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Development of Gene Based STS Markers in Wheat

  • Lee, Sang-Kyu;Heo, Hwa-Young;Kwon, Young-Up;Lee, Byung-Moo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.1
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    • pp.71-77
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    • 2012
  • The objective of this study is to develop the gene based sequence tagged site (STS) markers in wheat. The euchromatin enriched genomic library was constructed and the STS primer sets were designed using gene based DNA sequence. The euchromatin enriched genomic (EEG) DNA library in wheat was constructed using the $Mcr$A and $Mcr$BC system in $DH5{\alpha}$ cell. The 2,166 EEG colonies have been constructed by methylated DNA exclusion. Among the colonies, 606 colonies with the size between 400 and 1200 bp of PCR products were selected for sequencing. In order to develop the gene based STS primers, blast analysis comparing between wheat genetic information and rice genome sequence was employed. The 227 STS primers mainly matched on $Triticum$ $aestivum$ (hexaploid), $Triticum$ $turgidum$ (tetraploid), $Aegilops$ (diploid), and other plants. The polymorphisms were detected in PCR products after digestion with restriction enzymes. The eight STS markers that showed 32 polymorphisms in twelve wheat genotypes were developed using 227 STS primers. The STS primers analysis will be useful for generation of informative molecular markers in wheat. Development of gene based STS marker is to identify the genetic function through cloning of target gene and find the new allele of target trait.

Developments in Fire Sprinkler Technology

  • Rice, Douglas
    • Proceedings of the Korea Institute of Fire Science and Engineering Conference
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    • 1997.11a
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    • pp.606-609
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    • 1997
  • Technology in fire sprinklers has exploded. There is more private fire sprinkler research now than ever before. New technology in fire sprinklers has tested the existing standard and found that it was wrong in some cases, it has provided new sprinklers that control fire better while reducing the cost of the overall system. This expansion of the types and applications of fire sprinklers has created a great need for education for the Authorities Having Jurisdiction and the Engineer as they must be able to make informed decisions regarding this new technology. Significant research has been done at Factory Mutual Research Corporation and Underwriters Laboratory in the United States. Full scale fire testing is done to prove any new technology did not lower the level of protection of existing fire sprinkler system installations. In each and every case, the existing level of fire protection was the baseline against which the new sprinklers were tested. This level can not be lowered for the test to be considered a success. This is intended to be an overview of the developments in fire sprinklers. Although extensive detail is not included, the overall aspects of these developments can be discussed. The research that will be covered will include the following general subjects: high piled and rack storage sprinkler protection without in-rack sprinklers, extended coverage and quick response sprinklers for ordinary and light hazard occupancies, as well as special sprinklers and their applications.

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Interleaving Delay Diversity with Antenna Selection or the Multi-code WCDMA System (다중 코드 WCDMA 시스템에서 안테나 선택 기법을 적용한 인터리빙 딜레이 다이버시티 연구)

  • 정숙현;김낙명
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.6A
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    • pp.600-606
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    • 2004
  • An interleaving delay diversity scheme is investigated in this paperand the applications in the multi-code WCDMA system along with antenna selection diversity is proposed. The proposed algorithm utilizes interleaving and delayed duplicated transmission mechanism to mitigate the effect of noise and fading. The interleaved signal and the original signal are both transmitted at intervals to obtain time diversity without any increase in required capacity. By adjusting the number of retransmissions of the information signal properly, the system achieve the receive diversity efficiently. For transmission power efficiency the proposed system also applies antenna selection diversity. By computer simulation, it has been shown that the proposed algorithm achieves better performance than the conventional algorithms by more than 2dB.

Analysis of Academic Research Trends Pertaining to Globalization of Korean Food (한식의 세계화 관련 학술적 연구 동향 분석)

  • Min, Kye-Hong
    • Korean journal of food and cookery science
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    • v.25 no.5
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    • pp.606-618
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    • 2009
  • The purpose of this study was to analyze research trends in scholarly journals that were related to the globalization of Korean food and to provide information on the future of these trends such as various creative subject selection and research methods. 70 papers pertaining to Korean food research, such as academic journals expected to be registered, and national-scale academic papers, were selected. This research was conducted from April 6 to April 22. 2009. The results of this study were as the follows: First, most of the papers, 20(28.6%), were published in the journal of Korean journal of food culture. In addition, most articles had a single author, 24(34.3%). In regard to the profession of the auther, university professor was the most frequent profession, 127 persons(68.3%). In terms of the research subjects, preference was the most dominant preference(19.2%). Investigatory research was the most frequent research method, 58 papers(82.9%). Furthermore, foreigners and natives were the most dominant research objects, 21 papers(30.0%). As for the analysis methods, frequency analysis, one-way ANOVA, T-test, reliability analysis, and factor analysis were used the most in that order. Future studies should includ papers for master's degrees and doctorial degrees.

Path following of a surface ship sailing in restricted waters under wind effect using robust H guaranteed cost control

  • Wang, Jian-qin;Zou, Zao-jian;Wang, Tao
    • International Journal of Naval Architecture and Ocean Engineering
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    • v.11 no.1
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    • pp.606-623
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    • 2019
  • The path following problem of a ship sailing in restricted waters under wind effect is investigated based on Robust $H_{\infty}$ Guaranteed Cost Control (RHGCC). To design the controller, the ship maneuvering motion is modeled as a linear uncertain system with norm-bounded time-varying parametric uncertainty. To counteract the bank and wind effects, the integral of path error is augmented to the original system. Based on the extended linear uncertain system, sufficient conditions for existence of the RHGCC are given. To obtain an optimal robust $H_{\infty}$ guaranteed cost control law, a convex optimization problem with Linear Matrix Inequality (LMI) constraints is formulated, which minimizes the guaranteed cost of the close-loop system and mitigates the effect of external disturbance on the performance output. Numerical simulations have confirmed the effectiveness and robustness of the proposed control strategy for the path following goal of a ship sailing in restricted waters under wind effect.

CFD Numerical Calcultion for a Cavity Matrix Combustor Applying Biogas (바이오가스 적용 캐비티 매트릭스 연소기 CFD 수치연산)

  • CHUN, YOUNG NAM;AN, JUNE
    • Journal of Hydrogen and New Energy
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    • v.33 no.5
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    • pp.598-606
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    • 2022
  • With the advancement of industry, the use of various sustainable energy sources and solutions to problems affecting the environment are being actively requested. From this point of view, it is intended to directly burn unused biogas to use it as energy and to solve environmental problems such as greenhouse gases. In this study, a new type of cavity matrix combustor capable of low-emission complete combustion without complex facilities such as separation or purification of biogas produced in small and medium-sized facilities was proposed, and CFD numerical calculation was performed to understand the performance characteristics of this combustor. The cavity matrix combustor consists of a burner with a rectangular porous microwave receptor at the center inside a 3D cavity that maintains a rectangular parallelepiped shape composed of a porous plate that can store heat in the combustor chamber. As a result of numerical calculation, the biogas supplied to the inlet of the combustor is converted to CO and H2, which are intermediate products, on the surface of the 3D matrix porous burner. And then the optimal combustion process was achieved through complete combustion into CO2 and H2O due to increased combustibility by receiving heat energy from the microwave heating receptor.

The N-terminal peptide of the main protease of SARS-CoV-2, targeting dimer interface, inhibits its proteolytic activity

  • Sunyu Song;Yeseul Kim;Kiwoong Kwak;Hyeonmin Lee;Hyunjae Park;Young Bong Kim;Hee-Jung Lee;Lin-Woo Kang
    • BMB Reports
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    • v.56 no.11
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    • pp.606-611
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    • 2023
  • The main protease (Mpro) of SARS-CoV-2 cleaves 11 sites of viral polypeptide chains and generates essential non-structural proteins for viral replication. Mpro is an important drug target against COVID-19. In this study, we developed a real-time fluorometric turn-on assay system to evaluate Mpro proteolytic activity for a substrate peptide between NSP4 and NSP5. It produced reproducible and reliable results suitable for HTS inhibitor assays. Thus far, most inhibitors against Mpro target the active site for substrate binding. Mpro exists as a dimer, which is essential for its activity. We investigated the potential of the Mpro dimer interface to act as a drug target. The dimer interface is formed of domain II and domain III of each protomer, in which N-terminal ten amino acids of the domain I are bound in the middle as a sandwich. The N-terminal part provides approximately 39% of the dimer interface between two protomers. In the real-time fluorometric turn-on assay system, peptides of the N-terminal ten amino acids, N10, can inhibit the Mpro activity. The dimer interface could be a prospective drug target against Mpro. The N-terminal sequence can help develop a potential inhibitor.

Comparative Study on Performances of Composite Anodes of SiO, Si and Graphite for Lithium Rechargeable Batteries

  • Doh, Chil-Hoon;Veluchamy, Angathevar;Lee, Duck-Jun;Lee, Jung-Hoon;Jin, Bong-Soo;Moon, Seong-In;Park, Cheol-Wan;Kim, Dong-Won
    • Bulletin of the Korean Chemical Society
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    • v.31 no.5
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    • pp.1257-1261
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    • 2010
  • The electrochemical performances of anode composites comprising elemental silicon (Si), silicon monoxide (SiO), and graphite (C) were investigated. The composite devoid of elemental silicon (SiO:C = 1:1) and its carbon coated composite showed reduced capacity degradation with measured values of 606 and 584 mAh/g at the fiftieth cycle. The capacity retention nature when the composites were cycled followed the order of Si:SiO:C = 3:1:4 < Si:SiO:C = 2:2:4 < SiO:C = 1:1 < SiO:C = 1:1 (carbon coated). A comparison of the capacity retention properties for the composites in terms of the silicon content showed that a reduced silicon content increased the stability of the composite electrodes. Even though the carbon-coated composite delivered low capacity during cycling compared to the other composites, its low capacity degradation made the anode a better choice for lithium ion batteries.

Feature Detection using Measured 3D Data and Image Data (3차원 측정 데이터와 영상 데이터를 이용한 특징 형상 검출)

  • Kim, Hansol;Jung, Keonhwa;Chang, Minho;Kim, Junho
    • Journal of the Korean Society for Precision Engineering
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    • v.30 no.6
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    • pp.601-606
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    • 2013
  • 3D scanning is a technique to measure the 3D shape information of the object. Shape information obtained by 3D scanning is expressed either as point cloud or as polygon mesh type data that can be widely used in various areas such as reverse engineering and quality inspection. 3D scanning should be performed as accurate as possible since the scanned data is highly required to detect the features on an object in order to scan the shape of the object more precisely. In this study, we propose the method on finding the location of feature more accurately, based on the extended Biplane SNAKE with global optimization. In each iteration, we project the feature lines obtained by the extended Biplane SNAKE into each image plane and move the feature lines to the features on each image. We have applied this approach to real models to verify the proposed optimization algorithm.

A Study on the Development of Cooked Rice according to the Different Coating Ratio of Yacon(Polymia sonchifolia) Root and Its Antioxidant and Sensory Properties (야콘 뿌리 추출액 코팅 쌀 제조 및 항산화력과 관능적 품질 연구)

  • Lee, Ae-Rang
    • The Korean Journal of Food And Nutrition
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    • v.23 no.4
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    • pp.600-606
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    • 2010
  • The objective of this study was to develop functional rice coated with yacon roots extracts and to evaluate the antioxidant properties and physiological activity of rice coated with yacon extract. The washed rice was coated by spraying yacon extract at 10, 20, 30, and $40^{\circ}Brix$ and dried at room temperature. The moisture contents, color changes, antioxidant properties as total polyphenolics and DPPH radical scavenging activity and instrumental texture property and sensory evaluation change in the yacon root extracts coated rice and uncoated cooked rice were observed during 3 days of storage. Increasing the coating concentration was associated with lower color values(L value) and moisture contents. Total phenolics and DPPH radical scavenging properties generally increased in the cooked rice coated with yacon root extract more than the control (non-coated rice). However, D-20($20^{\circ}Brix$ of the yacon root concentration) resulted in better textural properties and sensory overall acceptability in cooked rice on 0 and 3 storage days. It was concluded that the optimum coating ration of rice and added functional yacon rice were $20^{\circ}Brix$ and 7% respectively, in proportion to the total weight of raw rice.