• 한국수산과학회지
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• 제32권1호
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• pp.112-116
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• 1999

톳 포복지의 재생력을 이용하므로써, 수확후 남겨지는 포복지로 부터 재생된 엽체를 양식의 종묘로 재사용하고자 하였다. 엽체 채취후 남겨진 포복지를 연승과 함께 수거하여, 1995년 5월부터 11월까지 조간대의 인공 노출 조건 (1일 0, 1, 2, 3시간)에서 월하관리하여, 엽체의 재생 여부를 실험하였다. 재생엽체의 생장은 엽체의 전장, 주지수 및 중량으로 나타내었다. 생산성 비교를 위하여 재생엽체를 현재 양식방법에서 사용되고 있는 자연산 종묘와 함께 동일 양식장에 이식하여 1995년 12월부터 1996년 5월까지 양성실험을 실시하였다. 생산성 비교 결과 재생엽체와 자연산 유체를 사용한 경우 유의한 차이가 없는 것으로 나타났으나, 포복지의 재사용으로 자연군락으로부터 매년 유체를 채취하지 않고도 계속적인 양식생산을 할 수 있다.

• 한국수산과학회지
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• 제32권1호
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• pp.117-120
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• 1999

해산 녹조류 참흩파래, Monostroma nitidum의 엽체를 효소처리하여 다량의 원형질체를 분리하였다. 최적 효소액의 조합은 $4\%$ R-10+$3\%$ Macerozyme R-10+$3\%$ Abalone acetone power로서 생체조직 300mg 당 $4.41\times10^6$개의 원형질체가 분리되었다. 원형질체의 수율은 효소처리 270분에 최대였다. 분리 직후의 원형질체는 구형으로 직경 $13\~33\mu$m의 크기였다. 분리된 원형질체는 0.4M mannitol을 함유한 f/2배지에서 배양한 후 매주 mannitol이 함유되지 않은 f/2 배지로 절반씩 교환함으로서 분화율을 높일 수 있었다. f/2배지를 사용한 적정 배양조건에서 원형질체는 배양 3일 후 새로운 세포벽을 형성하였으며 10일 후 발아하기 시작하여 엽체로 발달하였다. 항생물질의 배지내 첨가는 배양체의 분화를 저해하였다.

• 환경생물
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• 제31권4호
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• pp.347-352
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• 2013

감태의 인공채묘시 유주자 및 유리배우체를 채묘하여 양성 생장도를 비교하므로써, 유리배우체의 대량양식 이용 가능성을 시험하였다. 2008년 11월에 유주자 및 유리배우체를 채묘한 채묘틀을 이용하여 2009년 5월부터 10월까지 전남 완도군 약산면 시험어장에서 양성 생장도를 비교하였다. 유주자 및 유리배우체 채묘 실험구에서 감태 엽체의 엽장과 생체량 등은 유의한 차이를 보이지 않았다.

• ALGAE
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• 제39권1호
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• pp.17-30
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• 2024

Red macroalga Pyropia yezoensis is a high valuable cultivated marine crop. Its acclimation to cold stress is especially important for long cultivation period across winter in coasts of warm temperate zone in East Asia. In this study, the response of P. yezoensis thalli to low temperature was analyzed on physiology and transcriptome level, to explore its acclimation mechanism to cold stress. The results showed that the practical photosynthesis activity (indicated by Φ_PSII and qP) was depressed and pigment allophycocyanin content was decreased during the cold stress of 48 h. However, the F_v/F_m and non-photochemical quenching increased significantly after 24 h, and the average growth rate of thalli also rebounded from 24 to 48 h, indicating a certain extent of acclimation to cold stress. On transcriptionally, the low temperature promoted the expression of differentially expressed genes (DEGs) related to carbohydrate metabolism and energy metabolism, while genes related to photosynthetic system were depressed. The increased expression of DEGs involved in ribosomal biogenesis and lipid metabolism which could accelerate protein synthesis and enhance the degree of fatty acid unsaturation, might help P. yezoensis thallus cells to cope with cold stress. Further co-expression network analysis revealed differential expression trends along with stress time, and corresponding hub genes play important roles in the systemic acquired acclimation to cold stress. This study provides basic mechanisms of P. yezoensis acclimation to cold temperature and may aid in exploration of functional genes for genetic breeding of economic macroalgae.

• Fisheries and Aquatic Sciences
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• 제4권4호
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• pp.257-260
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• 2001

The effects of light, desiccation and salinity on the discharge of spores in Korean agarophyte, Gracilaria verrucosa were studied. Among the examined factors, light after darkness was the most effective for spore discharge. The maximum release of tetraspores was induced at 24 h after the treatment. Desiccation also seems to be conductive to the release of tetraspores. However, its effect, as in treatment of distilled water for salinity, was hardly found in induction of carpospore discharge. This may suggest that spore discharge in this alga is primarily related with photoperiodic rhythm. Also it appears that the amount of light energy received by fertile thalli also significantly affects to the spore release, considering relationship between the amount of the discharged spores and the elapsed time after treatment.

• ALGAE
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• 제26권3호
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• pp.221-227
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• 2011

There are three carpet-like Codium species with a broadly expanded thallus reported from Korea and Japan: C. latum Suringar, C. tapetum Y. Lee, and C. tenuifolium Shimada, Tadano and J. Tanaka. During surveys of green algal diversity, we encountered a Codium species with carpet-like thalli from several sites on Jeju Island. To confirm the taxonomic identity of these specimens, we studied the morphological features and obtained rbcL gene sequences. The morphological and sequence data indicated that the carpet-like Codium specimens from Jeju are C. tenuifolium from Japan, but distant from Japanese C. latum. We propose that the carpet-like specimens from Jeju should be renamed C. tenuifolium, instead of C. tapetum or C. latum. C. tenuifolium is characterized by a tenuous, carpet-like and erect thallus with a very short and slightly compressed stipe arising from a discal holdfast, sub-pyriform and clavate utricles with a tumid and swollen apical head, and globular or sub-globular gametangia issued at the basal portion of the utricles.

• ALGAE
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• 제29권4호
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• pp.289-297
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• 2014

The taxonomic status and the distribution of Rosenvingea species need attention because of the difficulties in morphological identification in the laboratory as well as in the field. We analyzed mitochondrial cox3 and plastid psaA gene sequences from Rosenvingea species from Vietnam, Mexico and Panama in combination with morphological examinations. Our results confirmed the occurrence of R. intricata and R. orientalis in Vietnam. R. intricata formed dense decumbent mats with many flat branches, often inter-adhesive, and was up to 5 cm in diameter. R. orientalis thalli were up to 18 cm long with narrow (less than 2 mm in width) tubular to compressed fronds. Both cox3 and psaA phylogenies revealed the non-monophyly of the genus Rosenvingea, as reported previously for other scytosiphonacean genera.

• ALGAE
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• 제20권2호
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• pp.99-112
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• 2005

This monographic study presents morphological descriptions of eight species of Padina collected from the Visayas and Mindanao regions of southern Philippines, including distributions of each species and a taxonomic key for all the species examined. Of these species, three are new records for the Philippines, namely: P. fernandeziana Skottsberg and Levring, P. jonesii Tsuda and P. moffittiana Abbott and Huisman. One species, P. antillarum (Kützing) Piccone, represents a new nomenclatural record, which is applied to a Philippine species for the first time. Four species previously reported in the Philippines are reconfirmed and described P. australis Hauck, P. minor Yamada, P. boryana Thivy and P. sanctae-crucis Borgesen. All eight species studied have distromatic thalli, except for P. antillarum which is tetrastromatic. Three of these have indusiate sporangia, namely: P. sanctae-crucis, P. moffittianaand P. fernandeziana.

• Mycobiology
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• 제43권2호
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• pp.157-162
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• 2015

Lichen-forming fungal proteins have been seldom searched due to many difficulties in their extraction. Phenols, quinones, proteases, and other components released during cell disruption have been known to be the greatest challenges related to protein extraction from lichens. To overcome these problems and maintain good electrophoretic resolution and high protein concentration, an extraction buffer containing polyvinylpolypyrrolidone, ascorbic acid, Triton X-100, polyethylene glycol, proteinase, and oxidase inhibitors in sodium phosphate buffer was developed. This extraction buffer showed high efficiency for all lichen species tested in the study.

• ALGAE
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• 제19권4호
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• pp.283-292
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• 2004

Psilothallia is a ceramiaceous red algal. genus that includes three species worldwide: P. dentate, P. siliculosa, and P. striate. The latter two species are limited to Australian waters, and P. dentate occurs in Japan. We here report the detailed morphology of P. dentate, and also determined plastid protein-coding psbA in P. dentate and putative relatives. Psilothallia dentate is distinguished by compressed thalli with alternate-distichous determinate branchlets, six periaxial cells, rhizoidal filaments in axes, cystocarps with 7-8 involucral filaments, spermatangia on branched filaments, and tetrahedrally divided tetrasporangia on branched filaments. Psilothallia dentate is also unusual in that cystocarps, spermatangial clusters, and tetrasporangial tufts are formed on short adventitious indeterminate branches arising on axils of determinate branchlets. The phylogenetic trees of psbA sequences show that P. dentata was nested in a monophyletic Glade comprising Ptilota, Neoptilota, and Plumaria. This result suggests that the taxonomic position of P. dentate may be transferred from the tribe Rhodocallideae to the Ptiloteae.