issues while obtaining more accurate identified results than other blind identification methods.

• Wind and Structures
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• v.37 no.4
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• pp.255-274
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• 2023

The aim of this paper is to enhance the accuracy of predicting time-averaged external surface pressures on low-rise buildings by utilizing Computational Fluid Dynamics (CFD) simulations. To achieve this, benchmark studies of the Silsoe cube and the Texas Tech University (TTU) experimental building are employed for comparison with simulation results. The paper is structured into three main sections. In the initial part, an appropriate domain size is selected based on the precision of mean pressure coefficients on the windward face of the cube, utilizing Reynolds Averaged Navier-Stokes (RANS) turbulence models. Subsequently, recommendations regarding the optimal computational domain size for an isolated building are provided based on revised findings. Moving on to the second part, the Silsoe cube model is examined within a horizontally homogeneous computational domain using more accurate turbulence models, such as Large Eddy Simulation (LES) and hybrid RANS-LES models. For computational efficiency, transient simulation settings are employed, building upon previous studies by the authors at the Windstorm Impact, Science, and Engineering (WISE) Lab, Louisiana State University (LSU). An optimal meshing strategy is determined for LES based on a grid convergence study. Three hybrid RANS-LES cases are investigated to achieve desired enhancements in the distribution of mean pressure coefficients on the Silsoe cube. In the final part, a 1:10 scale model of the TTU building is studied, incorporating the insights gained from the second part. The generated flow characteristics, including vertical profiles of mean velocity, turbulence intensity, and velocity spectra (small and large eddies), exhibit good agreement with full-scale (TTU) measurements. The results indicate promising roof pressures achieved through the careful consideration of meshing strategy, time step, domain size, inflow turbulence, near-wall treatment, and turbulence models. Moreover, this paper demonstrates an improvement in mean roof pressures compared to other state-of-the-art studies, thus highlighting the significance of CFD simulations in building aerodynamics.

• Journal of the Korean GEO-environmental Society
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• v.15 no.5
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• pp.31-37
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• 2014

In situ investigations and laboratory tests using elastic wave have become popular in geotechnical and geoenvironmental engineering. Propagation velocity of elastic wave is the key index to evaluate the ground characteristics. To evaluate this, various methods were used in both time domain and frequency domain. In time domain, the travel time can be found from the two points that have the same phase such as peaks or first rises. Cross-correlation can also be used in time domain by evaluating the time shift amount that makes the product of signals of input and received waveforms maximum. In frequency domain, wave propagation velocity can be evaluated by computing the phase differences between the source and received waves. In this study, wave propagation velocity evaluated by the methods listed above were compared. Bender element tests were conducted on the specimens cut from the undisturbed hand-cut block samples obtained from Block 37 excavation site in Chicago, IL, US. The evaluation methods in time domain provides relatively wide range of wave propagation velocities due to the noise in signals and the sampling frequency of data logger. Frequency domain approach provides relatively accurate wave propagation velocities and is irrelevant to the sampling frequency of data logger.

• Economic and Environmental Geology
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• v.43 no.4
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• pp.349-358
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• 2010

For imaging complex subsurface structures such as salt dome, faults, thrust belt, and folds, seismic prestack reverse-time migration in depth domain is widely used, which is performed by the cross-correlation of shot-domain wavefield extrapolation with receiver-domain wavefield extrapolation. We apply the prestack reverse-time migration, which had been developed at KIGAM, to the seismic field data set of Block 5 in Jeju basin of Korea continental shelf in order to improve subsurface syncline stratigraphy image of the deep structures under the shot point 8km at the surface. We performed basic data processing for improving S/N ratio in the shot gathers, and constructed a velocity model from stack velocity which was calculated by the iterative velocity spectrum. The syncline structure of the stack image appears as disconnected interfaces due to the diffractions, but the result of the prestack migration shows that the syncline image is improved as seismic energy is concentrated on the geological interfaces.

• Molecules and Cells
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• v.45 no.8
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• pp.564-574
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• 2022

Epithin/PRSS14 is a membrane serine protease that plays a key role in tumor progression. The protease exists on the cell surface until its ectodomain shedding, which releases most of the extracellular domain. Previously, we showed that the remaining portion on the membrane undergoes intramembrane proteolysis, which results in the liberation of the intracellular domain and the intracellular domain-mediated gene expression. In this study, we investigated how the intramembrane proteolysis for the nuclear function is initiated. We observed that ectodomain shedding of epithin/PRSS14 in mouse breast cancer 4T1 cells increased depending on environmental conditions and was positively correlated with invasiveness of the cells and their proinvasive cytokine production. We identified selenite as an environmental factor that can induce ectodomain shedding of the protease and increase C-C motif chemokine ligand 2 (CCL2) secretion in an epithin/PRSS14-dependent manner. Additionally, by demonstrating that the expression of the intracellular domain of epithin/PRSS14 is sufficient to induce CCL2 secretion, we established that epithin/PRSS14-dependent shedding and its subsequent intramembrane proteolysis are responsible for the metastatic conversion of 4T1 cells under these conditions. Consequently, we propose that epithin/PRSS14 can act as an environment-sensing receptor that promotes cancer metastasis by liberating the intracellular domain bearing transcriptional activity under conditions promoting ectodomain shedding.

• Journal of Microbiology and Biotechnology
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• v.28 no.8
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• pp.1401-1411
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• 2018

The serine-threonine kinase AKT plays a pivotal role in tumor progression and is frequently overactivated in cancer cells; this protein is therefore a critical therapeutic target for cancer intervention. We aimed to identify small molecule inhibitors of the pleckstrin homology (PH) domain of AKT to disrupt binding of phosphatidylinositol-3,4,5-trisphosphate (PIP3), thereby downregulating AKT activity. Liposome pulldown assays coupled with fluorescence spectrometry were used to screen flavonoids for inhibition of the AKT PH-PIP3 interaction. Western blotting was used to determine the effects of the inhibitors on AKT activation in cancer cells, and in silico docking was used for structural analysis and optimization of inhibitor structure. Several flavonoids showing up to 50% inhibition of the AKT PH-PIP3 interaction decreased the level of AKT activation at the cellular level. In addition, the modified flavonoid showed increased inhibitory effects and the approach would be applied to develop anticancer drug candidates. In this study, we provide a rationale for targeting the lipid-binding domain of AKT, rather than the catalytic kinase domain, in anticancer drug development.

• Environmental Mutagens and Carcinogens
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• v.22 no.3
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• pp.175-182
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• 2002

Endocrine disruptors (EDs) are the chemicals that affect endocrine systems through activation or inhibition of steroid hormone response. It is necessary to have a good system to evaluate rapidly and accurately endocrine-disrupting activities of suspected chemicals and their degradation products. The key targets of EDs are nuclear hormone receptors, which bind to steroid hormones and regulate their gene transcription. We constructed a co-expression system of Gal4p DNA binding domain (DBD)- ligand binding domain of human estrogen receptor $\alpha$ or $\beta$, and Gal4p transactivation domain (TAD)-co-activator AIB-1, SRC-1 or TIF-2 in Saccharomyces cerevisiae with a chromosome-integrated lacZ reporter gene under the control of CYC1 promoter and Gal4p binding site (GAL4 upstream activating sequence, GAL4$_{UAS}$). Expression of this reporter gene was dependent on the presence of estrogen or EDs in the culture medium. We found that the two-hybrid system with combination of the hER$\beta$ LBD and co-activator SRC-1 was most effective in the xenoestrogen-dependent induction of reporter activity. The extent of transcriptional activation by those chemicals correlated with their estrogenic activities measured by other assay systems, indicating that this assay system is efficient and reliable for measuring estrogenic activity. The data in this research demonstrated that the yeast detection system using steroid hormone receptor and co-activator is a useful tool for identifying chemicals that interact with steroid receptors.s.

• 한국신재생에너지학회:학술대회논문집
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• 2009.11a
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• pp.439-442
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• 2009

As a result of increasing environmental concern, the penetration of renewable power on power systems is now increasing. Wind energy can be considered as the most economical energy sources to generate electricity without depletion of fossil fuel. The penetration of wind energy from wind farm is getting larger and larger, so we need adequate control strategies for wind farm. To devise adequate control strategies for wind farm, time domain simulation analysis needs to be performed. This presents a Simultaneous Implicit-based time domain simulation algorithm for wind farm with DFAG (Doubly Fed Asynchronous generator) connected to the external power systems. This paper shows an illustrative example with a 5-bus test system.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.26 no.4
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• pp.301-308
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• 2013

In this paper, an accuracy analysis of parallel method based on non-overlapping domain decomposition method is carried out. In this approach, proposed by Tak et al.(2013), the decomposed subdomains do not overlap each other and the connection between adjacent subdomains is determined via simple connective finite element named interfacial element. This approach has two main advantages. The first is that a direct method such as gauss elimination is available even in a singular problem because the singular stiffness matrix from floating domain can be converted to invertible matrix by assembling the interfacial element. The second is that computational time and storage can be reduced in comparison with the traditional finite element tearing and interconnect(FETI) method. The accuracy of analysis using proposed method, on the other hand, is inclined to decrease at cross points on which more than three subdomains are interconnected. Thus, in this paper, an accuracy analysis for a novel non-overlapping domain decomposition method with a variety of subdomain numbers which are interconnected at cross point is carried out. The cause of accuracy degradation is also analyze and establishment of countermeasure is discussed.