• Parasites, Hosts and Diseases
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• v.28 no.4
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• pp.207-212
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• 1990

Enzyme-linked immunosorbent assay(ELISA) of paragonimiasis iloktsuenensis rat sera was performed using crude antigens of Paragonimus iloktsuenensis(PIA), P. westermani (PWA) and Clonorchis sinensis(CSA). Three crude antigens(PIA, PWA, CSA) were prepared to saline homogenated supernatants of whole adult worms. Infected rat sera were obtained biweekly from the albino rats fed 50∼.80 metacercariae of P. iloktsuenensis through gastric catheter. Experimental groups were divided into 4 groups: GI(controls), GII, GIII and GIV according to 1∼7 worms as GII, 10∼19 worms as GIII and 22∼40 worms as GIV, respectively, In ELISA, the mean OD values of each group for the homologous antigen(PIA) were increased significantly compared to the control sera at the 4th week of infection. With the progress of duration of infection, the mean OD values of infected sera of GII & GIV continuously increased up to the 12th week(last week), but in GIII the mean OD value increased until the loth week. No significance was noted among the infection dose groups (GII, GIII and GIV), after the 6th week of infection. Also, the OD values of all infected rats did not show any Proportional relytionships to the number of worms recovered. In brief, the antibody productivity of individual rats were strongly different. The rat sera infected with p. iloktsuenensis cross-reacted with those infected with P. westermani or C. sinensis, as identified by OD values.

• Parasites, Hosts and Diseases
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• v.24 no.2
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• pp.187-193
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• 1986

Enzyme-linked immunosorbent assay(ELISA) using crude and affinity-purified antigens of adult worms of Paragonimus westermani was performed for infected cat sera with different worm burden, from preinfection to 18th week after infection. Crude antigen was used with supernatant of homogenated worms by freezing-thawing method, and the supernate was centrifuged for 1 hour at 10,000 rpm at $4^{\circ}C$. Affinity-purified antigen(antibody-bound antigen) was prepared from fractions(bound and unbound) of crude antigen by affinity chromatography on CNBr-activated sepharose 4B, and IgG as a ligand was prepared from paragonimiasis cat serum(6 months infected) obtained by ammonium sulfate ($40%{\sim}45%$ saturated) precipitation method. By SDS-PAGE, crude antigen showed 22 polypeptide fractions while purified antigen showed 4 fractions: 36, 400, 34, 700, 27, 600 and 11, 500 in molecular weights. All cats were divided into five groups($G_1-G_5$) by different worm burdens. The mean of recovered worms(${\pm}SD$) and the number of cats in each group are as follows: $G_1$, 2 worms(0) and 4 cats; $G_2$, 4.75 (${\pm}0.66$) and eight; $G_3$, 10.75(${\pm}1.92$) and four; $G_4$, 23.20(${\pm}3.43$) and five; $G_5$, 48(${\pm}12.63$) and five cats. The results were summarized as follows: 1. The antibody levels(OD value) increased by worm burden in $G_1$ to $G_4$ generally. However, individual antibody levels were not exactly related with worm burden in all groups, especially there was a wide difference in $G_4$ and $G_5$. These results suggested that the worm burden in $G_4$ (about $20{\sim}30$ worms) is enough to produce antibody maximum in cats of $2{\sim}3kg$ weight. 2. The antibody levels increased significantly(p<0.05) compared to control sera at the 3rd week in $G_1$ and $G_2$, at the 2nd week in $G_3$, and at the 1st week in $G_4$ and $G_5$. Especially in the 4th week, OD value increased more in $G_1$(p<0.01) and in $G_2$ to $G_5$(p<0.001). In the pattern of antibody levels by ELISA in each group, OD in $G_1$ increased to the 18th week continuously, in $G_2$ OD was maintained same after the 16th week, but in $G_3$ it decreased after the 16th week, and it was maintained same in $G_4$ and $G_5$ after the 14th week. 3. The antibody levels by ELISA with the affinity-purified antigen were higher than those with crude antigen in all groups generally. Especially, the difference of OD values between two antigens was larger from the 4th to the 10th week. In $G_1$ and $G_2$ OD with purified antigen was higher than that with crude one to the 18th week. It was also higher in $G_3$ than that with crude antigen to the 16th week and OD of $G_4$ and $G_5$ were higher before the 14th week than that with crude antigen, however became lower at the 16th week. Consequently, the antibody level in ELISA with affinity-purified antigen was more sensitive at the early weeks after infection and in light infection groups than that with crude antigen.

• The Journal of Korean Medicine
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• v.23 no.1
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• pp.67-82
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• 2002

Objectives: In recent years, extensive focus has been laid on adulteration of herbal medicine with heavy metals. This may be mainly due to soil contamination by environmental pollution. The objective of this study is to identify the contents of various heavy metals in the blood from Ohjeok-san (Wuji-san) Decoction (OD) treated-rats. Methods: For this study, 13 kinds of metals including essential and heavy metals, i.e. A1, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se and Zn were analyzed by a slight modification of EP A methods and the following results are obtained. Results: 1. There was no significant difference between the OD-treated groups and control group in liver, kidney, bone, brain and weight, especially no significant difference at the 5th and 10th days in weight and the amount of food intake. 2. The amount of each metal analyzed in the blood were as follows; A1: 2.3~3.07 mg/l, As: 2.90~3.66 mg/l, Cd: 0~0.001 mg/l, Co: 0~0.01 mg/l, Cr: 0.40~043 mg/l, Cu: 0.93~1.88 mg/l, Fe: 414.35~464.46 mg/l, Hg: 0.01 mg/l, Mn: 0.10~0.17 mg/l, Ni: 0.01 mg/l, Pb: 0.03~012 mg/l, Se: 0.73 mg/l, Zn: 3.41~4.13 mg/l by groups, respectively. In control and experimental group, Experimental I and other experimental II, III, IV, and V groups, there were no significant differences. 3. The amount of non-toxic metals (A1, Co, Cu, Fe, Mn, Se, Zn) were $64.1{\pm}7.71{\;}mg/l$ in the control group and 60.70~67.58 mg/l in the experimental groups I, II, III, IV and V. The amount of Toxic metals (As, Cd, Cr, Ag, Pb) were $0.68{\pm}0.21{\;}mg/l$ in the control group and 0.57 ~ 0.66mg/l in the experimental groups. The total amount of metals were 32.35 mg/l in the control group and 30.48~34.12 mg/l in the test groups I, II, III, IV and V, respectively. Conclusions: There was no significant difference of metal concentration in the blood from the OD-treated-rats compared to those of the control group even if higher dosage (1~8 times the dosage for a person) of OD was administered. This may be mainly due to a decoction treatment which contains only supernatants filtered from the herb-mass after boiling. This indicates the legal limitation for metal concentration in herbal medicine must be applied according to different treatment methods of herbal medicine.

• Journal of Korean Society on Water Environment
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• v.31 no.1
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• pp.29-34
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• 2015

The aim of this study is to understand the limitation of organic pollutant indexes ($BOD_5$, $COD_{Mn}$, $COD_{Cr}$) and estimate to set the TOC standard by comparing oxidation rates of $BOD_5$, $COD_{Mn}$, $COD_{Cr}$ and TOC based on the 487 organic compounds and 11 effluents from industrial wastewater containing various and unknown organic compounds. The range of ratio of theoretical oxygen demand (ThOD) to theoretical organic carbon (TOCt) was 0.00~5.33 and average ratio of classes of organic compounds was 2.68~3.70. According to classes of organic compounds, the average ratio of $O_2/C$ was 1.2 (range : 1.02~1.39). The order of oxidation rate for 15 organic compounds was TOC (90.7%) > $COD_{Cr}$ (88.8%) > $BOD_5$ (54.4%) > $COD_{Mn}$ (30.8%) indicating the lower oxidation rate of $BOD_5$ and COD compared with TOC. The ranking for average oxidation rate was $COD_{Cr}$ > $COD_{Mn}$ > $BOD_5$ indicating that $BOD_5$, $COD_{Mn}$ could be underestimated comparing with ThOD of organic compounds in case of industrial wastewater containing high concentration of refractory organic compounds. Most of the relationships between organic pollutant indexes and TOC were higher than 0.9. The ratio of TOC to organic compound indexes decreased in the order : $COD_{Cr}$ (3.4) > $COD_{Mn}$ > (1.9) > $BOD_5$ (0.7).

• International Journal of Aeronautical and Space Sciences
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• v.6 no.1
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• pp.71-76
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• 2005

The KOMPSAT-1 satellite, launched into a circular sun synchronous orbit on Dec. 21, 1999, entered its$6^{th}$year of successful operation this year. The purposes of the mission are to collect earth images (6.6 m resolution), multi-spectral images of the ocean, and to collect information on the particle environment of the low earth orbit. For normal operation, KOMPSAT-1 orbits are determined using GPS navigation solutions. However, at the start of the life of KOMPSAT-1, the 11-year solar activity cycle was at a maximum. Solar flux was maintained at this level until 2002, and thereafter reduced to a moderate level by 2004. Thus, the OD (Orbit Determination) accuracy has varied according to the solar activity. This paper presents the degree to which the OD accuracy could be degraded during a high solar activity period compared with that of a (relatively) low solar activity period. We investigated the effect of the use of solve-for parameters such as a drag coefficient ($C_D$), solar radiation coefficient ($C_R$), and the general accelerations ($G_A$) on OD accuracy with solar activity. For the evaluation of orbit determination accuracy, orbit overlap comparison is used since no independent orbits of comparable accuracy are available for comparison. The effect of the use of a box-wing model instead of a constant cross-sectional area is also investigated.

• YAKHAK HOEJI
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• v.43 no.6
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• pp.793-797
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• 1999

Salmonella typhi Ty21a is an attenuated strain of S. typhimurium and used for oral typhoid vaccine. In an attempt to increase the stability of Ty21a manufacturing typhoid vaccine, we studied about the stability of freeze-dried Th21a including additives at various temperature conditions. In order to investigate the freeze-drying rate of Ty21a according to various absorbance, we lyophilized Ty21a by using 8% sucrose as a stabilizer. The optimal freeze-drying rate of Ty21a was appeared when OD (optical density) value of the growth was between 2.5 and 3.0. To investigate the stability of Ty21a at various temperature, the viability was measured after storaging the freeze-dried Ty21a at the room temperature, cold and freezing condition for 1 week. The viability of Ty21a in cold and freezing storage condition was 5 times more stable than in room temperature. To search the most stable additives for the freeze-dried Ty21a, the viability of Ty21a including additives at the various storage condition was estimated. Mannitol and loctose were the most stable additives. Theses results suggest that the OD value of Ty21a growth, low temperature, mannitol and lactose are important factors for the optimal freeze-drying rate, the stable storage and the most stable additives, respectively.

• Journal of Life Science
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• v.8 no.4
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• pp.426-430
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• 1998

The molecular relationships have analyzed between the Bombyx mandarina(wild silkworm) and Bombyx mori strains (domesticated silkworm, geographical silkworms). A total of 166 polymorphic RAPD markers amplified from 35 different primers were used to analyze the molecular relationships among thirteen silkworm strains. The genetic similarity coefficient between Bombyx mandarina and Jam305 showed the lowest genetic similarity value with 0.451, Bombyx mandarina and Bibaekjam showed the highest genetic similarity value with 0.958. These strains were classified into Bombyx mandarina(a wild silkworm) and Bombyx mori(twelve domesticated silkworm) groups upon the genetic similary coefficient of 0.55. Further classificient of 0.60; the 1st sub-group (J111, Bibaekjam, $pnd^{ps}$), the 2nd sub-group (Galwon, C18, od yujam, JAM306, C108), the 3rd sub-group(R-hwang, p50), the 4th sub-group(zebra) and the 5th sub-group(JAM305). According to this study, RAPD markers seems to be a valuable tool for molecular relationships and classification among the silkworms.

• Journal of Biomedical Engineering Research
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• v.6 no.1
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• pp.5-12
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• 1985

In this paper Kalman filtering technique is applied to ultrasound signal to improve resolution capability, Ivhlch is in use of diagnostic imaging systems. The main advantage of Kalman filter algorithm for the analysis of reflected ultrasound signal is its recursive structure which can be easily adapted to tlme varing system. Because soft-tissues, such as liver, act as distributed acoustic low-pass filters which continually change the propagating pulse. tIne can put to practical use above advantage to find a suitable signal generallng model. In state-space description of the system, the 6th order system produces tl)e 1)esc spectral approximation to the source pulse As a result of spectrum analysis, 6th order estimator for two closely spaced ((p.5 mm) reflectors enhances resolution by 4dB-lOdB. By using this result, the possibility to detect even minute tumor is demonstrated.

• Nuclear Engineering and Technology
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• v.45 no.4
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• pp.513-522
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• 2013

The ODSCC detected in the TSP position of Ulchin 3&4 SGs are typical ODSCC of Alloy 600MA tubes. The causative chemical environment is formed by concentration of impurities inside the occluded region formed by the tube surface, egg crate strips, and sludge deposit there. Most cracks are detected at or near the line contacts between the tube surface and the egg crate strips. The region of dense crack population, as defined as between $4^{th}$ and $9^{th}$ TSPs, and near the center of hot leg hemisphere plane, coincided well with the region of preferential sludge deposition as defined by thermal hydraulics calculation using SGAP computer code. The cracks developed homogeneously in a wide range of SGs, so that the number of cracks detected each outage increased very rapidly since the first detection in the $8^{th}$ refueling outage. The root cause assessment focused on investigation of the difference in microstructure and manufacturing residual stress in order to reveal the cause of different susceptibilities to ODSCC among identical six units. The manufacturing residual stress as measured by XRD on OD surface and by split tube method indicated that the high residual stress of Alloy 600MA tube played a critical role in developing ODSCC. The level of residual stress showed substantial variations among the six units depending on details of straightening and OD grinding processes. Youngwang 3&4 tubes are less susceptible to ODSCC than U3 and U4 tubes because semi-continuous coarse chromium carbides are formed along the grain boundary of Y3&4 tubes, while there are finer less continuous chromium carbides in U3 and U4. The different carbide morphology is caused by the difference in cooling rate after mill anneal. There is a possibility that high chromium content in the Y3&4 tubes, still within the allowable range of Alloy 600, has made some contribution to the improved resistance to ODSCC. It is anticipated that ODSCC in Y5&6 SGs will be retarded more considerably than U3 SGs since the manufacturing residual stress in Y5&6 tubes is substantially lower than in U3 tubes, while the microstructure is similar with each other.

• Journal of the Korean Magnetic Resonance Society
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• v.6 no.2
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• pp.84-88
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• 2002

Dating and dosimetry using electron spin resonance (ESR) in 20th Century developed at both Yamaguchi University and Osaka University have been reviewed with emphasis on new prospects and strategies in 21th century. Natural radiation have been generating radicals that accumulated in archaeological and geological materials. ESR detects these radicals and the ESR signal intensity is proportional to the radiation dose and therefore the age. The assessment of the total dose of natural radiation and the annual dose rate give their ESR ages. The ESR dating of stalactites and stalagmites ant Akiyoshi cave in Yamaguchi prefecture in 1975 was extended to anthropological dating using bones and tooth enamel excavated in Greek Petralona cave. Fossils of shells and corals gave the ages of marine terraces and sea-level changes. Quartz grains gave the ages of geothermal alteration and fault movements. Future ESR dating of ices at outer planets anf their satellite are also investigated as basic studies for ices od $H_2O,\;CO_2,\;SO_2$ as well as terrestrial hydrates in laboratory. Atomic bomb radiation dosimetry at Hiroshima and Nagasaki using ESR lead to the dosimetry of personnel, Chemobyl and JCO criticality accidents. Monitoring of radiation dose with sensitive materials with tissue equivalence are being developed. finally a new scanning ESR imaging apparatus (a near field microwave microscope) developed in our laboratory gave ESR images of Radicals from fossils to Si-CVD and diamond films as summarized in my book in 2002.